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An Analysis of the Habitat of the Greater One-Horned Rhinoceros Rhinoceros Unicornis (Mammalia: Perissodactyla: Rhinocerotidae) at the Chitwan National Park, Nepal (open access)

An Analysis of the Habitat of the Greater One-Horned Rhinoceros Rhinoceros Unicornis (Mammalia: Perissodactyla: Rhinocerotidae) at the Chitwan National Park, Nepal

This article uses geographic information systems and landscape-level data obtained from remote sensing sources to build a habitat suitability index model for the Greater One-horned Rhinoceros.
Date: September 26, 2014
Creator: Thapa, Vivek; Acevedo, Miguel F. & Limbu, Kul P.
Object Type: Article
System: The UNT Digital Library
Effects of Sertraline Exposure on Fathead Minnow (Pimephales promelas) Steroidogenesis (open access)

Effects of Sertraline Exposure on Fathead Minnow (Pimephales promelas) Steroidogenesis

Sertraline is a selective serotonin reuptake inhibitor (SSRI) that is widely used for the treatment of depression and anxiety. Due to the abundant therapeutic use of sertraline, low levels have been detected in municipal wastewater effluents suggesting that aquatic organisms may be exposed. The purpose of this study was to evaluate the steroidogenic effects of sertraline on larval (FHM) and adult female fathead minnows (FFHM), Pimephales promelas. Larval FHM were exposed to 0.1, 1, and 10 µg/L sertraline for 28 days and analyzed via RT-qPCR for differential expression of 11β-Hydroxysteroid dehydrogenase (11β-HSD), 20β-Hydroxysteroid dehydrogenase (20β-HSD), aromatase (CYP19), and nuclear thyroid receptor alpha (TRα). FFHM were exposed to 3 or 10 µg/L sertraline for 7 days with the brain and ovary excised at exposure termination. Juvenile FHM exposed to 0.1 μg/L sertraline had a significant upregulation of both 20β-HSD and TRα. FFHM exposed to 10 µg/L sertraline had a significant upregulation of 11β-HSD expression in brain tissue, while no steroidogenic changes were observed in the FFHM ovary. Similarly, in FFHM brain tissue, CYP19 and 20β-HSD expression levels were significantly higher in fish exposed to 10µg/L sertraline compared to control. The significance of these findings with respect to survival, growth and reproduction …
Date: December 2014
Creator: Carty, Dennis R.
Object Type: Thesis or Dissertation
System: The UNT Digital Library