This research addressed the long term goals of understanding the assembly and organization of hydrogenase enzymes, of reducing them in size and complexity, of determining structure/function relationships, including energy conservation via charge separation across membranes, and in screening for novel H2 catalysts. A key overall goal of the proposed research was to define and characterize minimal hydrogenases that are produced in high yields and are oxygen-resistant. Remarkably, in spite of decades of research carried out on hydrogenases, it is not possible to readily manipulate or design the enzyme using molecular biology approaches since a recombinant form produced in a suitable host is not available. Such resources are essential if we are to understand what constitutes a “minimal” hydrogenase and design such catalysts with certain properties, such as resistance to oxygen, extreme stability and specificity for a given electron donor. The model system for our studies is Pyrococcus furiosus, a hyperthermophile that grows optimally at 100°C, which contains three different nickel-iron [NiFe-] containing hydrogenases. Hydrogenases I and II are cytoplasmic while the other, MBH, is an integral membrane protein that functions to both evolve H2 and pump protons. Three important breakthroughs were made during the funding period with P. furiosus soluble …

New and existing databases will be combined to support development of physically based models of transition temperature shifts (TTS) for high fluence-low flux (φ < 10{sup 11}n/cm{sup 2}-s) conditions, beyond the existing surveillance database, to neutron fluences of at least 1×10{sup 20} n/cm{sup 2} (>1 MeV). All references to neutron flux and fluence in this report are for fast neutrons (>1 MeV). The reactor pressure vessel (RPV) task of the Light Water Reactor Sustainability (LWRS) Program is working with various organizations to obtain archival surveillance materials from commercial nuclear power plants to allow for comparisons of the irradiation-induced microstructural features from reactor surveillance materials with those from similar materials irradiated under high flux conditions in test reactors

This report explains how employer-sponsored insurance can be used to address concerns about health insurance coverage and cost. Then, it summarizes the three ACA provisions most relevant to small businesses. Also, it analyzes these provisions for their potential effects on small businesses. Last, this report presents several approaches that could address some concerns associated with these provisions (particularly the employer penalty).

This report provides a brief overview of the existing statutory authority and implementing regulation. It describes several policy issues raised in previous debates regarding chemical facility security and identifies policy options for congressional consideration.

A letter report issued by the Government Accountability Office with an abstract that begins "Trends associated with college affinity card agreements include fewer agreements and cardholders and declining payments, according to data GAO analyzed from the Board of Governors of the Federal Reserve System (Federal Reserve) and the Bureau of Consumer Financial Protection (CFPB). The number of affinity card agreements declined from 1,045 in 2009 to 617 in 2012 (41 percent). More than 70 percent of the agreements in 2012 were with institutions of higher education or alumni organizations, and one issuer—FIA Card Services, a subsidiary of Bank of America—had 67 percent of all agreements. Affinity card issuers paid $50.4 million to all organizations in 2012, 40 percent less than in 2009. In most cases, payments were based on numbers of cardholders and the amount spent on the cards. The card agreements covered contractual obligations related to such things as marketing practices, target populations, use of the organization's logo or trademark, terms of payment, and, in some cases, service standards."

This report discusses the Energy and Water Development appropriations bill that provides funding for civil works projects of the Army Corps of Engineers (Corps), for the Department of the Interior's Bureau of Reclamation (Reclamation), the Department of Energy (DOE), and several independent agencies.

The focus of this report is on the federal response to domestic violence under the Family Violence Prevention and Services Act (FVPSA). "Domestic violence" is used in the report to describe violence among intimate partners, including those involved in dating relationships.

This report provides responses to frequently asked questions about health care provided to veterans through the Veterans Health Administration (VHA). It is intended to serve as a quick reference to provide easy access to information. Where applicable, it provides the legislative background pertaining to the question.

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This report provides background information and potential issues for Congress on the Littoral Combat Ship (LCS), a relatively inexpensive Navy surface combatant equipped with modular "plug-and-fight" mission packages for countering mines, small boats, and diesel-electric submarines, particularly in littoral (i.e., near-shore) waters.

This report provides background information regarding the prison population in the United States that has been growing steadily for more than 30 years. The report presents correctional system statistics and discusses the federal government's involvement in offender reentry programs and the Second Chance Act (P.L. 110-199) that was enacted on April 9, 2008.

This report examines the ongoing humanitarian crisis in Syria and the U.S. and international response. It discusses the use of chemical weapons in Syria on August 21, 2013 that triggered an intense debate over possible U.S. military intervention.

This report discusses the current political state of Afghanistan, and the Afghan government. It also discusses Afghanistan's relationship with the United States, particularly U.S. efforts to urge President Hamid Karzai to address corruption within the Afghan government.

A letter report issued by the Government Accountability Office with an abstract that begins "GAO's Cost Estimating and Assessment Guide (Cost Guide) defines 12 leading practices that are associated with four characteristics—comprehensive, well documented, accurate , and credible —that are important to developing high-quality, reliable project-cost estimates. Using the Cost Guide , GAO determined that the Architect of the Capitol's (AOC) cost-estimating guidance conforms to leading practices for developing estimates that are, in general, comprehensive and well-documented . However, AOC's guidance does not substantially conform to leading practices related to developing cost estimates that are accurate and credible . For example, pertaining to the credible characteristic, AOC's guidance does not require determining the confidence level of estimates or quantifying the extent to which a project's costs could vary due to changes in key assumptions. GAO found the strengths and weaknesses of AOC's guidance generally reflected in the cost estimates for AOC's Cannon House Office Building's (Cannon Building) renewal project ($753 million) and Capitol Dome's restoration project ($125 million)."

This report provides references to analytical reports on Cybersecurity from CRS, other Government agencies, trade associations, and interest groups and the information is grouped under topics.

This report describes recent trends in the number of civil service annuitants and the financial status of the Civil Service Retirement and Disability Fund.

Testimony issued by the Government Accountability Office with an abstract that begins "The Department of Veterans Affairs (VA) continues to face long-standing challenges in effectively implementing its information security program. Specifically, from fiscal year 2007 through 2013, VA has consistently had weaknesses in key information security control areas (see table)."

This report is one of a series of reports on legislative process and research; it is intended to serve as a finding aid to sources of information, such as documents, news articles, analysis, contacts and services, used in legislative research.

This report discusses the federal law regarding marijuana that is classified as a Schedule I Controlled Substance. The federal Controlled Substances Act (CSA) outlaws the possession, cultivation, or distribution of marijuana except for authorized research.

This report discusses state medical marijuana laws that grants registered patients, their doctors, and providers immunity from the consequences of state law.

Campaign finance guide for those who file with the Texas Ethics Commission with instructions and guidelines for those running for office.

This report discusses potential policy questions concerning the U.S. Secret Service through an examination of the Service's history and its statutory authorities, mission, and present activities within Department of Homeland Security (DHS).

This report provides information about the Authoritative Reports and Resources on Cyber security.It also lists selected cybersecurity websites for CRS agencies, News, and organizations or institutions.

This report provides background information and issues for Congress on the TAO(X) oiler shipbuilding program, a program to build a new class of fleet oilers for the Navy. The report discusses the issue for Congress which is whether to approve, reject, or modify the Navy's funding requests and acquisition strategy for the TAO(X) program.