Partner
Collection
UNT Theses and Dissertations
1,507
Texas Digital Newspaper Program
601
The Greensheet
253
Denton County Newspapers Collection
179
Denton Record-Chronicle
152
UNT Scholarly Works
102
UNT Open Access Symposium
55
Texas Jewish Post
39
The Suburbia News
23
Texas Jewish Herald /Jewish Herald /Jewish Herald-Voice
20
27 More
Degree Department
Department of Teacher Education and Administration
110
College of Music
90
Department of Psychology
81
Department of Counseling and Higher Education
75
Department of English
70
Department of Biological Sciences
67
Department of Computer Science and Engineering
61
Department of Chemistry
59
Department of Behavior Analysis
56
Department of Learning Technologies
49
103 More
Degree Discipline
Degree Level
Country
States
Decade
Month
Language
2,315 Matching Results
Results open in a new window/tab.
Exploring Simscape™ Modeling for Piezoelectric Sensor Based Energy Harvester
This work presents an investigation of a piezoelectric sensor based energy harvesting system, which collects energy from the surrounding environment. Increasing costs and scarcity of fossil fuels is a great concern today for supplying power to electronic devices. Furthermore, generating electricity by ordinary methods is a complicated process. Disposal of chemical batteries and cables is polluting the nature every day. Due to these reasons, research on energy harvesting from renewable resources has become mandatory in order to achieve improved methods and strategies of generating and storing electricity. Many low power devices being used in everyday life can be powered by harvesting energy from natural energy resources. Power overhead and power energy efficiency is of prime concern in electronic circuits. In this work, an energy harvester is modeled and simulated in Simscape™ for the functional analysis and comparison of achieved outcomes with previous work. Results demonstrate that the harvester produces power in the 0 μW to 100 μW range, which is an adequate amount to provide supply to low power devices. Power efficiency calculations also demonstrate that the implemented harvester is capable of generating and storing power for low power pervasive applications.
Date:
May 2017
Creator:
Dhayal, Vandana
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
High School Teachers' Perceptions of Giftedness, Gifted Education, and Talent Development
In the field of gifted education, there is little research on the perceptions of high school teachers of the gifted about giftedness, good gifted education practices, and the nature and needs of gifted learners. The purpose of this study was to form a deeper understanding of how those educators who guide gifted learners out of high school and into adulthood perceive giftedness and gifted education. This qualitative study, conducted in two phases, took place in a large suburban school district with three large high school systems and was focused on the responses of high school teachers to assess their attitudes, feelings, and opinions about the nature and needs of gifted learners using a grounded theory model of analysis. Data collected from the 11 participants in the first phase of the analysis was combined with that collected from the 13 participants in phase two and validated throughout with continual comparison through memoing. Participants reported a general lack of engagement with scholarly work in the gifted education field as well as a dependence on the school district for effective training in classroom practice. Evidence also suggested a view of giftedness among the participants as an inherent quality of some people who needed …
Date:
May 2017
Creator:
Russell, Joseph L.
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
Understanding Antecedents and Outcomes of Co-Creation in Service Innovation Setting
This dissertation uses service-dominant logic to understand customer value creation in service innovation context. Although co-creation became an interesting phenomenon among marketing scholars, the underlying mechanisms of co-creation process are still vague. To fill the gaps in the literature, we draw from S-D logic to understand antecedents and outcome of co-creation to service innovation context. The results of this study show that most of the hypotheses are supported, thus finding support for the overall model of value co-creation.
Date:
May 2017
Creator:
Rokonuzzaman, Md
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
Nonlinear Light Generation from Optical Cavities and Antennae
Semiconductor based micro- and nano-structures grown in a systematic and controlled way using selective area growth are emerging as a promising route toward devices for integrated optical circuitry in optoelectronics and photonics field. This dissertation focuses on the experimental investigation of the nonlinear optical effects in selectively grown gallium nitride micro-pyramids that act as optical cavities, zinc oxide submicron rods and indium gallium nitride multiple quantum well core shell submicron tubes on the apex of GaN micro pyramids that act as optical antennae. Localized spatial excitation of these low dimensional semiconductor structures was optimized for nonlinear optical light (NLO) generation due to second harmonic generation (SHG) and multi-photon luminescence (MPL). The evolution of both processes are mapped along the symmetric axis of the individual structures for multiple fundamental input frequencies of light. Effects such as cavity formation of generated light, electron-hole plasma generation and coherent emission are observed. The efficiency and tunability of the frequency conversion that can be achieved in the individual structures of various geometries are estimated. By controlling the local excitation cross-section within the structures along with modulation of optical excitation intensity, the nonlinear optical process generated in these structures can be manipulated to generate coherent light …
Date:
May 2017
Creator:
Butler, Sween J.
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
Moteino-Based Wireless Data Transfer for Environmental Monitoring
Data acquisition through wireless sensor networks (WSNs) has enormous potential for scalable, distributed, real-time observations of monitored environmental parameters. Despite increasing versatility and functionalities, one critical factor that affects the operation of WSNs is limited power. WSN sensor nodes are usually battery powered, and therefore the long-term operation of the WSN greatly depends on battery capacity and the node's power consumption rate. This thesis focuses on WSN node design to reduce power consumption in order to achieve sustainable power supply. For this purpose, this thesis proposes a Moteino-based WSN node and an energy efficient duty cycle that reduces current consumption in standby mode using an enhanced watchdog timer. The nodes perform radio communication at 915 MHz, for short intervals (180ms) every 10 minutes, and consume 6.8 mA at -14dBm. For testing, the WSN node monitored a low-power combined air temperature, relative humidity, and barometric pressure sensor, together with a typical soil moisture sensor that consumes more power. Laboratory tests indicated average current consumption of ~30µA using these short radio transmission intervals. After transmission tests, field deployment of a star-configured network of nine of these nodes and one gateway node provides a long-term platform for testing under rigorous conditions. A webserver …
Date:
May 2017
Creator:
Iyiola, Samuel
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
The Persuasive Power of Ridicule: A Critical Rhetorical Analysis of Gender and Humor in U.S. Sitcoms
The serious investigation of humor's function in society is an emerging area of research in critical humor studies, a "negative" subsect of the extensive and "positive" research that assumes humor's goodness. Using Michael Billig's theory of ridicule as a framework, this study explored how humor operated to discipline characters who broke social norms or allowed characters to rebel against those norms. Layering this with gender performative theory, the study also investigated how different male and female characters used ridicule and were subject to it themselves. After examining ridicule in The Big Bang Theory, 2 Broke Girls, and The Odd Couple using a critical rhetorical analysis, the findings revealed that disciplinary ridicule was used more overtly throughout all three programs, while potentially rebellious ridicule emerged in only a few scenes. In addition, men were overwhelmingly the subjects of disciplinary ridicule, although women found themselves as subjects throughout all three programs as well. The discursive ridiculing of non-normative bodies constructed and maintained social norms about gender and sexuality, thereby uninviting these bodies from participating in society.
Date:
May 2017
Creator:
Waters, Leah E.
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
Emerging Adults Delay Mental Illness Treatment: Another Manifestation of Experiential Avoidance?
Emerging adulthood is a term coined to recognize 18 to 25 year-olds who engage in self-exploration while not yet fully identifying as adults. Many emerging adult college students experience stress, anxiety, and depression. Although many colleges provide affordable and available mental health resources for students, many students who need help appear to not utilize these services. Gaining greater understanding of underlying processes that influence psychological treatment-seeking behavior is imperative. The current study sought to explore the role experiential avoidance (EA) plays as a treatment-seeking barrier in the context of emerging adulthood. Undergraduate students completed online measures of emerging adulthood dimensions, psychological symptoms, EA, self-stigma of, perceived public stigma of, intentions to, and attitudes and beliefs towards seeking treatment, treatment seeking behavior, and a demographics questionnaire. Binomial hierarchical logistic regressions and correlational analyses examined the relationship of EA and treatment-seeking behaviors, accounting for known barriers and emerging adult characteristics. After controlling for demographic variables, results indicated that EA was significantly positively correlated with self-stigma (r = .187), p < .001), perceived public stigma (r = .178, p < .001), intentions (r - .207, p < .001), psychological symptoms (r = .713, p < .001), and attitudes and beliefs (r = .009, …
Date:
May 2017
Creator:
Hulsey, Teresa
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
The Status and Challenges of Online Distance Education Programs in Post-Secondary Institutions in Ghana
The purpose of this study was to identify the status and challenges of online distance education programs in post-secondary institutions in Ghana. This study was a replication of a similar study conducted in Kenya in 2009, at the University of Nairobi and Kenyatta University. This present study was conducted with an online survey using Google survey assessment. The survey requested responses from six post-secondary institutions in Ghana. Out of a total of 450 projected student responses, 309 responses were received with a 69% participation rate. A total of 14 responses were received for instructors out of a projected 30 resulting in 47% participation rate. And for administrators, 8 responses were received out of a projected 12 resulting in a 67% participation rate. Overall the study revealed that Ghana post-secondary institutions have established and incorporated online distance education into their programs, offering both online and blended courses. Some of these institutions established regional centers across the country and incorporated foreign instructors into their programs. The survey also revealed that students were satisfied with the overall online distance education program in their institutions which included the level of instruction, feedback and evaluation. However, there were still challenges revealed from the study that …
Date:
May 2017
Creator:
Adjabeng, Stanley
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
Causes of the Jewish Diaspora Revolt in Alexandria: Regional Uprisings from the Margins of Greco-Roman Society, 115-117 CE
This thesis examines the progression from relatively peaceful relations between Alexandrians and Jews under the Ptolemies to the Diaspora Revolt under the Romans. A close analysis of the literature evidences that the transition from Ptolemaic to Roman Alexandria had critical effects on Jewish status in the Diaspora. One of the most far reaching consequences of the shift from the Ptolemies to Romans was forcing the Alexandrians to participate in the struggle for imperial patronage. Alexandrian involvement introduced a new element to the ongoing conflict among Egypt’s Jews and native Egyptians. The Alexandrian citizens consciously cut back privileges the Jews previously enjoyed under the Ptolemies and sought to block the Jews from advancing within the Roman system. Soon the Jews were confronted with rhetoric slandering their civility and culture. Faced with a choice, many Jews forsook Judaism and their traditions for more upwardly mobile life. After the outbreak of the First Jewish War Jewish life took a turn for the worse. Many Jews found themselves in a system that classified them according to their heritage and ancestry, limiting advancement even for apostates. With the resulting Jewish tax (fiscus Judaicus) Jews were becoming more economically and socially marginalized. The Alexandrian Jews were …
Date:
May 2016
Creator:
Vargas, Miguel M.
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
Searching for Songs of the People: The Ideology of the Composers' Collective and Its Musical Implications
The Composers' Collective, founded by leftist composers in 1932 New York City, sought to create proletarian music that avoided the "bourgeois" traditions of the past and functioned as a vehicle to engage Americans in political dialogue. The Collective aimed to understand how the modern composer became isolated from his public, and discussions on the relationship between music and society pervade the radical writings of Marc Blitzstein, Charles Seeger, and Elie Siegmeister, three of the organization's most vocal members. This new proletarian music juxtaposed revolutionary text with avant-garde musical idioms that were incorporated in increasingly greater quantities; thus, composers progressively acclimated the listener to the dissonance of modern music, a distinctive sound that the Collective hoped would become associated with revolutionary ideals. The mass songs of the two Workers' Song Books published by the Collective, illustrate the transitional phase of the musical implementation of their ideology. In contrast, a case study of the song "Chinaman! Laundryman!" by Ruth Crawford Seeger, a fringe member of the Collective, suggests that this song belongs within the final stage of proletarian music, where the text and highly modernist music seamlessly interact to create what Charles Seeger called an "art-product of the highest type."
Date:
May 2018
Creator:
Chaplin-Kyzer, Abigail
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
Perseverance in the Face of Totalitarianism: The Life and Legacy of Józef Zygmunt Szulc in Nazi Occupied France
The Reichsleiter Rosenberg Task Force of 1940, initiated a systematic confiscation of items belonging to Jews throughout Europe. Because of this task force and Hitler's decrees, Jews across Europe were labeled as stateless, and were stripped of ownership and rights to property. Not only did these actions devastate Jews economically, but intellectually and artistically as well. In parts of occupied France, this task force was legitimized by Vichy laws under the label of the Commissariat Générale aux Questions Juives (General Commission for Jewish Issues) and enabled Nazi officials to closely watch Jewish musicians and stop them from performing their music, profiting from anyone else performing it, and to halt any public performance of Jewish compositions. This dissertation exhibits the lost legacy of one such Jewish musician, Józef Szulc. It discusses him as a musician of great importance in the ongoing recovery of Jewish culture, music, and life during World War II. His musical output has historical notoriety, as seen through reviews and performance history. The study of Vichy laws and their effect on Jewish musicians in Paris during the Nazi occupation provides the socio-political context for Szulc's life. It also provides the most plausible reason why his contribution to French …
Date:
May 2019
Creator:
Mamola, Bethany Grace
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
El Teatro Dominicano: Instrumento Político y Voz de una Identidad
Throughout the history of the Dominican Republic, theater has played an instrumental role in the cultural life its people, one which transcends purely artistic and cultural dimensions extending its influence into the political and social fabric of the nation. In spite of Spanish colonization and later Haitian occupation, a nascent national identity began forming early on. The staging of certain plays exposed latent conflicts and revealed sectorial, class interests. Theater provided a means of expression for popular sentiments, thus revealing an urge by the people to manifest their concerns, usually under the heavy weight of censorship. This thesis focuses on key moments of the first 140 years of Dominican Republic theater. It is organized into three chapters: "Historical Antecedents", "Theater of the Dictatorship" and "Theater of the Post-Dictatorship." The first chapter deals with the struggle for independence through 1844; the next focuses on the theatrical plays and political climate of bloody Rafael Leonidas Trujillo dictatorship which spanned from 1930 to his assassination in 1961, and the third presents the theater that appeared in the subsequent years of the equally repressive Joaquin Balaguer presidency (1966-1978). The analysis of these key historical moments, in conjunction with the dramaturgy of playwrights such as …
Date:
May 2016
Creator:
Curiel, Sandra Y.
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
Mujeres Sumisas, Mujeres Transgresoras en el Siglo XIX Español: una Aproximación a la Obra de Francisca Navarro y Joaquina García Balmaseda
This research discusses the changing role of women in Spanish society during the nineteenth century through the works of Francisca Navarro and Joaquina Garcia Balmaseda. The thesis shows the break with the traditional image of the "angel del hogar" and the gradual incorporation of the social changes affecting women which were reflected in the female protagonists of the comedies written by such playwrights. By reading theoretical texts of the emerging feminist theory and through analysis of the main female characters, this study examines the changes regarding the established canon and its own evolution within the nineteenth century, from the works of a pioneer like Francisca Navarro in the first third of the century to those of Joaquina Gracia Balmaseda towards the end of it.
Date:
May 2016
Creator:
Zaera, Isabel María
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
Naples and the Emergence of the Tenor as Hero in Italian Serious Opera
The dwindling supply of castrati created a crisis in the opera world in the early 19th century. Castrati had dominated opera seria throughout the 18th century, but by the early 1800s their numbers were in decline. Impresarios and composers explored two voice types as substitutes for the castrato in male leading roles in serious operas: the contralto and the tenor. The study includes data from 242 serious operas that premiered in Italy between 1800 and 1840, noting the casting of the male leading role for each opera. At least 67 roles were created for contraltos as male heroes between 1800 and 1834. More roles were created for tenors in that period (at least 105), but until 1825 there is no clear preference for tenors over contraltos except in Naples. The Neapolitan preference for tenors is most likely due to the influence of Bourbon Kings who sought to bring Enlightenment values to Naples. After the last castrato retired in 1830 and the casting of contraltos as male heroic leads falls out of favor by the mid-1830s, the tenor, aided by a new chest-voice dominant style of singing, becomes the inheritor of the castrato's former role as leading man in serious Italian …
Date:
May 2018
Creator:
Ekstrum, Dave
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
Manhood in Spain: Feminine Perspectives of Masculinity in the Seventeenth Century
The question of decline in the historiography of seventeenth-century Spain originally included socio-economic analyses that determined the decline of Spain was an economic recession. Eventually, the historiographical debate shifted to include cultural elements of seventeenth-century Spanish society. Gender within the context of decline provides further insight into how the deterioration of the Spanish economy and the deterioration of Spanish political power in Europe affected Spanish self-perception. The prolific Spanish women writers, in addition, featured their points of view on manhood in their works and created a model of masculinity known as virtuous masculinity. They expected Spanish men to perform their masculine duties as protectors and providers both in public and in private. Seventeenth-century decline influenced how women viewed masculinity. Their new model of masculinity was based on ideas that male authors had developed, but went further by emphasizing men treating their wives well.
Date:
May 2016
Creator:
Gomez, Clemente, Jr.
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
Depicting Affect through Text, Music, and Gesture in Venetian Opera, c. 1640-1658
Although early Venetian operas by composers such as Claudio Monteverdi and Francesco Cavalli offer today's listeners profound moments of emotion, the complex codes of meaning connecting emotion (or affect) with music in this repertoire are different from those of later seventeenth-century operatic repertoire. The specific textual and musical markers that librettists and composers used to indicate individual emotions in these operas were historically and culturally contingent, and many scholars thus consider them to be inaccessible to listeners today. This dissertation demonstrates a new analytical framework that is designed to identify the specific combinations of elements that communicate each lifelike emotion in this repertoire. Re-establishing the codes that govern the relationship between text, musical sound, and affect in this repertoire illuminates the nuanced emotional language of operas by composers such as Claudio Monteverdi, Francesco Cavalli, Antonio Cesti, and Francesco Lucio. The new analytical framework that underlies this study derives from analysis of seventeenth-century Venetian explanations and depictions of emotional processes, which reveal a basis in their society's underlying Aristotelian philosophy. Chapters III and IV examine extant documents from opera librettists, composers, audience members, and their associates to reveal how they understood emotions to work in the mind and body. These authors, …
Date:
May 2018
Creator:
Hagen, Emily
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
With the Earth in Mind: Ecological Grief in the Contemporary American Novel
"With the Earth in Mind" responds to some of the most cutting-edge research in the field of ecocriticism, which centers on ecological loss and the grief that ensues. Ecocritics argue that ecological objects of loss abound--for instance, species are disappearing and landscapes are becoming increasingly compromised--and yet, such loss is often deemed "ungrievable." While humans regularly grieve human losses, we understand very little about how to genuinely grieve the loss of nonhuman being, natural environments, and ecological processes. My dissertation calls attention to our society's tendency to participate in superficial nature-nostalgia, rather than active and engaged environmental mourning, and ultimately activism. Herein, I investigate how an array of postwar and contemporary American novels represent a complex relationship between environmental degradation and mental illness. Literature, I suggest, is crucial to investigations of this problem because it can reveal the human consequences of ecological loss in a way that is unavailable to political, philosophical, scientific, and even psychological discourse.
Date:
May 2016
Creator:
Reis, Ashley E.
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
Russian Peasant Women's Resistance Against the State during the Antireligious Campaigns of 1928-1932
This study seeks to explore the role of peasant women in resistance to the antireligious campaigns during collectivization and analyze how the interplay of the state and resistors formed a new culture of religion in the countryside. I argue that while the state’s succeeded in controlling most of the public sphere, peasant women, engaging in subversive activities and exploiting the state’s ideology, succeeded in preserving a strong peasant adherence to religion prior to World War II. It was peasant women’s determination and adaptation that thwarted the party’s goal of nation-wide atheism.
Date:
May 2016
Creator:
Millier, Callie Anne
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
Monsters Like Us: Reexamining “Invasion of the Body Snatchers” Through the Decades
The purpose of this paper is to examine the multiple versions of "Invasion of the Body Snatchers" in concert and determine the reason for their continued presence in the American cultural landscape. To do so I will look at the novel and four films and examine the context in which they were created. In reexamining the novel and films, a central theme begins to emerge: interiority. Fear in "Invasion of the Body Snatchers" moves from an external to an internal threat. The bodily locus of the monstrous other has been re-purposed and re-projected outward. The internal nature of the monstrous threat is displayed in the narrative’s use of production and distribution, mental health professionals, pseudo-families, and the vilification of sleep. Finally, this paper will examine the studio influence on the various films and their impact on the relative endings.
Date:
May 2016
Creator:
Norton, Elizabeth Harmon
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
Food and the Master-Servant Relationship in Eighteenth and Nineteenth-Century Britain
This thesis serves to highlight the significance of food and diet in the servant problem narrative of eighteenth and nineteenth-century Britain and the role of food in master-servant relationships as a source of conflict. The study also shows how attitudes towards servant labor, wages, and perquisites resulted in food-related theft. Employers customarily provided regular meals, food, drink, or board wages and tea money to their domestic servants in addition to an annual salary, yet food and meals often resulted in contention as evidenced by contemporary criticism and increased calls for legislative wage regulation. Differing expectations of wage components, including food and other perquisites, resulted in ongoing conflict between masters and servants. Existing historical scholarship on the relationship between British domestic servants and their masters or mistresses in context of the servant problem often tends to place focus on themes of gender and sexuality. Considering the role of food as a fundamental necessity in the lives of servants provides a new approach to understanding the servant problem and reveals sources of mistrust and resentment in the master-servant relationship.
Date:
May 2017
Creator:
Weiss, Victoria A.
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
Embracing Internationalism: An Examination of Mario Lavista with an Analysis of Cinco Danzas Breves
Mario Lavista (b.1943) is widely acknowledged as one of Mexico's foremost living composers. Having acquired his music education in his native Mexico and in Europe alike, he is similar to numerous other Latin composers who were building a career in the latter half of the twentieth century. During this time, composers were relying on international aspects of avant-garde techniques, and using nationalistic Latin rhythms and melodies less. Lavista embraced internationalism, and aimed to compose works devoid of identifiable elements of nationalism. This document argues that the absence of nationalistic elements in Lavista's music has affected his notoriety outside of Mexico. The role of nationalism is assessed through a brief examination of influential Mexican composers and educators prior to 1950, followed by a discussion of education and composition in the latter half of the twentieth century. These aspects are investigated with regard to Lavista's education and resulting compositional style. A theoretical analysis of Cinco Danzas Breves para quinteto de alientos (1994) serves as a representative example of Lavista's compositional style and influence. This document aims to highlight and increase exposure of Mexican composers outside of Latin America who do not compose nationalistic music.
Date:
May 2017
Creator:
Thiemann, Amy
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
The Shrinking Opera Diva: The Impact of Sociocultural Changes upon the Casting of Women in the 20th and 21st Centuries
For most of the twentieth century, opera singers were not beholden to the ideal physical standard of women dictated by popular culture, but rather focused on serving the music and perfecting their artistry. Unprecedented sociocultural changes throughout the twentieth century exposed the shifting ideals of each generation and how they were promoted through mass media and advertising. This thesis surveys the time period of the 1890s to the present day for the purpose of analyzing cultural trends, philosophies and technologies that shaped the century. Societal pressure to make the body a project and the focus of one's own intense attention now reflects back onto the opera stage where audience members expect to see what society has dictated to be an acceptable female form. Artistic and stage directors are influenced by society's decree that only thin is beautiful, imbedding into the mindset of the art form notions that now affect how female professional opera singers are depicted and even employed.
Date:
May 2017
Creator:
McNeese, Lauren
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
Development of von Willebrand Factor Zebrafish Mutant Using CRISPR/Cas9 Mediated Genome Editing
von Willebrand factor (VWF) protein acts in the intrinsic coagulation pathway by stabilizing FVIII from proteolytic clearance and at the site of injury, by promoting the adhesion and aggregation of platelets to the exposed subendothelial wall. von Willebrand disease (VWD) results from quantitative and qualitative deficiencies in VWF protein. The variability expressivity in phenotype presentations is in partly caused by the action of modifier genes. Zebrafish has been used as hemostasis animal model. However, it has not been used to evaluate VWD. Here, we report the development of a heterozygote VWF mutant zebrafish using the genome editing CRISPR/Cas9 system to screen for modifier genes involved in VWD. We designed CRISPR oligonucleotides and inserted them into pT7-gRNa plasmid. We then prepared VWF gRNA along with the endonuclease Cas9 RNA from Cas9 plasmid. We injected these two RNAs into 1-4 cell-stage zebrafish embryos and induced a mutation in VWF exon 29 of the zebrafish with a mutagenesis rate of 16.6% (3/18 adult fish). Also, we observed a germline transmission with an efficiency rate of 5.5% (1/18 adult fish). We obtained a deletion in exon 29 which should result in truncated VWF protein.
Date:
May 2017
Creator:
Toffessi Tcheuyap, Vanina
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library
A Feminist Rereading of Selected Works by Carlos Morton
Carlos Morton is a prominent Chicano playwright that has contributed greatly to Chicano theatre, creatively and academically, since in 1970s. This thesis offers a feminist analysis of the gender representation in three of his works: Lilith (1977), La Malinche (1984), and Dreaming on a Sunday in the Alameda (1992). The female characters in these three plays possess a unique agency that allows them to challenge oppressive patriarchal standards imposed on their gender identity. The second chapter explores Morton's Lilith, a play based on a Jewish creation myth. In the play, Lilith possesses agency of her gender identity and forms a bond with Eve to fight the patriarchal gender norms used to restrict women in Chicano culture. La Malinche is an adaptation of Eurpides's Medea set in post-Conquest New Spain. Chapter three focuses on the agency displayed by La Malinche through her indigenous roots to fight for her own form of motherhood and freedom from patriarchy. The final play analyzed in this thesis is Dreaming on a Sunday in the Alameda, a dream-like play that is based on Diego Rivera's mural by the same name. Several female characters in the play demonstrate agency through their androgynous sexual identities as they unite …
Date:
May 2017
Creator:
Bruton, Rita T.
Object Type:
Thesis or Dissertation
System:
The UNT Digital Library