Past literature suggests that working mothers are at an increased risk for experiencing role strain compared to other employed adults. The current study investigated attitudes and beliefs of 783 working mothers of 15-month-old children using data from the National Institute of Child Health and Human Development's Study of Early Child Care and Youth Development. Working mothers' levels of role strain was associated with perceived social support, attitudes toward maternal employment, job and parental role quality, financial stress, and depression. Negative attitudes toward maternal employment predicted maternal separation anxiety, while positive attitudes toward employment did not affect separation anxiety. These findings have implications for the importance of decreasing role strain in working mothers.