Pre-service teachers are entering the field as novice practitioners with concerns regarding their ability to confidently and effectively implement key components of response to intervention (RTI). This concurrent mixed-methods study explores pre-service teachers' (N =169) perceptions and experiences with key components of RTI (e.g., screening, multi-tiered evidence-based intervention, progress monitoring, and data-based decision making). A questionnaire in conjunction with open-response items and four focus groups provided data to identify aspects of university coursework and field experiences that contribute to pre-service teachers' perceived ability to confidently implement key components of RTI. The results of this investigation show between group differences in perception and experiences related to RTI. Special education certification seekers reported higher perceived confidence, receiving more coursework, and having more field experiences with RTI than elementary, middle grade, and secondary certification seekers. Among all groups, secondary certification seekers reported the lowest confidence, least amount of coursework, and fewest field experiences with RTI. Pre-service teachers in this study valued coursework and knowledgeable instructors who emphasized the components of RTI and participating in hands-on class activities. Participants noted benefits from or a desire for field experiences with struggling learners and having mentors with knowledge and expertise in RTI. Study findings suggest providing …