Article examines the creation of a transformation pipeline (TP) to produce plants with decreased recalcitrance and a laboratory information management system (LIMS) for data compilation from these plants. While many genes accepted into the TP resulted in transgenic switchgrass without modified lignin or biomass content, a group of genes with potential to improve lignocellulosic biofuel yields was identified. Results from transgenic lines targeting xyloglucan and lignin structure provide examples of the types of information available on switchgrass lines produced within BioEnergy Science Center (BESC).

This article provides information on the organization and outcomes of the BioEnergy Science Center Transformation Pipeline, and supplies useful information when developing coordinated, large-scale, multi-institutional reverse genetic pipelines to improve crop traits.

This article is part of a Focus Issue on the topic of Rubin LSST Survey Strategy Optimization. The motivation, history, and decision-making process of the observing strategy optimization are detailed, giving context to the science-driven proposals and recommendations for the survey strategy included in this Focus Issue.