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Visualization of Lipid Droplet Composition by Direct Organelle Mass Spectrometry
Article reporting an approach that combines the visualization of individual lipid droplets (LDs), microphase extraction of lipid components from droplets, and the direct identification of lipid composition by nanospray mass spectrometry, even to the level of a single LD. The triacylglycerol (TAG) composition of LDs from several plant sources (mature cotton (Gossypium hirsutum) embryos, roots of cotton seedlings, and Arabidopsis thaliana seeds and leaves) were examined by direct organelle mass spectrometry and revealed the heterogeneity of LDs derived from different plant tissue sources.
Date: February 4, 2011
Creator: Horn, Patrick J.; Ledbetter, Nicole R.; James, Christopher N.; Hoffman, William D.; Case, Charlene R.; Verbeck, Guido F. et al.
System: The UNT Digital Library