Article asserts that the Lesser Prairie-Chicken (Tympanuchus pallidicinctus; LEPC) is an iconic North American prairie grouse, renowned for ornate and spectacular breeding season displays, a species that has been declining in population across much of its historical range, with corresponding precipitous declines in contemporary population abundance, largely due to climatic and anthropogenic factors. The authors describe an annotated reference genome that was generated from a LEPC sample collected from the southern DPS.