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Work Plan – Thematic Working Group (TWG) on Climate Change, Ozone Depletion and Ecosystem Changes (open access)

Work Plan – Thematic Working Group (TWG) on Climate Change, Ozone Depletion and Ecosystem Changes

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Date: August 2007
Creator: Thematic Working Group members on Climate Change, Ozone Depletion and Ecosystem Changes
Object Type: Text
System: The UNT Digital Library
Go Green Ontario’s Action Plan On Climate Change (open access)

Go Green Ontario’s Action Plan On Climate Change

This report outlines Ontario's Go Green Action Plan for Climate Change and its five-point action plan.
Date: August 2007
Creator: Ontario Ministry of the Environment
Object Type: Text
System: The UNT Digital Library
Regional Initiative on Environment and Health: The Third High-Level Officials’ Meeting Report (open access)

Regional Initiative on Environment and Health: The Third High-Level Officials’ Meeting Report

The Third High-Level Officials Meeting on Environment and Health in Southeast and East Asian countries was held in Bangkok, Thailand on 8 August 2007. The First High-Level Officials Meeting was held in Manila, Philippines in November 2004, where a regional initiative on environment and health was launched, and the Second High-Level Officials Meeting was convened in Bangkok, Thailand in December 2005, where a draft Charter of the Regional Forum on Environment and Health was discussed. This Third Meeting was conducted prior to the First Ministerial Meeting, to review the progress made in national and regional actions since the Second High-Level Meeting, and discuss and endorse the draft Charter and the composition and work plans of the regional Thematic Working Groups (TWGs) on six priorities for submission to the Ministerial Meeting. The agenda and annotated agenda of the meeting are given in Annexes 1 and 2, respectively, and a list of participants is attached as Annex 3.
Date: August 2007
Creator: World Health Organization
Object Type: Text
System: The UNT Digital Library
The First Ministerial Regional Forum (open access)

The First Ministerial Regional Forum

The First Ministerial Regional Forum on Environment and Health in Southeast and East Asian countries was held in Bangkok, Thailand on 9 August 2007. The forum was jointly organized by the United Nations Environment Programme (UNEP) Regional Office for Asia and the Pacific and the World Health Organization (WHO) Regional Offices for the Western Pacific and South-East Asia, and hosted by the Ministry of Natural Resources and Environment and Ministry of Public Health, Government of Thailand and the Chulabhorn Research Institute (CRI). The forum was attended by Environment and Health Ministers from fourteen Southeast and East Asian countries including Brunei Darussalam, Cambodia, People’s Republic of China, Indonesia, Japan, Republic of Korea, Lao People's Democratic Republic (PDR), Malaysia, Mongolia, Myanmar, the Philippines, Singapore, Thailand, and Viet Nam. The agenda and annotated agenda of the forum are given in Annexes 1 and 2, respectively, and a list of participants is enclosed in Annex 3.
Date: August 2007
Creator: United Nations Environment Programme
Object Type: Text
System: The UNT Digital Library
An historical and analytical survey of the Transcendental Etudes by Sergei Liapunov. (open access)

An historical and analytical survey of the Transcendental Etudes by Sergei Liapunov.

Sergei Mikhailovich Liapunov (1859-1924) was a distinguished Russian composer, pianist and teacher of the late 19th and early 20th century whose works are relatively unknown. His piano pieces were highly regarded and performed by pianists such as Konstantin Igumnov, Josef Hofmann, Josef Lhévinne, Ferruccio Busoni, and Vladimir Horowitz. However, they are rarely included in modern pianists' repertoire both in Russia and abroad, and are often viewed merely for their historic significance. Works of Liapunov are characterized by a life-affirming character and monumental beauty largely inspired by the images of nature as well as the sounds of his native Russian folk songs and dances. His music rarely conveys the urgency or profound melancholy which is often seen in the music composed during the same period by Rachmaninoff and Scriabin. Liapunov continued and enriched the great traditions of Russian music started by Glinka and The Mighty Five. He did not discover bold new ways of composing, and at the same time did not succumb to the temptation of following contemporary musical trends. The Twelve Transcendental Etudes, op. 11, dedicated to the memory of Franz Liszt, are masterpieces of immense value both from a technical and artistic standpoint. Just like Liszt's études, they …
Date: August 2007
Creator: Chernyshev, Igor
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Are Online Catalogs for Children Giving Them What They Need? Children's Cognitive Development and Information Seeking and Their Impact on Design (open access)

Are Online Catalogs for Children Giving Them What They Need? Children's Cognitive Development and Information Seeking and Their Impact on Design

Research shows children in an online environment often search by browsing, which relies heavily on recognition and content knowledge, so catalog systems for children must use effective symbols or pictorial representations, which correspond with children's own cognitive schema and level of recognition knowledge. This study was designed to look at the success of young children (ages 5 to 8) in searching 3 online public library catalogs designed for them, and it focused specifically on the pictorial representations and text descriptors used in the systems' browsing hierarchy. The research sought answer whether young children (ages 5 to 8) are really poor searchers because of cognitive development and lack of technology skills or if system design is the major reason for poor search results; i.e., Do current children's online catalog designs function in a manner that is compatible with information seeking by children? Although these results can not be generalized, this study indicates that there was a disconnect between the cognitive abilities of young users and catalog design. The study looked at search success on the 3 catalogs in relation to the catalog characteristics and individual user characteristics and makes 3 significant contributions to the field of library and information science. The …
Date: August 2007
Creator: Creel, Stacy
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Rubber Stamps and Litmus Tests: The President, the Senate, and Judicial Voting Behavior in Abortion Cases in the U.S. Federal District Courts (open access)

Rubber Stamps and Litmus Tests: The President, the Senate, and Judicial Voting Behavior in Abortion Cases in the U.S. Federal District Courts

This thesis focuses on how well indicators of judicial ideology and institutional constraints predict whether a judge will vote to increase abortion access. I develop a model that evaluates a judge's decision in an abortion case in light of ideological factors measured at the time of a judge's nomination to the bench and legal and institutional constraints at the time a judge decides a case. I analyze abortion cases from all of the U.S. Federal District Courts from 1973-2004. Unlike previous studies, which demonstrate that the president and the home state senators are the best predictors of judicial ideology, I find that the Senate Judiciary Committee at the time of the judge's nomination is the only statistically significant ideological indicator. Also, contrary to conventional wisdom, Supreme Court precedent (a legal constraint) is also a significant predictor of judicial voting behavior in abortion cases.
Date: August 2007
Creator: Craig, McKinzie
Object Type: Thesis or Dissertation
System: The UNT Digital Library
The American Trumpet Sonata in the 1950s: An Analytical and Sociohistorical Discussion of Trumpet Sonatas by George Antheil, Kent Kennan, Halsey Stevens, and Burnet Tuthill. (open access)

The American Trumpet Sonata in the 1950s: An Analytical and Sociohistorical Discussion of Trumpet Sonatas by George Antheil, Kent Kennan, Halsey Stevens, and Burnet Tuthill.

The trumpet, or some ancestral form of the trumpet, has existed nearly as long as civilization itself. Despite its long history, however, the trumpet's solo repertoire remained limited and relatively unvaried until the second half of the twentieth century. Like most music, the American trumpet sonatas from the 1950s are a reflection of the culture and history surrounding their composition. The purpose of this research is to show how the trumpet sonatas by George Antheil, Kent Kennan, Halsey Stevens, and Burnet Tuthill are both distinctly American and unmistakably from the 1950s. The post-war era in America is often viewed as a time of unbridled optimism stemming from economic prosperity and the nation's military and industrial supremacy. The decade of the 1950s is often viewed today as a simpler, happier time in America's history. The trumpet sonatas of this era reflect this primarily in their ebullient rhythms and brilliant, often heroic melodies. However, darker characteristics of the decade (the rise of communism, for example) also make veiled appearances in these four sonatas. After an overview of the social and musical trends of the decade, the central chapter of the work delineates formal, thematic, and tonal structures of each of the four …
Date: August 2007
Creator: Dearden, Jennifer Lorien
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Hardiness and public speaking anxiety: Problems and practices. (open access)

Hardiness and public speaking anxiety: Problems and practices.

This study explored the relationship between the personality construct of hardiness and public speaking anxiety. Although hardiness has been widely explored in a variety of anxiety-arousing life events, its relationship with communication anxiety had not been previously studied. Therefore, hardiness, public speaking trait anxiety, and public speaking state anxiety were measured in a course requiring an oral presentation assignment. One hundred fifty students enrolled in a basic speech communication course participated in the study. A statistically significant correlation was revealed between hardiness and trait communication anxiety. Students higher in hardiness reported lower trait communication apprehension in three contexts: 1) meeting, 2) interpersonal, and 3) group. Overall, students did not differ on measures of hardiness and a fourth communication context: public speaking anxiety. Likewise, on measures of hardiness and state public speaking anxiety, students did not differ.
Date: August 2007
Creator: Iba, Debra, L.
Object Type: Thesis or Dissertation
System: The UNT Digital Library
An Examination of the Clarinet Music of Luigi Bassi (open access)

An Examination of the Clarinet Music of Luigi Bassi

This dissertation focuses on the clarinet music of Luigi Bassi (1833-1871), an Italian clarinetist and composer. Biographical information and performance history for Luigi Bassi are included. Bassi wrote 27 works for clarinet, including 15 opera fantasies or transcriptions. Most of his works are housed in the Milan Conservatory library. This document provides analysis of all but two of Bassi's 27 works. For Bassi's pieces with ties to opera, I identified his source material and discussed the ways in which he manipulated the material. A brief synopsis of each opera is included. This study serves as a performance guide for those seeking to perform Bassi's clarinet works.
Date: August 2007
Creator: Johnson, Madeline LeBaron
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Thai Teachers' Beliefs about Learner-Centered Education: Implications for Success For Life Thailand (open access)

Thai Teachers' Beliefs about Learner-Centered Education: Implications for Success For Life Thailand

The Thai government has strongly advocated for the learner-centered education for the past decade. Success For Life Thailand (SFLT), a brain-research-based early childhood education program blended with the theories of the developmentally appropriate practices and child-centered philosophies, has been implemented in Thailand for over 8 years. The purposes of the present study were to: (a) describe the current statuses of the Thai early childhood educators' learner-centered beliefs and practices, (b) identify if the SFLT training workshop affects teachers' learner-centered beliefs and practices, and (c) examine if other variables, along with familiarity with the SFLT program, predict teachers' learner-centered beliefs and practices. Ninety-three preschool and kindergarten teachers participated in the study. Among them, 17 were SFLT trainees in 1999 and 2000 (i.e., the previously trained group), 43 were trained in Year 2006 (the currently trained group), and the others were comparable to the currently trained group by matching the key personal and school variables. The Teachers Beliefs and Practices Survey: 3-5 Year Olds (Burts et al., 2000) and the Learner-Centered Education: the Assessment of Learner- Centered (ALCP) for K-3 (McCombs, 2001) were used to collect data on the various domains of the learner-centered beliefs and practices. Findings reveal that: (a) Thai …
Date: August 2007
Creator: Israsena, Vasinee
Object Type: Thesis or Dissertation
System: The UNT Digital Library
In-between Music: The Musical Creation of Cholo Identity in Cochabamba, Bolivia (open access)

In-between Music: The Musical Creation of Cholo Identity in Cochabamba, Bolivia

Music and identity are inextricably linked. While a particular social or ethnic group's music may reflect characteristics of that group, it also functions in creating the identity of the group. In Andean Bolivia, the choloethnic group has very subjective and constantly changing boundaries. Cholo-ness is made possible through mediated cultural performances of all types, in which members actively choose elements from both criollo and Indian cultures. Music is one particularly effective way in which cholos create and maintain their identity. This thesis focuses on the ways in which cholos use music to create a hybrid identity in and around Cochabamba, Bolivia.
Date: August 2007
Creator: Jones, Eric
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Snow Spell: An Interactive Composition for Erhu, Flute, Piano, Cello, and Max/MSP (open access)

Snow Spell: An Interactive Composition for Erhu, Flute, Piano, Cello, and Max/MSP

Snow Spell is an interactive composition for erhu, flute, cello, piano, and Max/MSP interactive computer music system. This one-movement piece, Snow Spell, is intended to depict the beauty of a snow scene by presenting four different impressions of snow envisioned by the composer through music. The definition, history, and significance of interactive music are explored. Various modes of interactivity to control signal processing modules, and technical considerations for signal routing and level control in the interactive computer music system are also explored. Chinese music elements in Snow Spell including pentatonic scales, glissandi, and quotations from the Chinese folk tune River of Sorrow are investigated.
Date: August 2007
Creator: Cheng, Chien-Wen
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Evaluating Tree Seedling Survival and Growth in a Bottomland Old-field Site: Implications for Ecological Restoration (open access)

Evaluating Tree Seedling Survival and Growth in a Bottomland Old-field Site: Implications for Ecological Restoration

In order to assess the enhancement of seedling survival and growth during drought conditions, five-hundred bare-root seedlings each of Shumard oak (Quercus shumardii Buckl.) and green ash (Fraxinus pennsylvanica Marsh.) were planted each with four soil amendments at a Wildlife Management Area in Lewisville, Texas. The treatments were a mycorrhizal inoculant, mulch fabric, and two superabsorbent gels (TerraSorb® and DRiWATER®). Survival and growth measurements were assessed periodically for two years. Research was conducted on vegetation, soil, and site history for baseline data. Both superabsorbent gels gave significant results for Shumard oak survival, and one increased green ash diameter. For overall growth, significant results were found among DRiWATER®, mycorrhizae, and mulch treatments.
Date: August 2007
Creator: Boe, Brian Jeffrey
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Use of Phillips's five level training evaluation and ROI framework in the U.S. nonprofit sector. (open access)

Use of Phillips's five level training evaluation and ROI framework in the U.S. nonprofit sector.

This study examined training evaluation practices in U.S. nonprofit sector organizations. It offered a framework for evaluating employee training in the nonprofit sector and suggested solutions to overcome the barriers to evaluation. A mail survey was sent to 879 individuals who were members of, or had expressed an interest in, the American Society for Training and Development. The membership list consisted of individuals who indicated association/nonprofit or interfaith as an area of interest. Data from the survey show that training in the nonprofit sector is evaluated primarily at Level 1 (reaction) and Level 2 (learning). It also shows decreasing use from Level 3 (application) through Level 5 (ROI). Reaction questionnaires are the primary method for collecting Level 1 data. Facilitator assessment and self-assessment were listed as the primary method for evaluating Level 2. A significant mean rank difference was found between Level 2 (learning) and the existence of an evaluation policy. Spearman rho correlation revealed a statistically significant relationship between Level 4 (results) and the reasons training programs are offered. The Kruskal-Wallis H test revealed a statistically significant mean rank difference between "academic preparation" of managers with Level 3 evaluation. The Mann-Whitney U test was used post hoc and revealed …
Date: August 2007
Creator: Brewer, Travis K.
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Psychological benefits of sport participation and physical activity for adolescent females. (open access)

Psychological benefits of sport participation and physical activity for adolescent females.

Recent research has suggested that the effects of sport on well-being are mediated by psychological characteristics such as physical self-concept, instrumentality and positive body images; in addition, sport was found to be related to these psychological benefits for high school girls. However, physical self-concept played a central role by mediating the sport -body image and sport instrumentality relationships. Positive body image and instrumentality, in turn, predicted greater psychological well-being. The purpose of this investigation was to replicate earlier studies, and to examine these relationships with non-sport physical activity. Sport and physical activity were expected to contribute to higher physical self-concept, which in turn, would contribute positively to instrumentality and body image. Further, instrumentality and body image would be positively related to psychological well-being. Participants were 355 9th (n = 170) and 10th (n = 193) graders and they completed measures of involvement in sport/physical activities, physical self-concept, instrumentality, body satisfaction, self-esteem, satisfaction with life, depression, and demographics. Structural equation modeling was utilized to analyze the data. Overall, for both sport and physical activity, the models fit the data well (sport model: NNFI=.95, CFI=.96, SRMR=.08, RMSEA=.09, physical activity model: NNFI=.96, CFI=.97, SRMR=.08, RMSEA=.09). Specifically, sport participation was positively related to physical …
Date: August 2007
Creator: Boyer, Elizabeth M.
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Fractional Brownian motion and dynamic approach to complexity. (open access)

Fractional Brownian motion and dynamic approach to complexity.

The dynamic approach to fractional Brownian motion (FBM) establishes a link between non-Poisson renewal process with abrupt jumps resetting to zero the system's memory and correlated dynamic processes, whose individual trajectories keep a non-vanishing memory of their past time evolution. It is well known that the recrossing times of the origin by an ordinary 1D diffusion trajectory generates a distribution of time distances between two consecutive origin recrossing times with an inverse power law with index m=1.5. However, with theoretical and numerical arguments, it is proved that this is the special case of a more general condition, insofar as the recrossing times produced by the dynamic FBM generates process with m=2-H. Later, the model of ballistic deposition is studied, which is as a simple way to establish cooperation among the columns of a growing surface, to show that cooperation generates memory properties and, at same time, non-Poisson renewal events. Finally, the connection between trajectory and density memory is discussed, showing that the trajectory memory does not necessarily yields density memory, and density memory might be compatible with the existence of abrupt jumps resetting to zero the system's memory.
Date: August 2007
Creator: Cakir, Rasit
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Politics and the American clergy: Sincere shepherds or strategic saints? (open access)

Politics and the American clergy: Sincere shepherds or strategic saints?

Scholars have evaluated the causes of clergy political preferences and behavior for decades. As with party ID in the study of mass behavior, personal ideological preferences have been the relevant clergy literature's dominant behavioral predictor. Yet to the extent that clergy operate in bounded and specialized institutions, it is possible that much of the clergy political puzzle can be more effectively solved by recognizing these elites as institutionally-situated actors, with their preferences and behaviors influenced by the institutional groups with which they interact. I argue that institutional reference groups help to determine clergy political preferences and behavior. Drawing on three theories derived from neo-institutionalism, I assess reference group influence on clergy in two mainline Protestant denominations-the Presbyterian Church (USA) and the Episcopal Church, USA. In addition to their wider and more traditional socializing influence, reference groups in close proximity to clergy induce them to behave strategically-in ways that are contrary to their sincerely held political preferences. These proximate reference groups comprise mainly parishioners, suggesting that clergy political behavior, which is often believed to affect laity political engagement, may be predicated on clergy anticipation of potentially unfavorable reactions from their followers. The results show a set of political elites (the clergy) …
Date: August 2007
Creator: Calfano, Brian Robert
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Toward a systematic evaluation of evaluating favorable conditions in a parent training program: The pursuit of happiness. (open access)

Toward a systematic evaluation of evaluating favorable conditions in a parent training program: The pursuit of happiness.

Research has shown that parents of children with disabilities, such as autism, experience significantly higher stress levels than parents of typically developing children. It has been suggested that parent education programs, in particular naturalistic communication training, will reduce parental stress. Most of the literature in this area has relied on parental reports and has only focused on decreasing stress and has not directly addressed increasing alternate feelings, such as happiness. In different but related areas of behavior analysis, an emphasis has been placed on the importance of happiness as a quality of life indicator and that the development of multileveled assessment is sorely needed. This study was designed to analyze one set of measures within a data-based intervention program for parents of toddlers with autism. The Family Connections Project (FCP) is a parent training project designed to enhance the quality of relationships for families who have toddlers with autism. Within this project parents are taught to identify and arrange opportunities to interact with their children in ways that will increase motivation and social responsivity. This study looked at the collateral effects of this training program and investigated if FCP affected the relationship between parents and their toddlers; of particular interest …
Date: August 2007
Creator: Broome, Jessica L.
Object Type: Thesis or Dissertation
System: The UNT Digital Library
The Impact of Ownership, Regulation Issues and Technology Adoption on the Introduction of Digital Terrestrial Television: A Comparison of the United States and Mainland China (open access)

The Impact of Ownership, Regulation Issues and Technology Adoption on the Introduction of Digital Terrestrial Television: A Comparison of the United States and Mainland China

This study compares the impact of media ownership, regulation and policy, and technology adoption on the introduction of digital terrestrial television in the United States and Mainland China. Through the use of a case study approach, a qualitative and quantitative examination is given. The results indicate that private group ownership throughout the U.S. digital terrestrial television industry and state ownership in China's television industry lead to the different paths to digital transition. Both governments, however, are deeply involved in respective digital initiatives and play an important role in the implementation from analog to digital. The technical standard adoption in the two countries places the underpinning for the future development of digital television (DTV), which also results in China lagging behind the United States by almost ten years. The differences of technological environments in households and income among consumers in the two countries further predict the intention to DTV adoption.
Date: August 2007
Creator: Guo, Miao
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Wealth and Regime Formation: Social and Economic Origins of the Change Toward Democracy (open access)

Wealth and Regime Formation: Social and Economic Origins of the Change Toward Democracy

This study explores the relationship between economic development, social mobility, elites, and regime formation. I argue that the genesis of regime formation, in general, and of democratic regimes, in particular, is determined by the type of economic structure a society possesses, on the one hand, and on the degree the to which demands from disfranchised groups do or do not pose a substantial threat to the interests of elites who occupy the upper strata of the social and economic status hierarchy. Second I demonstrate that the dynamics of transition to wider political participation, as the core element of a democratic system of governance, and the survival of such change are different. In what follows I illustrate that some factors that have been found to dampen the chances for wider participation or have been found to be unrelated to onset of a democratic system of governance have considerable impacts on the durability of the democratic regimes. In a nutshell, the analysis points to the positive effects of mineral wealth and income inequality on the prospects of a democratic survival. Using a cross-national time series data set for all countries for the period between 1960 and 1999 I put the hypotheses to …
Date: August 2007
Creator: Gurses, Mehmet
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Supply Chain Network Evolution: Demand-based Drivers of Interfirm Governance Evolution (open access)

Supply Chain Network Evolution: Demand-based Drivers of Interfirm Governance Evolution

Which form of exchange governance performs better in a dynamic environment? This remains an unanswered question in the transaction cost analysis (TCA) and relational exchange literatures. Some researchers purport that transactional governance provides superior performance by providing firms the flexibility to change suppliers. Others suggest that relational governance leads to superior performance because of the willingness of both parties to adapt. Reviews of TCA have turned up ambivalent empirical findings with regard to the effects of uncertainty despite a track record of strong empirical support for other predictions. Because most of TCA and relational exchange theories' predictions enjoy strong support, this research builds upon these theories to propose a theoretical modeling framework for a dynamic environment in a supply chain network (SCN) setting. This dissertation extends TCA and relational exchange to a dynamic, network environment. It uses the approach of building a simulation in order to study in detail the relationship between key exchange factors and the selection of transactional and relational exchange governance over time. This research effort extended TCA theory with a complex adaptive model of supply chain network governance evolution that attempts to link environmental, network, production, firm and exchange factors in a continuously evolving loop. The …
Date: August 2007
Creator: Gravier, Michael J.
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Detecting Masculinity: The Positive Masculine Qualities of Fictional Detectives. (open access)

Detecting Masculinity: The Positive Masculine Qualities of Fictional Detectives.

Detective fiction highlights those qualities of masculinity that are most valuable to a contemporary culture. In mysteries a cultural context is more thoroughly revealed than in any other genre of literature. Through the crimes, an audience can understand not only the fears of a particular society but also the level of calumny that society assigns to a crime. As each generation has needed a particular set of qualities in its defense, so the detective has provided them. Through the detective's response to particular crimes, the reader can learn the delineation of forgivable and unforgivable acts. These detectives illustrate positive masculinity, proving that fiction has more uses than mere entertainment. In this paper, I trace four detectives, each from a different era. Sherlock Holmes lives to solve problems. His primary function is to solve a riddle. Lord Peter Wimsey takes on the moral question of why anyone should detect at all. His stories involve the difficulty of justifying putting oneself in the morally superior position of judge. The Mike Hammer stories treat the difficulty of dealing with criminals who use the law to protect themselves. They have perverted the protections of society, and Hammer must find a way to bring them …
Date: August 2007
Creator: Griswold, Amy Herring
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Fluorescence labeling and computational analysis of the strut of myosin's 50 kDa cleft. (open access)

Fluorescence labeling and computational analysis of the strut of myosin's 50 kDa cleft.

In order to understand the structural changes in myosin S1, fluorescence polarization and computational dynamics simulations were used. Dynamics simulations on the S1 motor domain indicated that significant flexibility was present throughout the molecular model. The constrained opening versus closing of the 50 kDa cleft appeared to induce opposite directions of movement in the lever arm. A sequence called the "strut" which traverses the 50 kDa cleft and may play an important role in positioning the actomyosin binding interface during actin binding is thought to be intimately linked to distant structural changes in the myosin's nucleotide cleft and neck regions. To study the dynamics of the strut region, a method of fluorescent labeling of the strut was discovered using the dye CY3. CY3 served as a hydrophobic tag for purification by hydrophobic interaction chromatography which enabled the separation of labeled and unlabeled species of S1 including a fraction labeled specifically at the strut sequence. The high specificity of labeling was verified by proteolytic digestions, gel electrophoresis, and mass spectroscopy. Analysis of the labeled S1 by collisional quenching, fluorescence polarization, and actin-activated ATPase activity were consistent with predictions from structural models of the probe's location. Although the fluorescent intensity of the …
Date: August 2007
Creator: Gawalapu, Ravi Kumar
Object Type: Thesis or Dissertation
System: The UNT Digital Library