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We have investigated the preequilibrium kinetics of the L-isoleucine activation reaction catalyzed by Ile-tRNA synthetase in the presence of a fluorescent reporter group, 2-p-toluidinylnaphthalene-6-sulfonate, using the stopped-flow technique. It is found that of all the reactants involved, L-isoleucine binds slowest to the enzyme, apparently in a two-step process. The kinetics of the reaction are invariant in the presence of co-reactants, whereas the kinetics for ATP are drastically changed in the presence of Mg{sup 2+} ions. The formation of enzyme bound L-isoleucyl {approx} AMP is conveniently followed at dilute concentrations. The value for the rate constant of formation was determined to be 135 sec{sup -1} and of the reverse process to be 670 sec{sup -1} at pH 8.0 25 C. These values are considerably higher than the rate constant 15 sec{sup -1} of the dissociation reaction for L-isoleucine. The value of the kinetically defined equilibrium constant between the ternary Michaelis-Menten complex and the ternary enzyme-product complex indicates that, at equilibrium, the Michaelis-Menten complex is favored. The effect of temperature has been determined, and a tentative interpretation of the thermodynamic parameters is offered. The zero standard enthalpy and positive entropy for binding of L-isoleucine is consistent with hydrophobic interactions, whereas the enzyme-ligand …

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It was demonstrated that the modified version of the Martin flowsheet can produce SrF/sub 2/ of acceptable quality using a simulated feed of the same composition as the anticipated packaging plant feed. By careful control of operating conditions, strontium losses in the process can be reduced to an acceptable level. Using a nonrepresentative radioactive feed (which contained impurities not present in the simulated feed) high strontium losses were encountered in the feed purification step, although the purification obtained was excellent. The other steps in the process presented no difficulties. Additional tests of the feed purification step will be necessary when a representative feed is available to ensure that strontium losses can be reduced to an acceptable level. The proposed flowsheet differs from the Martin flowsheet in that a feed purification step is used in the proposed process. All the remaining steps in the process are essentially identical and the data and recommendations reported in the Martin publications appear applicable to the proposed process. Based on the results of this study (and including data published by the Martin Company), a detailed flowsheet with recommended operating conditions is given.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Construction of Article 4025b, V.C.S., relating to "cost of publication and mailing" of materials published by the authority of the Parks and Wildlife Department.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Authority of County Taxing Authority to Levy a Fee on a Mixed Beverage Late Hours Permit and Caterer's Permit Pursuant to Article 666-15a1 Texas Penal Code.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Constitutionality of Article 3912k, V.C.S., as a proper delegation of legislative authority to the grievance committee to set salaries of county officers.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Salary of the judge of the Court of Domestic Relation in and for Midland County, Texas.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Whether the County Tax Assessor-Collector is immune from liability regarding the issuance of a certificate of title.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Whether $1.00 special fee required by Art. 7100, Sec. 4, V. C. S., is applicable to certain receipts and certificates processed by County Tax Assessor-Collectors for special taxing districts.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Collin-Denton County Water and Sanitation District powers per Art. 8280-400, V. C. S., to collect and dispose of garbage within the District.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Application pf Section 54.051(m), Texas Education Code, to tuition charges for vocational-technical courses at public junior colleges and related questions.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Validity of contract involving acquisition of Mustang Island by the State if Texas, obligations upon the present Commission and remedies available to seller in case of dishonor by the State.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: May the Texas Parks and Wildlife Department contract for the sale of water to a private individual whose property adjoins a state park?

A variety of position-sensitive single-wire proportional counters with lengths up to 90 cm have been built and tested in the focal plane of the Enge split-pole spectrographs at the Argonne tandem accelerator and cyclotron. This report, which describes the counters, the associated electronics, and the data-handling procedures, is intended as an operating manual for general use of the system. Under ideal experimental conditions, the spatial resolution of the counters is about 1 to 1.5 mm (FWHM), dependent on the energy loss of the incident particle in the counter. The spatial response of the counters is linear to within 0.5% of the active length. Furthermore, the system contains a conventional proportional counter, behind the position counter, designed to provide particle identification based on the energy lost in the counter. Pulses from the two counters are normally analyzed in coincidence to provide excellent particle discrimination. Examples of typical experimental results are also presented.

Report on water wells and groundwater chemical analysis in Sterling County, Texas.

Using the large aperture spectrometer of proposal 144 and the electron beam with the tagging system removed we propose to study deep inelastic electron scattering at incident electron energies between 50 and 150 GeV, for values of q{sup 2} between 1 and 20 Gev{sup 2}/c and {nu} between 10 and 130 GeV. The spectrometer will detect not only the scattered electron but also most of the secondary particles.

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A tabulation is presented of 52,000 experimental resonance parameters, together with appropriate indexes and bibliographic citations. These materials were contained in the LLL Experimental Neutron Cross Section Library as of May 1, 1972. Techniques used to obtain a selected set of resolved and unresolved resonance parameters from the compilation are described. (auth)

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Report on water wells and groundwater chemical analysis in Sutton County, Texas.

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