This article uses a discovery driven comparative proteomics analysis to reveal unique early signaling response of the yeast Saccharomyces cerevisiae on the proteome level to oxidants with a different mechanism of action as early as 3 min after treatment with four oxidants, namely H₂O₂, cumene hydroperoxide (CHP), and menadione and diamide, when protein abundances were compared using label-free quantification relying on a high-resolution mass analyzer (Orbitrap).

This article considers areas of cardiovascular research that have benefited from studies of the chorioallantoic membrane (CAM), including the themes of investigation of the CAM’s hemodynamic influence on heart and central vessel development, use of the CAM as a model vascular bed for studying angiogenesis, and the CAM as an assay tool.

Article reporting a chromosome-scale assembly of the T. sinense genome.

Article studying climate adaptation using common snapping turtles, Chelydra serpentina, as the model species. The genome and genetic variants identified here provide a foundation for future studies of adaptation to climate.

Editorial on the research topic of biofuels and bioenergy.

This article tests if it is possible to piggyback more than one antisense deoxyoligonucleotide (dO) with one vivo morpholino (VMO). The authors previously developed a piggyback knockdown method that was used to knockdown genes in adult zebrafish. In this article, the authors develop a method to knockdown three genes at one time, and by increasing the concentration of VMO by twofold, could knockdown six genes simultaneously. These multiple gene knockdowns will not only increase the efficiency of the method in whole genome-wide knockdowns but will also be useful to study multifactorial disorders.

This review article calls for establishing the CaMV 35S promoter as a quantitative reference standard for transcription activity in plants.

This article applies a developmental 3-dimensional critical window experimental design for a vertebrate, using time, temperature, and phenotypic response (i.e., variables measured). To assess thermal effects on fish development, the authors examined trout hatchling phenotypes following rearing in constant temperatures (5, 10, 15 and 17.5 °C) and following exposure to increased temperature above 5 °C during specific developmental windows.

This article finds that the transcript profile of the laccase gene ChLAC8 parallels the accumulation of C-lignin during seed coat development. Recombinant ChLAC8 oxidizes caffeyl and sinapyl alcohols, generating their corresponding dimers or trimers in vitro, but cannot oxidize coniferyl alcohol. The authors propose a basis for this substrate preference based on molecular modeling/docking experiments. Suppression of ChLAC8 expression led to significantly reduced C-lignin content in the seed coats of transgenic Cleome plants. Feeding of 13C-caffeyl alcohol to the Arabidopsis (Arabidopsis thaliana) caffeic acid o-methyltransferase mutant resulted in no incorporation of 13C into C-lignin, but expressing ChLAC8 in this genetic background led to appearance of C-lignin with >40% label incorporation. These results indicate that ChLAC8 is required for C-lignin polymerization and determines lignin composition when caffeyl alcohol is available.

Article aims to identify other proteases that may activate FVII using zebrafish as a model.

Article presents a method that provides a novel way to synthesize NPs and expose biological organisms for various toxicological analysis.

Article filling the regional gap in knowledge of diet composition of the barn owl (Tyto alba) and the long-eared owl (Asio otus). The study was conducted at the Lewisville Lake Environmental Learning Area (LLELA) and examined the remains found in owl pellets collected September 2016 to March 2017.

This article develops and validates a sequential protocol for quantifying reserve and structural carbohydrates in soybean seed. It was successfully applied to eight soybean genotypes harvested from two locations, and the resulting correlations of carbohydrate and oil or protein are presented. This methodology has the potential not only to guide soybean cultivar optimization processes but also to be expanded to other crops with only slight modifications.

Article investigating the transgenerational inheritance from both parental lines in zebrafish. Adult zebrafish were separated into female and male groups exposed for 21 days to either a control diet or to a diet containing water accommodated fractions of crude oil. To determine the maternal and paternal influence on their offspring, the authors evaluated responses from molecular to whole organismal levels in both generations.

Article discussing key questions and opportunities in the field of Proanthocyanidins (PA) biosynthesis.

This article explores the relationship among MYB30, the ROS wave, and systemic acclimation in Arabidopsis by determining the SAA response to HL stress of MYB30 mutants and wild-type plants.

Article demonstrating that part of the function of two SEIPIN isoforms in Arabidopsis (Arabidopsis thaliana), AtSEIPIN2 and AtSEIPIN3, may depend on their interaction with the vesicle-associated membrane protein (VAMP)–associated protein (VAP) family member AtVAP27-1. The authors used a combination of biochemical, cell biology, and genetics approaches to show that AtVAP27-1 interacts with the N termini of AtSEIPIN2 and AtSEIPIN3 and likely supports the normal formation of LDs. This insight indicates that the ER membrane tethering machinery in plant cells could play a role with select SEIPIN isoforms in LD biogenesis at the ER, and additional experimental evidence in Saccharomyces cerevisiae supports the possibility that this interaction may be important in other eukaryotic systems.

Article is a study with the goal of optimizing and validating the use of a colorimetric plate-based respiratory burst and fluorometric plate-based phagocytic cell activity assays for use with kidney cells from the fathead minnow (Pimephales promelas), an emerging immunotoxicity model.

Article presents a study where whole transcriptome sequencing (RNA-seq) and differential expression analysis were used to identify early (1–3 hr) transcriptional changes induced by the exogenous treatment of NAE 18:2 and NAE 18:3 in cotyledons, roots, and seedlings.

This article shows that the activity of N-linolenoylethanolamine (NAE 18:3) requires an intact G-protein complex. Specifically, genetic ablation of the Gβγ dimer or loss of the full set of atypical Gα subunits strongly attenuates an NAE-18:3–induced degreening of cotyledons in Arabidopsis (Arabidopsis thaliana) seedlings. This effect involves, at least in part, transcriptional regulation of chlorophyll biosynthesis and catabolism genes. In addition, there is feedforward transcriptional control of G-protein signaling components and G-protein interactors. These results are consistent with NAE 18:3 being a lipid signaling molecule in plants with a requirement for G-proteins to mediate signal transduction, a situation similar, but not identical, to the action of NAE endocannabinoids in animal systems.

Article presents novel insights into the molecular nature of the SH genes, thereby opening new avenues for coffee rust resistance molecular breeding.

Article measuring lipid levels with mass spectrometry to confirm the previously observed changes in Arabidopsis thaliana leaf lipids under three heat stress regimens. Additionally, in response to heat, increased oxidation of the fatty acyl chains of leaf galactolipids, sulfoquinovosyldiacylglycerols, and phosphatidylglycerols, and incorporation of oxidized acyl chains into acylated monogalactosyldiacylglycerols are shown. The authors also observed increased levels of digalactosylmonoacylglycerols and monogalactosylmonoacylglycerols. The hypothesis that a defect in sterol glycosylation would adversely affect regrowth of plants after a severe heat stress regimen was tested, but differences between wild-type and sterol glycosylation-defective plants were not detected.

This article is a commentary on coordinated use of methyl marks to remodel both the epigenome and the (epi)cytoskeleton, which opens the possibility for integrated regulation (referred to as ‘epiregulation’) of other higher-level functions, such as muscle contraction or learning and memory, and could even have evolutionary implications.

Article reports observations of two Subarctic North Pacific puffin species in the North Atlantic near the coast of north-west Greenland.