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How will the United States satisfy energy demand in a tightening global energy marketplace while, at the same time, reducing greenhouse gas emissions? Exascale computing -- expected to be available within the next eight to ten years ? may play a crucial role in answering that question by enabling a paradigm shift from test-based to science-based design and engineering. Computational modeling of complete power generation systems and engines, based on scientific first principles, will accelerate the improvement of existing energy technologies and the development of new transformational technologies by pre-selecting the designs most likely to be successful for experimental validation, rather than relying on trial and error. The predictive understanding of complex engineered systems made possible by computational modeling will also reduce the construction and operations costs, optimize performance, and improve safety. Exascale computing will make possible fundamentally new approaches to quantifying the uncertainty of safety and performance engineering. This report discusses potential contributions of exa-scale modeling in four areas of energy production and distribution: nuclear power, combustion, the electrical grid, and renewable sources of energy, which include hydrogen fuel, bioenergy conversion, photovoltaic solar energy, and wind turbines. Examples of current research are taken from projects funded by the U.S. …

Chemolithoautotrophic bacteria can be of industrial and environmental importance, but they present a challenge for systems biology studies, as their central metabolism deviates from that of model organisms and there is a much less extensive experimental basis for their gene annotation than for typical organoheterotrophs. For microbes with sequenced genomes but unconventional metabolism, the ability to create knockout mutations can be a powerful tool for functional genomics and thereby render an organism more amenable to systems biology approaches. In this chapter, we describe a genetic system for Thiobacillus denitrificans, with which insertion mutations can be introduced by homologous recombination and complemented in trans. Insertion mutations are generated by in vitro transposition, the mutated genes are amplified by the PCR, and the amplicons are introduced into T. denitrificans by electroporation. Use of a complementation vector, pTL2, based on the IncP plasmid pRR10 is also addressed.

This report discusses the continuing conflict in Syria including recent regime losses in confrontations with forces of the Islamic State (IS, aka the Islamic State of Iraq and the Levant or ISIL/ISIS) in northeastern Syria. The report also covers U.S. policy responses to the conflict.

This report responds to a series of frequently asked questions (FAQs) identified to address some of the major updates included in the proposed rule. The FAQs summarize provisions such as the introduction of a minimum medical loss ratio (MLR), guidance on enrolling the long-term services and supports (LTSS) population in managed care, and network adequacy.

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Organometallic chemistry has played and will continue to play a significant role in helping us understand the way bonds are made or broken in the presence of a transition metal complex. Current challenges range from the efficient exploitation of energy resources to the creative use of natural and artificial enzymes. Most of the new advances in the area are due to our extended understanding of processes at a molecular level due to new mechanistic studies, techniques to detect reaction intermediates and theory. The conference will bring the most recent advances in the field including nanocatalysis, surface organometallic chemistry, characterization techniques, new chemical reactivity and theoretical approaches along with applications to organic synthesis and the discovery of new materials. The Conference will bring together a collection of investigators who are at the forefront of their field, and will provide opportunities for junior scientists and graduate students to present their work in poster format and exchange ideas with leaders in the field. Six outstanding posters will be selected for short talks. The collegial atmosphere of this Conference, with programmed discussion sessions as well as opportunities for informal gatherings in the afternoons and evenings, provides an avenue for scientists from different disciplines to …

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Stimulated by an acetate-amendment field experiment conducted in 2007, anaerobic microbial populations in the aquifer at the Rifle Integrated Field Research Challenge site in Colorado reduced mobile U(VI) to insoluble U(IV). During this experiment, planktonic biomass was sampled at various time points to quantitatively evaluate proteomes. In 2008, an acetate-amended field experiment was again conducted in a similar manner to the 2007 experiment. As there was no comprehensive metagenome sequence available for use in proteomics analysis, we systematically evaluated 12 different organism genome sequences to generate sets of aggregate genomes, or “pseudo-metagenomes”, for supplying relative quantitative peptide and protein identifications. Proteomics results support previous observations of the dominance of Geobacteraceae during biostimulation using acetate as sole electron donor, and revealed a shift from an early stage of iron reduction to a late stage of iron reduction. Additionally, a shift from iron reduction to sulfate reduction was indicated by changes in the contribution of proteome information contributed by different organism genome sequences within the aggregate set. In addition, the comparison of proteome measurements made between the 2007 field experiment and 2008 field experiment revealed differences in proteome profiles. These differences may be the result of alterations in abundance and population structure …

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This report explains the process for filling positions to which the President makes appointments with the advice and consent of the Senate (also referred to as PAS positions). It also identifies, for the 111th Congress, all nominations to full-time positions requiring Senate confirmation in the 15 executive departments.

This Hazard and Operability (HAZOP) study addresses the Sludge Treatment Project (STP) Engineered Container Retrieval and Transfer System (ECRTS) preliminary design for retrieving sludge from underwater engineered containers located in the 105-K West (KW) Basin, transferring the sludge as a sludge-water slurry (hereafter referred to as 'slurry') to a Sludge Transport and Storage Container (STSC) located in a Modified KW Basin Annex, and preparing the STSC for transport to T Plant using the Sludge Transport System (STS). There are six, underwater engineered containers located in the KW Basin that, at the time of sludge retrieval, will contain an estimated volume of 5.2 m{sup 3} of KW Basin floor and pit sludge, 18.4 m{sup 3} of 105-K East (KE) Basin floor, pit, and canister sludge, and 3.5 m{sup 3} of settler tank sludge. The KE and KW Basin sludge consists of fuel corrosion products (including metallic uranium, and fission and activation products), small fuel fragments, iron and aluminum oxide, sand, dirt, operational debris, and biological debris. The settler tank sludge consists of sludge generated by the washing of KE and KW Basin fuel in the Primary Clean Machine. A detailed description of the origin of sludge and its chemical and physical …

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The ability to conduct advanced functional genomic studies of the thousands of sequenced bacteria has been hampered by the lack of available tools for making high- throughput chromosomal manipulations in a systematic manner that can be applied across diverse species. In this work, we highlight the use of synthetic biological tools to assemble custom suicide vectors with reusable and interchangeable DNA “parts” to facilitate chromosomal modification at designated loci. These constructs enable an array of downstream applications including gene replacement and creation of gene fusions with affinity purification or localization tags. We employed this approach to engineer chromosomal modifications in a bacterium that has previously proven difficult to manipulate genetically, Desulfovibrio vulgaris Hildenborough, to generate a library of over 700 strains. Furthermore, we demonstrate how these modifications can be used for examining metabolic pathways, protein-protein interactions, and protein localization. The ubiquity of suicide constructs in gene replacement throughout biology suggests that this approach can be applied to engineer a broad range of species for a diverse array of systems biological applications and is amenable to high-throughput implementation.

This report discusses the 2002 rule, focusing on how it changes which material and types of activities are regulated under Section 404 and the significance of these issues, especially for the mining industry.

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This report provides an overview of the six grant types with criteria for defining a block grant and a list of current block grants. It also examines competing perspectives concerning the use of block grants versus other grant mechanisms to achieve national goals, provides an historical overview of the role of block grants in American federalism, and discusses recent changes to existing block grants and proposals to create new ones.

John Dalton first proposed the concept of atomic weights of the elements in the first decade of the nineteenth century. These atomic weights of the chemical elements were thought of as constants of nature, similar to the speed of light. Dmitri Mendeleev arranged the atomic weights of the elements in ascending order of value and used the systematic variation of their chemical properties to produce his Periodic Table of the Elements in 1869. Measurement of atomic weight values became an important chemical activity for a century and a half. Theodore Richards received a Noble Prize for his work in this area. In 1913, Fredrick Soddy found a species of radium, which had an atomic weight value of 228, compared to the familiar radium gas value of 226. Soddy coined the term 'isotope' (Greek for 'in the same place') to account for this second atomic weight value in the radium position of the Periodic Table. Both of these isotopes of radium are radioactive. Radioactive isotopes are energetically unstable and will decay (disintegrate) over time. The time it takes for one half of a sample of a given radioactive isotope to decay is the half-life of that isotope. In addition to having …

This report has three major sections. The first section answers some basic questions about Bitcoin and the operation of the Bitcoin network and its interaction with the current dollar-based monetary system. The second section summarizes likely reasons for and against widespread Bitcoin adoption. The third section discusses legal and regulatory matters that have been raised by Bitcoin and other digital currencies.

This report discusses Special Operations Forces (SOFs), which are elite military units with special training and equipment that can infiltrate hostile territory through land, sea, or air to conduct a variety of operations, many of them classified.

This report discusses Special Operations Forces (SOFs), which are elite military units with special training and equipment that can infiltrate hostile territory through land, sea, or air to conduct a variety of operations, many of them classified.

The objectives are: (1) develop advanced materials and coatings for hydrogen pipeline compressors; (2) achieve greater reliability, greater efficiency, and lower capital in vestment and maintenance costs in hydrogen pipeline compressors; and (3) research existing and novel hydrogen compression technologies that can improve reliability, eliminate contamination, and reduce cost. Compressors are critical components used in the production and delivery of hydrogen. Current reciprocating compressors used for pipeline delivery of hydrogen are costly, are subject to excessive wear, have poor reliability, and often require the use of lubricants that can contaminate the hydrogen (used in fuel cells). Duplicate compressors may be required to assure availability. The primary objective of this project is to identify, and develop as required, advanced materials and coatings that can achieve the friction, wear, and reliability requirements for dynamically loaded components (seal and bearings) in high-temperature, high-pressure hydrogen environments prototypical of pipeline and forecourt compressor systems. The DOE Strategic Directions for Hydrogen Delivery Workshop identified critical needs in the development of advanced hydrogen compressors - notably, the need to minimize moving parts and to address wear through new designs (centrifugal, linear, guided rotor, and electrochemical) and improved compressor materials. The DOE is supporting several compressor design studies …

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