Latest content added for UNT Digital Library Searchhttps://digital2.library.unt.edu/search/?t=fulltext&fq=untl_institution%3AUNTGD&sort=added_d2024-03-28T07:50:46-05:00UNT LibrariesThis is a custom feed for searching UNT Digital Library SearchEcoregions of Texas [Poster]2024-03-28T07:50:46-05:00https://texashistory.unt.edu/ark:/67531/metapth1662409/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth1662409/"><img alt="Ecoregions of Texas [Poster]" title="Ecoregions of Texas [Poster]" src="https://texashistory.unt.edu/ark:/67531/metapth1662409/thumbnail/"/></a></p><p>Poster containing a map color-coded to "denote areas of general similarity in ecosystems and in the type, quality and quantity of environmental resources" with a key to regions and sub-regions in the lower-left corner along with a similar inset for the conterminous United States (scale 1:2,500,000). The poster also includes a list of the regions in Texas with descriptions and illustrative photographs to the right of the map, which is continued on the back. The back of the poster also includes a smaller version of the state map in the lower-left corner along with a list of common and scientific names of native plants and and wildlife, as well as a source list.</p>Symbols of Service: Wear Them Proudly & Properly!2023-03-08T07:31:21-06:00https://digital.library.unt.edu/ark:/67531/metadc2061540/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc2061540/"><img alt="Symbols of Service: Wear Them Proudly & Properly!" title="Symbols of Service: Wear Them Proudly & Properly!" src="https://digital.library.unt.edu/ark:/67531/metadc2061540/thumbnail/"/></a></p><p>Poster which "represents in correct order of precedence, ribbons and devices most likely to be worn by members of today's Air Force" (top of poster), with an image and label for each.</p>Meals are a great time to play FOLLOW-THE-LEADER: and you're the leader2019-01-31T21:03:17-06:00https://texashistory.unt.edu/ark:/67531/metapth1115044/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth1115044/"><img alt="Meals are a great time to play FOLLOW-THE-LEADER: and you're the leader" title="Meals are a great time to play FOLLOW-THE-LEADER: and you're the leader" src="https://texashistory.unt.edu/ark:/67531/metapth1115044/thumbnail/"/></a></p><p>Poster discussing fostering healthy eating habits in children by parents modeling healthy eating. The front of the poster shows to hands holding bananas.</p>Healthy Plate (Pre-diabetes and diabetes)2019-01-31T21:03:17-06:00https://texashistory.unt.edu/ark:/67531/metapth1114757/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth1114757/"><img alt="Healthy Plate (Pre-diabetes and diabetes)" title="Healthy Plate (Pre-diabetes and diabetes)" src="https://texashistory.unt.edu/ark:/67531/metapth1114757/thumbnail/"/></a></p><p>Poster depicting a plate showing the proportions of different types of food as portions of a healthy meal for a person with pre-diabetes or diabetes. The back of the poster is a chart showing the carbohydrate content of various foods.</p>Putting on weight with healthy foods makes taking it off a LOT easier2019-01-31T21:03:17-06:00https://texashistory.unt.edu/ark:/67531/metapth1114704/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth1114704/"><img alt="Putting on weight with healthy foods makes taking it off a LOT easier" title="Putting on weight with healthy foods makes taking it off a LOT easier" src="https://texashistory.unt.edu/ark:/67531/metapth1114704/thumbnail/"/></a></p><p>Poster discussing healthy eating during pregnancy to support a healthy pregnancy. The front of the poster depicts a pregnant woman wearing a green sweater resting her hands on her belly.</p>Top 10 ways to grow happy kids2019-01-31T21:03:17-06:00https://texashistory.unt.edu/ark:/67531/metapth1113903/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth1113903/"><img alt="Top 10 ways to grow happy kids" title="Top 10 ways to grow happy kids" src="https://texashistory.unt.edu/ark:/67531/metapth1113903/thumbnail/"/></a></p><p>Poster with a photograph of four children laying on grass and listing ten ways to encourage healthy habits in children, focusing on healthy eating.</p>After a year, its time to use those bottles for something else2019-01-31T21:03:17-06:00https://texashistory.unt.edu/ark:/67531/metapth1114717/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth1114717/"><img alt="After a year, its time to use those bottles for something else" title="After a year, its time to use those bottles for something else" src="https://texashistory.unt.edu/ark:/67531/metapth1114717/thumbnail/"/></a></p><p>Poster discussing how extended bottlefeeding can damage a child's dental health and tips to reduce bottlefeeding.</p>Happiness Grows Here!2019-01-31T21:03:17-06:00https://texashistory.unt.edu/ark:/67531/metapth1114665/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth1114665/"><img alt="Happiness Grows Here!" title="Happiness Grows Here!" src="https://texashistory.unt.edu/ark:/67531/metapth1114665/thumbnail/"/></a></p><p>Poster with the text, "Happiness Grows Here!" printed in the center with illustrated plants to the right of of the text.</p>Breast Milk every ounce counts2019-01-31T21:03:17-06:00https://texashistory.unt.edu/ark:/67531/metapth1114154/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth1114154/"><img alt="Breast Milk every ounce counts" title="Breast Milk every ounce counts" src="https://texashistory.unt.edu/ark:/67531/metapth1114154/thumbnail/"/></a></p><p>Poster of a woman wearing a lab coat with the text, "As a doctor i know breastfeeding is the healthiest thing you can do for your baby. As a mother i feel it is the most beautiful." printed at the top of the poster.</p>Ganar peso con alimentos saludables hace que perderlo sea MUCHO más fácil2019-01-31T21:03:17-06:00https://texashistory.unt.edu/ark:/67531/metapth1114934/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth1114934/"><img alt="Ganar peso con alimentos saludables hace que perderlo sea MUCHO más fácil" title="Ganar peso con alimentos saludables hace que perderlo sea MUCHO más fácil" src="https://texashistory.unt.edu/ark:/67531/metapth1114934/thumbnail/"/></a></p><p>Poster about the importance of having a healthy diet during pregnancy, also the implications that it takes. El póster es acerca la importancia de una dieta saludable durante el embarazo, también las implicaciones que lleva.</p>Keeping their spirits up helps keep their weight down2019-01-31T21:03:17-06:00https://texashistory.unt.edu/ark:/67531/metapth1115218/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth1115218/"><img alt="Keeping their spirits up helps keep their weight down" title="Keeping their spirits up helps keep their weight down" src="https://texashistory.unt.edu/ark:/67531/metapth1115218/thumbnail/"/></a></p><p>None</p>Give your child 10 tasteful GIFTS that will last a lifetime2019-01-31T21:03:17-06:00https://texashistory.unt.edu/ark:/67531/metapth1115315/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth1115315/"><img alt="Give your child 10 tasteful GIFTS that will last a lifetime" title="Give your child 10 tasteful GIFTS that will last a lifetime" src="https://texashistory.unt.edu/ark:/67531/metapth1115315/thumbnail/"/></a></p><p>Poster discussing healthy foods and tips for making them appealing to children.</p>Texas State Park Rules and Regulations2019-01-31T21:03:17-06:00https://texashistory.unt.edu/ark:/67531/metapth1114840/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth1114840/"><img alt="Texas State Park Rules and Regulations" title="Texas State Park Rules and Regulations" src="https://texashistory.unt.edu/ark:/67531/metapth1114840/thumbnail/"/></a></p><p>Poster listing Texas Sate Park's rules and regulations.</p>Right Now, You're Helping the Health of the Baby You're Carrying2019-01-31T21:03:17-06:00https://texashistory.unt.edu/ark:/67531/metapth1113832/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth1113832/"><img alt="Right Now, You're Helping the Health of the Baby You're Carrying" title="Right Now, You're Helping the Health of the Baby You're Carrying" src="https://texashistory.unt.edu/ark:/67531/metapth1113832/thumbnail/"/></a></p><p>2 posters promoting the importance of regular doctor visits and listing questions for women to ask their doctors about their health. The first poster is aimed at pregnant women and includes questions about breastfeeding, relationships, and birth plans. The second poster is aimed at women hoping to become pregnant and includes questions about lifestyle, medical history, and general health.</p>Las 10 mejores maneras de criar niños felices2019-01-31T21:03:17-06:00https://texashistory.unt.edu/ark:/67531/metapth1114075/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth1114075/"><img alt="Las 10 mejores maneras de criar niños felices" title="Las 10 mejores maneras de criar niños felices" src="https://texashistory.unt.edu/ark:/67531/metapth1114075/thumbnail/"/></a></p><p>Poster over the healthiest methods to raise children. Póster sobre los metodos mas saludables para criar niños.</p>To Your Child, You Are the Greatest Show on Earth2019-01-31T21:03:17-06:00https://texashistory.unt.edu/ark:/67531/metapth1115276/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth1115276/"><img alt="To Your Child, You Are the Greatest Show on Earth" title="To Your Child, You Are the Greatest Show on Earth" src="https://texashistory.unt.edu/ark:/67531/metapth1115276/thumbnail/"/></a></p><p>Poster of a small child wearing a gingham dress and clapping her hands and discussing the importance of playing with children.</p>Texas Agriculture Matters2019-01-31T21:03:17-06:00https://texashistory.unt.edu/ark:/67531/metapth1115005/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth1115005/"><img alt="Texas Agriculture Matters" title="Texas Agriculture Matters" src="https://texashistory.unt.edu/ark:/67531/metapth1115005/thumbnail/"/></a></p><p>Pamphlet with information about Texas agriculture. One side has a photo of a fence around a field with the Texas flag painted on the gate, and the other side has a collage of facts about the history and current state of Texas agriculture.</p>Breastmilk: Baby's First Immunization, 20112018-07-31T12:13:18-05:00https://texashistory.unt.edu/ark:/67531/metapth1031853/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth1031853/"><img alt="Breastmilk: Baby's First Immunization, 2011" title="Breastmilk: Baby's First Immunization, 2011" src="https://texashistory.unt.edu/ark:/67531/metapth1031853/thumbnail/"/></a></p><p>Image with short graphs describing the benefits of immunization by maternal breastmilk feeding for infants.</p>Fregadero de Tres Compartimientos2017-12-07T22:12:14-06:00https://texashistory.unt.edu/ark:/67531/metapth903526/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth903526/"><img alt="Fregadero de Tres Compartimientos" title="Fregadero de Tres Compartimientos" src="https://texashistory.unt.edu/ark:/67531/metapth903526/thumbnail/"/></a></p><p>Poster depicting a three-compartment sink labelled with the purpose of each area. There is also information about sanitizing solutions and minimum required temperatures to meet Texas Health Department regulations.</p>Three-Compartment Sink2017-12-07T07:46:27-06:00https://texashistory.unt.edu/ark:/67531/metapth903781/<p><a href="https://texashistory.unt.edu/ark:/67531/metapth903781/"><img alt="Three-Compartment Sink" title="Three-Compartment Sink" src="https://texashistory.unt.edu/ark:/67531/metapth903781/thumbnail/"/></a></p><p>Poster depicting a three-compartment sink labelled with the purpose of each area. There is also information about sanitizing solutions and minimum required temperatures to meet Texas Health Department regulations.</p>Taxa-area Relationship (TAR) of Microbial Functional Genes with Long-TGerm Fertilization2017-10-14T08:36:47-05:00https://digital.library.unt.edu/ark:/67531/metadc1012638/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc1012638/"><img alt="Taxa-area Relationship (TAR) of Microbial Functional Genes with Long-TGerm Fertilization" title="Taxa-area Relationship (TAR) of Microbial Functional Genes with Long-TGerm Fertilization" src="https://digital.library.unt.edu/ark:/67531/metadc1012638/thumbnail/"/></a></p><p>Diversity and spatial patterns in plant and animal communities are well documented as a positive-power law of a taxa-area relationship (TAR). At present little is known whether this also applies to soil microbial communities and whether long-term fertilization has an influence on the underlying microbial diversity. To test the effects of long-term fertilization on above-ground botanical diversity and below-ground microbial diversity, a nested sampling approach on Park Grass plots (12d& 11/2c) of Rothamsted Reseach in United Kingdom, both at ~;; pH 5 but with plant diversities of between 42 and 13 respectively were used. GeoChip 3.0, covering approximately 57, 000 gene sequences of 292 gene families involved in nitrogen, carbon, sulfur and phosphorus cycling, metal reduction and resistance, and organic contaminant degradation, was used to determine the gene area relationships for both functional and phylogenetic groups and the relationship to plant diversity. Our analysis indicated that the microbial communities were separated by different plant diversity based on DCA. The soil microbial diversity was in accord with plant diversity. Soil microbial community exhibited different z value with different plant diversity, z = 0.0449 with higher plant diversity and z = 0.0583 with lower plant diversity (P< 0.0001). These results suggest that the turnover in space of microorganisms may be higher with long-term fertilization.</p>Functional Ecological Gene Networks to Reveal the Changes Among Microbial Interactions Under Elevated Carbon Dioxide Conditions2017-10-14T08:36:47-05:00https://digital.library.unt.edu/ark:/67531/metadc1012652/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc1012652/"><img alt="Functional Ecological Gene Networks to Reveal the Changes Among Microbial Interactions Under Elevated Carbon Dioxide Conditions" title="Functional Ecological Gene Networks to Reveal the Changes Among Microbial Interactions Under Elevated Carbon Dioxide Conditions" src="https://digital.library.unt.edu/ark:/67531/metadc1012652/thumbnail/"/></a></p><p>Biodiversity and its responses to environmental changes is a central issue in ecology, and for society. Almost all microbial biodiversity researches focus on species richness and abundance but ignore the interactions among different microbial species/populations. However, determining the interactions and their relationships to environmental changes in microbial communities is a grand challenge, primarily due to the lack of information on the network structure among different microbial species/populations. Here, a novel random matrix theory (RMT)-based conceptual framework for identifying functional ecological gene networks (fEGNs) is developed with the high throughput functional gene array hybridization data from the grassland microbial communities in a long-term FACE (Free Air CO2 Enrichment) experiment. Both fEGNs under elevated CO2 (eCO2) and ambient CO2 (aCO2) possessed general characteristics of many complex systems such as scale-free, small-world, modular and hierarchical. However, the topological structure of the fEGNs is distinctly different between eCO2 and aCO2, suggesting that eCO2 dramatically altered the interactions among different microbial functional groups/populations. In addition, the changes in network structure were significantly correlated with soil carbon and nitrogen dynamics, and plant productivity, indicating the potential importance of network interactions in ecosystem functioning. Elucidating network interactions in microbial communities and their responses to environmental changes are fundamentally important for research in microbial ecology, systems microbiology, and global change.</p>The Role of the Tetraheme Cytochrome c3 in Desulfovibrio vulgaris Hildenborough Metabolism2017-10-14T08:36:47-05:00https://digital.library.unt.edu/ark:/67531/metadc1014507/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc1014507/"><img alt="The Role of the Tetraheme Cytochrome c3 in Desulfovibrio vulgaris Hildenborough Metabolism" title="The Role of the Tetraheme Cytochrome c3 in Desulfovibrio vulgaris Hildenborough Metabolism" src="https://digital.library.unt.edu/ark:/67531/metadc1014507/thumbnail/"/></a></p><p>The role of tetraheme cytochrome c3 (CycA) in the metabolism of the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough (DvH) was investigated by deletion of the cycA gene using a marker-exchange deletion strategy. A highly abundant periplasmic cytochrome, CycA has the important function of transferring electrons from periplasmic hydrogenases (Hyd, Hyn, Hys) to transmembrane complexes which transport the electrons to the cytoplasm where sulfate is reduced. Previous studies have indicated that during its interaction with periplasmic hydrogenases, CycA is also involved in the reduction of toxic metals. Growth of the cycA mutant strain on lactate as the electron donor and sulfate as the terminal electron acceptor showed that, despite its abundance, CycA is not essential for DvH growth. However, the rate of growth of the mutant strain was significantly lower, and the extent of growth less, than rates and extents of growth of the wild type and complement strains on lactate/sulfate medium. This indicates that a portion of the electrons generated from cytoplasmic lactate oxidation are transported by CycA for energy production, possibly in a hydrogen cycling mechanism employed to generate ATP. Failure of the mutant strain to grow on either formate or H2, with sulfate or sulfite as electron acceptors, further indicated that CycA may be the only redox partner of periplasmic hydrogenases. The cycA mutant strain also did not grow as well as either the wild type or complement strains on medium supplemented with pyruvate/sulfate. Final growth on pyruvate/sulfate was comparable, but the mutant grew more slowly than the wild type and complement strains. Interestingly, the mutant grew better than the wild type or complement strains on pyruvate alone, possibly due to the release of H2 and/or CO2 in concentrations which may be somewhat inhibitory to wild type growth.</p>Effects of experimental warming and clipping on metabolic change of microbial community in a US Great Plains tallgrass prairie2017-10-14T08:36:47-05:00https://digital.library.unt.edu/ark:/67531/metadc1014989/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc1014989/"><img alt="Effects of experimental warming and clipping on metabolic change of microbial community in a US Great Plains tallgrass prairie" title="Effects of experimental warming and clipping on metabolic change of microbial community in a US Great Plains tallgrass prairie" src="https://digital.library.unt.edu/ark:/67531/metadc1014989/thumbnail/"/></a></p><p>While more and more studies are being conducted on the effects of global warming, little is known regarding the response of metabolic change of whole soil microbial communities to this phenomenon. In this study, functional gene changes at the mRNA level were analyzed by our new developed GeoChip 3.0. Soil samples were taken from a long-term climate warming experiment site, which has been conducted for ~;;8 years at the Kessler Farm Field Laboratory, a 137.6-ha farm located in the Central Redbed Plains, in McClain County, Oklahoma. The experiment uses a paired factorial design with warming as the primary factor nested with clipping as a secondary factor. An infrared heater was used to simulate global warming, and clipping was used to mimic mowing hay. Twelve 2m x 2m plots were divided into six pairs of warmed and control plots. The heater generates a constant output of ~;;100 Watts m-2 to approximately 2 oC increase in soil temperature above the ambient plots, which is at the low range of the projected climate warming by IPCC. Soil whole microbial communities? mRNA was extracted, amplified, labeled and hybridized with our GeoChip 3.0, a functional gene array covering genes involved in N, C, P, and S cycling, metal resistance and contaminant degradation, to examine expressed genes. The results showed that a greater number and higher diversity of genes were expressed under warmed plots compared to control. Detrended correspondence analysis (DCA) of all detected genes showed that the soil microbial communities were clearly altered by warming, with or without clipping. The dissimilarity of the communities based on functional genes was tested and results showed that warming and control communities were significantly different (P<0.05), with or without clipping. Most genes involved in C, N, P and S cycling were expressed at higher levels in warming samples compared to control samples. All of the results demonstrated that the whole microbial communities increase functional gene expression under warming with or without clipping in order to adapt the changed out environment. More detail analysis is underway.</p>Investigating the role of CheA-3 in Dusulfovibrio Vulgaris Hildenborough2017-10-14T08:36:47-05:00https://digital.library.unt.edu/ark:/67531/metadc1014526/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc1014526/"><img alt="Investigating the role of CheA-3 in Dusulfovibrio Vulgaris Hildenborough" title="Investigating the role of CheA-3 in Dusulfovibrio Vulgaris Hildenborough" src="https://digital.library.unt.edu/ark:/67531/metadc1014526/thumbnail/"/></a></p><p>Multiple sets of chemotaxis genes including three cheA homologs were identified in the genome sequence of the anaerobic bacterium Desulfovibrio vulgaris Hildenborough. Each CheA is a histidine kinase (HK) and part of a two component signal transduction system. Knock out mutants in the three cheA genes were created using single cross-over homologous recombination insertion. We studied the phenotypes of the cheA mutants in detail and discovered that ?cheA-3 has a non swarming/swimming phenotype both in the soft agar plates and Palleroni chamber assays. CheA-3 shows similarity to the Shewanella oneidensis CheA-3 and the Vibrio cholerae CheA-2 that are responsible for chemotaxis in the respective organisms. We did not find any morphological or structural differences between the three Delta cheA mutants and the wild type cells in electron microscopy. Our results from these studies are presented.</p>Identification of Small RNAs in Desulfovibrio vulgaris Hildenborough2017-10-14T08:36:47-05:00https://digital.library.unt.edu/ark:/67531/metadc1012624/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc1012624/"><img alt="Identification of Small RNAs in Desulfovibrio vulgaris Hildenborough" title="Identification of Small RNAs in Desulfovibrio vulgaris Hildenborough" src="https://digital.library.unt.edu/ark:/67531/metadc1012624/thumbnail/"/></a></p><p>Desulfovibrio vulgaris is an anaerobic sulfate-reducing bacterium capable of facilitating the removal of toxic metals such as uranium from contaminated sites via reduction. As such, it is essential to understand the intricate regulatory cascades involved in how D. vulgaris and its relatives respond to stressors in such sites. One approach is the identification and analysis of small non-coding RNAs (sRNAs); molecules ranging in size from 20-200 nucleotides that predominantly affect gene regulation by binding to complementary mRNA in an anti-sense fashion and therefore provide an immediate regulatory response. To identify sRNAs in D. vulgaris, a bacterium that does not possess an annotated hfq gene, RNA was pooled from stationary and exponential phases, nitrate exposure, and biofilm conditions. The subsequent RNA was size fractionated, modified, and converted to cDNA for high throughput transcriptomic deep sequencing. A computational approach to identify sRNAs via the alignment of seven separate Desulfovibrio genomes was also performed. From the deep sequencing analysis, 2,296 reads between 20 and 250 nt were identified with expression above genome background. Analysis of those reads limited the number of candidates to ~;;87 intergenic, while ~;;140 appeared to be antisense to annotated open reading frames (ORFs). Further BLAST analysis of the intergenic candidates and other Desulfovibrio genomes indicated that eight candidates were likely portions of ORFs not previously annotated in the D. vulgaris genome. Comparison of the intergenic and antisense data sets to the bioinformatical predicted candidates, resulted in ~;;54 common candidates. Current approaches using Northern analysis and qRT-PCR are being used toverify expression of the candidates and to further develop the role these sRNAs play in D. vulgaris regulation.</p>Mapping the Two-component Regulatory Networks in Desulfovibrio vulgaris2017-10-14T08:36:47-05:00https://digital.library.unt.edu/ark:/67531/metadc1013270/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc1013270/"><img alt="Mapping the Two-component Regulatory Networks in Desulfovibrio vulgaris" title="Mapping the Two-component Regulatory Networks in Desulfovibrio vulgaris" src="https://digital.library.unt.edu/ark:/67531/metadc1013270/thumbnail/"/></a></p><p>D. vulgaris Hildenborough has 72 response regulators. The Desulfovibrio are sulfate reducing bacteria that are important in the sulfur and carbon cycles in anoxic habitats. Its large number of two componenent systems are probably critical to its ability to sense and respond to its environment. Our goal is to map these RRs to the genes they regulate using a DNA-affinity-purification-chip (DAP-chip) protocol. First target determined usuing EMSA. A positive target was determined for as many RRs as possible using EMSA. Targets were selected based on gene proximity, regulon predictions and/or predicted sigma54 dependent promoters. qPCR was used to ensure that the target was enriched from sheared genomic DNA before proceeding to the DAP-chip.</p>Syntrophic Degradation of Lactate in Methanogenic Co-cultures2017-10-14T08:36:47-05:00https://digital.library.unt.edu/ark:/67531/metadc1013310/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc1013310/"><img alt="Syntrophic Degradation of Lactate in Methanogenic Co-cultures" title="Syntrophic Degradation of Lactate in Methanogenic Co-cultures" src="https://digital.library.unt.edu/ark:/67531/metadc1013310/thumbnail/"/></a></p><p>In environments where the amount of the inorganic electron acceptors (oxygen, nitrate, sulfate, sulfur oroxidized metal ions (Fe3+;Mn4+) is insufficient for complete breakdown of organic matter, methane is formed as the major reduced end product. In such methanogenic environments organic acids are degraded by syntrophic associations of fermenting, acetogenic bacteria (e.g., sulfate-reducing bacteria (SRB) as"secondary fermenters") and methanogenic archaea. In these consortia, the conversion of lactate to acetate, CO2 and methane depends on the cooperating activities of both metabolically distinct microbial groups that are tightly linked by the need to maintain the exchanged metabolites (hydrogenandformate) at very low concentrations.</p>Two Component Signal Transduction in Desulfovibrio Species2017-10-14T08:36:47-05:00https://digital.library.unt.edu/ark:/67531/metadc1015535/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc1015535/"><img alt="Two Component Signal Transduction in Desulfovibrio Species" title="Two Component Signal Transduction in Desulfovibrio Species" src="https://digital.library.unt.edu/ark:/67531/metadc1015535/thumbnail/"/></a></p><p>The environmentally relevant Desulfovibrio species are sulfate-reducing bacteria that are of interest in the bioremediation of heavy metal contaminated water. Among these, the genome of D. vulgaris Hildenborough encodes a large number of two component systems consisting of 72 putative response regulators (RR) and 64 putative histidinekinases (HK), the majority of which are uncharacterized. We classified the D. vulgaris Hildenborough RRs based on their output domains and compared the distribution of RRs in other sequenced Desulfovibrio species. We have successfully purified most RRs and several HKs as His-tagged proteins. We performed phospho-transfer experiments to verify relationships between cognate pairs of HK and RR, and we have also mapped a few non-cognate HK-RR pairs. Presented here are our discoveries from the Desulfovibrio RR categorization and results from the in vitro studies using purified His tagged D. vulgaris HKs and RRs.</p>Phylogenetic Analysis of Shewanella Strains by DNA Relatedness Derived from Whole Genome Microarray DNA-DNA Hybridization and Comparison with Other Methods2017-10-14T08:36:47-05:00https://digital.library.unt.edu/ark:/67531/metadc1015185/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc1015185/"><img alt="Phylogenetic Analysis of Shewanella Strains by DNA Relatedness Derived from Whole Genome Microarray DNA-DNA Hybridization and Comparison with Other Methods" title="Phylogenetic Analysis of Shewanella Strains by DNA Relatedness Derived from Whole Genome Microarray DNA-DNA Hybridization and Comparison with Other Methods" src="https://digital.library.unt.edu/ark:/67531/metadc1015185/thumbnail/"/></a></p><p>Phylogenetic analyses were done for the Shewanella strains isolated from Baltic Sea (38 strains), US DOE Hanford Uranium bioremediation site [Hanford Reach of the Columbia River (HRCR), 11 strains], Pacific Ocean and Hawaiian sediments (8 strains), and strains from other resources (16 strains) with three out group strains, Rhodopseudomonas palustris, Clostridium cellulolyticum, and Thermoanaerobacter ethanolicus X514, using DNA relatedness derived from WCGA-based DNA-DNA hybridizations, sequence similarities of 16S rRNA gene and gyrB gene, and sequence similarities of 6 loci of Shewanella genome selected from a shared gene list of the Shewanella strains with whole genome sequenced based on the average nucleotide identity of them (ANI). The phylogenetic trees based on 16S rRNA and gyrB gene sequences, and DNA relatedness derived from WCGA hybridizations of the tested Shewanella strains share exactly the same sub-clusters with very few exceptions, in which the strains were basically grouped by species. However, the phylogenetic analysis based on DNA relatedness derived from WCGA hybridizations dramatically increased the differentiation resolution at species and strains level within Shewanella genus. When the tree based on DNA relatedness derived from WCGA hybridizations was compared to the tree based on the combined sequences of the selected functional genes (6 loci), we found that the resolutions of both methods are similar, but the clustering of the tree based on DNA relatedness derived from WMGA hybridizations was clearer. These results indicate that WCGA-based DNA-DNA hybridization is an idea alternative of conventional DNA-DNA hybridization methods and it is superior to the phylogenetics methods based on sequence similarities of single genes. Detailed analysis is being performed for the re-classification of the strains examined.</p>Microarray-based analysis of survival of soil microbial community during ozonation2017-10-14T08:36:47-05:00https://digital.library.unt.edu/ark:/67531/metadc1015228/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc1015228/"><img alt="Microarray-based analysis of survival of soil microbial community during ozonation" title="Microarray-based analysis of survival of soil microbial community during ozonation" src="https://digital.library.unt.edu/ark:/67531/metadc1015228/thumbnail/"/></a></p><p>A 15 h ozonation was performed on bioremediated soil to remove recalcitrant residual oil. To monitor the survival of indigenous microorganisms in the soil during in-situ chemical oxidation(ISCO) culturing and a functional genearray, GeoChip, was used to examine the functional genes and structure of the microbial community during ozonation (0h, 2h, 4h, 6h, 10hand15h). Breakthrough ozonation decreased the population of cultivable heterotrophic bacteria by about 3 orders of magnitude. The total functional gene abundance and diversity decreased during ozonation, as the number of functional genes was reduced by 48percent after 15 h. However, functional genes were evenly distributed during ozonation as judged by the Shannon-Weaver Evenness index. A sharp decrease in gene number was observed in the first 6 h of ozonation followed by a slower decrease in the next 9 h, which was consistent with microbial populations measured by a culture based method. Functional genes involved in carbon, nitrogen, phosphors and sulfur cycling, metal resistance and organic remediation were detected in all samples. Though the pattern of gene categories detected was similar for all time points, hierarchica lcluster of all functional genes and major functional categories all showed a time-serial pattern. Bacteria, archaea and fungi decreased by 96.1percent, 95.1percent and 91.3percent, respectively, after 15 h ozonation. Delta proteobacteria, which were reduced by 94.3percent, showed the highest resistance to ozonation while Actinobacteria, reduced by 96.3percent, showed the lowest resistance. Microorganisms similar to Rhodothermus, Obesumbacterium, Staphylothermus, Gluconobacter, and Enterococcus were dominant at all time points. Functional genes related to petroleum degradation decreased 1~;;2 orders of magnitude. Most of the key functional genes were still detected after ozonation, allowing a rapid recovery of the microbial community after ozonation. While ozone had a large impact on the indigenous soil microorganisms, a fraction of the key functional gene-containing microorganisms survived during ozonation and kept the community functional.</p>Hispanic Women in the Labor Force2017-02-20T06:21:05-06:00https://digital.library.unt.edu/ark:/67531/metadc955751/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc955751/"><img alt="Hispanic Women in the Labor Force" title="Hispanic Women in the Labor Force" src="https://digital.library.unt.edu/ark:/67531/metadc955751/thumbnail/"/></a></p><p>Infographic showing statistics pertaining to Hispanic women employment in 2015. The pamphlet compares wages and conditions of Hispanic women with women of other races.</p>Working Mothers in the U.S.2017-02-20T06:21:05-06:00https://digital.library.unt.edu/ark:/67531/metadc955615/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc955615/"><img alt="Working Mothers in the U.S." title="Working Mothers in the U.S." src="https://digital.library.unt.edu/ark:/67531/metadc955615/thumbnail/"/></a></p><p>Infographic of working mothers in the United States based upon data from 2012 American Community Survey, U.S. Census Bureau (ACS), Pew Research Center Analysis of Decennial Census (2011), and the Bureau of Labor Statistics, Current Population Survey.</p>50 Years Later: Women, Work & the Work Ahead2017-02-20T06:21:05-06:00https://digital.library.unt.edu/ark:/67531/metadc955360/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc955360/"><img alt="50 Years Later: Women, Work & the Work Ahead" title="50 Years Later: Women, Work & the Work Ahead" src="https://digital.library.unt.edu/ark:/67531/metadc955360/thumbnail/"/></a></p><p>Infographic commemorating the 50th anniversary of the American Women Report by outlining statistics and major events related to women's education and participation in the workforce from 1963 to 2012.</p>Equal Pay2017-01-28T09:27:04-06:00https://digital.library.unt.edu/ark:/67531/metadc949068/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc949068/"><img alt="Equal Pay" title="Equal Pay" src="https://digital.library.unt.edu/ark:/67531/metadc949068/thumbnail/"/></a></p><p>Infographic providing information on the inequity of pay between women and men in the United States job market.</p>Effectiveness of Family Planning Methods2017-01-28T09:27:04-06:00https://digital.library.unt.edu/ark:/67531/metadc949015/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc949015/"><img alt="Effectiveness of Family Planning Methods" title="Effectiveness of Family Planning Methods" src="https://digital.library.unt.edu/ark:/67531/metadc949015/thumbnail/"/></a></p><p>Single page chart with graphical information on a variety of contraception methods and their percentage of effectiveness.</p>Synchrotron-based high-pressure research in materials science2016-11-13T19:26:23-06:00https://digital.library.unt.edu/ark:/67531/metadc928002/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc928002/"><img alt="Synchrotron-based high-pressure research in materials science" title="Synchrotron-based high-pressure research in materials science" src="https://digital.library.unt.edu/ark:/67531/metadc928002/thumbnail/"/></a></p><p>None</p>Kinetic Controls on the Desorption/Dissolution of Sorbed U(VI) and Their Influence on Reactive Transport2016-09-27T01:39:22-05:00https://digital.library.unt.edu/ark:/67531/metadc895000/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc895000/"><img alt="Kinetic Controls on the Desorption/Dissolution of Sorbed U(VI) and Their Influence on Reactive Transport" title="Kinetic Controls on the Desorption/Dissolution of Sorbed U(VI) and Their Influence on Reactive Transport" src="https://digital.library.unt.edu/ark:/67531/metadc895000/thumbnail/"/></a></p><p>None</p>Development of Modeling Methods and Tools for Predicting Coupled Reactive Transport Processes in Porous Media at Multiple Scales2016-09-27T01:39:22-05:00https://digital.library.unt.edu/ark:/67531/metadc895175/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc895175/"><img alt="Development of Modeling Methods and Tools for Predicting Coupled Reactive Transport Processes in Porous Media at Multiple Scales" title="Development of Modeling Methods and Tools for Predicting Coupled Reactive Transport Processes in Porous Media at Multiple Scales" src="https://digital.library.unt.edu/ark:/67531/metadc895175/thumbnail/"/></a></p><p>None</p>Mechanistically-Based Field-Scale Models of Uranium Biogeochemistry from Upscaling Pore-Scale Experiments and Models2016-09-27T01:39:22-05:00https://digital.library.unt.edu/ark:/67531/metadc894341/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc894341/"><img alt="Mechanistically-Based Field-Scale Models of Uranium Biogeochemistry from Upscaling Pore-Scale Experiments and Models" title="Mechanistically-Based Field-Scale Models of Uranium Biogeochemistry from Upscaling Pore-Scale Experiments and Models" src="https://digital.library.unt.edu/ark:/67531/metadc894341/thumbnail/"/></a></p><p>Effective environmental management of DOE sites requires reliable prediction of reactive transport phenomena. A central issue in prediction of subsurface reactive transport is the impact of multiscale physical, chemical, and biological heterogeneity. Heterogeneity manifests itself through incomplete mixing of reactants at scales below those at which concentrations are explicitly defined (i.e., the numerical grid scale). This results in a mismatch between simulated reaction processes (formulated in terms of average concentrations) and actual processes (controlled by local concentrations). At the field scale, this results in apparent scale-dependence of model parameters and inability to utilize laboratory parameters in field models. Accordingly, most field modeling efforts are restricted to empirical estimation of model parameters by fitting to field observations, which renders extrapolation of model predictions beyond fitted conditions unreliable. The objective of this project is to develop a theoretical and computational framework for (1) connecting models of coupled reactive transport from pore-scale processes to field-scale bioremediation through a hierarchy of models that maintain crucial information from the smaller scales at the larger scales; and (2) quantifying the uncertainty that is introduced by both the upscaling process and uncertainty in physical parameters. One of the challenges of addressing scale-dependent effects of coupled processes in heterogeneous porous media is the problem-specificity of solutions. Much effort has been aimed at developing generalized scaling laws or theories, but these require restrictive assumptions that render them ineffective in many real problems. We propose instead an approach that applies physical and numerical experiments at small scales (specifically the pore scale) to a selected model system in order to identify the scaling approach appropriate to that type of problem. Although the results of such studies will generally not be applicable to other broad classes of problems, we believe that this approach (if applied over time to many types of problems) offers greater potential for long-term progress than attempts to discover a universal solution or theory. We are developing and testing this approach using porous media and model reaction systems that can be both experimentally measured and quantitatively simulated at the pore scale, specifically biofilm development and metal reduction in granular porous media. The general approach we are using in this research follows the following steps: (1) Perform pore-scale characterization of pore geometry and biofilm development in selected porous media systems. (2) Simulate selected reactive transport processes at the pore scale in experimentally measured pore geometries. (3) Validate pore-scale models against laboratory-scale experiments. (4) Perform upscaling to derive continuum-scale (local darcy scale) process descriptions and effective parameters. (5) Use upscaled models and parameters to simulate reactive transport at the continuum scale in a macroscopically heterogeneous medium.</p>Impacts of Climate Variability on the California Current Ecosystem and Pacific Salmon Survival: Linkages, Ocean Condition Indicators, Forecasting, and Management Perspectives2016-09-27T01:39:22-05:00https://digital.library.unt.edu/ark:/67531/metadc894392/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc894392/"><img alt="Impacts of Climate Variability on the California Current Ecosystem and Pacific Salmon Survival: Linkages, Ocean Condition Indicators, Forecasting, and Management Perspectives" title="Impacts of Climate Variability on the California Current Ecosystem and Pacific Salmon Survival: Linkages, Ocean Condition Indicators, Forecasting, and Management Perspectives" src="https://digital.library.unt.edu/ark:/67531/metadc894392/thumbnail/"/></a></p><p>None</p>Applying EMSL Capabilities to Biogeochemistry and Environmental Research2016-09-27T01:39:22-05:00https://digital.library.unt.edu/ark:/67531/metadc895310/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc895310/"><img alt="Applying EMSL Capabilities to Biogeochemistry and Environmental Research" title="Applying EMSL Capabilities to Biogeochemistry and Environmental Research" src="https://digital.library.unt.edu/ark:/67531/metadc895310/thumbnail/"/></a></p><p>The Environmental Molecular Sciences laboratory (EMSL) is a national scientific user facility operated by the Pacific Northwest National Laboratory (PNNL) for the U.S. Department of Energy's Office of Biological and Environmental Research. Located in Richland, Washington, EMSL offers researchers a comprehensive array of cutting-edge capabilities unmatched anywhere else in the world and access to the expertise of over 300 resident users--all at one location. EMSL's resources are available on a peer-reviewed proposal basis and are offered at no cost if research results are shared in the open literature. Researchers are encouraged to submit a proposal centered around one of EMSL's four Science Themes, which represent growing areas of research: (1) Geochemistry/Biogeochemistry and Subsurface Science; (2) Atmospheric Aerosol Chemistry; (3) Biological Interactions and Dynamics; and (4) Science of Interfacial Phenomena. To learn more about EMSL, visit www.emsl.pnl.gov.</p>Photoconductive Decay Lifetime and Suns-Voc Diagnostics of Efficient Heterojunction Solar Cells2016-09-27T01:39:22-05:00https://digital.library.unt.edu/ark:/67531/metadc898331/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc898331/"><img alt="Photoconductive Decay Lifetime and Suns-Voc Diagnostics of Efficient Heterojunction Solar Cells" title="Photoconductive Decay Lifetime and Suns-Voc Diagnostics of Efficient Heterojunction Solar Cells" src="https://digital.library.unt.edu/ark:/67531/metadc898331/thumbnail/"/></a></p><p>None</p>Synthesis of Goethite-Coated Sand and Analysis of its Interactions with Uranium2016-09-27T01:39:22-05:00https://digital.library.unt.edu/ark:/67531/metadc901673/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc901673/"><img alt="Synthesis of Goethite-Coated Sand and Analysis of its Interactions with Uranium" title="Synthesis of Goethite-Coated Sand and Analysis of its Interactions with Uranium" src="https://digital.library.unt.edu/ark:/67531/metadc901673/thumbnail/"/></a></p><p>None</p>Comparison of Predictive Models for PV Module Performance (Presentation)2016-09-27T01:39:22-05:00https://digital.library.unt.edu/ark:/67531/metadc901380/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc901380/"><img alt="Comparison of Predictive Models for PV Module Performance (Presentation)" title="Comparison of Predictive Models for PV Module Performance (Presentation)" src="https://digital.library.unt.edu/ark:/67531/metadc901380/thumbnail/"/></a></p><p>This paper examines three models used to estimate the maximum power (P{sub m}) of PV modules when the irradiance and PV cell temperature are known: (1) the power temperature coefficient model, (2) the PVFORM model, and (3) the bilinear interpolation model. A variation of the power temperature coefficient model is also presented that improved model accuracy. For modeling values of P{sub m}, an 'effective' plane-of-array (POA) irradiance (E{sub e}) and the PV cell temperature (T) are used as model inputs. Using E{sub e} essentially removes the effects of variations in solar spectrum and reflectance losses, and permits the influence of irradiance and temperature on model performance for P{sub m} to be more easily studied. Eq. 1 is used to determine E{sub e} from T and the PV module's measured short-circuit current (I{sub sc}). Zero subscripts denote performance at Standard Reporting Conditions (SRC).</p>Changes in Microbial Community Structure During Biostimulation for Uranium Reduction at Different Levels of Resolution2016-09-27T01:39:22-05:00https://digital.library.unt.edu/ark:/67531/metadc893705/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc893705/"><img alt="Changes in Microbial Community Structure During Biostimulation for Uranium Reduction at Different Levels of Resolution" title="Changes in Microbial Community Structure During Biostimulation for Uranium Reduction at Different Levels of Resolution" src="https://digital.library.unt.edu/ark:/67531/metadc893705/thumbnail/"/></a></p><p>This poster describes the Changes in Microbial Community Structure During Biostimulation for Uranium Reduction at Different Levels of Resolution</p>Coupled Processes Influencing the Transport of Uranium over Multiple Scales2016-09-27T01:39:22-05:00https://digital.library.unt.edu/ark:/67531/metadc893101/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc893101/"><img alt="Coupled Processes Influencing the Transport of Uranium over Multiple Scales" title="Coupled Processes Influencing the Transport of Uranium over Multiple Scales" src="https://digital.library.unt.edu/ark:/67531/metadc893101/thumbnail/"/></a></p><p>None</p>Influence of Wetting and Mass Transfer Properties of Organic Chemical Mixtures in Vadose Zone Materials on Groundwater Contamination by Nonaqueous Phase Liquids2016-09-27T01:39:22-05:00https://digital.library.unt.edu/ark:/67531/metadc895933/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc895933/"><img alt="Influence of Wetting and Mass Transfer Properties of Organic Chemical Mixtures in Vadose Zone Materials on Groundwater Contamination by Nonaqueous Phase Liquids" title="Influence of Wetting and Mass Transfer Properties of Organic Chemical Mixtures in Vadose Zone Materials on Groundwater Contamination by Nonaqueous Phase Liquids" src="https://digital.library.unt.edu/ark:/67531/metadc895933/thumbnail/"/></a></p><p>None</p>Biogeochemical Mechanisms Controlling Reduced Radionuclide Particle Properties and Stability2016-09-27T01:39:22-05:00https://digital.library.unt.edu/ark:/67531/metadc893991/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc893991/"><img alt="Biogeochemical Mechanisms Controlling Reduced Radionuclide Particle Properties and Stability" title="Biogeochemical Mechanisms Controlling Reduced Radionuclide Particle Properties and Stability" src="https://digital.library.unt.edu/ark:/67531/metadc893991/thumbnail/"/></a></p><p>None</p>The Center for Environmental Kinetics Analysis: an NSF- and DOE-funded Environmental Molecular Science Institute (EMSI) at Penn State2016-09-27T01:39:22-05:00https://digital.library.unt.edu/ark:/67531/metadc897869/<p><a href="https://digital.library.unt.edu/ark:/67531/metadc897869/"><img alt="The Center for Environmental Kinetics Analysis: an NSF- and DOE-funded Environmental Molecular Science Institute (EMSI) at Penn State" title="The Center for Environmental Kinetics Analysis: an NSF- and DOE-funded Environmental Molecular Science Institute (EMSI) at Penn State" src="https://digital.library.unt.edu/ark:/67531/metadc897869/thumbnail/"/></a></p><p>Physicochemical and microbiological processes taking place at environmental interfaces influence natural processes as well as the transport and fate of environmental contaminants, the remediation of toxic chemicals, and the sequestration of anthropogenic CO2. A team of scientists and engineers has been assembled to develop and apply new experimental and computational techniques to expand our knowledge of environmental kinetics. We are also training a cohort of talented and diverse students to work on these complex problems at multiple length scales and to compile and synthesize the kinetic data. Development of the human resources capable of translating molecular-scale information into parameters that are applicable in real world, field-scale problems of environmental kinetics is a major and relatively unique objective of the Institute's efforts. The EMSI team is a partnership among 10 faculty at The Pennsylvania State University (funded by the National Science Foundation Divisions of Chemistry and Earth Sciences), one faculty member at Juniata College, one faculty member at the University of Florida, and four researchers drawn from Los Alamos National Laboratory, Pacific Northwest National Laboratory, and Lawrence Berkeley National Laboratory (funded by the Department of Energy Division of Environmental Remediation Sciences). Interactions among the applied and academic scientists drives research approaches aimed toward solving important problems of national interest. The Institute is organized into three interest groups (IGs) focusing on the processes of dissolution (DIG), precipitation (PIG), and microbial reactions at surfaces (BIG). Some of the research activity from each IG is highlighted to the right. The IGs interact with each other as each interest group studies reactions across the molecular, microscopic, mesoscopic and, in most cases, field scales. For example, abiotic dissolution and precipitation reactions of Fe oxides as studied in the Dissolution IG provides the baseline for kinetic behavior as the BIG researches the interaction of microorganisms with these same minerals. The attachment of bacteria and redox chemistry that occurs between microorganisms and minerals are critical factors in maintaining groundwater quality and remediation of many toxic waste sites and is one of the main thrusts of research within our EMSI. The IGs also participate in using visualization tools to promote greater understanding of complex environmental data. As a whole, CEKA is also working to compile environmental kinetics data into a cyberinfrastructure and database. The database can be accessed at: http://keystone.ist.psu.edu/.</p>