This report documents the findings from an onsite audit of the John Seiberling Federal building located in Akron, Ohio. The Federal landlord for this building is the General Services Administration (GSA). The focus of the audit was to identify various no-cost or low-cost energy efficiency opportunities that, once implemented, would reduce in either electrical and gas consumption and increase the operational efficiency of the building. This audit also provided an opportunity to identify potential capital cost projects that should be considered in the to acquire additional energy (electric and gas) and water savings to further increase the operational efficiency of the building.

Since the emerging of second generation sequencing technologies, the evaluation of different sequencing approaches and their assembly strategies for different types of genomes has become an important undertaken. Next generation sequencing technologies dramatically increase sequence throughput while decreasing cost, making them an attractive tool for whole genome shotgun sequencing. To compare different approaches for de-novo whole genome assembly, appropriate tools and a solid understanding of both quantity and quality of the underlying sequence data are crucial. Here, we performed an in-depth analysis of short-read Illumina sequence assembly strategies for bacterial and archaeal genomes. Different types of Illumina libraries as well as different trim parameters and assemblers were evaluated. Results of the comparative analysis and sequencing platforms will be presented. The goal of this analysis is to develop a cost-effective approach for the increased throughput of the generation of high quality microbial genomes.

This report identifies and briefly summarizes the 20 budget reconciliation measures enacted into law during the period covering 1980, when reconciliation procedures first were used by both chambers, through 2010, the last year of the 111th Congress.

Other written product issued by the Government Accountability Office with an abstract that begins "This document is an E-supplement to GAO-10-390. The 2000 elections in Maine and Arizona were the first in the nation's history where candidates seeking state legislative seats had the option to fully fund their campaigns with public moneys. In 2003, GAO reviewed the public financing programs in Maine and Arizona and found the programs' goals were to (1) increase electoral competition; (2) increase voter choice; (3) curb increases in campaign costs; (4) reduce interest group influence; and (5) increase voter participation. GAO reported that while the number of candidates who participated in the programs increased from 2000 to 2002, it was too soon to determine the extent to which these five goals of the programs were being met. Senate Report 110-129 directed GAO to update its 2003 report. This report: (1) provides data on candidate participation and (2) describes changes in five goals of Maine's and Arizona's programs in the 2000 through 2008 elections and the extent to which changes could be attributed to the programs. To address its objectives, GAO analyzed available data about candidate participation, election outcomes, and campaign spending for the 1996 through …

A letter report issued by the Government Accountability Office with an abstract that begins "The 2000 elections in Maine and Arizona were the first in the nation's history where candidates seeking state legislative seats had the option to fully fund their campaigns with public moneys. In 2003, GAO reviewed the public financing programs in Maine and Arizona and found the programs' goals were to (1) increase electoral competition; (2) increase voter choice; (3) curb increases in campaign costs; (4) reduce interest group influence; and (5) increase voter participation. GAO reported that while the number of candidates who participated in the programs increased from 2000 to 2002, it was too soon to determine the extent to which these five goals of the programs were being met. Senate Report 110-129 directed GAO to update its 2003 report. This report: (1) provides data on candidate participation and (2) describes changes in five goals of Maine's and Arizona's programs in the 2000 through 2008 elections and the extent to which changes could be attributed to the programs. To address its objectives, GAO analyzed available data about candidate participation, election outcomes, and campaign spending for the 1996 through 2008 legislative elections in both states, reviewed …

This report discusses the current status of Central Asian states and U.S. policy, which has been aimed at facilitating their cooperation with U.S. and NATO stabilization efforts in Afghanistan and their efforts to combat terrorism, proliferation, and trafficking in arms, drugs, and persons. Congress will be considering an increase in support for the programs in Central Asia.

This report provides background information and oversight issues for Congress on the Coast Guard's Deepwater acquisition programs for replacing and modernizing the service's aging fleet of deepwater-capable ships and aircraft.

This report provides background information and issues for Congress on the modernization of the Coast Guard's polar icebreaker fleet, which performs a variety of missions supporting U.S. interests in polar regions.

The High Level Radioactive Waste (HLW) Sludge in Savannah River Site (SRS) waste tanks was produced over a period of over 60 years by neutralizing the acidic waste produced in the F and H Separations Canyons with sodium hydroxide. The HLW slurries have been stored at free hydroxide concentrations above 1 M to minimize the corrosion of the carbon steel waste tanks. Sodium nitrite is periodically added as a corrosion inhibitor. The resulting waste has been subjected to supernate evaporation to minimize the volume of the stored waste. In addition, some of the waste tanks experienced high temperatures so some of the waste has been at elevated temperatures. Because the waste is radioactive, the waste is transforming through the decay of shorter lived radioactive species and the radiation damage that the decay releases. The goal of the Savannah River National Laboratory (SRNL) simulant development program is to develop a method to produce a sludge simulant that matches both the chemical and physical characteristics of the HLW without the time, temperature profile, chemical or radiation exposure of that of the real waste. Several different approaches have been taken historically toward preparing simulated waste slurries. All of the approaches used in the …

Report reviewing the management and performance of the Cushing Independent School District's (CISD's) educational, financial, and operations functions.

The 2009 Participating Load Pilot Customer Impact Evaluation provides evidence that short duration demand response events which cycle off air conditioners for less than thirty minutes in a hot, dry environment do not lead to a significant degradation in the comfort level of residents participating in the program. This was investigated using: (1) Analysis of interval temperature data collected from inside residences of select program participants; and (2) Direct and indirect customer feedback from surveys designed and implemented by Southern California Edison at the conclusion of the program season. There were 100 indoor temperature monitors that were acquired by LBNL for this study that transmitted temperature readings at least once per hour with corresponding timestamps during the program season, June-October, 2009. Recorded temperatures were transferred from the onsite telemetry devices to a mesh network, stored, and then delivered to KEMA for analysis. Following an extensive data quality review, temperature increases during each of the thirty demand response test events were calculated for each device. The results are as follows: (1) Even for tests taking place during outside temperatures in excess of 100 degrees Fahrenheit, over 85 percent of the devices measured less than a 0.5 degree Fahrenheit temperature increase indoors …

Correspondence issued by the Government Accountability Office with an abstract that begins "The military exchange services purchase alcoholic beverages for resale on military installations as part of their mission to provide quality goods and services at competitive low prices to their customers--primarily military service members and their families. The revenue generated from the retail sale of products, including alcoholic beverages, supports most of the operating costs of the exchanges and military package stores as well as Morale, Welfare, and Recreation programs. Such programs generally provide for the physical, cultural, and social needs of service members and their families, and include fitness centers, child development services, libraries, and recreation centers. As primarily self-supporting enterprises, military exchanges and package stores are funded predominantly with nonappropriated funds, such as cash and other assets generated through business operations and sales to Department of Defense (DOD) authorized patrons, but certain administrative and support costs of the exchanges are paid by DOD using appropriated funds. In Guam, the Navy Exchange Service Command (NEXCOM) operates exchange activities on Naval Base Guam and the Army and Air Force Exchange Service (AAFES) operates exchange activities on Andersen Air Force Base. Section 652 of the National Defense Authorization Act for …

A letter report issued by the Government Accountability Office with an abstract that begins "The Veterans Benefits, Health Care, and Information Technology Act of 2006 (the 2006 Act) requires the Department of Veterans Affairs (VA) to give priority to veteran-owned and service-disabled veteran-owned small businesses (VOSB and SDVOSB) when awarding contracts to small businesses. The 2006 Act also requires GAO to conduct a 3-year study of VA's implementation of the act. GAO evaluated (1) the extent to which VA met its prime contracting goals for VOSBs and SDVOSBs in fiscal years 2007-2009; (2) VA's progress in implementing procedures to verify the ownership, control, and status of VOSBs and SDVOSBs in its mandated database; and (3) VA's progress in establishing a review mechanism of prime contractors' subcontracts with VOSBs and SDVOSBs. GAO obtained and analyzed data on VA's prime and subcontracting accomplishments, and reviewed a sample of verified businesses to identify any deficiencies in VA's verification program."

We have developed two processes with the Biomek FX robot to construct 454 titanium and Illumina libraries in order to meet the increasing library demands. All modifications in the library construction steps were made to enable the adaptation of the entire processes to work with the 96-well plate format. The key modifications include the shearing of DNA with Covaris E210 and the enzymatic reaction cleaning and fragment size selection with SPRI beads and magnetic plate holders. The construction of 96 Titanium libraries takes about 8 hours from sheared DNA to ssDNA recovery. The processing of 96 Illumina libraries takes less time than that of the Titanium library process. Although both processes still require manual transfer of plates from robot to other work stations such as thermocyclers, these robotic processes represent about 12- to 24-folds increase of library capacity comparing to the manual processes. To enable the sequencing of many libraries in parallel, we have also developed sets of molecular barcodes for both library types. The requirements for the 454 library barcodes include 10 bases, 40-60percent GC, no consecutive same base, and no less than 3 bases difference between barcodes. We have used 96 of the resulted 270 barcodes to construct …

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This report briefly discusses the Department of Defense leases of foreign-built ships, the opponents of this practice, and related legislation.

This document provides the appropriations for FY2011. A comparison is shown in the amount of funding given throughout the years and how the President is wanting to decrease the funding presently (2011). The document continues to describe in detail the funding given for clean water and drinking water along with its changes. The appropriations for prominent issues such as, climate change, hazardous waste clean-up, and the restoration of The Great Lakes are also described.

Correspondence issued by the Government Accountability Office with an abstract that begins "This report responds to Congress' request for information on federal funds provided for fiscal years 2002 through 2009 to selected organizations involved in health-related activities and their affiliates: Advocates for Youth, the Guttmacher Institute, the International Planned Parenthood Federation, the Planned Parenthood Federation of America, the Population Council, and the Sexuality Information and Education Council of the United States. Specifically, Congress requested that GAO identify the amount of federal funds provided to the selected organizations and their affiliates for each of fiscal years 2002 through 2009 and the sources of these federal funds (for example, family planning grants authorized under Title X of the Public Health Service Act). Congress also asked us to provide information on expenditures of federal funds reported by these organizations and their affiliates. For this report, GAO identified (1) reported obligations and disbursements of federal funds that the Department of Health and Human Services (HHS) and the United States Agency for International Development (USAID) provided directly to the selected organizations and their affiliates, and (2) expenditures of federal funds reported by organizations and their affiliates in instances in which an organization or an affiliate …

Daily publication of the U.S. Office of the Federal Register contains rules and regulations, proposed legislation and rule changes, and other notices, including "Presidential proclamations and Executive Orders, Federal agency documents having general applicability and legal effect, documents required to be published by act of Congress, and other Federal agency documents of public interest" (p. ii). Table of Contents starts on page iii.

This report provides the information related to the fishery, aquaculture, and marine mammal issues in the 111th Congress

The U. S. Department of Energy’s Intermountain Industrial Assessment Center (IIAC) at the University of Utah has been providing eligible small- and medium-sized manufacturers with no-cost plant assessments since 2001, offering cost-effective recommendations for improvements in the areas of energy efficiency, pollution prevention, and productivity improvement.

Fosmid or BAC end sequencing plays an important role in de novo assembly of large genomes like fungi and plants. However construction and Sanger sequencing of fosmid or BAC libraries are laborious and costly. The current 454 Paired-End (PE) Library and Illumina Jumping Library construction protocols are limited with the gap sizes of approximately 20 kb and 8 kb, respectively. In the attempt to understand the limitations of constructing PE libraries with greater than 30Kb gaps, we have purified 18, 28, 45, and 65Kb sheared DNA fragments from yeast and circularized the ends using the Cre-loxP approach described in the 454 PE Library protocol. With the increasing fragment sizes, we found a general trend of decreasing library quality in several areas. First, redundant reads and reads containing multiple loxP linkers increase when the average fragment size increases. Second, the contamination of short distance pairs (<10Kb) increases as the fragment size increases. Third, chimeric rate increases with the increasing fragment sizes. We have modified several steps to improve the quality of the long span PE libraries. The modification includes (1) the use of special PFGE program to reduce small fragment contamination; (2) the increase of DNA samples in the circularization step …

The Department of Defense (DOD) is placing an increased planning and budgeting emphasis on irregular warfare (IW) operations, such as counterinsurgency operations. In addition, counterterrorism (CT) operations have been a DOD area of emphasis since the terrorist attacks of September 11, 2001. The Navy for several years has carried out a variety of IW and CT activities, and has taken some steps in recent years to strengthen its ability to conduct such activities. This report discusses these activities and potential oversight issues raised by them.

This report presents highlights of the research conducted on NERSC computers in a variety of scientific disciplines during the years 2008-2009. It also reports on changes and upgrades to NERSC's systems and services as well as activities of NERSC staff.