The purpose of this project is to produce a complete and thorough notebook that may be used to supplement laboratory coursework. Its intent is to be used primarily by the students to aid them in understanding background information and the proper laboratory procedures involved in various types of experiments. The laboratory notebook is a summation of all the experiments and procedures used in the six-credit hour Advanced Microbial and Molecular Biology (BIOL 5160) course offered during the summer semester at the University of North Texas. This class is a team taught effort by Professors O'Donovan and Kunz. The course is constructed as an intensive practice exercise to teach the student about gene mutations, biosynthetic pathways, preparation and analysis of plasmid DNA, and many other topics included in the notebook.

Canonical correspondence analysis (CCA) was used to explore relationships between measured environmental variables and in situ phytoplankton communities in a moderately eutrophic North Texas Reservoir.

The flagellum of Trypanosoma brucei contains a family of antigenically related EF-hand calcium-binding proteins which are called the calflagins. Genomic Southern blots indicated that multiple copies of calflagin genes occur in T brucei. All of the copies were contained in a single 23 kb Xhol-Xhol fragment. Genomic fragments of 2.5 and 1.7 kb were cloned that encoded calflagin sequences. Two new members of the calflagin family were found from genomic clone sequences. The deduced amino acid sequences of the genomic clones showed the calflagin genes were arranged tandemly along the genomic fragments and were similar to previously described calflagins. The calflagin genes were related by two unrelated 3' flanking sequences. An open reading frame that was unrelated to any calflagin was found at the 5' end of the 2.5 kb genomic fragment. Each encoded protein (~24,000u) contained three EF-hand calcium-binding motifs and one degenerate EF-hand motif. In general, variability among the T. brucei calflagins is greater than related proteins in T. lewisii and T. cruzi. This variability results from amino acid substitutions at the amino and carboxy termini, and duplication of internal segments.

Thermoplastic polyimides (TPIs) exhibit high glass transition temperatures (Tgs), which make them useful in high performance applications. Amorphous and semicrystalline TPIs show sub-Tg relaxations, which can aid in improving strength characteristics through energy absorption. The a relaxation of both types of TPIs indicates a cooperative nature. The semicrystalline TPI shows thermo-irreversible cold crystallization phenomenon. The polymer liquid crystal (PLC) used in the blends is thermotropic and with longitudinal molecular structure. The small heat capacity change (ACP) associated with the glass transition indicates the PLC to be rigid rod in nature. The PLC shows a small endotherm associated with the melting. The addition of PLC to the semicrystalline TPI does not significantly affect the Tg or the melting point (Tm). The cold crystallization temperature (Tc) increases with the addition of the PLC, indicating channeling phenomenon. The addition of PLC also causes a negative deviation of the ACP, which is another evidence for channeling. The TPI, PLC and their blends show high thermal stability. The semicrystalline TPI absorbs moisture; this effect decreases with the addition of the PLC. The absorbed moisture does not show any effect on the degradation. The addition of PLC beyond 30 wt.% does not result in an improvement …

This dissertation approaches the work of South African/Botswanan novelist Bessie Head, especially the novel A Question of Power, as positioned within the critical framework of the postcolonial paradigm, the genius of which accommodates both African and African American literature without recourse to racial essentialism. A central problematic of postcolonial literary criticism is the ideological stance postcolonial authors adopt with respect to the ideology of the metropolis, whether on the one hand the stances they adopt are collusive, or on the other oppositional. A key contested concept is that of universality, which has been widely regarded as a witting or unwitting tool of the metropolis, having the effect of denigrating the colonial subject. It is my thesis that Bessie Head, neither entirely collusive nor oppositional, advocates an Africanist universality that paradoxically eliminates the bias implicit in metropolitan universality.

The purpose of this study was to demonstrate the feasibility of developing an art curriculum in Qatar, using the principles of the DBAE curriculum in conjunction with technology. Many of the challenges facing art educators and the art curriculum in Qatar can be approached through the multimedia applications of DBAE, which will provide instructors and students with an opportunity to more readily interact with visual art and to discover its educational relevance. Additionally, this study attempted to discover whether teachers are engaged in implementing technology in the art classroom and whether they are given the opportunity to engage in art to their satisfaction.

The purpose of this study was to determine whether there were differences in the levels of awareness and perception concerning distance education among the leadership at the seven campuses of the Texas State Technical College (TSTC) System.

This paper discusses the concept of figurative formalism and its application in six paintings on canvas by the author. Linda Leonhart McCall explores the work of Johannes Vermeer, Edward Hopper, and Richard Diebenkorn in discussing abstraction and representation.

A behavioral model for acute responses in bivalves, was developed using time series analysis for use in a real-time biomonitoring unit. Stressed bivalves closed their shell and waited for the stressful conditions to pass. Baseline data showed that group behavior of fifteen bivalves was periodic, however, individuals behaved independently. Group behavior did not change over a period of 20 minutes more than 30 percent, however, following toxic exposures the group behavior changed by more than 30 percent within 20 minutes. Behavior was mathematically modeled using autoregression to compare current and past behavior. A logical alarm applied to the behavior model determined when organisms were stressed. The ability to disseminate data collected in real time via the Internet was demonstrated.

Utilization of cyanide as a nutritional nitrogen source in P. fluorescens NCIMB 11764 was shown to involve a novel metabolic mechanism involving nonenzymatic neutralization outside of cells prior to further enzymatic oxidation within. Several cyanide degrading enzymes were produced by NCIMB 11764 in response to growth or exposure to cyanide, but only one of these cyanide, oxygenase (CNO), was shown to be physiologically required for assimilation of cyanide as a growth substrate.

The focus of the problem was to challenge the more traditional. Western approaches to jewelry as adornment in respect to areas such as placement and scale. Approaching adornment as sculptural forms interacting with the human body could possibly challenge the individual's awareness of jewelry as wearable art. This approach brought up the issue of using the human body as a pedestal for adornment.

This dissertation proposes that the Sonatine broadly unfolds a kinetic structure that stems from the traditional tension-relief model and, consequently, its dependence on tradition proves much deeper than Boulez would acknowledge.

A life history study of Callibaetis floridanus was conducted over the wet cycle of a playa on the Southern High Plains of Texas from June through September 1995.

Bacterial aspartate transcarbamoylases (ATCase's) are divided into three classes that correspond to taxonomic relationships within the bacteria. The opportunistic pathogen Moraxeila catarrhalis has undergone several reclassifications based on traditional microbiological criteria. The previously uncharacterized ATCase from M. catarrhalis was purified to homogeneity and its chemical properties characterized. The ATCase from M. catarrhalis is a class C ATCase with an apparent molecular mass of 480-520 kDa. The M. catarrhalis ATCase is a dodecomer composed of six 35 kDa polypeptides and six 45 kDa polypeptides. The enzyme has an unusually high pH optimum of greater than pH 10. The enzyme exhibited hyperbolic kinetic with a Km for aspartate of 2 mM. A single, separate 78 kDa dihydroorotase from M. catarrhalis was identified and it was not associated with ATCase. These data support the reclassification of M. catarrhalis out of the Neisseriaceae family.

Standard psychological assessment instruments have not produced consistent results by which decisions can be made regarding the appropriate placement and legal disposition of an individual who has committed a sexual offense. The purpose of the present study was to systematically investigate deception and dissimulation as measured by three assessment instruments commonly utilized with sex offenders. A denial classification system was utilized in order to classify offenders into categories based on their level of admission to the legal system. The four group classification system did not produce significant differences on all measures of deception and dissimulation. Contrary to previous research, admitters were found to respond more defensively than deniers on one of the assessment instruments. In addition, partial deniers were identified as responding significantly differently from both admitters and deniers on a separate instrument. The differences found suggest that sex offenders' level of deception is multifaceted. Difficulties in identifying classificatory strategies and implications for theoretical conceptions of denial within this population are discussed.

The purposes of this study were (a) to analyze whether any significant differences exist in students' satisfaction among the 11 composite scales/satisfaction measures of the SSI (retention programs); (b) to determine whether significant differences exist in satisfaction among students of the institution based on their demographic characteristics of gender, age, ethnicity, class load, and employment; and (c) to record findings, draw conclusions, and make recommendations from the study. The research was conducted using a questionnaire, The Student Satisfaction Inventory (SSI), developed by Juillreat and Schreiner in 1994. The instrument measures, among other matters, students' perceptions and satisfaction. The population of the study comprised all students at the institution during the 1996-1997 school year. A total of 312 students was sampled, with 182 (58%) returns received. Statistical treatments used to analyze the collected data included frequencies, percentages, means, standard deviation, multiple analysis of variances (MANOVA), one-way analysis of variance (ANOVA), and Tukey's Post Hoc t-test for multiple comparison.

The purpose of this study was to compare the self-efficacy of two groups of first year teachers working in a large urban school district in North Texas. Twenty-eight of the participants were certified teachers. Ten participants held college degrees unrelated to teaching and were undergoing an alternative certification process. The Teacher Efficacy Scale was administered at the beginning and the end of the school year. Data from this scale was analyzed to determine if there were differences between the regular certification teachers and the alternative certification teachers at the beginning and the end of the school year, and to determine if their sense of efficacy changed over the course of the school year.

A study was undertaken to explore relationships between wetland characteristics which make them efficient water purifiers versus their ability to serve as wildlife habitat. The effects of designing constructed wetlands for improved habitat on water treatment efficiencies were quantified. Results indicate that some sacrifice in treatment efficiency is required and that the degree of efficiency reduction is dependant upon pollutant loading rates. However, sacrifice in efficiency is much smaller than increase in habitat quality, and can be offset by increasing wetland area. A practical, theoretical application was then attempted.

Recently, multi-source feedback has been a popular way of providing performance-related feedback to individuals in many organizations. Many who use multi-source feedback consider Rating Convergence, others seeing target individuals as they see themselves, to be a positive outcome of this process. However, the variables that account for Rating Convergence are not known. This study investigated whether the personality factor Extroversion and Behavioral Consistency, acting as a moderator variable, could account for Self-other Rating Convergence, particularly the Convergence between self and peer Ratings. The sample consisted of 235 mid-level managers from a variety of industries who were participants in individual career development workshops. Using structural equation modeling, the results indicated that a model consisting of a single Extroversion factor could account for the convergence of self-peer ratings. This finding calls into question the significance of Rating Convergence when using multi-source rating instruments that provide feedback on trait characteristics since it may be heavily influenced by a single personality factor rather than observers' comprehensive understanding of the ratee's performance.

A data acquisition system was set up to measure gas temperatures and pressures at various points on a liquid-nitrogen-powered vehicle. The experiment was attempted to develop a data acquisition method for applications on engines that use liquid air as the fuel. Two thermocouples and a pressure transducer were connected using data acquisition instruments interfaced to a laptop computer to acquire data.

Deserts! Have Known contains a scholarly preface exploring why writers write, examining the characteristics offictionwriters, and addressing the importance of place, both emotional and geographical, in fiction. Four original short stories are included in this thesis. "Miracle at Mita" depicts an aging surfer trying to overcome his fear of commitment. "Coyote Man" explores a father's guilt and the isolation resulting from that guilt. "Time, and Time Again" traces a young woman's fear of marriage to her memory of her parents' relationship, and "Paraplegia" examines a young woman immobilized by her own lack of self-esteem. These stories are connected through their themes of isolation and reconnection.

This thesis analyzes the destruction of imagery dedicated to Saint Thomas Becket in order to investigate the nature of sixteenth-century iconoclasm in Reformation England. In doing so, it also considers the veneration of images during the late Middle Ages and the Renaissance. Research involved examining medieval and sixteenth-century historical studies concerning Becket's life and cult, anti-Becket sentiment prior to the sixteenth century, and the political circumstances in England that led to the destruction of shrines and imagery. This study provides insight into the ways in which religious images could carry multifaceted, ideological significance that represented diversified ideas for varying social strata--royal, ecclesiastical and lay.

With numerous bioassessment methodologies available, a regional protocol needs to be developed to ensure that results are comparable. A regional assessment protocol was developed that includes collecting five benthic macroinvertebrate samples, identifying organisms to genus, and calculating the following metrics: Number of Taxa, Total Number of Individuals, Simpson's Diversity Index, Shannon's Diversity Index, Percent Contribution of Dominant Taxa, Hilsenhoffs Biotic Index, and Percent Contribution of Dipterans. Once the protocol was developed, it was used to assess the Bayou Chico tributaries and watershed. All three tributaries had been significantly impacted by human activity as had the watershed as a whole. This study indicates that a regional protocol could be developed and is appropriate for biomonitoring at the watershed scale.

This study views the media as a powerful agent which constructs the narratives of political candidates. In order to determine whether the media constructs the narratives of male and female political candidates differently, newspaper articles were analyzed for two 1994 Congressional races, each involving a male and a female candidate (Thurman versus Garlits and Byrne versus Davis). The first research question posed the following question: Does the media devote more coverage to male or female candidates? The next question concerned media endorsements of the candidates. Third, the settings in which the media portrayed the male and female candidates were compared. Finally, differences in the media's attitude toward male and female candidates were analyzed.