Sediment microbial community biomass and activity in Santa Monica Basin, a nearshore basin in the California Continental Borderland, were examined in October 1985, 1986 and 1987, May 1986, April 1987 and January 1990. Millimeter-scale ATP profiles and incubation of intact cores with {sup 3}H-adenine indicated a high-biomass interface microbial population in the low-oxygen central basin, which was absent in samples from the basin slope sediments. A majority of microbial activity and organic matter mineralization occurred in the top cm of sediment. Comparison of measured ATP and total organic carbon profiles suggest that the C:ATP ratio (wt:wt) ranges between 47:1 and 77:1 in central basin interfacial populations, substantially lower than reported for other aquatic environments. Carbon production estimated from DNA synthesis measurements via {sup 3}H-adenine incorporation was compared with TCO{sub 2} fluxes measured by in situ benthic chamber experiments. Within the uncertainty of the C:ATP ratio, an overall microbial carbon assimilation efficiency of 75--90% was indicated. The low C:ATP ratios and high carbon assimilation efficiencies significantly affect estimates of microbial growth and respiration and are substantially different than those often assumed in the literature. These results suggest that without independent knowledge of these ratios, the uncertainty in tracer-derived microbial growth and …

This subcontract was for Optomec`c support of the Los Alamos National Laboratory`s (LANL`s) Group MEE-12 in the technical specialty area of opto-mechanical design, engineering and fabrication. Two individual tasks were defined by MEE-12 and completed by Optomec personnel. Edward J. Yavornik acted as Principal Investigator on the Wire and Fluorescent Fiber Offset Grid (WAFFOG) for the Neutral Particle Beam (NPB) GTA (Ground Test Accelerator) Experiment, and Thomas Swann acted as Principal Investigator on the ESS-7 Photometers Project. Some hardware was procured/fabricated for the ESS-7 Photometer task, however, most of the work consisted of design and engineering support resulting in drawings and specifications which were prepared by MEE-12 personnel. There were no technical papers or patents generated by Optomec personnel as a result of this work, and all work defined in the contract was completed.