This research addressed the long term goals of understanding the assembly and organization of hydrogenase enzymes, of reducing them in size and complexity, of determining structure/function relationships, including energy conservation via charge separation across membranes, and in screening for novel H2 catalysts. A key overall goal of the proposed research was to define and characterize minimal hydrogenases that are produced in high yields and are oxygen-resistant. Remarkably, in spite of decades of research carried out on hydrogenases, it is not possible to readily manipulate or design the enzyme using molecular biology approaches since a recombinant form produced in a suitable host is not available. Such resources are essential if we are to understand what constitutes a “minimal” hydrogenase and design such catalysts with certain properties, such as resistance to oxygen, extreme stability and specificity for a given electron donor. The model system for our studies is Pyrococcus furiosus, a hyperthermophile that grows optimally at 100°C, which contains three different nickel-iron [NiFe-] containing hydrogenases. Hydrogenases I and II are cytoplasmic while the other, MBH, is an integral membrane protein that functions to both evolve H2 and pump protons. Three important breakthroughs were made during the funding period with P. furiosus soluble …

This report discusses the Energy and Water Development appropriations bill that provides funding for civil works projects of the Army Corps of Engineers (Corps), for the Department of the Interior's Bureau of Reclamation (Reclamation), the Department of Energy (DOE), and several independent agencies.

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This report provides information about the early August 2014 U.S.-Africa Leaders Summit in Washington, DC, and policy issues likely to be addressed by participants in the summit and other events being held in conjunction with it.

This report is one of a series of reports on legislative process and research; it is intended to serve as a finding aid to sources of information, such as documents, news articles, analysis, contacts and services, used in legislative research.

This report is one of a series of reports on legislative process and research; it is intended to serve as a finding aid to sources of information, such as documents, news articles, analysis, contacts and services, used in legislative research.

This report examines debates regarding what constitutes an "unfunded federal mandate" and the Unfunded Mandates Reform Act of 1995's (UMRA) implementation, requirements, and costs. It also assesses UMRA's impact on federal mandates and arguments concerning UMRA's future.

The focus of this report is on the federal response to domestic violence under the Family Violence Prevention and Services Act (FVPSA). "Domestic violence" is used in the report to describe violence among intimate partners, including those involved in dating relationships.

This report discusses poverty in United States that varies widely across the population according to age, education, labor force attachment, family living arrangements, and area of residence, among other factors. The report elaborates on the poverty among selected groups, the geography of poverty, and the research supplemental poverty measure.

This report addresses select legal questions raised by the use of military force against the Islamic State.

This report discusses the federal law regarding marijuana that is classified as a Schedule I Controlled Substance. The federal Controlled Substances Act (CSA) outlaws the possession, cultivation, or distribution of marijuana except for authorized research.

This report discusses state medical marijuana laws that grants registered patients, their doctors, and providers immunity from the consequences of state law.

This report informs the congressional debate concerning the Community Reinvestment Act (CRA) effectiveness to incentivize bank lending and investment activity tolow- and moderate-income (LMI) borrowers. After a discussion of the CRA's origins, the examination process and bank activities that are eligible for consideration of CRA credits are presented. Next, the difficulty of determining the CRA's influence on bank behavior is discussed.

This report describes recent trends in the number of civil service annuitants and the financial status of the Civil Service Retirement and Disability Fund.

This report provides background information regarding the prison population in the United States that has been growing steadily for more than 30 years. The report presents correctional system statistics and discusses the federal government's involvement in offender reentry programs and the Second Chance Act (P.L. 110-199) that was enacted on April 9, 2008.

This report, updated through the 113th Congress, discusses the security problem of China's role in weapons proliferation and issues related to the U.S. policy response since the mid-1990s.

This report discusses the current political state of Afghanistan, and the Afghan government. It also discusses Afghanistan's relationship with the United States, particularly U.S. efforts to urge President Hamid Karzai to address corruption within the Afghan government.

This report discusses the reasons that Iran is considered a threat to U.S. security, including Iran's nuclear program, involvement with terrorist organizations, and involvement with neighboring countries' local governments. The report also discusses ways which the U.S. hopes to modify Iran's behavior with sanctions, and the effectiveness of these sanctions.

This report discusses the open economy and society of the United Arab Emirates (UAE), provides a general description of the UAE's government and political structure, and outlines the effects of the recent global economic downturn on the UAE in general and on the city of Dubai in particular. It also describes U.S. concern over the proliferation of advanced technology in relation to a recently-signed U.S.-UAE civilian nuclear agreement, due to the UAE economy and the UAE's lax export controls.

The report discusses recently-enacted legislation and legislation introduced in the 113th Congress related to the Federal Reserve (Fed). It also provides information about the potential impact of greater oversight and disclosure on the Fed's independence and its ability to achieve its macroeconomic and financial stability goals.

This report answers frequently asked questions about the Fire Management Assistance Grant, which authorized by the Robert T. Stafford Disaster Relief and Emergency Assistance Act provides various forms of federal assistance such as the provision of equipment, personnel, and grants to state, local, and tribal governments for the control, management, and mitigation of any fire on certain public or private forest land or grassland that might become a major disaster.

This report explains how employer-sponsored insurance can be used to address concerns about health insurance coverage and cost. Then, it summarizes the three ACA provisions most relevant to small businesses. Also, it analyzes these provisions for their potential effects on small businesses. Last, this report presents several approaches that could address some concerns associated with these provisions (particularly the employer penalty).

This report examines the ongoing humanitarian crisis in Syria and the U.S. and international response. It discusses the use of chemical weapons in Syria on August 21, 2013 that triggered an intense debate over possible U.S. military intervention.

This report discusses relevant portions of the U.S. corporate income tax system and how inversions have commonly been structured. It also looks at how Congress and Department of the Treasury have reduced the benefits of inversions, including The American Jobs Creation Act, as well as post-2004 inversions and treasury regulations, and policy options.