Analysis of Metabolic Pathways and Fluxes in a Newly Discovered Thermophilic and Ethanol-Tolerant Geobacillus Strain (open access)

Analysis of Metabolic Pathways and Fluxes in a Newly Discovered Thermophilic and Ethanol-Tolerant Geobacillus Strain

A recently discovered thermophilic bacterium, Geobacillus thermoglucosidasius M10EXG, ferments a range of C5 (e.g., xylose) and C6 sugars (e.g., glucose) and istolerant to high ethanol concentrations (10percent, v/v). We have investigated the central metabolism of this bacterium using both in vitro enzyme assays and 13C-based flux analysis to provide insights into the physiological properties of this extremophile and explore its metabolism for bio-ethanol or other bioprocess applications. Our findings show that glucose metabolism in G. thermoglucosidasius M10EXG proceeds via glycolysis, the pentose phosphate pathway, and the TCA cycle; the Entner?Doudoroff pathway and transhydrogenase activity were not detected. Anaplerotic reactions (including the glyoxylate shunt, pyruvate carboxylase, and phosphoenolpyruvate carboxykinase) were active, but fluxes through those pathways could not be accuratelydetermined using amino acid labeling. When growth conditions were switched from aerobic to micro-aerobic conditions, fluxes (based on a normalized glucose uptake rate of 100 units (g DCW)-1 h-1) through the TCA cycle and oxidative pentose phosphate pathway were reduced from 64+-3 to 25+-2 and from 30+-2 to 19+-2, respectively. The carbon flux under micro-aerobic growth was directed formate. Under fully anerobic conditions, G. thermoglucosidasius M10EXG used a mixed acid fermentation process and exhibited a maximum ethanol yield of 0.38+-0.07 mol mol-1 …
Date: January 20, 2009
Creator: Tang, Yinjie J.; Sapra, Rajat; Joyner, Dominique; Hazen, Terry C.; Myers, Samuel; Reichmuth, David et al.
Object Type: Article
System: The UNT Digital Library

Concentrating Photovoltaics

Solar is growing rapidly, and the concentrating photovoltaics industry-both high- and low-concentration cell approaches-may be ready to ramp production in 2009.
Date: January 20, 2009
Creator: Kurtz, S.
Object Type: Presentation
System: The UNT Digital Library
Final report SBIR Phase II. High current density,(Jc), low AC loss, low cost, Internal-Tin Superconductor (open access)

Final report SBIR Phase II. High current density,(Jc), low AC loss, low cost, Internal-Tin Superconductor

Final report of SBIR to develop an economical process that can produce the best material for high field magnets to be used in the next generation of accelerators
Date: January 20, 2009
Creator: Gregory, Eric
Object Type: Report
System: The UNT Digital Library
Hanford Site Environmental Surveillance Master Sampling Schedule for Calendar Year 2009 (open access)

Hanford Site Environmental Surveillance Master Sampling Schedule for Calendar Year 2009

Environmental surveillance of the Hanford Site and surrounding areas is conducted by the Pacific Northwest National Laboratory for the U.S. Department of Energy. Sampling is conducted to evaluate levels of radioactive and nonradioactive pollutants in the Hanford environs, as required in DOE Order 450.1 and DOE Order 5400.5. This document contains the calendar year 2009 schedule for the routine collection of samples for the Surface Environmental Surveillance Project and Drinking Water Monitoring Project. Each section includes sampling locations, sampling frequencies, sample types, and analyses to be performed. In some cases, samples are scheduled on a rotating basis. If a sample will not be collected in 2009, the anticipated year for collection is provided. Maps showing approximate sampling locations are included for media scheduled for collection in 2009.
Date: January 20, 2009
Creator: Bisping, Lynn E.
Object Type: Report
System: The UNT Digital Library
Improving Consistency of Performance Assessments in the Doe Complex (open access)

Improving Consistency of Performance Assessments in the Doe Complex

The low-level waste (LLW) performance assessment (PA) process has been traditionally focused on disposal facilities at a few United States Department of Energy (USDOE) sites and commercial disposal facilities. In recent years, there has been a dramatic increase in the scope of the use of PA-like modeling approaches, involving multiple activities, facilities, contractors and regulators. The scope now includes, for example: (1) National Environmental Policy Act (NEPA) assessments, (2) CERCLA disposal cells, (3) Waste Determinations and High-Level Waste (HLW) Closure activities, (4) Potential on-site disposal of Transuranic (TRU) waste, and (5) In-situ decommissioning (including potential use of existing facilities for disposal). The dramatic increase in the variety of activities requiring more detailed modeling has resulted in a similar increase in the potential for inconsistency in approaches both at a site and complexwide scale. This paper includes a summary of USDOE Environmental Management (EM) sponsored initiatives and activities for improved consistency. New initiatives entitled the Performance Assessment Community of Practice and Performance Assessment Assistance Team are also introduced.
Date: January 20, 2009
Creator: Seitz, R. & Elmer Wilhite, E.
Object Type: Article
System: The UNT Digital Library
MEASUREMENT OF WIND SPEED FROM COOLING LAKE THERMAL IMAGERY (open access)

MEASUREMENT OF WIND SPEED FROM COOLING LAKE THERMAL IMAGERY

The Savannah River National Laboratory (SRNL) collected thermal imagery and ground truth data at two commercial power plant cooling lakes to investigate the applicability of laboratory empirical correlations between surface heat flux and wind speed, and statistics derived from thermal imagery. SRNL demonstrated in a previous paper [1] that a linear relationship exists between the standard deviation of image temperature and surface heat flux. In this paper, SRNL will show that the skewness of the temperature distribution derived from cooling lake thermal images correlates with instantaneous wind speed measured at the same location. SRNL collected thermal imagery, surface meteorology and water temperatures from helicopters and boats at the Comanche Peak and H. B. Robinson nuclear power plant cooling lakes. SRNL found that decreasing skewness correlated with increasing wind speed, as was the case for the laboratory experiments. Simple linear and orthogonal regression models both explained about 50% of the variance in the skewness - wind speed plots. A nonlinear (logistic) regression model produced a better fit to the data, apparently because the thermal convection and resulting skewness are related to wind speed in a highly nonlinear way in nearly calm and in windy conditions.
Date: January 20, 2009
Creator: Garrett, A; Robert Kurzeja, R; Eliel Villa-Aleman, E; Cary Tuckfield, C & Malcolm Pendergast, M
Object Type: Article
System: The UNT Digital Library
Prototype Testing for a Copper Rotatable Collimator for the LHC Collimation Upgrade (open access)

Prototype Testing for a Copper Rotatable Collimator for the LHC Collimation Upgrade

The Phase II upgrade to the LHC collimation system calls for complementing the robust Phase I graphite collimators with high Z Phase II collimators. The design for the collimation upgrade has not been finalized. One option is to use metallic rotatable collimators and testing of this design will be discussed here. The Phase II collimators must be robust in various operating conditions and accident scenarios. A prototype collimator jaw referred to as RC0 has been tested for both mechanical and thermal compliance with the design goals. Thermal expansion bench-top tests are compared to ANSYS simulation results. The prototype has also been tested in vacuum bake-out to confirm compliance with the LHC vacuum spec. CMM equipment has been used to verify the flatness of the jaw surface after heat tests and bake-out.
Date: January 20, 2009
Creator: Smith, Jeffrey Claiborne; Anzalone, Gene; Doyle, Eric; Keller, Lewis; Lundgren, Steven; Markiewicz, Thomas Walter et al.
Object Type: Report
System: The UNT Digital Library
Stochastic Boundary, Diffusion, Emittance Growth and Lifetime calculation for the RHIC e-lens (open access)

Stochastic Boundary, Diffusion, Emittance Growth and Lifetime calculation for the RHIC e-lens

To compensate the large tune shift and tune spread generated by the head-on beam-beam interactions in polarized proton operation in the Relativistic Heavy Ion Collider (RHIC), a low energy electron beam with proper Gaussian transverse profiles was proposed to collide head-on with the proton beam. In this article, using a modified version of SixTrack [1], we investigate stability of the single particle in the presence of head-on beam-beam compensation. The Lyapunov exponent and action diffusion are calculated and compared between the cases without and with beam-beam compensation for two different working points and various bunch intensities. Using the action diffusion results the emittance growth rate and lifetime of the proton beam is also estimated for the different scenarios.
Date: January 20, 2009
Creator: Abreu,N.P.; Fischer, W.; Luo, Y. & Robert-Demolaize, G.
Object Type: Report
System: The UNT Digital Library
Two-photon double ionization of H2 at 30 eV using Exterior Complex Scaling (open access)

Two-photon double ionization of H2 at 30 eV using Exterior Complex Scaling

Calculations of fully differential cross sections for two-photon double ionization of the hydrogen molecule with photons of 30 eV are reported. The results have been obtained by using the method of exterior complex scaling, which allows one to construct essentially exact wave functions that describe thedouble continuum on a large, but finite, volume. The calculated cross sections are compared with those previously obtained by Colgan et al., and discrepancies are found for specific molecular orientations and electron ejection directions.
Date: January 20, 2009
Creator: Morales, Felipe; Martin, Fernando; Horner, Daniel; Rescigno, Thomas N. & McCurdy, C. William
Object Type: Article
System: The UNT Digital Library
81st Texas Legislature, House Concurrent Resolution, House Bill 24 (open access)

81st Texas Legislature, House Concurrent Resolution, House Bill 24

Concurrent resolution introduced by the Texas House of Representatives and Senate relating to recognizing February 17, 2009, as Midland-Odessa Day at the State Capitol
Date: February 20, 2009
Creator: Texas. Legislature. House of Representatives.
Object Type: Legislative Document
System: The Portal to Texas History
81st Texas Legislature, House Concurrent Resolution, House Bill 25 (open access)

81st Texas Legislature, House Concurrent Resolution, House Bill 25

Concurrent resolution introduced by the Texas House of Representatives and Senate relating to honoring Paris Police Chief Karl Louis for his service and congratulating him on his retirement.
Date: February 20, 2009
Creator: Texas. Legislature. House of Representatives.
Object Type: Legislative Document
System: The Portal to Texas History
81st Texas Legislature, House Concurrent Resolution, House Bill 31 (open access)

81st Texas Legislature, House Concurrent Resolution, House Bill 31

Concurrent resolution introduced by the Texas House of Representatives and Senate relating to honoring Barbara Smith Conrad on her career as a world-renowned opera singer.
Date: February 20, 2009
Creator: Texas. Legislature. House of Representatives.
Object Type: Legislative Document
System: The Portal to Texas History
81st Texas Legislature, House Concurrent Resolution, House Bill 37 (open access)

81st Texas Legislature, House Concurrent Resolution, House Bill 37

Concurrent resolution introduced by the Texas House of Representatives and Senate relating to honoring Olympic gymnastics champion Nastia Liukin of Parker.
Date: February 20, 2009
Creator: Texas. Legislature. House of Representatives.
Object Type: Legislative Document
System: The Portal to Texas History
81st Texas Legislature, House Concurrent Resolution, House Bill 38 (open access)

81st Texas Legislature, House Concurrent Resolution, House Bill 38

Concurrent resolution introduced by the Texas House of Representatives and Senate relating to welcoming Leadership Midlothian to the State Capitol.
Date: February 20, 2009
Creator: Texas. Legislature. House of Representatives.
Object Type: Legislative Document
System: The Portal to Texas History
Caustic Recycle from Hanford Tank Waste Using NaSICON Ceramic Membrane Salt Splitting Process (open access)

Caustic Recycle from Hanford Tank Waste Using NaSICON Ceramic Membrane Salt Splitting Process

A family of inorganic ceramic materials, called sodium (Na) Super Ion Conductors (NaSICON), has been studied at Pacific Northwest National Laboratory (PNNL) to investigate their ability to separate sodium from radioactively contaminated sodium salt solutions for treating U.S. Department of Energy (DOE) tank wastes. Ceramatec Inc. developed and fabricated a membrane containing a proprietary NAS-GY material formulation that was electrochemically tested in a bench-scale apparatus with both a simulant and a radioactive tank-waste solution to determine the membrane performance when removing sodium from DOE tank wastes. Implementing this sodium separation process can result in significant cost savings by reducing the disposal volume of low-activity wastes and by producing a NaOH feedstock product for recycle into waste treatment processes such as sludge leaching, regenerating ion exchange resins, inhibiting corrosion in carbon-steel tanks, or retrieving tank wastes.
Date: February 20, 2009
Creator: Fountain, Matthew S.; Kurath, Dean E.; Sevigny, Gary J.; Poloski, Adam P.; Pendleton, J.; Balagopal, S. et al.
Object Type: Report
System: The UNT Digital Library
Defense Management: Actions Needed to Address Stakeholder Concerns, Improve Interagency Collaboration, and Determine Full Costs Associated with the U.S. Africa Command (open access)

Defense Management: Actions Needed to Address Stakeholder Concerns, Improve Interagency Collaboration, and Determine Full Costs Associated with the U.S. Africa Command

A letter report issued by the Government Accountability Office with an abstract that begins "In February 2007, the President directed the Department of Defense (DOD) to establish the U.S. Africa Command (AFRICOM) to help strengthen U.S. security cooperation with African nations and bring peace and stability to the continent. For this review, GAO assessed DOD's (1) efforts to establish the command and communicate its mission, (2) progress in integrating personnel from other U.S. government agencies into AFRICOM, and (3) plans and costs for establishing a permanent headquarters and supporting offices in Africa. In assessing DOD's efforts to establish AFRICOM, GAO analyzed relevant documentation and obtained perspectives from the combatant commands, military services, Office of the Secretary of Defense, Department of State (State), U. S. Agency for International Development, and nongovernmental organizations."
Date: February 20, 2009
Creator: United States. Government Accountability Office.
Object Type: Report
System: The UNT Digital Library
Defense Management: DOD Needs to Increase Attention on Fuel Demand Management at Forward-Deployed Locations (open access)

Defense Management: DOD Needs to Increase Attention on Fuel Demand Management at Forward-Deployed Locations

A letter report issued by the Government Accountability Office with an abstract that begins "The Department of Defense (DOD) relies heavily on petroleum-based fuel to sustain its forward-deployed locations--particularly those that are not connected to local power grids. While weapon platforms require large amounts of fuel, DOD reports that the single largest battlefield fuel consumer is generators, which provide power for base support activities such as cooling, heating, and lighting. Transporting fuel to forward-deployed locations presents an enormous logistics burden and risk, including exposing fuel truck convoys to attack. GAO was asked to address DOD's (1) efforts to reduce fuel demand at forward-deployed locations and (2) approach to managing fuel demand at these locations. This review focused on locations within Central Command's area of responsibility. GAO visited DOD locations in Kuwait and Djibouti to learn about fuel reduction efforts and challenges facing these locations."
Date: February 20, 2009
Creator: United States. Government Accountability Office.
Object Type: Report
System: The UNT Digital Library
Documentation Package for the RFID Temperature Monitoring System (Of Model 9977 Packages at NTS). (open access)

Documentation Package for the RFID Temperature Monitoring System (Of Model 9977 Packages at NTS).

The technical basis for extending the Model 9977 shipping package periodic maintenance beyond the one-year interval to a maximum of five years is based on the performance of the O-ring seals and the environmental conditions. The DOE Packaging Certification Program (PCP) has tasked Argonne National Laboratory to develop a Radio-Frequency Identification (RFID) temperature monitoring system for use by the facility personnel at DAF/NTS. The RFID temperature monitoring system, depicted in the figure below, consists of the Mk-1 RFId tags, a reader, and a control computer mounted on a mobile platform that can operate as a stand-alone system, or it can be connected to the local IT network. As part of the Conditions of Approval of the CoC, the user must complete the prescribed training to become qualified and be certified for operation of the RFID temperature monitoring system. The training course will be administered by Argonne National Laboratory on behalf of the Headquarters Certifying Official. This is a complete documentation package for the RFID temperature monitoring system of the Model 9977 packagings at NTS. The documentation package will be used for training and certification. The table of contents are: Acceptance Testing Procedure of MK-1 RFID Tags for DOE/EM Nuclear Materials …
Date: February 20, 2009
Creator: Chen, K.; Tsai, H. & Sciences, Decision and Information
Object Type: Report
System: The UNT Digital Library
DOE Award No. DE-FC36-03GO13108 Novel Non-Precious Metal Catalysts for PEMFC: Catalyst Selection Through Molecular Modeling and Durability Studies Final Report (September 2003 – October 2008) (open access)

DOE Award No. DE-FC36-03GO13108 Novel Non-Precious Metal Catalysts for PEMFC: Catalyst Selection Through Molecular Modeling and Durability Studies Final Report (September 2003 – October 2008)

The objective of this project is to develop novel non-precious metal electrocatalysts for oxygen reduction reaction (ORR), and demonstrate the potential of the catalysts to perform at least as good as conventional Pt catalysts currently in use in polymer electrolyte membrane fuel cell (PEMFC) with a cost at least 50 % less than a target of 0.2 g (Pt loading)/peak kW and with durability > 2,000 h operation with less than 10 % power degradation. A novel nitrogen-modified carbon-based catalyst was obtained by modifying carbon black with nitrogen-containing organic precursor in the absence of transition metal precursor. The catalyst shows the onset potential of approximately 0.76 V (NHE) for ORR and the amount of H2O2 of approximately 3% at 0.5 V (NHE). Furthermore, a carbon composite catalyst was achieved through the high-temperature pyrolysis of the precursors of transition metal (Co and Fe) and nitrogen supported on the nitrogen-modified carbon-based catalyst, followed by chemical post-treatment. This catalyst showed an onset potential for ORR as high as 0.87 V (NHE), and generated less than 1 % of H2O2. The PEM fuel cell exhibited a current density of 2.3 A cm-2 at 0.2 V for a catalyst loading of 6.0 mg cm-2. No …
Date: February 20, 2009
Creator: Popov, Branko N.
Object Type: Report
System: The UNT Digital Library
Duck Valley Reservoirs Fish Stocking and O&M, Annual Progress Report 2007-2008. (open access)

Duck Valley Reservoirs Fish Stocking and O&M, Annual Progress Report 2007-2008.

The Duck Valley Reservoirs Fish Stocking and Operations and Maintenance Project (DV Fisheries) is an ongoing resident fish program that serves to partially mitigate the loss of anadromous fish that resulted from downstream construction of the federal hydropower system. The project's goals are to enhance subsistence fishing and educational opportunities for Tribal members of the Shoshone-Paiute Tribes and provide fishing opportunities for non-Tribal members. In addition to stocking rainbow trout (Oncorhynchus mykiss) in Mountain View (MVR), Lake Billy Shaw (LBS), and Sheep Creek Reservoirs (SCR), the program is also designed to: maintain healthy aquatic conditions for fish growth and survival, provide superior facilities with wilderness qualities to attract non-Tribal angler use, and offer clear, consistent communication with the Tribal community about this project as well as outreach and education within the region and the local community. Tasks for this performance period fall into three categories: operations and maintenance, monitoring and evaluation, and public outreach. Operation and maintenance of the three reservoirs include maintaining fences, roads, dams and all reservoir structures, feeder canals, water troughs, stock ponds, educational signs, vehicles, equipment, and restroom facilities. Monitoring and evaluation activities include creel, gillnet, wildlife, and bird surveys, water quality and reservoir structures monitoring, …
Date: February 20, 2009
Creator: Sellman, Jake & Perugini, Carol
Object Type: Report
System: The UNT Digital Library
Estimates of Carbon Mitigation Potential from Agricultural and Forestry Activities (open access)

Estimates of Carbon Mitigation Potential from Agricultural and Forestry Activities

This report presents a brief overview of the available estimates from U.S. Department of Agriculture (USDA) and U.S. Environmental Protection Agency (EPA) carbon mitigation studies, and then discusses some of the limitations of the available data and modeling results. It includes informationa bout estimated current emissions and sequestration, estimated addtional potential sequestration, limitations of mitigation potential estimates, and considerations for Congress.
Date: February 20, 2009
Creator: Johnson, Renée; Gorte, Ross W.; Yacobucci, Brent D. & Schnepf, Randy
Object Type: Report
System: The UNT Digital Library
Estimates of Carbon Mitigation Potential from Agricultural and Forestry Activities (open access)

Estimates of Carbon Mitigation Potential from Agricultural and Forestry Activities

The report is organized into three parts. The first provides a brief overview of the role of the agriculture and forestry sectors within the broader climate change debate, describing available estimates of current GHG emissions and carbon sequestration in the farm and forestry sectors. The second describes available data and information on the potential for carbon storage (tonnage) by type of farming and forestry activity, and presents available estimates of the carbon sequestration potential in these sectors. The final part discusses some of the limitations of available estimates of GHG mitigation potential in the agriculture and forestry sectors, focusing on recent policy and market changes and other types of modeling uncertainties that could limit the accuracy of available mitigation projections.
Date: February 20, 2009
Creator: Johnson, Renée; Gorte, Ross W.; Yacobucci, Brent D. & Schnepf, Randy
Object Type: Report
System: The UNT Digital Library
Estimating Filtering Errors Using the Peano Kernel Theorem (open access)

Estimating Filtering Errors Using the Peano Kernel Theorem

The Peano Kernel Theorem is introduced and a frequency domain derivation is given. It is demonstrated that the application of this theorem yields simple and accurate formulas for estimating the error introduced into a signal by filtering it to reduce noise.
Date: February 20, 2009
Creator: Blair, Jerome
Object Type: Article
System: The UNT Digital Library
Farm and Food Support Under USDA's Section 32 Program (open access)

Farm and Food Support Under USDA's Section 32 Program

The 110th Congress in June 2008 passed a new omnibus farm bill (P.L. 110-246). Provisions in this new law now spell out more explicitly how the Secretary is to use the annual Section 32 appropriation. Section 32 of the act of August 24, 1935 (P.L. 74-320 as amended; 7 U.S.C. 612c) authorizes a permanent appropriation equal to 30% of annual U.S. customs receipts.
Date: February 20, 2009
Creator: Becker, Geoffrey S.
Object Type: Report
System: The UNT Digital Library