Human genome sequencing with direct x-ray holographic imaging (open access)

Human genome sequencing with direct x-ray holographic imaging

Direct holographic imaging of biological materials is widely applicable to the study of the structure, properties and action of genetic material. This particular application involves the sequencing of the human genome where prospective genomic imaging technology is composed of three subtechnologies, name an x-ray holographic camera, suitable chemistry and enzymology for the preparation of tagged DNA samples, and the illuminator in the form of an x-ray laser. We report appropriate x-ray camera, embodied by the instrument developed by MCR, is available and that suitable chemical and enzymatic procedures exist for the preparation of the necessary tagged DNA strands. Concerning the future development of the x-ray illuminator. We find that a practical small scale x-ray light source is indeed feasible. This outcome requires the use of unconventional physical processes in order to achieve the necessary power-compression in the amplifying medium. The understanding of these new physical mechanisms is developing rapidly. Importantly, although the x-ray source does not currently exist, the understanding of these new physical mechanisms is developing rapidly and the research has established the basic scaling laws that will determine the properties of the x-ray illuminator. When this x-ray source becomes available, an extremely rapid and cost effective instrument for …
Date: June 8, 1993
Creator: Rhodes, C.K.
Object Type: Report
System: The UNT Digital Library
Texas Register, Volume 18, Number 44, Pages 3589-3632, June 8, 1993 (open access)

Texas Register, Volume 18, Number 44, Pages 3589-3632, June 8, 1993

A weekly publication, the Texas Register serves as the journal of state agency rulemaking for Texas. Information published in the Texas Register includes proposed, adopted, withdrawn and emergency rule actions, notices of state agency review of agency rules, governor's appointments, attorney general opinions, and miscellaneous documents such as requests for proposals. After adoption, these rulemaking actions are codified into the Texas Administrative Code.
Date: June 8, 1993
Creator: Texas. Secretary of State.
Object Type: Journal/Magazine/Newsletter
System: The Portal to Texas History
Texas Attorney General Opinion: DM-225 (open access)

Texas Attorney General Opinion: DM-225

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Dan Morales, regarding a legal question submitted for clarification; Liability for costs of health care provided to indigent inmates of the Karnes County Jail (RQ-420)
Date: June 8, 1993
Creator: Texas. Attorney-General's Office.
Object Type: Text
System: The Portal to Texas History
Texas Attorney General Opinion: LO93-046 (open access)

Texas Attorney General Opinion: LO93-046

Letter opinion issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Dan Morales, regarding a legal question submitted for clarification: Whether article VII, section 9 of the Texas Constitution grants the Parrie Haynes Ranch, which was willed “to the State Orphan Home of Texas to help orphan children,” to the asylum fund established by the constitutional provision (RQ-432)
Date: June 8, 1993
Creator: Texas. Attorney-General's Office.
Object Type: Text
System: The Portal to Texas History
Human genome sequencing with direct x-ray holographic imaging. Final report (open access)

Human genome sequencing with direct x-ray holographic imaging. Final report

Direct holographic imaging of biological materials is widely applicable to the study of the structure, properties and action of genetic material. This particular application involves the sequencing of the human genome where prospective genomic imaging technology is composed of three subtechnologies, name an x-ray holographic camera, suitable chemistry and enzymology for the preparation of tagged DNA samples, and the illuminator in the form of an x-ray laser. We report appropriate x-ray camera, embodied by the instrument developed by MCR, is available and that suitable chemical and enzymatic procedures exist for the preparation of the necessary tagged DNA strands. Concerning the future development of the x-ray illuminator. We find that a practical small scale x-ray light source is indeed feasible. This outcome requires the use of unconventional physical processes in order to achieve the necessary power-compression in the amplifying medium. The understanding of these new physical mechanisms is developing rapidly. Importantly, although the x-ray source does not currently exist, the understanding of these new physical mechanisms is developing rapidly and the research has established the basic scaling laws that will determine the properties of the x-ray illuminator. When this x-ray source becomes available, an extremely rapid and cost effective instrument for …
Date: June 8, 1993
Creator: Rhodes, C. K.
Object Type: Report
System: The UNT Digital Library
Investigation of the relative abundance of heavy versus light nuclei in primary cosmic rays using underground muon bundles (open access)

Investigation of the relative abundance of heavy versus light nuclei in primary cosmic rays using underground muon bundles

We study multiple muon events (muon bundles) recorded underground at a depth of 2090 mwe. To penetrate to this depth, the muons must have energies above 0.8 TeV at the Earth`s surface; the primary cosmic ray nuclei which give rise to the observed muon bundles have energies at incidence upon the upper atmosphere of 10 to 10{sup 5}TeV. The events are detected using the Soudan 2 experiment`s fine grained tracking calorimeter which is surrounded by a 14 m {times}10 m {times} 31 m proportional tube array (the ``active shield``). Muon bundles which have at least one muon traversing the calorimeter, are reconstructed using tracks in the calorimeter together with hit patterns in the proportional tube shield. All ionization pulses are required to be coincident within 3 microseconds. A goal of this study is to investigate the relative nuclear abundances in the primary cosmic radiation around the ``knee`` region (10{sup 3} {minus} 10{sup 4} TeV) of the incident energy spectrum. Four models for the nuclear composition of cosmic rays are considered: The Linsley model, the Constant Mass Composition model (CMC), the Maryland model and the Proton-poor model. A Monte Carlo which incorporates one model at a time is used to simulate …
Date: June 8, 1993
Creator: Sundaralingam, N.
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Transmission Electron Microscopy Study of Thick Copper-304 Stainless Steel Multilayers (open access)

Transmission Electron Microscopy Study of Thick Copper-304 Stainless Steel Multilayers

Thick (10 to 25 {mu}m), free-standing, equal layer thickness, Copper(Cu)-304 Stainless Steel(SS) multilayer foils, having periods of 1 to 100 nm, synthesized by magnetron sputter deposition, were examined by plan view and cross-sectional transmission electron microscopy. Multilayer growth morphology, individual layer structure and crystallographic phase orientation relationships were characterized in this study. Electron Energy Loss filtered imaging of a 20 nm period multilayer cross-section was also performed and showed that nickel had diffused into the Cu layers from the SS during synthesis. X-ray powder diffraction scans were performed and analyzed. A pure deposit of 304SS was synthesized and had a metastable BCC structure. Multilayer samples having periods of 20 nm were found to have a coherent layered Cu(FCC)SS(FCC) structure. At larger periods (50 & 100 nm) a bimodal Cu(FCC)-SS(FCC & BCC) structure was formed. These observations show that the 304SS will grow with a metastable BCC structure when sputter deposited. When layered with Cu(FCC) the 304SS has its equilibrium FCC structure at layer thicknesses up to 10nm as a result of epitaxy with the copper. At larger SS layer thicknesses the SS appears to locally transform to the metastable BCC structure during synthesis, refining the grain structure of the depositing …
Date: June 8, 1993
Creator: Wall, M. A.; Barbee, T. W., Jr. & Weihs, T.
Object Type: Article
System: The UNT Digital Library