Since the inauguration of the Off-Site Animal Investigation project n 1957, there has been one annual report rendered as of 30 June 1958. this is the second annual report dated as of 30 June 1959. The objectives of the project have been unchanged during the past year. These are (1) to enhance the Nevada Test Site/off-site rancher relationship through an active investigation project in their interests, and (2) to provide further information as to the status of the off-site animals in their environment, with special emphasis on their radioactive isotope uptake from fall-out. isotope uptake of the animals is being emphasized as well as the gross and microscopic histopathological examinations. Two year's existence in an area of radio-contamination where a full fission spectrum of isotopes can be found, where radiation background reading range from 1/2 mr per hour to 1 = per hour, has produced no noticeable effect on the animals.