Relationship of Estrous Cycle to Herpes Simplex Virus Type 2 Susceptibility in Female Mice (open access)

Relationship of Estrous Cycle to Herpes Simplex Virus Type 2 Susceptibility in Female Mice

In CBA/NJ mice, splenic natural killer (NK) cell activity varies with stages of estrous. Susceptibility of ICR mice to intravaginal inoculation of herpes simplex virus type 2 (HSV-2) decreases with age. Susceptibility of female ICR and CBA/NJ mice to HSV-2 inoculated intravaginally and intraperitoneally was examined during the estrous cycle. In cycling ICR mice, greatest susceptibility to intravaginal inoculation was observed during diestrous and the least during metestrous. CBA/NJ mice were most susceptible to intravaginal inoculation of HSV-2 during diestrous. ICR mice were ovariectomized to mimic diestrous and found to be highly susceptible to intravaginal inoculation at all virus doses. No difference in susceptibility among phases of the estrous cycle was seen following intraperitoneal inoculation.
Date: August 1987
Creator: Teepe, Annette
System: The UNT Digital Library
Effects of Gold Sodium Thiomalate on Murine Spleen Cells (open access)

Effects of Gold Sodium Thiomalate on Murine Spleen Cells

The effects of gold sodium thiomalate (GST) on murine spleen cells were investigated using in vitro mitogen blastogenesis techniques. Addition of GST to intact spleen cells resulted in a decreased blastogenic response to the T cell mitogen, concanavalin A (Con A). Thymidine uptake of spleen cells depleted of macrophages and cultured with Con A and GST demonstrated biphasic effects. At 2.5 pg Con A/ml, blastogenesis of macrophage depleted spleen cells was inhibited to a lesser degree than intact spleen cells; whereas, at 0.5 pg Con A/ml, the macrophage depleted spleen cells were inhibited to a greater degree than the intact spleen cells. Addition of GST at intervals ranging from 0 to 48 hours indicated that inhibition occurred within 36 hours following mitogen stimulation. These results suggest that GST inhibits early events of lymphocyte activation by direct interaction with lymphocytes.
Date: December 1986
Creator: Brownback, Paul (Paul Eldon)
System: The UNT Digital Library
Gold Compounds and Rheumatoid Arthritis Murine Studies of the Immune Response to Gold Sodium Thiomalate (open access)

Gold Compounds and Rheumatoid Arthritis Murine Studies of the Immune Response to Gold Sodium Thiomalate

Balb/c normal mice were used to study the effects of gold sodium thiomalate (GST) on intact, nonadherent, and adherent mononuclear spleen cells. The three populations were tested for the following aspects: in vitro effects of GST on the mitogen-triggered DNA synthesis; intracellular levels of cyclic AMP; and chemotaxis ability. These studies showed that GST inhibited the proliferative responses of all three populations as the concentration of GST increased. Cyclic AMP levels in the nonadherent population increased as the GST concentration increased. GST had a biphasic effect on the adherent population. At concentrations of 5 and 10 jag/ml, GST suppressed the cyclic AMP levels, and at concentration of 50 pg/ml it enhanced the cyclic AMP levels. GST had no effect on the cyclic AMP levels in the intact mononuclear spleen cells. GST appeared to have an inhibitory effect on the chemotaxis ability of all three populations of spleen cells.
Date: August 1986
Creator: Sayahtaheri, Sousan
System: The UNT Digital Library
Internal Radiolabeling of Mycobacterial Antigens and Use in Macrophage Processing Studies (open access)

Internal Radiolabeling of Mycobacterial Antigens and Use in Macrophage Processing Studies

Mycobacter avium complex serovars 4 and 20 were cultured in the presence of [3H] fucose, [3H]-methionine, and [3H]-mannose to specifically radiolabel the oligosaccharide of the glycopeptidolipid (GPL) antigens. Distribution of radioactivity in lipid was determined by thin-layer chromatographic methods. Examination of acid hydrolysates from radiolabeled antigens revealed that [3H]-methionine incorporated into methylated sugars in polar and apolar GPL components, whereas [3H]-mannose incorporated exclusively into the oligosaccharide of polar GPL antigens. Least incorporation of radiolabel into antigens was observed with [3H]-fucose. Use of radiolabeled serovar 4 antigens in macrophage uptake studies revealed maximum uptake to be slightly above 250 gg/ 3.2 x 105 cells. Timed experiments demonstrated that GPL antigens were relatively inert to degradation by resident peritoneal macrophages.
Date: August 1988
Creator: Woodbury, Julie L. (Julie Lynn)
System: The UNT Digital Library
The Adenosine Antagonist Aminophylline Attenuates Pacing-Induced Coronary Functional Hyperemia (open access)

The Adenosine Antagonist Aminophylline Attenuates Pacing-Induced Coronary Functional Hyperemia

Left coronary blood flow (LCBF), left ventricular oxygen, extraction [(a-v)O₂ ], and myocardial oxygen consumption (MVO₂) were monitored in 10 dogs. HR was paced at 120 bpm and then increased to 180 bpm to elicit a hyperemic response (ΔLCBF). During the hyperemia, the vaso-dilatory response to exogenous adenosine (F_AD) was tested. Twenty min. after injection of aminophylline (100 mg/i.v.), HR was again increased. F_AD was again tested. The pacing-induced increase in MVO₂ (ΔMVO₂) was not affected by aminophylline (P>0.05). However, the slope ΔLCBF/ΔMVO₂ was decreased, and the slope (a-v)O₂ /ΔMVO₂ was increased. F_AD was also decreased and the magnitude of the reduction was correlated with the decrease in ΔLCBF/ΔMVO₂ (r=0.82). These results suggest that adenosine may play an role in coronary functional hyperemia induced by increases in heart rate.
Date: December 1984
Creator: Randall, John Robert
System: The UNT Digital Library
Alpha-Adrenergic Modulation of Coronary Blood Flow and Cardiac Function During Exercise in Dogs (open access)

Alpha-Adrenergic Modulation of Coronary Blood Flow and Cardiac Function During Exercise in Dogs

In the present study alpha-receptor modulation of coronary flow and cardiac function was examined in exercising dogs, chronically instrumented to measure: circumflex blood flow velocity (CFV), heart rate (HR), global left ventricular function (LVP and dP/dt Max) and regional left ventricular function (%SL and dL/dt (s)max). During exercise, local adrenergic blockade was produced by intracoronary injection of 1.0 mg phentolamine ( anon-specific alpha-antagonist) or .5 mp prazosin. Exercise significantly increased HR, LVP, dP/dt max, CFV, %SL and dL/dt (s)max. Neither alpha-antagonist produced changes in HR, LVP or %SL; however, both phentolamine and prazosin produced significant increses in dP/dtmax, CFV and dL/dt(s)max of the alpha-blocked region, when compared to their exercise level before alpha-blockade. It is suggested that an alpha1-adrenergic vasoconstriction limits coronary vasodilation and, thereby, cardiac function during exercise.
Date: December 1985
Creator: Overn, Steven P. (Steven Paul)
System: The UNT Digital Library