A letter report issued by the Government Accountability Office with an abstract that begins "The financing of the federal government depends largely on the Internal Revenue Service's (IRS) ability to administer the tax laws, which includes providing service to taxpayers and enforcing the law so that individuals and businesses pay the taxes they owe. The President's fiscal year 2010 budget request details how IRS intends to allocate its resources to achieve these goals while also continuing its investment modernizing its tax processing systems. GAO was asked to (1) describe budget trends, including dollars and full time equivalents; (2) describe legislative proposals that, if enacted, could result in savings or increased revenues; (3) describe the requested increases in funding for new initiatives; (4) assess return on investment (ROI) information; and (5) assess and the status of the Business Systems Modernization (BSM) program. To do this, GAO compared the budget request to prior years, analyzed key documents, and interviewed IRS officials"

A letter report issued by the Government Accountability Office with an abstract that begins "Since 2003, the United States has provided $49 billion to help rebuild Iraq. To build the capacity of Iraq's central and provincial governments to sustain this effort, the United States is implementing programs including Department of State's (State) Provincial Reconstruction Development Committee (PRDC) and the U.S. Agency for International Development's (USAID) National Capacity Development (NCD). The use of key management controls, such as appropriate organizational structure and program monitoring, helps ensure programs achieve their objectives. Through field visits in Iraq, interviews with program officials, analyses of official reports, and examination of a sample of projects, we assessed whether the PRDC and NCD's management controls support the programs' objectives of building the capacity of Iraq's government. We also assessed Iraq's commitment to sustaining these U.S. programs."

A letter report issued by the Government Accountability Office with an abstract that begins "In December 2003, GAO reported that most of Alaska's more than 200 Native villages were affected to some degree by flooding and erosion (GAO-04-142). Since 2003, state officials have identified the growing impacts of climate change, increasing the urgency of federal and state efforts to identify imminently threatened villages and assess their relocation options. GAO was asked to report on (1) the flooding and erosion threats that Alaska Native villages currently face, (2) the federal programs that are available to assist villages facing potential disasters, (3) the status of village relocation efforts, and (4) how federal assistance to relocating villages is prioritized. GAO interviewed and gathered documentation from federal and state agency officials as well as regional organizations and village representatives."

Testimony issued by the Government Accountability Office with an abstract that begins "As the recent outbreak of the H1N1 (swine flu) virus underscores, an influenza pandemic remains a real threat to our nation and to the world. Over the past 3 years, GAO has conducted a body of work to help the nation better prepare for a possible pandemic. In a February 2009 report, GAO synthesized the results of this work, pointing out that while the previous administration had taken a number of actions to plan for a pandemic, including developing a national strategy and implementation plan, much more needs to be done, and many gaps in preparedness and planning still remain. This statement is based on the February 2009 report which synthesized the results of 11 reports and two testimonies covering six thematic areas: (1) leadership, authority, and coordination; (2) detecting threats and managing risks; (3) planning, training, and exercising, (4) capacity to respond and recover; (5) information sharing and communication; and (6) performance and accountability."

Testimony issued by the Government Accountability Office with an abstract that begins "The New Starts program is an important source of new capital investment in mass transportation. As required by the Safe, Accountable, Flexible, Efficient Transportation Equity Act: A Legacy for Users, the Federal Transit Administration (FTA) must prioritize transit projects for funding by evaluating, rating, and recommending projects on the basis of specific financial commitment and project justification criteria, such as cost-effectiveness, economic development effects, land use, and environmental benefits. To be eligible for federal funding, a project must advance through the different project development phases of the New Starts program, including alternatives analysis, preliminary engineering, and final design. Using the statutorily identified criteria, FTA evaluates projects as a condition for advancement into each project development phase of the program. This testimony discusses the (1) key challenges associated with the New Starts program and (2) options that could help expedite project development in the New Starts program. This testimony is based on GAO's extensive body of work on the New Starts program and ongoing work--as directed by Congress. For this work, GAO reviewed FTA documents and interviewed FTA officials, sponsors of New Starts projects, and representatives from industry associations. …

A letter report issued by the Government Accountability Office with an abstract that begins "Every tax filing season, the Internal Revenue Service (IRS) must quickly and accurately process Americans' tax returns, issue refunds, and answer taxpayers' questions. The 2009 filing season, when 2008 tax returns are filed, was especially challenging because of stimulus-related tax law changes and IRS's expanded assistance to financially distressed taxpayers, such as adding flexibility for taxpayers to settle a tax liability for less than the full amount owed. Because of the magnitude and importance of the 2009 filing season, GAO was asked to (1) assess IRS's performance in processing individual income tax returns and providing taxpayer assistance, (2) describe taxpayer use of refund anticipation loans (RAL) and refund anticipation checks (RAC), and (3) identify what IRS knows about the number of taxpayers using its expanded assistance to financially distressed taxpayers. GAO analyzed performance data from IRS, compared the performance to prior years and selected goals, and interviewed IRS officials and external stakeholders."

This report discusses the relationship between the United States and Japan, including Japanese efforts to support the U.S. in the wake of the September 11th, 2001 terrorist attacks and the two countries' economic ties, especially with regards to the recent economic downturn. This report also discusses the general political atmosphere of Japan, including the recent and ongoing political turmoil in Tokyo.

This report analyzes Brazil's political, economic, and social conditions, and how those conditions affect its role in the region and its relationship with the United States.

This report discusses the U.S.-China economic relationship and China's rapid expansion as a global economic market, both with respect to the current global economic crisis. It also examines major U.S.-China trade issues and related legislation.

Section 1207 of the National Defense Authorization Act (NDAA) for Fiscal Year 2006 provides authority for the Department of Defense (DOD) to transfer to the State Department up to $100 million per fiscal year in defense articles, services, training or other support for reconstruction, stabilization, and security activities in foreign countries. The 111th Congress must decide whether to extend Section 1207, which expires on September 30, 2009. This report discusses what issues Congress must take into consideration when making this decision.

This report discusses the economy of China and how it is has been affected by the recent economic downturn. China has recently enjoyed one of the world's fastest-growing economies and has been a major contributor to world economic growth, but several Chinese industries have been hard by the crisis, and millions of workers have been laid off. This report explores this issue in brief, including what actions the Chinese government is taking to combat the problem, as well as what actions China may take to assist in stabilizing the U.S. economy.

This report discusses the state of the Kurdish-inhabited region of northern Iraq, which has been fairly peaceful since the fall of Saddam Hussein; however, the region is also home to friction with Christian and other minorities, with Prime Minister Nuri al-Maliki and other Arab leaders of Iraq, and with neighboring Turkey and Iran. The report also addresses other general political issues and tensions in the Kurdish region, and how said tensions might affect and be affected by the projected U.S. drawdown of U.S. forces in Iraq between now and August 2010.

The 111th Congress in coming months might take up free trade agreements (FTAs) signed by the Bush Administration with Colombia, Panama, and South Korea under trade promotion authority, or fast-track rules, designed to expedite congressional consideration of these agreements. Accordingly, agriculture as covered in each pending trade agreement is examined in this report in the order that Congress likely will take up these agreements, based upon statements made to date by Obama Administration officials and Members of Congress.

According to Bechtel National Inc.’s (BNI’s) Test Specification 24590-PTF-TSP-RT-06-006, Rev 0, “Simulant Development to Support the Development and Demonstration of Leaching and Ultrafiltration Pretreatment Processes,” simulants for boehmite, gibbsite, and filtration are to be developed that can be used in subsequent bench and integrated testing of the leaching/filtration processes. These simulants will then be used to demonstrate the leaching process and to help refine processing conditions that may impact safety basis considerations (Smith 2006). This report documents the results of the boehmite simulant development and blended simulant crossflow ultrafiltration leaching completed in accordance with the test plan TP-RPP-WTP-469 Rev 0 (WTP Doc. No. 24590- 101-TSA-W000-0004-182-00001 Rev 00A) prepared and approved in response to the cited test specification. This report also includes the results of the aluminate and anion effect on boehmite dissolution performed in accordance with the test plan TP-RPP-WTP-509, Rev 0 (WTP Doc. No. 24590-101-TSA-W000-0004-72-00019 Rev 00A) prepared and approved in response to the Test Specification 24590-WTP-TSP-RT-07-004, Rev 0 (Sundar 2007).

We have shown that branching morphogenesis of mammary ductal structures requires the action of the morphogen epimorphin/syntaxin-2. Epimorphin, originally identified as an extracellular molecule, is identical to syntaxin-2, an intracellular molecule that is a member of the extensively investigated syntaxin family of proteins that mediate vesicle trafficking. We show here that although epimorphin/syntaxin-2 is highly homologous to syntaxin-1a, only epimorphin/syntaxin-2 can stimulate mammary branching morphogenesis. We construct a homology model of epimorphin/syntaxin-2 based on the published structure of syntaxin-1a, and we use this model to identify the structural motif responsible for the morphogenic activity. We identify four residues located within the cleft between helices B and C that differ between syntaxin-1a and epimorphin/syntaxin-2; through site-directed mutagenesis of these four amino acids, we confer the properties of epimorphin for cell adhesion, gene activation, and branching morphogenesis onto the inactive syntaxin-1a template. These results provide a dramatic demonstration of the use of structural information about one molecule to define a functional motif of a second molecule that is related at the sequence level but highly divergent functionally.

The Atomic and Molecular Interactions Gordon Conferences is justifiably recognized for its broad scope, touching on areas ranging from fundamental gas phase and gas-condensed matter collision dynamics, to laser-molecule interactions, photophysics, and unimolecular decay processes. The meeting has traditionally involved scientists engaged in fundamental research in gas and condensed phases and those who apply these concepts to systems of practical chemical and physical interest. A key tradition in this meeting is the strong mixing of theory and experiment throughout. The program for 2008 conference continues these traditions. At the 2008 AMI GRC, there will be talks in 5 broadly defined and partially overlapping areas of intermolecular interactions and chemical dynamics: (1) Photoionization and Photoelectron Spectroscopy; (2) Molecules in Strong Fields; (3) Photodissociation Dynamics; (4) Astrochemistry; and (5) Reaction Dynamics. These areas encompass many of the most productive and exciting areas of chemical physics, including both reactive and nonreactive processes, intermolecular and intramolecular energy transfer, and photodissociation and unimolecular processes. Gas phase dynamics, van der Waals and cluster studies, laser-matter interactions and multiple potential energy surface phenomena will all be discussed. Limited funds are available to support attendance for students and post-docs. Advisors should email the conference chair requesting such support, …

With both China and the United States relying heavily on coal for electricity, senior government officials from both countries have urged immediate action to push forward technology that would reduce carbon dioxide emissions from coal-fired plants. They discussed possible actions at a high-level workshop in April 2009 at the Harvard Kennedy School jointly sponsored by the Belfer Center's Energy Technology Innovation Policy (ETIP) research group, China's Ministry of Science and Technology, and the Chinese Academy of Sciences. The workshop examined issues surrounding Integrated Gasification Combined Cycle (IGCC) coal plants, which turn coal into gas and remove impurities before the coal is combusted, and the related carbon capture and sequestration, in which the carbon dioxide emissions are captured and stored underground to avoid releasing carbon dioxide into the atmosphere. Though promising, advanced coal technologies face steep financial and legal hurdles, and almost certainly will need sustained support from governments to develop the technology and move it to a point where its costs are low enough for widespread use.

Should this be said again? No cell is an island and in tissue-specificity and cancer, context is supreme. Decades ago, seminal recombination experiments illustrated the dominant role of mammary mesenchyme in directing epithelial development, and strongly suggested that the microenvironment plays a significant role also in the manifestation of carcinoma. More direct evidence for such functions came from a study demonstrating that an unadulterated microenvironment can suppress the malignant phenotype and re-direct tumor cells to give rise to normally functioning tissues and indeed healthy mice. One may wonder why such a stunning finding did not convince the scientific community to pay more attention to the role of context. The answers are complex, not the least of which is that concomitantly with this finding, the roles of oncogenes and mutations were being discovered. That excitement carried the day, specially because no one subsequently determined whether or not these mice generated from malignant cells contained tumorigenic mutations, and no new group reproduced the work. The following decade saw the discovery that even potent oncogenes could be ruled by context, and another couple of decades later it was shown that similar reprogramming of metastatic melanoma by an embryonic microenvironment was possible. There are …

In the traditional non-relativistic bound state calculation, the two-photon decay amplitudes of the P-wave {chi}{sub c0,2} and {chi}{sub b0,2} states depend on the derivative of the wave function at the origin which can only be obtained from potential models. However by neglecting the relative quark momenta, the decay amplitude can be written as the matrix element of a local heavy quark field operator which could be obtained from other processes or computed with QCD sum rules technique or lattice simulation. Following the same line as in recent work for the two-photon decays of the S-wave {eta}{sub c} and {eta}{sub b} quarkonia, we show that the effective Lagrangian for the two-photon decays of the P-wave {chi}{sub c0,2} and {chi}{sub b0,2} is given by the heavy quark energy-momentum tensor local operator or its trace, the {anti Q}Q scalar density and that the expression for {chi}{sub c0} two-photon and two-gluon decay rate is given by the f{sub {chi}{sub c0}} decay constant and is similar to that of {eta}{sub c} which is given by f{sub {eta}{sub c}}. From the existing QCD sum rules value for f{sub {chi}{sub c0}}, we get 5 keV for the {chi}{sub c0} two-photon width, somewhat larger than measurement, but possibly …

In 2008, we sampled migrating juvenile Pacific salmonids Oncorhynchus spp. tagged with passive integrated transponder (PIT) tags using a surface pair trawl in the upper Columbia River estuary (rkm 61-83). The cod-end of the trawl was replaced with a cylindrical PIT-tag detection antenna with an 86-cm-diameter fish-passage opening and two detection coils connected in series. The pair trawl was 105 m long with a 91.5-m opening between the wings and a sample depth of 4.9 m. Also during 2008, we finalized the development of a prototype 'matrix' antenna, which was larger than previous antennas by a considerable magnitude. The matrix antenna consisted of 6 coils: a 3-coil front component and a 3-coil rear component, which were separated by 1.5-m of net mesh. The fish-passage opening was 2.5 m wide by 3.0 m tall and was attached to a standard-size pair trawl net. Intermittent sampling with a single crew began on 7 March and targeted yearling Chinook salmon O. tshawytscha and steelhead O. mykiss. Daily sampling using two crews began on 30 April and continued through 14 June; during this period we detected 2.7% of all juvenile salmonids previously detected at Bonneville Dam--a measure of sample efficiency. Sampling with a single …

Maintenance of organ homeostasis and control of appropriate response to environmental alterations requires intimate coordination of cellular function and tissue organization. An important component of this coordination may be provided by proteins that can serve distinct, but linked, functions on both sides of the plasma membrane. Here we present a novel hypothesis in which non-classical secretion can provide a mechanism through which single proteins can integrate complex tissue functions. Single genes can exert a complex, dynamic influence through a number of different processes that act to multiply the function of the gene product(s). Alternative splicing can create many different transcripts that encode proteins of diverse, even antagonistic, function from a single gene. Posttranslational modifications can alter the stability, activity, localization, and even basic function of proteins. A protein can exist in different subcellular localizations. More recently, it has become clear that single proteins can function both inside and outside the cell. These proteins often lack defined secretory signal sequences, and transit the plasma membrane by mechanisms separate from the classical ER/Golgi secretory process. When examples of such proteins are examined individually, the multifunctionality and lack of a signal sequence are puzzling - why should a protein with a well known …

PTEN is a dual function phosphatase with tumor suppressor function compromised in a wide spectrum of cancers. Because tissue polarity and architecture are crucial modulators of normal and malignant behavior, we postulated that PTEN may play a role in maintenance of tissue integrity. We used two non-malignant human mammary epithelial cell lines (HMECs) that form polarized, growth-arrested structures (acini) when cultured in 3-dimensional laminin-rich extracellular matrix gels (3D lrECM). As acini begin to form, PTEN accumulates in both the cytoplasm, and at cell-cell contacts where it colocalizes with E-cadherin/{beta}-catenin complex. Reduction of PTEN levels by shRNA in lrECM prevents formation of organized breast acini and disrupts growth arrest. Importantly, disruption of acinar polarity and cell-cell contact by E-cadherin function-blocking antibodies reduces endogenous PTEN protein levels and inhibits its accumulation at cell-cell contacts. Conversely, in SKBR3 breast cancer cells lacking endogenous E-cadherin expression, exogenous introduction of E-cadherin gene causes induction of PTEN expression and its accumulation at sites of cell interactions. These studies provide evidence that E-cadherin regulates both the PTEN protein levels and its recruitment to cell-cell junctions in 3D lrECM indicating a dynamic reciprocity between architectural integrity and the levels and localization of PTEN. This interaction thus appears to …

This paper presents an initial study that is intended to explore the development of a scalable fully-implicit stabilized unstructured finite element (FE) capability for low-Mach-number resistive MHD. The discussion considers the development of the stabilized FE formulation and the underlying fully-coupled preconditioned Newton-Krylov nonlinear iterative solver. To enable robust, scalable and efficient solution of the large-scale sparse linear systems generated by the Newton linearization, fully-coupled algebraic multilevel preconditioners are employed. Verification results demonstrate the expected order-of-acuracy for the stabilized FE discretization of a 2D vector potential form for the steady and transient solution of the resistive MHD system. In addition, this study puts forth a set of challenging prototype problems that include the solution of an MHD Faraday conduction pump, a hydromagnetic Rayleigh-Bernard linear stability calculation, and a magnetic island coalescence problem. Initial results that explore the scaling of the solution methods are presented on up to 4096 processors for problems with up to 64M unknowns on a CrayXT3/4. Additionally, a large-scale proof-of-capability calculation for 1 billion unknowns for the MHD Faraday pump problem on 24,000 cores is presented.

This report provides a brief historical background of Chile, examines recent political and economic developments, and addresses issues in U.S.-Chilean relations.