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The EAR-motif of the Cys2/His2-type Zinc Finger Protein Zat7 Plays a Key Role in the Defense Response of Arabidopsis to Salinity Stress (open access)

The EAR-motif of the Cys2/His2-type Zinc Finger Protein Zat7 Plays a Key Role in the Defense Response of Arabidopsis to Salinity Stress

Article reporting that transgenic Arabidopsis plants constitutively expressing the Cys2/His2 zinc finger protein Zat7 have suppressed growth and are more tolerant to salinity stress. Findings demonstrate that the EAR-domain of Cys2/His2-type zinc finger proteins plays a key role in the defense response of Arabidopsis to abiotic stresses.
Date: March 23, 2007
Creator: Ciftci-Yilmaz, Sultan; Morsy, Mustafa R.; Song, Luhua; Coutu, Alicia; Krizek, Beth A.; Lewis, Michael W. et al.
Object Type: Article
System: The UNT Digital Library
Function of Conserved Residues of Human Glutathione Synthetase: Implications for the ATP-Grasp Enzymes (open access)

Function of Conserved Residues of Human Glutathione Synthetase: Implications for the ATP-Grasp Enzymes

Article examining the mechanism of Glutathione synthetase, an enzyme that belongs to the glutathione synthetase ATP-binding domain-like superfamily. A variety of structural alignment methods were applied and four highly conserved residues of human glutathione synthetase (Glu-144, Asn-146, Lys-305, and Lys-364) were identified in the binding site. The function of these was studied by experimental and computational site-directed mutagenesis.
Date: May 21, 2004
Creator: Dinescu, Adriana; Cundari, Thomas R., 1964-; Bhansali, Vikas S.; Luo, Jia-Li & Anderson, Mary E.
Object Type: Article
System: The UNT Digital Library
The Water-Water Cycle Is Essential for Chloroplast Protection in the Absence of Stress (open access)

The Water-Water Cycle Is Essential for Chloroplast Protection in the Absence of Stress

Article showing that knockdown Arabidopsis plants with suppressed expression of the key water-water cycle enzyme, thylakoid-attached copper/zinc superoxide dismutase (KD-SOD), are suppressed in their growth and development. This article provides e genetic evidence for the importance of the water-water cycle in protecting the photosynthetic apparatus of higher plants from photooxidative damage.
Date: October 3, 2003
Creator: Rizhsky, Ludmila; Liang, Hongjian & Mittler, Ron
Object Type: Article
System: The UNT Digital Library
Phosphorylation of Myosin Light Chain Kinase by p21-activated Kinase PAK2 (open access)

Phosphorylation of Myosin Light Chain Kinase by p21-activated Kinase PAK2

Article reporting that PAK2, a member of the Rho family of GTPase-dependent kinases, regulates isometric tension development and myosin II RLC phosphorylation in saponin permeabilized endothelial monolayers. The results demonstrate that PAK2 can directly phosphorylate MLCK, inhibiting its activity and limiting the development of isometric tension.
Date: June 16, 2000
Creator: Goeckeler, Zoe M.; Masaracchia, Ruthann A.; Zeng, Qi; Chew, Teng-Leong; Gallagher, Patricia & Wysolmerski, Robert B.
Object Type: Article
System: The UNT Digital Library
Activation of an S6/H4 Kinase (PAK 65) from Human Placenta by Intramolecular and Intermolecular Autophosphorylation (open access)

Activation of an S6/H4 Kinase (PAK 65) from Human Placenta by Intramolecular and Intermolecular Autophosphorylation

Article proposing a model in which phosphorylation of sites 1 and 2 occurs sequentially. The model proposes that trypsin treatment of the inactive holoenzyme removes an inhibitory rac-binding domain which blocks MgATP access to the catalytic site. The pseudosubstrate domain at site 1 is autophosphorylated and subsequent bimolecular autophosphorylation at site 2 fully opens the catalytic site.
Date: September 8, 1995
Creator: Benner, Gretchen E.; Dennis, Patrick B. & Masaracchia, Ruthann A.
Object Type: Article
System: The UNT Digital Library
Activation of an S6 Kinase From Human Placenta by Autophosphorylation (open access)

Activation of an S6 Kinase From Human Placenta by Autophosphorylation

Article describing a novel S6 kinase isolated from human placenta which autoactivates through autophosphorylation in vitro. The data supports the hypothesis that this previously uncharacterized S6 kinase belongs to a unique family of protein kinases which utilize autophosphorylation as part of their in vivo activation mechanism.
Date: September 15, 1993
Creator: Dennis, Patrick B. & Masaracchia, Ruthann A.
Object Type: Article
System: The UNT Digital Library
Molecular cloning and characterization of a gene encoding pea cytosolic ascorbate peroxidase (open access)

Molecular cloning and characterization of a gene encoding pea cytosolic ascorbate peroxidase

Article isolating a gene encoding cytosolic bate peroxidase (ApxI) from pea (Pisum sativum L.) and determining its nucleotide sequence.
Date: October 25, 1992
Creator: Mittler, Ron & Zilinskas, B.A.
Object Type: Article
System: The UNT Digital Library
Multisite phosphorylation of a synthetic peptide derived from the carboxyl terminus of the ribosomal protein S6 (open access)

Multisite phosphorylation of a synthetic peptide derived from the carboxyl terminus of the ribosomal protein S6

Article synthesizing and testing the synthetic peptide AKRRRLSSLRASTSKSESSQK (56-21) which corresponds to the carboxyl-terminal 21 amino acids of human ribosomal protein S6 as a substrate for S6/H4 kinase purified from human placenta. The data suggests that multiple S6 kinases may be required to phosphorylate S6 at all five sites which are modified in vivo.
Date: January 5, 1991
Creator: Brandon, Stanley D. & Masaracchia, Ruthann A.
Object Type: Article
System: The UNT Digital Library
The accessory role of the diaphragmaticus muscle in lung ventilation in the estuarine crocodile Crocodylus porosus (open access)

The accessory role of the diaphragmaticus muscle in lung ventilation in the estuarine crocodile Crocodylus porosus

This article explores lung ventilation in crocodilians through direct measurements of ventilation, metabolic rate, and arterial blood gases of juvenile estuarine crocodiles in varying conditions. The results suggest that, in C. porosus, costal ventilation alone is able to support metabolic demands at rest, and the diaphragmaticus is largely an accessory muscle used at times of elevated metabolic demand.
Date: November 17, 2011
Creator: Munns, Suzanne L.; Owerkowicz, Tomasz; Andrewartha, Sarah J. & Frappell, Peter B.
Object Type: Article
System: The UNT Digital Library
Cluster embedding of ionic systems: Point charges and extended ions (open access)

Cluster embedding of ionic systems: Point charges and extended ions

This article presents a detailed analysis of the orbital energies of a representative cluster model of a high spin metal oxide, FeO, that has been made to determine the importance of different embedding schemes to represent the extended oxide crystal. The embedding of cluster models of oxides with point charges and with extensions of the embedding which take into account the spatial extent of the cations is examined with an emphasis on the consequences of this embedding for the relative ionization and excitation energies that are measured in core-level spectroscopies.
Date: May 1, 2019
Creator: Bagus, Paul S.; Sassi, Michael J. & Rosso, Kevin M.
Object Type: Article
System: The UNT Digital Library
Detergentes y Biodetergentes en la Biorremediación de Suelo: Inconsistencias y Evidencias (open access)

Detergentes y Biodetergentes en la Biorremediación de Suelo: Inconsistencias y Evidencias

This article analyzes the bioremediation of the soil contaminated by hydrocarbons (HYCOS), the importance of biostimulation with a detergent (DEGE) or biodetergent (BIODET) and the inconsistencies generated by their application.
Date: August 28, 2020
Creator: Saucedo-Martínez, Blanca Celeste; Márquez-Benavides, Liliana; Santoyo-Pizano, Gustavo & Sánchez-Yáñez, Juan Manuel
Object Type: Article
System: The UNT Digital Library