This manual has been designed for a class of twenty- four students concurrently enrolled in the lecture course. The laboratory aids in the learning process and fosters an interest in the science of genetics. This manual and the experiments contained within are both informative and fun. The manual correlates with and expands upon the genetics course. Each investigation, with the exception of the Drosophila melanogaster project, can be completed in a 3-4 hour timeframe. This manual provides a “hands on” experience of theories simply discussed in the lecture course. This manual is intended to be a one-source manual where each investigation is designed to include an adequate introduction. Special attention has been given for each investigation with both the student and instructor in mind.

The second order rate constants for nucleophilic substitution by methoxide of (Z)- and (E)-O-methylbenzohydroximoyl fluorides [C6H4C(F)=NOCH3] with various substituents on the phenyl ring [p-OCH3 (1h, 2h), p-CH3 (1g, 2g), p-Cl (1f, 2f), p-H (1e, 2e), (3,5)-bis-CF3 (1i, 2i)] in 90:10 DMSO:MeOH have been measured. A Hammett plot of these rate constants vs σ values gave positive ρ values of 2.95 (Z isomer) and 3.29 (E isomer). Comparison of these rates with methoxide substitution rates for Omethylbenzohydroximoyl bromide [C6H4C(Br)=NOCH3] and Omethylbenzohydroximoyl chloride [C6H4C(Cl)=NOCH3] reveal an element effect for the Z isomers of Br:Cl:F(1e) = 2.21:1.00:79.7 and for the E isomers of Cl:F(2e) = 1.00:18.3. With the p-OCH3-imidoyl halides the following element effects are found: Br:Cl:F(1h) = 2.78:1.00:73.1 for the Z isomer and Br:Cl:F(2h) = 1.97:1.00:12.1 for the E isomer. Measurement of activation parameters revealed ∆S≠ = -17 eu for 1e and ∆S≠ = -9.9 eu for 2e. Ab initio calculations (HF/6-31+G*, MP2/6-31+G*//HF/6-31+G*, B3LYP/6- 31+G*//HF/6-31+G*, HF-SCIPCM/6-31+G*//HF/6-31+G*) were performed to define the reaction surface. These calculations demonstrate a relatively large barrier for nucleophilic attack in relation to halogen loss and support the experimental findings that this reaction proceeds by an addition-elimination mechanism (AN# + DN). The imidoyl fluorides have been used to synthesize …

This study examines the relationship between service learning innovations and improved academics, self-concept, and social or personal growth in middle and high school students. Meta-Analysis is employed to arrive at effect-size estimates for each construct. A historical overview of service learning is presented and a detailed description of the study selection process is provided. The data revealed a moderate relationship between service learning participation and academics, self-concept and social or personal growth in middle and high school students. The findings are presented, and some appropriate conclusions are drawn. A discussion of the implications of these findings and recommendations for future research are also provided.

The first part of this paper describes the Meissner effect in superconductors which serves as the most definitive evidence for superconductivity. It is shown that the microwave perturbation technique may be used to demonstrate this effect. By measuring the changes of resonant frequency and inverse quality factor Q of a microwave cavity with a small volume of sample loading, the Meissner effect can be shown by using the Slater perturbation equation. The experimental system is described with details and the basic principle of each component discussed. The second part of this work describes the technique employed to do the actual measurements. The experiments were conducted on samples of Gallium Arsenide (GaAs) and lead zirconate titanate (PZT) to look for the possible high temperature superconductivity properties. Results of these experiments are presented and discussed. Conclusion and suggestions to future exploration are made.

A potential alternative to acrylonitrile-butadiene-styrene (ABS) and polycarbonate+ABS (PC+ABS), pigmented mineral-filled polypropylene (PP) finds an opening in automotive interior components such as instrument panels, knee bolsters, consoles, etc. Because of the lack of surface aesthetics, pigmented mineral-filled PP is experiencing a limitation to its acceptance in many applications. This study focuses on exploring various mineral fillers and additives in polypropylene to provide a material with enhanced scratch resistance. Several physical properties including Rockwell and Shore D hardness are investigated, and it is determined that Filler W improves scratch resistance. It is also determined that Filler T-filled-PP has poor scratch resistance even with the addition of a lubricant.

The study sought to determine the relationships of preference responses to grade, gender, familiarity, musical training, peers'/parents' listening habits, music media use, and listening contexts. Grade six through nine Hong Kong students (N = 310) completed the audio preference test followed by verbal responses to training, peers'/parents' preferences, leisure/music media involvement, and listening context. Results indicated: The preferred genres, in descending order, were Western pop/rock, Cantopop/rock, Western classical; the disliked genres were jazz, Chinese, and non- Western/non-Chinese. Preference correlated strongly with genre familiarity. Pop genres were the most familiar to all adolescents. The students' preference toward Western pop/rock and Cantopop/rock associated with several listening contexts: solitary listening, having great freedom to choose one's desired music for listening, listening to music in one's room, and listening to music as background activity. The adolescents expressed that their leisure activities were spent with their family and friends. However, they made it clear that music listening was a personal activity that very likely was listened to alone. On all listening occasions, the girls exhibited a more positive response than the boys did. With four to five hours daily leisure time, the adolescents watched TV for three to four hours while spending less than two …

Estrogen is the natural agonist of the estrogen receptor (ER). However, certain plant-derived compounds or phytoestrogens have been identified that mimic estrogens and act as agonists and/or antagonists of ERs, depending on subtype and target tissue. Understanding how phytoestrogens interact with ERs, and therefore effect the estrogenic response, may prove beneficial in hormone replacement therapy and in the prevention and treatment of hormone-related diseases. Using Thin Layer Chromatography, gas chromatography/mass spectrometry (GC/MS), and proton nuclear nagnetic resonance (HNMR), I identified 4-ethoxymethylphenol (4EM) found in Maclura pomifera. While most phytoestrogens are heterocyclic compounds, 4EM is a simple phenol that acts as an agonist of ER-alpha and -beta in HeLa and MCF-7 cells. To study the effect of 4EM on ER-alpha and -beta activity, I performed transient transfection assays and showed that 4EM activates ER dependent gene transcription in a dose dependent manner in both ER subtypes. Further, 4EM- mediated transcription in ER-alpha, like estrogen, was enhance in the presense of co-activators, SRC-1 (steroid receptor coactivator-1), CBP (CREB binding proteins), and E6-AP (E6-associated protein) and inhibited by trans-4- hydroxytamoxifen (4HT). I found that 4EM was specific for ER and did not activate transcription of the progesterone receptor in HeLa cells.

This study assesses practices of college students (N=913) toward alternative medicine, relationships of students' health locus of control with practices of alternative medicine, and relationships of students' attitudes toward alternative medicine and health locus of control. A principal components factor analysis established construct validity of the author-designed Attitude Toward Alternative Medicine Scale, extracting three factors: Holistic Attitude/Control, Safety, and Satisfaction. Holistic Attitude/Control predicted use of alternative medicine (Wald =61.9, p < .01). A principal components factor analysis established construct validity of the Multidimensional Health Locus of Control Scale, extracting three factors: Internal Health Locus of Control, External-Chance, and External-Powerful Others. Internal Health Locus of Control significantly correlated with Holistic Attitude/Control (r = .35, p =.01).

The term quantization refers to the process of estimating a given probability by a discrete probability supported on a finite set. The quantization dimension Dr of a probability is related to the asymptotic rate at which the expected distance (raised to the rth power) to the support of the quantized version of the probability goes to zero as the size of the support is allowed to go to infinity. This assumes that the quantized versions are in some sense ``optimal'' in that the expected distances have been minimized. In this dissertation we give a short history of quantization as well as some basic facts. We develop a generalized framework for the quantization dimension which extends the current theory to include a wider range of probability measures. This framework uses the theory of thermodynamic formalism and the multifractal spectrum. It is shown that at least in certain cases the quantization dimension function D(r)=Dr is a transform of the temperature function b(q), which is already known to be the Legendre transform of the multifractal spectrum f(a). Hence, these ideas are all closely related and it would be expected that progress in one area could lead to new results in another. It would …

In 1824 a young Virginia aristocrat named Richard Thompson Archer migrated to Mississippi. Joining in the boom years of expansion in the Magnolia State in the 1830s, Archer built a vast cotton empire. He and his wife, Ann Barnes, raised a large family at Anchuca, their home plantation in Claiborne County, Mississippi. From there Richard Archer ruled a domain that included more than 500 slaves and 13,000 acres of land. On the eve of the Civil War he was one of the wealthiest men in the South. This work examines the life of Richard Archer from his origins in Amelia County, Virginia, to his death in Mississippi in 1867. It takes as its thesis the theme of Archer's life: his burdens as proprietor of a vast cotton empire and as father figure and provider for a large extended family. This theme weaves together the strands of Archer's life, including his rise to the position of great planter, his duties as husband and father, and his political beliefs and activities. Archer's story is told against the background of the history of Mississippi and of the South, from their antebellum heyday, through the Civil War, and into the early years of Reconstruction. …

For over one hundred years, the music-going public has reflected on the life and influence of America's “March King,” John Philip Sousa. His popularity as a bandleader was unprecedented, and his reputation as an entertainer captivated the imagination and intrigue of a nation. Sousa's fame was attained through the high standards showcased by his unparalleled concert organization, the Sousa Band. He is interminably linked to the march, and for his seventy-seven years he proved to be its prolific and outspoken champion. Sousa's songs, however, were among his favorite works, and their presence on concert programs reinforced a variety of programming that was the hallmark of his success. The Sousa Band served as a cultural and musical ambassador, and annual transcontinental tours brought music to people where they lived. Sousa's songs were highly anticipated concert features, and were presented by soprano soloists known as the “Ladies in White.” A chamber winds instrumentation, rather than employment of the full-forces of the Sousa Band, allowed for an appropriate musical balance between instruments and voice. The “Forgotten Songs of John Philip Sousa Project” involved the research, editing, and performance of songs housed in the Sousa Archives for Band Research at the University of Illinois. …

Accurate knowledge of stopping powers is essential for these for quantitative analysis and surface characterization of thin films using ion beam analysis (IBA). These values are also of interest in radiobiology and radiotherapy, and in ion- implantation technology where shrinking feature sizes puts high demands on the accuracy of range calculations. A theory that predicts stopping powers and ranges for all projectile-target combinations is needed. The most important database used to report the stopping powers is the SRIM/TRIM program developed by Ziegler and coworkers. However, other researchers report that at times, these values differ significantly from experimental values. In this study the stopping powers of Si, P and S ions have been measured in Ni, Cu, Ge and GaAs absorbers in the energy range ~ 2-10 MeV. For elemental films of Ni, Cu and Ge, the stopping of heavy ions was measured using a novel ERD (Elastic Recoil Detection) based technique. In which an elastically recoiled lighter atom is used to indirectly measure the energy of the incoming heavy ion using a surface barrier detector. In this way it was possible to reduce the damage and to improve the FWHM of the detector. The results were compared to SRIM-2000 predictions …

This study investigated what constitutes a moral development program in Buddhist temple preschools in Thailand. The researcher employed three qualitative methods: structured, in-depth interviews, observations of teachers' instruction, and document analysis of curriculum guides. Four Buddhist temple preschools were selected as the sites. Participants for interview included three abbots and one head nun, four principals, and twelve teachers. Participants for observations included four teachers of third year classes in each preschool. The study concentrated on four research questions: (a) what are the elements of the character education curriculum? (b) How do teachers teach moral development concepts and skills? (c) What are the teachers' perceptions of the moral development of third year preschoolers? (d) How do teachers assess their pupils' moral development? Key findings for the research questions were: character education was not a subject in the National Preschool Curriculum which was implemented in the Buddhist temple preschools. Core morality was integrated into every topic. The moral behaviors emphasized in the curriculum and the lesson plans included discipline, mindfulness, kindness, helpfulness, patience, honesty, respect, thriftiness, and politeness. The Buddhist concept of the process of moral development includes character education and meditation. The preschoolers were trained to pay respect to teachers and …

This thesis examines the issue of Texas annexation from the viewpoints of two southern cities: Richmond, Virginia, and New Orleans, Louisiana. It looks primarily at four major newspapers, two in each city: the Richmond Enquirer and the Richmond Whig; and the New Orleans Times-Picayune and the New Orleans Whig. These four newspapers were examined thoroughly from January 1844 to July 1845. In addition to the above newspapers, the Congressional Globe and national voting patterns on Texas annexation were examined. Analysis of the editorial articles in the above newspapers offers the best possibility of understanding public sentiment toward Texas annexation and the presidential election of 1844. The evidence examined in this study indicates that Texas annexation became a decisive issue in the presidential election of 1844. It also shows that, although the press and elements within both Democratic and Whig parties were aware that the slavery question was intricately linked to the Texas annexation issue, slavery and sectional politics were not the primary factors influencing annexation. Ultimately, fundamental concerns regarding western expansion in general, especially for the Whigs, and political party loyalty proved the decisive factors in the presidential election of 1844 and Texas annexation. The evidence gathered in this study …

Suppose L is a complete separable metric topological group (ring, field, etc.). L is topologically unique if the Polish topology on L is uniquely determined by its underlying algebraic structure. More specifically, L is topologically unique if an algebraic isomorphism of L with any other complete separable metric topological group (ring, field, etc.) induces a topological isomorphism. A local field is a locally compact topological field with non-discrete topology. The only local fields (up to isomorphism) are the real, complex, and p-adic numbers, finite extensions of the p-adic numbers, and fields of formal power series over finite fields. We establish the topological uniqueness of the special linear Lie algebras over local fields other than the complex numbers (for which this result is not true) in the context of complete separable metric Lie rings. Along the way the topological uniqueness of all local fields other than the field of complex numbers is established, which is derived as a corollary to more general principles which can be applied to a larger class of topological fields. Lastly, also in the context of complete separable metric Lie rings, the topological uniqueness of the special linear Lie algebra over the real division algebra of quaternions, …

In software testing, it is often desirable to find test inputs that exercise specific program features. Finding these inputs manually, is extremely time consuming, especially, when the software being tested is complex. Therefore, there have been numerous attempts automate this process. Random test data generation consists of generating test inputs at random, in the hope that they will exercise the desired software features. Often the desired inputs must satisfy complex constraints, and this makes a random approach seem unlikely to succeed. In contrast, combinatorial optimization techniques, such as those using genetic algorithms, are meant to solve difficult problems involving simultaneous satisfaction of many constraints.

Among the goals of the neuropsychological assessment are to detect the presence of brain damage, localize which areas of the brain may be dysfunctional and describe subsequent functional impairments. The sensitivity of neuropsychological instruments in carrying out these functions is a question of some debate. The purpose of this study is to determine the utility of lateralizing indicators from the WAIS-III, McCarron Assessment of Neuromuscular Development (MAND) and Haptic Visual Discrimination Test (HVDT), from the McCarron-Dial System Neuropsychological Assessment Battery (MDS), in ascertaining the presence or absence of brain damage as well as location of lesion. The classification accuracies of using performance level indicators from these tests and lateralizing indicators, alone and together, were compared.

A biological monitoring tool to assess water quality using bivalve gape behavior was developed and demonstrated. The purpose of this work was to develop methodologies for screening water quality appropriate to the goals of the watershed paradigm. A model of bivalve gape behavior based on prediction of behavior using autoregressive techniques was the foundation of the bivalve biomonitoring system. Current technology was used in developing the system to provide bivalve gape state data in a continuous real-time manner. A laboratory version of the system, including data collection and analysis hardware and software, was developed for use as a toxicological assay for determination of effective concentrations of toxicant(s) or other types of stress on bivalve gape behavior. Corbicula fluminea was monitored and challenged with copper, zinc, and chlorpyrifos using the system. Effective concentrations of 176±23µg/L copper, 768±412µg/L zinc, and 68µg/L chlorpyrifos were observed using a natural water with high dissolved organic carbon concentrations. A rugged field version of the bivalve biomonitoring system was developed and deployed in two locations. The field systems were fitted with a photovoltaic array, a single board computer, and a CDPD telemetry modem for robust remote operation. Data were telemetered at a time relevant rate of once …

Chapter I describes the purpose of the project, which was to develop a body of work that exhibits all that I am at this point in time. The questions I presented to myself were the following: 1. A successful piece is defined by what parameters? 2. What visual qualities indicate my passion for clay and my enthusiasm for life? Chapter II lists and explains the five parameters of a successful piece, which are composition, firing, mark making, color contrast, and movement. Furthermore there is an explanation on how these parameters visually display my passion for clay and my enthusiasm for life. Chapter III is a summary concluding that by discovering my five parameters of a successful piece I now understand the elements that I am searching for in my work. My work will grow from this understanding as long as I have the same passion for clay and enthusiasm for life.

Neuringer, Deiss, and Olson (2000) was replicated and extended to determine the effect of variability contingencies on task acquisition for twelve 7-9 year old children. Subjects first learned to press a computer's shift keys with increasing response variation. Each subject was then exposed to one of three experimental conditions during which they received a point for target responses. Variability condition subjects received additional points on a variable interval schedule for nontarget responses occurring less than 3% of the time. The any condition subjects received additional points on a variable interval schedule for any nontarget response. Control subjects received points only for target responses. All variability condition and two control subjects learned the target response. All any condition subjects and two control subjects did not.

It has been noticed in the past five years that the Internet use has been troubled by the lack of sufficient security and a legal framework to enable electronic commerce to flourish. Despite these shortcomings, governments, businesses and individuals are using the Internet more often as an inexpensive and ubiquitous means to disseminate and obtain information, goods and services. The Internet is insecure -- potentially millions of people have access, and "hackers" can intercept anything traveling over the wire. There is no way to make it a secure environment; it is, after all, a public network, hence the availability and affordability. In order for it to serve our purposes as a vehicle for legally binding transactions, efforts must be directed at securing the message itself, as opposed to the transport mechanism. Digital signatures have been evolved in the recent years as the best tool for ensuring the authenticity and integrity of critical information in the so called "paperless office". A model using XML digital signatures is developed and the level of security provided by this model in the real world scenario is outlined.

The purpose of this study determined whether use of student random drug testing provided an effective means to reduce drug usage by secondary school students. The participants included 50,214 7th through 12th grade students in 12 selected public schools. All school districts participated in the Texas School Survey of Substance Use in 1994, 1996, 1998, and 2000. The six districts in the experimental group used drug testing as a method of reducing drug usage among students. The six districts in the control group did not use drug testing. Although athletes and students involved in extracurricular activities remain the focus of random dug testing, this research focused on an entire school population to determine whether drug testing only a select group of students reduced reported drug usage in the entire school. Two questions guided the research: First, does the use of random drug testing have an impact on student drug usage? Second, does the year of implementation of random drug testing have an impact on students' self-reported drug usage? The findings for each research question were categorized according to nine illegal drugs. The researcher used a one-way repeated measures factorial design. The data were analyzed via the univariate (split-plot) 2 x …

This problem in lieu thesis is considering a TCP client H1 making a connection to distant server S and is downloading a file. In the midst of the downloading, if H1 crashes, the TCP connection from H1 to S is lost. In the future, if H1 restarts, the TCP connection from H1 to S will be reestablished and the file will be downloaded again. This cannot happen until host H1 restarts. Now consider a situation where there is a standby host H2 for the host H1. H1 and H2 monitor the health of each other by heartbeat messages (like SCTP). If H2 detects the failure of H1, then H2 takes over. This implies that all resources assigned to H1 are now reassigned or taken over by H2. The host H1 and H2 transmit data between each other when any one of it crashed. Throughout the data transmission process, heart beat chunk is exchanged between the hosts when one of the host crashes. In particular, the IP addresses that were originally assigned to H1 are assigned to H2. In this scenario, movement of the TCP connection between H1 and S to a connection between H2 and S without disrupting the TCP …

Many tools have been developed to assess global, national or regional sustainable development policies. However, as governments develop sustainable policies, individuals must also feel empowered to affect their personal impact on the planet. This thesis integrates three sustainability concepts that lend themselves to individual sustainability: The natural step, life cycle assessment, and the ecological footprint. TNS serves to provide the meaning and substance toward sustainable development. LCA helps provide the framework for assessing sustainability. The EF calculator determines the driving components and measures the qualitative decisions made through TNS and LCA. From the analysis of the household footprint calculator a simplified footprint calculator was developed to assist individuals and communities in setting benchmarks and goals as they move away from over-consumption and towards a sustainable lifestyle.