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Methods for Engineering Sulfate Reducing Bacteria of the Genus Desulfovibrio
Sulfate reducing bacteria are physiologically important given their nearly ubiquitous presence and have important applications in the areas of bioremediation and bioenergy. This chapter provides details on the steps used for homologous-recombination mediated chromosomal manipulation of Desulfovibrio vulgaris Hildenborough, a well-studied sulfate reducer. More specifically, we focus on the implementation of a 'parts' based approach for suicide vector assembly, important aspects of anaerobic culturing, choices for antibiotic selection, electroporation-based DNA transformation, as well as tools for screening and verifying genetically modified constructs. These methods, which in principle may be extended to other sulfate-reducing bacteria, are applicable for functional genomics investigations, as well as metabolic engineering manipulations.
Date:
March 15, 2011
Creator:
Chhabra, Swapnil R; Keller, Kimberly L. & Wall, Judy D.
System:
The UNT Digital Library
MPACT FY2011 Advanced Time-Correlated Measurement Research at INL
Simulations and experiments have been carried out to investigate advanced time-correlated measurement methods for characterizing and assaying nuclear material for safeguarding the nuclear fuel cycle. These activities are part of a project studying advanced instrumentation techniques in support of the U.S. Department of Energy's Fuel Cycle Research and Development program and its Materials Protection, Accounting, and Control Technologies (MPACT) program. For fiscal year 2011 work focused on examining the practical experimental aspects of using a time-tagged, associated-particle electronic neutron generator for interrogating low-enrichment uranium in combination with steady-state interrogation using a moderated 241Am-Li neutron source. Simulation work for the project involved the use of the MCNP-PoliMi Monte Carlo simulation tool to determine the relative strength and the time-of-flight energy spectra of different sample materials under irradiation. Work also took place to develop a post-processor parser code to extract comparable data from the MCNP5&6 codes. Experiments took place using a commercial deuterium-tritium associated-particle electronic neutron generator to irradiate a number of uranium-bearing material samples. Time-correlated measurements of neutron and photon signatures of these measurements were made using five liquid scintillator detectors in a novel array, using high-speed waveform digitizers for data collection. This report summarizes the experiments that took place in …
Date:
September 1, 2011
Creator:
Chichester, D. L.; Pozzi, S. A.; Dolan, J. L.; Flaska, M. & Watson, S. M.
System:
The UNT Digital Library
Cisco College Requests for Legislative Appropriations: 2018 and 2019
Report submitted by Cisco College to the Texas 85th regular legislature requesting appropriations to fund programming and activities. It includes an overview of the institution's goals, summaries of appropriations requests for fiscal years 2018 and 2019, and supporting documentation.
Date:
August 3, 2016
Creator:
Cisco College
System:
The Portal to Texas History
Eagle Mountain Watershed Management
This document provides information on watershed management for Eagle Mountain Lake.
Date:
February 2012
Creator:
Clayton, Brent
System:
The Portal to Texas History
Red Bluff Water Treatment Plant Project Annual Budget: 2015
Proposed budget for the Coastal Water Authority Red Bluff Water Treatment Plant in Harris County, Texas outlining projected income and expenditures, with supporting documentation.
Date:
November 12, 2014
Creator:
Coastal Water Authority
System:
The Portal to Texas History
Trinity River Water Conveyance Project Annual Budget: 2014
Proposed budget for the Trinity River Water Conveyance Project outlining projected income and expenditures, with supporting documentation, during fiscal year 2014.
Date:
Autumn 2013
Creator:
Coastal Water Authority
System:
The Portal to Texas History
Trinity River Water Conveyance Project Annual Budget: 2015
Proposed budget for the Trinity River Water Conveyance Project outlining projected income and expenditures, with supporting documentation, during fiscal year 2015.
Date:
2015
Creator:
Coastal Water Authority
System:
The Portal to Texas History
Bayport Water System Project Annual Budget: 2014
Annual budget for the Coastal Water Authority Bayport Water System Project including schedules of revenues and expenditures, with supporting documentation for fiscal year 2014. It includes the text of resolutions adopting the budget.
Date:
Autumn 2013
Creator:
Coastal Water Authority (Tex.)
System:
The Portal to Texas History
Bayport Water System Project Annual Budget: 2017
Annual budget for the Coastal Water Authority Bayport Water System Project including schedules of revenues and expenditures, with supporting documentation for fiscal year 2017.
Date:
2016
Creator:
Coastal Water Authority (Tex.)
System:
The Portal to Texas History
Lake Houston Facilities Project Annual Budget: 2012
Proposed budget for the Coastal Water Authority Lake Houston Facilities Project outlining projected income and expenditures, with supporting documentation.
Date:
November 9, 2011
Creator:
Coastal Water Authority (Tex.)
System:
The Portal to Texas History
Lake Houston Facilities Project Annual Budget: 2013
Proposed budget for the Coastal Water Authority Lake Houston Facilities Project outlining projected income and expenditures, with supporting documentation.
Date:
2012
Creator:
Coastal Water Authority (Tex.)
System:
The Portal to Texas History
Lake Houston Facilities Project Annual Budget: 2014
Proposed budget for the Coastal Water Authority Lake Houston Facilities Project outlining projected income and expenditures, with supporting documentation.
Date:
Autumn 2013
Creator:
Coastal Water Authority (Tex.)
System:
The Portal to Texas History
Lake Houston Facilities Project Annual Budget: 2015
Proposed budget for the Coastal Water Authority Lake Houston Facilities Project outlining projected income and expenditures, with supporting documentation.
Date:
2015
Creator:
Coastal Water Authority (Tex.)
System:
The Portal to Texas History
Lake Houston Facilities Project Annual Budget: 2017
Proposed budget for the Coastal Water Authority Lake Houston Facilities Project outlining projected income and expenditures, with supporting documentation.
Date:
2017
Creator:
Coastal Water Authority (Tex.)
System:
The Portal to Texas History
Red Bluff Water Treatment Plant Project Annual Budget: 2014
Proposed budget for the Coastal Water Authority Red Bluff Water Treatment Plant in Harris County, Texas outlining projected income and expenditures, with supporting documentation.
Date:
2013
Creator:
Coastal Water Authority (Tex.)
System:
The Portal to Texas History
Red Bluff Water Treatment Plant Project Annual Budget: 2017
Proposed budget for the Coastal Water Authority Red Bluff Water Treatment Plant in Harris County, Texas outlining projected income and expenditures, with supporting documentation.
Date:
2016
Creator:
Coastal Water Authority (Tex.)
System:
The Portal to Texas History
Trinity River Water Conveyance Project Annual Budget: 2017
Proposed budget for the Trinity River Water Conveyance Project outlining projected income and expenditures, with supporting documentation, during fiscal year 2017.
Date:
2017
Creator:
Coastal Water Authority (Tex.)
System:
The Portal to Texas History
Wastebook 2012
Senator Tom Coburn released his Wastebook 2012, a report outlining wasteful federal policies that cost taxpayers more than $19 billion dollars a year.
Date:
October 2012
Creator:
Coburn, Tom
System:
The UNT Digital Library
Characterization of Geomorphic Units in Alluvial Valleys and Channels of Gulf Coastal Plain Rivers in Texas, with Examples from the Brazos, Sabine, and Trinity Rivers, 2010
This document provides information on the characterization of geomorphi units in the Alluvial Valleys and Channels of Gulf Coastal Plain Rivers in Texas.
Date:
2010
Creator:
Coffman, David K.; Malstaff, Greg & Heitmuller, Franklin T.
System:
The Portal to Texas History
Collin County Community College District Requests for Legislative Appropriations: Fiscal Years 2014 and 2015
Report submitted by the Collin County Community College District containing summaries of requests for appropriations and expenditures for the fiscal years 2014 and 2015 with supporting documentation.
Date:
August 16, 2012
Creator:
Collin County Community College District
System:
The Portal to Texas History
Comptroller Susan Combs Distributes $433 Million in Monthly Sales Tax Revenue to Local Governments
This document provides information on the distribution of $433 million in monthly sales tax revenue to local government.
Date:
March 9, 2011
Creator:
Combs, Susan
System:
The Portal to Texas History
Comptroller Susan Combs Distributes $468 Million in Monthly Sales Tax Revenue to Local Governments
This document provides information on the distribution of $468 million in monthly sales tax revenue to local government.
Date:
June 8, 2011
Creator:
Combs, Susan
System:
The Portal to Texas History
Comptroller Susan Combs Distributes $483 Million in Monthly Sales Tax Revenue to Local Governments
This document provides information on the distribution of $483 million in monthly sales tax revenue to local government.
Date:
September 8, 2011
Creator:
Combs, Susan
System:
The Portal to Texas History
Comptroller Susan Combs Distributes $489 Million in Monthly Sales Tax Revenue to Local Governments
This document provides information on the distributions of $489 million in monthly sales tax revenue to local governments.
Date:
April 11, 2012
Creator:
Combs, Susan
System:
The Portal to Texas History