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FY02 CBNP Annual Report: Discovery of DNA Signature of Biothreat Detection Using Suppression Subtractive Hybridization
Our goal is to develop robust DNA signatures for rapid and specific DNA-based detection platforms that can be employed by CBNP to detect a wide range of potential agents. Our approach has resulted in highly specific DNA signatures for Yersina pestis, Bacillus anthracis and Brucella species. Furthermore, this approach can be applied to any genome (even uncharacterized ones), which facilitates DNA signature development for detection of newly emerging pathogens. We are using suppression subtractive hybridization (SSH) as a tool to define large DNA regions specific to multiple biothreat pathogens by comparing them to genomes of the most closely related organisms. This approach has become increasingly accurate as we continue to find new, distinctive strains and ever-closer near-neighbors. With the huge costs incurred by whole genome sequencing, it is not possible to sequence each new bacterial genome. However, it is completely practical to identify genome differences in the laboratory using SSH, and becomes especially useful when comparing new strains to previously sequenced genomes.
Date:
November 19, 2002
Creator:
Andersen, G L & Radnedge, L
Object Type:
Report
System:
The UNT Digital Library
Results of Performance Evaluation Testing of Electrical Leak-Detection Methods at the Hanford Mock Tank Site--FY 2002-2003
Application of two electrical resistivity methods at the Hanford Site Mock Tank during 2002, indicate the viability of the methods as possible leak-detection tools for SST retrieval operations. Electrical Resistivity Tomography and High-Resolution Resistivity were used over a 109-day period to detect leakage of a waste simulant beneath the tank. The results of the test indicate that both of these two methods, and subset methods may be applicable to SST leak detection.
Date:
February 1, 2003
Creator:
Barnett, D. Brent; Gee, Glendon W.; Sweeney, Mark D.; Johnson, Michael D.; Medina, Victor F.; Mendoza, Donaldo P. et al.
Object Type:
Report
System:
The UNT Digital Library
Appropriations for FY2002: Energy and Water Development
Appropriations are one part of a complex federal budget process that includes budget resolutions, appropriations (regular, supplemental, and continuing) bills, rescissions, and budget reconciliation bills. This report is a guide to one of the 13 regular appropriations bills that Congress passes each year. It is designed to supplement the information provided by the House and Senate Appropriations Subcommittees on Energy and Water.
Date:
February 5, 2002
Creator:
Behrens, Carl E. & Humphries, Marc
Object Type:
Report
System:
The UNT Digital Library
United Nations Commission on Human Rights (UNCHR): U.S. Membership
This short report provides an overview of the U.N. Commission on Human Rights (UNCHR and Administration and Congressional responses to recent developments.
Date:
June 4, 2001
Creator:
Bite, Vita
Object Type:
Report
System:
The UNT Digital Library
FY02 Final Report on Phytoremediation of Chlorinated Ethenes in Southern Sector Sediments of the Savannah River Site
This final report details the operations and results of a 3-year Seepline Phytoremediation Project performed adjacent to Tims Branch, which is located in the Southern Sector of the Savannah River Site (SRS) A/M Area. Phytoremediation is a process where interactions between vegetation, associated microorganisms, and the host substrate combine to effectively degrade contaminated soils, sediments, and groundwater. Phytoremediation is a rapidly developing technology that shows promise for the effective and safe cleanup of certain hazardous wastes. It has the potential to remediate numerous volatile organic compounds (VOCs). Extensive characterization work has demonstrated that two VOCs, tetrachloroethylene (PCE) and trichloroethylene (TCE) are the major components of the VOC-contaminated groundwater that is migrating through the Southern Sector and Tims Branch seepline area (WSRC, 1999). The PCE and TCE are chlorinated ethenes (CE), and have been detected in seepline soils and ground water adjacent to the ecologically-sensitive Tims Branch seepline area.
Date:
January 30, 2004
Creator:
Brigmon, R..L.
Object Type:
Report
System:
The UNT Digital Library
LDRD 26573 Ultra-Low Power Spread Spectrum Receiver, FY02 Final Report
This report describes the development of an ultra-low power spread spectrum receiver based on a programmable surface acoustic wave (SAW) correlator. This work was funded under LDRD 02-26573, Ultra-Low Power Spread Spectrum Receiver. The approach taken in this project uses direct demodulation of a radio frequency (RF) signal from carrier frequency to data frequency. This approach was taken to reduce power consumption and size. The design is based on the technique of correlating the received RF signal with the preprogrammed spreading code. The system requirements, applications, design methodology, and testing results are all documented in the following pages.
Date:
October 2002
Creator:
Brocato, Robert W.
Object Type:
Report
System:
The UNT Digital Library
Cash and Noncash Benefits for Persons with Limited Income: Eligibility Rules, Recipient and Expenditure Data, FY2000-FY2002
This report provides basic eligibility rules, recipient numbers, and FY2000-FY2002 expenditure data for 80 programs that have provided cash or non-cash benefits to low-income persons.
Date:
November 25, 2003
Creator:
Burke, Vee
Object Type:
Report
System:
The UNT Digital Library
Review of the Sandia National Laboratories -- Albuquerque, New Mexico DOE/DP Critical Skills Development Programs FY '02
Sandia National Laboratories has developed a portfolio of programs to address the critical skills needs of the DP labs, as identified by the 1999 Chiles Commission Report. The goals are to attract and retain the best and the brightest students and transition them into Sandia--and DP Complex--employees. The US Department of Energy/Defense Programs University Partnerships funded seven laboratory critical skills development programs in FY02. This report provides a qualitative and quantitative evaluation of these programs and their status.
Date:
February 1, 2003
Creator:
CLARK, KATHERINE SUE
Object Type:
Report
System:
The UNT Digital Library
Summary of Hanford Subsurface Air Flow and Extraction (SAFE) Activities for Fiscal Year 2002
Potential leak detection, monitoring, and mitigation techniques are being developed to support Hanford single-shell tank waste retrieval operations. In July and August 2001, Pacific Northwest National Laboratory demonstrated several of these technologies for CH2M HILL Hanford Group, Inc., at the Mock Tank Site in the 200 East Area. These subsurface air flow and extraction (SAFE) technologies use air injection and extraction wells to create an advective air flowfield beneath a tank. SAFE includes the following technologies: 1) leak detection--in-tank tracers, flowfield disturbance, radon displacement, and tank waste vapors; 2) leak monitoring--partitioning tracer method and reactive tracers; 3) leak mitigation--soil desiccation before and after leakage and in situ gaseous reduction; and 4) subsurface characterization--interfacial tracers. This report provides an overview of these technologies and discusses the FY 2001 demonstration activities at the Mock Tank Site, their results, and implications for future work.
Date:
March 20, 2002
Creator:
Cameron, Richard J.; Evans, John C.; Johnson, Michael D. & Liikala, Terry L.
Object Type:
Report
System:
The UNT Digital Library
Defense Authorization and Appropriations Bills: A Chronology, FY1970-FY2005
This report is a research aid, which lists the Department of Defense (DOD) authorization bills (Table 1) and appropriations bills (Table 2).
Date:
December 14, 2004
Creator:
Carter, Linwood B. & Coipuram, Thomas, Jr.
Object Type:
Report
System:
The UNT Digital Library
Defense Authorization and Apropriations Bills: A Chronology, FY1970-FY2006
This report is a research aid, which lists the DOD authorization bills (Table 1) and appropriations bills (Table 2). This report includes all the pertinent information on the passage of these bills through the legislative process: bill numbers, report numbers, dates reported and passed, recorded vote numbers and vote tallies, dates of passage of the conference reports with their numbers and votes, vetoes, substitutions, dates of final passage, and public law numbers. Table 3 shows real growth or decline in national defense funding for FY1940-FY2009. Table 4 gives a more detailed picture of both regular and supplemental defense appropriations from the 103rd Congress to the present (FY1993-FY2005). Table 5 shows the President’s DOD appropriations budget requests for FY1950-FY2005 vs. final amount enacted.
Date:
June 23, 2005
Creator:
Carter, Linwood B. & Coipuram, Thomas, Jr.
Object Type:
Report
System:
The UNT Digital Library
Sandia National Laboratories Laboratory Directed Research and Development Program Annual Report: 2002
None
Date:
March 1, 2003
Creator:
Chavez, Donna L.
Object Type:
Report
System:
The UNT Digital Library
Appropriations for FY2002: U.S. Department of Agriculture and Related Agencies
This report is a guide to one of the 13 regular appropriations bills that Congress passes each year. It is designed to supplement the information provided by the House and Senate Appropriations Subcommittees on Agriculture by summarizing the current legislative status of the bill, its scope, major issues, funding levels, and related legislative activity. The report also lists the key CRS staff relevant to the issues covered and related CRS products.
Date:
December 3, 2001
Creator:
Chite, Ralph M.
Object Type:
Report
System:
The UNT Digital Library
Emergency Funding for Agriculture: A Brief History of Supplemental Appropriations, FY1989-FY2006
From FY1989 through FY2006, 33 appropriations, authorization, or farm disaster acts added approximately $55.4 billion in supplemental funding for U.S. Department of Agriculture (USDA) programs. The two most recent supplemental appropriations were provided in response to Hurricanes Katrina and Rita in the Gulf of Mexico and in preparation for a possible U.S. outbreak of avian influenza. Some FY1989, the vast majority of the total supplemental funding has been paid directly to farmers, primarily through two mechanisms: "market loss payments" and crop disaster payments. This report includes the total annual funding additions in the 33 acts providing economic and farm disaster assistance through USDA programs since FY1989.
Date:
July 3, 2006
Creator:
Chite, Ralph M.
Object Type:
Report
System:
The UNT Digital Library
Defense Authorization and Appropriation Bills: FY1970-FY2006
This report is a research aid, which lists the DOD authorization bills (Table 1) and appropriations bills (Table 2). This report includes all the pertinent information on the passage of these bills through the legislative process: bill numbers, report numbers, dates reported and passed, recorded vote numbers and vote tallies, dates of passage of the conference reports with their numbers and votes, vetoes, substitutions, dates of final passage, and public law numbers. Table 3 shows real growth or decline in national defense funding for FY1940-FY2009. Table 4 gives a more detailed picture of both regular and supplemental defense appropriations from the 103rd Congress to the present (FY1993-FY2005). Table 5 shows the President’s DOD appropriations budget requests for FY1950-FY2005 vs. final amount enacted.
Date:
April 21, 2006
Creator:
Coipuram, Thomas, Jr.
Object Type:
Report
System:
The UNT Digital Library
Defense Authorization and Appropriations Bills: FY1970-FY2006
This report is a research aid, which lists the DOD authorization bills (Table 1) and appropriations bills (Table 2). This report includes all the pertinent information on the passage of these bills through the legislative process: bill numbers, report numbers, dates reported and passed, recorded vote numbers and vote tallies, dates of passage of the conference reports with their numbers and votes, vetoes, substitutions, dates of final passage, and public law numbers. Table 3 shows real growth or decline in national defense funding for FY1940-FY2009. Table 4 gives a more detailed picture of both regular and supplemental defense appropriations from the 103rd Congress to the present (FY1993-FY2005). Table 5 shows the President’s DOD appropriations budget requests for FY1950-FY2005 vs. final amount enacted.
Date:
June 13, 2006
Creator:
Coipuram, Thomas, Jr.
Object Type:
Report
System:
The UNT Digital Library
Defense Authorization and Appropriations Bills: FY1970-FY2006
This report is a research aid, which lists the DOD authorization bills (Table 1) and appropriations bills (Table 2). This report includes all the pertinent information on the passage of these bills through the legislative process: bill numbers, report numbers, dates reported and passed, recorded vote numbers and vote tallies, dates of passage of the conference reports with their numbers and votes, vetoes, substitutions, dates of final passage, and public law numbers. Table 3 shows real growth or decline in national defense funding for FY1940-FY2009. Table 4 gives a more detailed picture of both regular and supplemental defense appropriations from the 103rd Congress to the present (FY1993-FY2005). Table 5 shows the President’s DOD appropriations budget requests for FY1950-FY2005 vs. final amount enacted.
Date:
June 13, 2006
Creator:
Coipuram, Thomas, Jr.
Object Type:
Report
System:
The UNT Digital Library
Water Infrastructure Financing: History of EPA Appropriations
This report summarizes, in chronological order, congressional activity to fund items in the State and Tribal Assistance Grants (STAG) account since 1987. In appropriations legislation, funding for EPA (Environmental Protection Agency) wastewater assistance is contained in the measure providing funds for the Department of the Interior, Environment, and Related Agencies.
Date:
January 19, 2006
Creator:
Copeland, Claudia
Object Type:
Report
System:
The UNT Digital Library
HIV/AIDS: Appropriations for Worldwide Programs in FY2001 and FY2002
This short report identifies FY2002 appropriations for worldwide HIV/AIDS efforts and includes tables comparing these appropriations with appropriations for FY2001.
Date:
March 19, 2002
Creator:
Copson, Raymond W.
Object Type:
Report
System:
The UNT Digital Library
HIV/AIDS International Programs: Appropriations, FY2002 - FY2004
This report summarizes the Administration's FY2004 request for international HIV/AIDS spending would commit $450 million to a new Global AIDS Initiative to be administered by the Department of State. This is the principal component of the President's Emergency Plan for AIDS Relief, announced in the State of the Union Message on January 28, 2003. The Administration is seeking $300 million for the President's Mother and Child HIV Prevention Initiative in FY2004, up from $200 million in the FY2003 request. The Senate-passed omnibus appropriations bill for FY2003 would provide $400 million for the Global Fund.
Date:
February 10, 2003
Creator:
Copson, Raymond W.
Object Type:
Report
System:
The UNT Digital Library
Pacific Northwest National Laboratory Annual Self-Evaluation Report: 2002
This report will summarize PNNL's progress toward accomplishment of the critical outcomes, objectives and performance indicators as delineated in the FY 2002 Performance Evaluation and Fee Agreement. In addition, this report will summarize PNNL's analysis of the results of the FY2002 Peer Reviews, the implementation of PNNL's FY2002 Operational Improvement Initiatives, and the resolution of the Key Areas for Improvements.
Date:
October 29, 2002
Creator:
Cuello, Robert
Object Type:
Report
System:
The UNT Digital Library
War on Drugs: The National Youth Anti-Drug Media Campaign
This report discusses the authorization of the National Youth Anti-Drug Media Campaign, a multi-media federal program to persuade America's youth not to use drugs, which expired at the end of FY2002. H.R. 2829 (passed by the House on March 13, 2006) and S. 2560 (reported by the Senate Judiciary Committee on May 25, 2006) would reauthorize the media campaign, along with the other programs run by the Office of National Drug Control Policy (ONDCP) during FY2007.
Date:
July 3, 2006
Creator:
Eddy, Mark
Object Type:
Report
System:
The UNT Digital Library
Tank Lay-Up Information Package and List of Questions for US Department of Energy High-Level Waste Tank Storage Sites
This document provides background information and a list of questions to be addressed during an information-gathering visit by Jacobs Engineering Group Inc personnel. Jacobs has been funded by the Tanks Focus Area to complete a task "Pre-closure Interim Tanks Maintenance." The overall objective of this task is to develop a central informaion center of site conditions, site requirements, alternative technical and other approaches, closure plans and activities, regulatory drivers and methodolgies for decision-making to assist site decisdion-makers in teh evaluation of alternative high-level waste (HLW) tank lay-up configureations. Lay-up is the term used for the period between intial decontamination and decommissioning of the tanks and final closure. Successful lay-up will place the tanks in a safe, stable, and minimum-maintenance mode until final closure.
Date:
June 21, 2002
Creator:
Elmore, Monte R. & Henderson, Colin
Object Type:
Report
System:
The UNT Digital Library
Pacific Northwest National Laboratory Institutional Plan FY 2002-2006
This Institutional Plan for FY 2002-2006 is the principal annual planning document submitted to the Department of Energy's Office of Science by Pacific Northwest National Laboratory in Richland, Washington. This plan describes the Laboratory's mission, roles, and technical capabilities in support of Department of Energy priorities, missions, and plans. It also describes the Laboratory strategic plan, key planning assumptions, major research initiatives, and program strategy for fundamental science, energy resources, environmental quality, and national security.
Date:
January 2, 2002
Creator:
Fisher, Darrell R. & Pearson, Erik W.
Object Type:
Report
System:
The UNT Digital Library