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The aim of this project was to develop Synthetic High-Affinity Ligands (SHALs), which bind with high affinity and specificity to proteins of interest for national security and cancer therapy applications. The aim of producing synthetic ligands for sensory devices as an alternative to antibody-based detection assays and therapeutic agents is to overcome the drawbacks associated with antibody-based in next-generation sensors and systems. The focus area of the project was the chemical synthesis of the SHALs. The project concentrated on two different protein targets. (a) The C fragment of tetanus and botulinum toxin, potential biowarfare agents. A SHAL for tetanus or botulinum toxin would be incorporated into a sensory device for the toxins. (b) HLA-DR10, a protein found in high abundance on the surface of Non-Hodgkins Lymphoma. A SHAL specific to a tumor marker, labeled with a radionuclide, would enable the targeted delivery of radiation therapy to metastatic disease. The technical approach used to develop a SHAL for each protein target will be described in more detail below. However, in general, the development of a SHAL requires a combination of computational modeling techniques, modern nuclear magnetic resonance spectroscopy (NMR) and synthetic chemistry.

The program is organized into three developmental periods. In Phase 1 the team will develop and demonstrate a proof-of-concept prototype design and develop a manufacturing plan to substantiate potential producibility at a target cost level of $800/kW factory manufacturing cost. Phase 2 will further develop the design and reduce the manufacturing cost to a level of $600 kW. Depending on an assessment of the maturity of the technology at the end of Phase 1, Phase 2 may be structured and supplemented to provide a limited production capability. Finally, in Phase 3, a full Value Package Introduction (VPI) Program will be integrated into the SECA program to develop a mass-producible design at a factory cost of $400/kW with full cross-functional support for unrestricted commercial sales. The path to market for new technology products in the Cummins system involves two processes. The first is called Product Preceding Technology, or PPT. The PPT process provides a methodology for exploring potentially attractive technologies and developing them to the point that they can be reliably scheduled into a new product development program with a manageable risk to the product introduction schedule or product quality. Once a technology has passed the PPT gate, it is available …

In 1999, Argonne National Laboratory-East (ANL-E) designed and installed a series of engineered plantings consisting of a vegetative cover system and approximately 800 hybrid poplars and willows rooting at various predetermined depths. The plants were installed using various methods including Applied Natural Science's TreeWell{reg_sign} system. The goal of the installation was to protect downgradient surface and groundwater by hydraulic control of the contaminated plume by intercepting the contaminated groundwater with the tree roots, removing moisture from the upgradient soil area, reducing water infiltration, preventing soil erosion, degrading and/or transpiring the residual volatile organic compounds (VOCs), and removing tritium from the subsoil and groundwater. This report presents the results of the monitoring activities conducted by Argonne's Energy Systems Division (ES) in the growing season of 2003. ES was tasked with the biomonitoring of the plantation to determine contaminant uptake and groundwater contact. VOCs were found in plant tissue both at the French Drain and the Hydraulic Control locations in varying concentrations, and tritium levels in transpirate was found to continue a trend of higher concentrations compared to the background in the ANL-E area.

This report is a compilation of the calendar year 2003 groundwater sampling results from the Area 5 Radioactive Waste Management Site, Nevada Test Site. Wells Ue5PW-1, Ue5PW-2, and Ue5PW-3 were sampled semi-annually for the required analytes: pH, specific conductance, total organic carbon (TOC), total organic halides (TOX), tritium, and major cations/anions. Results from all samples collected in 2003 were within established criteria. These data indicate that there has been no measurable impact to the uppermost aquifer from the Resource Conservation and Recovery Act (RCRA) regulated unit within the Area 5 Radioactive Waste Management Site and confirm that any previous detections of TOC and TOX were false positives. Contamination indicator data are presented in control chart and tabular form with investigation levels indicated. Gross water chemistry data are presented in graphical and tabular form. There were no major changes noted in the monitored groundwater elevations. There continues to be an extremely small gradient to the northeast with an average flow velocity of less than one foot per year. Other information in the report includes a Cumulative Chronology for the Area 5 Radioactive Waste Management Site Groundwater Monitoring Program, a brief description of the site hydrogeology, and the current groundwater sampling procedure.

This document describes the 2003 SNL ASCI Software Quality Engineering (SQE) assessment of twenty ASCI application code teams and the results of that assessment. The purpose of this assessment was to determine code team compliance with the Sandia National Laboratories ASCI Applications Software Quality Engineering Practices, Version 2.0 as part of an overall program assessment.

The 2003 Wastewater Land Application Site Performance Reports for the Idaho National Engineering and Environmental Laboratory describe the conditions for the facilities with State of Idaho Wastewater Land Application Permits. Permit-required monitoring data are summarized, and permit exceedences or environmental impacts relating to the operations of the facilities during the 2003 permit year are discussed.

We have proposed a technique that could reduce CO{sub 2} emissions from near coastal fossil-fuel power plants using existing power plant cooling water flow rates (Rau and Caldeira, 1999; Caldeira and Rau, 2000). Preliminary cost estimates are as low as $68 per tonne C sequestered, as compared to > $170 per tonne C estimated for other approaches to CO{sub 2} separation with geologic or deep-ocean storage. Engineers at McDermott Technologies, Inc., have independently estimated the cost of our proposed technique, and came to the conclusion that our cost estimates were at the high end of the likely range. Interest has been expressed in pursuing this approach further both in Norway and in Japan. We have proved the viability of our concept using (1) bench-top laboratory experiments (Figures 1 and 2), (2) computer modeling of those experiments, (3) more sophisticated cost estimates, and (4) three-dimensional computer modeling of the consequences to global ocean chemistry (Figure 3 and 4). The climate and environmental impacts of our current, carbon intensive energy usage demands that effective and practical energy alternatives and CO{sub 2} mitigation strategies be found. As part of this effort, various means of capturing and storing CO{sub 2} generated from fossil-fuel-based energy …

This Technical Memorandum (TM) summarizes the accelerator and experiment operations for the period FY 2001 through FY 2003. The plan is to have an annual TM to gather such information in one place. In this case, the information concerns the startup of Run II at the Tevatron Collider and the beginning of the MiniBooNE neutrino experiment. While the focus is on the FY 2003 efforts, this document includes summaries of the earlier years where available for completeness.

The Accurate Time-Linked data Acquisition System (ATLAS II) is a small, lightweight, time-synchronized, robust data acquisition system that is capable of acquiring simultaneous long-term time-series data from both a wind turbine rotor and ground-based instrumentation. This document is a user's manual for the ATLAS II hardware and software. It describes the hardware and software components of ATLAS II, and explains how to install and execute the software.

The report surveys the original legal landscape surrounding this exemption – in the contexts of determining applicability of New Source Performance Standards, and New Source Review in Prevention of Significant Deterioration and nonattainment areas. It then summarizes the many significant developments during the current Bush Administration, both in the Federal Register and in the courts.

The Directorate of Public Works Environmental & Natural Resources Division (Fort Stewart /Hunter Army Airfield) contracted with the Pacific Northwest National Laboratory (PNNL) to monitor particulate matter (PM) concentrations on Fort Stewart, Georgia. The purpose of this investigation was to establish a PM sampling network using monitoring equipment typically used in U.S. Environmental Protection Agency (EPA) ''saturation sampling'', to determine air quality on the installation. In this initial study, the emphasis was on training-generated PM, not receptor PM loading. The majority of PM samples were 24-hr filter-based samples with sampling frequency ranging from every other day, to once every six days synchronized with the EPA 6th day national sampling schedule. Eight measurement sites were established and used to determine spatial variability in PM concentrations and evaluate whether fluctuations in PM appear to result from training activities and forest management practices on the installation. Data collected to date indicate the average installation PM2.5 concentration is lower than that of nearby urban Savannah, Georgia. At three sites near the installation perimeter, analyses to segregate PM concentrations by direction of air flow across the installation boundary indicate that air (below 80 ft) leaving the installation contains less PM2.5 than that entering the installation. …

Colloids have the potential to transport strongly sorbing radionuclide contaminants in soils and groundwater aquifers. Recent studies from the Nevada Test Site have indicated the enhanced mobility plutonium, albeit in minute quantities, associated with various silicate minerals (Kersting et al., 1999); however, significant colloidal transport of thorium (Th) and rare earths (RE) in nature, considered to be chemical analogs for plutonium, is rare. Yet, the current Yucca Mountain model for colloids would have predicted extensive Th- and RE migration, given these phases' association with clay minerals. Several studies have pointed to the effect of water flow rate on colloid and particulate migration. In this paper, we examine the benefit of relating water flow rate and the wasteform alteration structure to colloid release.

This issue brief discusses the increasing attention being paid to alternative fuels and advanced technology vehicles, the proponents of which point to their potential to improve urban air quality, decrease dependence on foreign oil, and reduce greenhouse gas emissions. The brief also discusses the major barriers currently preventing widespread use of such technologies, and discusses these technologies in the particular contexts of the 2005 Gulf Coast hurricanes and the Energy Policy Act of 2005.

From Summary: This report is a section by section explanation of the effects of S. 1709, the SAFE Act, on current law.

This report presents the results of the 2003 Radiological Environmental Monitoring Program conducted in accordance with 10 CFR 72.44 for the Fort St. Vrain Independent Spent Fuel Storage Installation. A description of the facility and the monitoring program is provided. The results of monitoring the predominant radiation exposure pathway, direct radiation exposure, indicate the facility operation has not contributed to any increase in the estimated maximum potential dose commitment to the general public.

The anomalous skin effect in a plasma with a highly anisotropic electron velocity distribution function (EVDF) is very different from skin effect in a plasma with the isotropic EVDF. An analytical solution was derived for the electric field penetrated into plasma with the EVDF described as a Maxwellian with two temperatures Tx >> Tz, where x is the direction along the plasma boundary and z is the direction perpendicular to the plasma boundary. The skin layer was found to consist of two distinctive regions of width of order nTx/w and nTz/w, where nTx,z/w = (Tx,z/m)1/2 is the thermal electron velocity and w is the incident wave frequency.

An increase in photocurrent has been observed at silicon electrodes coated with nanostructured porous silica films as compared to bare, unmodified silicon. Ultimately, to utilize this effect in devices such as sensors or microchip power supplies, the physical phenomena behind this observation need to be well characterized. To this end, Electrochemical Impedance Spectroscopy (EIS) was used to characterize the effect of surfactant-templated mesoporous silica films deposited onto silicon electrodes on the electrical properties of the electrode space-charge region in an aqueous electrolyte solution, as the electrical properties of this space-charge region are responsible for the photobehavior of semiconductor devices. A significant shift in apparent flat-band potential was observed for electrodes modified with the silica film when compared to bare electrodes; the reliability of this data is suspect, however, due to contributions from surface states to the overall capacitance of the system. To assist in the interpretation of this EIS data, a series of measurements at Pt electrodes was performed with the hope of decoupling electrode and film contributions from the EIS spectra. Surprisingly, the frequency-dependent impedance data for Pt electrodes coated with a surfactant-templated film was nearly identical to that observed for bare Pt electrodes, indicating that the mesoporous film …

Frequency domain substructure synthesis is a modeling technique that enables the prediction of a combined response of individual structures using experimentally measured or numerically predicted frequency response functions (FRFs). The traditional synthesis algorithm [1,2] operates on component impedances and thus generally requires several matrix inversions. An improved algorithm, developed by Jetmundsen et al. [3], requires a single matrix inversion with a completely arbitrary interface definition that can easily incorporate connection impedances. The main limitations of the method are the large data requirements and sensitivity to data truncation. The utility of this technique is demonstrated through a comparison of synthesized and measured admittances of an edge-stiffened plate with attached equipment. The plate mobilities are obtained from a numerical analysis because of the ability to accurately model this structure using a finite element representation. The attachments are characterized experimentally because of their complexity. The sections describe the synthesis technique and show numerical and experimental results for the plate and equipment.

In this report we summarize findings from a study of the predicted performance of a suite of application codes taken from the research environment and analyzed against a modeling framework for the HTMT architecture. We find that the inward bandwidth of the data vortex may be a limiting factor for some applications. We also find that available memory in the cryogenic layer is a constraining factor in the partitioning of applications into parcels. The architecture in several examples may be inadequately exploited; in particular, applications typically did not capitalize well on the available computational power or data organizational capability in the PIM layers. The application suite provided significant examples of wide excursions from the accepted (if simplified) program execution model--in particular, by required complex in-SPELL synchronization between parcels. The availability of the HTMT-C emulation environment did not contribute significantly to the ability to analyze applications, because of the large gap between the available hardware descriptions and parameters in the modeling framework and the types of data that could be collected via HTMT-C emulation runs. Detailed analysis of application performance, and indeed further credible development of the HTMT-inspired program execution model and system architecture, requires development of much better tools. Chief …

In 2002, the Program for Climate Model Diagnosis and Intercomparison (PCMDI) proposed the concept for a state-of-the-science appraisal of climate models to be performed approximately every two years. Motivation for this idea arose from the perceived needs of the international modeling groups and the broader climate research community to document progress more frequently than provided by the Intergovernmental Panel on Climate Change (IPCC) Assessment Reports. A committee of external reviewers, which included senior researchers from four leading international modeling centers, supported the concept by stating in its review: ''The panel enthusiastically endorses the suggestion that PCMDI develop an independent appraisal of coupled model performance every 2-3 years. This would provide a useful 'mid-course' evaluation of modeling progress in the context of larger IPCC and national assessment activities, and should include both coupled and single-component model evaluations.''

This report is a guide to one of the 13 regular appropriations bills that Congress considers each year.

This report is a guide to one of the 13 regular appropriations bills that Congress considers each year.

An investigation of the dredging practices of various countries, the impact of dredging on cultural artifacts/resources, and recommendations for mitigation of adverse impacts on cultural resources when dredging in the Gulf of Mexico.