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Preliminary evaluation of the lifecycle costs and market barriers of reflective pavements (open access)

Preliminary evaluation of the lifecycle costs and market barriers of reflective pavements

The objective of this study is to evaluate the life cycle costs and market barriers associated with using reflective paving materials in streets and parking lots as a way to reduce the urban heat island effect. We calculated and compared the life cycle costs of conventional asphalt concrete (AC) pavements to those of other existing pavement technologies with higher reflectivity-portland cement concrete (PCC), porous pavements, resin pavements, AC pavements using light-colored chip seals, and AC pavements using light-colored asphalt emulsion additives. We found that for streets and parking lots, PCC can provide a cost-effective alternative to conventional AC when severely damaged pavements must be completely reconstructed. We also found that rehabilitating damaged AC streets and intersections with thin overlays of PCC (ultra-thin white topping) can often provide a cost-effective alternative to standard rehabilitation techniques using conventional AC. Chip sealing is a common maintenance treatment for low-volume streets which, when applied using light-colored chips, could provide a reflective pavement surface. If the incremental cost of using light-colored chips is low, this chip sealing method could also be cost-effective, but the incremental costs of light-colored chips are as of yet uncertain and expected to vary. Porous pavements were found to have higher …
Date: November 21, 2001
Creator: Ting, M.; Koomey, J.G. & Pomerantz, M.
Object Type: Report
System: The UNT Digital Library
Explicit Determination of Piezoelectric Eshelby Tensors for a Spheroidal Inclusion (open access)

Explicit Determination of Piezoelectric Eshelby Tensors for a Spheroidal Inclusion

In this paper, by systematically treating the integrals involved in the piezoelectric inclusion problem, explicit results were obtained for the piezoelectric Eshelby tensors for a spheroidal inclusion aligned along the axis of the anisotropy in a transversely isotropic piezoelectric material. This problem was first treated by Dunn and Wienecke (1996) using a Green's function approach, which closely follows Withers' approach (1989) for an ellipsoidal inclusion problem in a transversely isotropic elastic medium. The same problem was recently treated by Michelitsch and Levin (2000) also using a Green's function approach. In this paper, a different method was used to obtain the explicit results for the piezoelectric Eshelby tensors for a spheroidal inclusion. The method is a direct extension of a more unified approach, which has been recently developed by Mikata (2000), which is based on Deeg's results (1980) on a piezoelectric inclusion problem. The main advantage of this method is that it is more straightforward and simpler than Dunn and Wienecke (1996), or Michelitsch and Levin (2000), and the results are a little bit more explicit than their solutions. The key step of this paper is an analytical closed form evaluation of several integrals, which was made possible after a careful …
Date: June 21, 2001
Creator: Mikata, Yozo
Object Type: Report
System: The UNT Digital Library
Effects of composition and exposure on the solar reflectance of Portland cement concrete (open access)

Effects of composition and exposure on the solar reflectance of Portland cement concrete

Increasing the solar reflectance (albedo) of a paved surface keeps it cooler in the sun, reducing convection of heat from pavement to air and thereby decreasing the ambient air temperature. Simulations of the influence of pavement albedo on air temperature in Los Angeles predict that increasing the albedo of 1,250 km2 of pavement by 0.25 would save cooling energy worth $15M yr-1, and reduce smog-related medical and lost-work expenses by $76M yr-1. Most sidewalks and a small fraction of roads and parking areas are paved with portland cement concrete, which can be made quite reflective through suitable choice of cement and aggregate. Variations with composition and environmental exposure of the albedos of portland cement concrete pavements were investigated through laboratory fabrication and exposure of 32 mixes of concrete. Twenty-four mixes yielded substandard, ''rough'' concretes due to high, unmet aggregate water demand. The albedos of the remaining eight ''smooth'' concrete mixes ranged from 0.41 to 0.77 (mean 0.59). Simulated weathering, soiling, and abrasion each reduced average concrete albedo (mean decreases 0.06, 0.05, and 0.19, respectively), though some samples became slightly more reflective through weathering or soiling. Simulated rain (wetting) strongly depressed the albedos of concretes (mean decrease 0.23) until their surfaces …
Date: December 21, 2001
Creator: Levinson, Ronnen & Akbari, Hashem
Object Type: Report
System: The UNT Digital Library
Oak Ridge Multiple Attribute System (ORMAS) for Pu, HEU, HE, Chemical Agents, and Drugs (open access)

Oak Ridge Multiple Attribute System (ORMAS) for Pu, HEU, HE, Chemical Agents, and Drugs

The concept for the Oak Ridge Multiple Attribute System (ORMAS) is a Nuclear Materials Identification System (NMIS) time-dependent coincidence processor that incorporates gamma ray spectrometry and utilizes a small, lightweight, portable DT neutron (14.1 MeV) generator (1 x 10{sup 8} n/s), proton recoil scintillation detectors, and a gamma ray detector (HPGe). ORMAS is based on detecting fission neutrons and gamma rays from inherent source fission, fission induced by the external DT source, gamma ray detection of natural emissions of uranium and Pu, and induced gamma ray emission by the interaction of the 14.1 MeV neutrons from the DT source. This system is uniquely suited for detection of shielded highly enriched uranium (HEU), plutonium and other special nuclear materials, and detection of high explosives (HE), chemical agents, and in some cases, drugs. It could easily be adjusted to utilize a trusted processor that incorporates information barrier and authentication techniques using open software and then be useful in some international applications for materials whose characteristics may be classified. Since it is based entirely on commercially available components, the entire system, including the NMIS data acquisition boards, can be built with commercial off the shelf components (COTS). ORMAS incorporates the PINS technology of …
Date: September 21, 2001
Creator: Mihalczo, J.T.
Object Type: Report
System: The UNT Digital Library
Modeling the acceleration field and objective lens for an aberration corrected photoemission electron microscope (open access)

Modeling the acceleration field and objective lens for an aberration corrected photoemission electron microscope

None
Date: September 21, 2001
Creator: Feng, J.; Padmore, H.; Wei, D.H.; Anders, S.; Wu, Y.; Scholl, A. et al.
Object Type: Article
System: The UNT Digital Library
A Measurement System for Systematic Hydrological Characterization of Unsaturated Fractured Welded Tuff in a Mined Underground Tunnel (open access)

A Measurement System for Systematic Hydrological Characterization of Unsaturated Fractured Welded Tuff in a Mined Underground Tunnel

A field investigation of unsaturated flow through a lithophysal unit of fractured welded tuff containing lithophysal cavities has been initiated. To characterize flow in this spatially heterogeneous medium, a systematic approach has been developed to perform tests in boreholes drilled at regular intervals in an underground tunnel (drift). In this paper, we describe the test equipment system that has been built for this purpose. Since the field-scale measurements, of liquid flow in the unsaturated, fractured rocks, require continuous testing for periods of days to weeks, the control of test equipment has been fully automated, allowing operation with no human presence at the field site. Preliminary results from the first set of tests are described. These tests give insight into the role of the matrix (perhaps also lithophysal cavities) as potential storage during the initial transient flow prior to the breakthrough of water at the drift crown, as well as the role of connected fractures that provide the subsequent quasi-steady flow. These tests also reveal the impact of evaporation on seepage into the drift.
Date: November 21, 2001
Creator: Cook, P. J.; Salve, R.; Freifeld, B. M. & Tsang, Y. W.
Object Type: Report
System: The UNT Digital Library

Commission to Strengthen Social Security

On May 2, President Bush announced establishment of a bipartisan, 16-member Commission "to study and report specific recommendations to preserve Social Security for seniors while building wealth for younger Americans." The Commission will provide bipartisan recommendations to the President for modernizing and restoring fiscal soundness to the Social Security System according to the following principles: modernization must not change Social Security benefits for retirees or near retirees; the entire Social Security surplus must be dedicated to Social Security only; Social Security payroll taxes must not be increased; the Government must not invest Social Security funds in the stock market; modernization must preserve Social Security's disability and survivors components; and modernization must include individually controlled, voluntary personal retirement accounts, which will augment the Social Security safety net.
Date: May 21, 2001
Creator: President's Commission to Strengthen Social Security
Object Type: Website
System: The UNT Digital Library
Supporting Rock Fall Calculation for Drift Degradation: Quantification of Uncertainties (open access)

Supporting Rock Fall Calculation for Drift Degradation: Quantification of Uncertainties

The purpose of this calculation is to quantify previously unquantified uncertainties associated with the Drift Degradation Analysis (BSC 2001b). The results from this calculation will support the development of a technical report to document supplemental science and performance analyses. This calculation has been developed according to the guidance provided by the ''Technical Work Plan for EBS Department Modeling FY 01 Work Activities'' (BSC 2001a). The specific objective of this calculation is to perform sensitivity studies to assess the impact of the following parameters on rock fall results: (1) parameter M, a multiplier of fracture trace lengths sampled from field data; (2) Terzaghi correction factor for subhorizontal fractures; and (3) number of Monte Carlo simulations. The scope of this calculation includes using the fracture data inputs from the ''Drift Degradation Analysis'' (BSC 2001b) as input to the Discrete Region Key Block Analysis (DRKBA) numerical code. Outputs from this program includes the expected quantities and size distributions of failed rock blocks. This calculation is applicable to unsupported drifts without backfill. This calculation is associated with the engineering and performance analysis activities of the Engineered Barrier System Department, and was developed in accordance with AP-3.12Q, ''Calculations''.
Date: November 21, 2001
Creator: Lin, M. & Kicker, D. C.
Object Type: Report
System: The UNT Digital Library
MULTISCALE THERMOHYDROLOGIC MODEL (open access)

MULTISCALE THERMOHYDROLOGIC MODEL

The purpose of the Multiscale Thermohydrologic Model (MSTHM) is to describe the thermohydrologic evolution of the near-field environment (NFE) and engineered barrier system (EBS) throughout the potential high-level nuclear waste repository at Yucca Mountain for a particular engineering design (CRWMS M&O 2000c). The process-level model will provide thermohydrologic (TH) information and data (such as in-drift temperature, relative humidity, liquid saturation, etc.) for use in other technical products. This data is provided throughout the entire repository area as a function of time. The MSTHM couples the Smeared-heat-source Drift-scale Thermal-conduction (SDT), Line-average-heat-source Drift-scale Thermohydrologic (LDTH), Discrete-heat-source Drift-scale Thermal-conduction (DDT), and Smeared-heat-source Mountain-scale Thermal-conduction (SMT) submodels such that the flow of water and water vapor through partially-saturated fractured rock is considered. The MSTHM accounts for 3-D drift-scale and mountain-scale heat flow, repository-scale variability of stratigraphy and infiltration flux, and waste package (WP)-to-WP variability in heat output from WPs. All submodels use the nonisothermal unsaturated-saturated flow and transport (NUFT) simulation code. The MSTHM is implemented in several data-processing steps. The four major steps are: (1) submodel input-file preparation, (2) execution of the four submodel families with the use of the NUFT code, (3) execution of the multiscale thermohydrologic abstraction code (MSTHAC), and (4) binning …
Date: December 21, 2001
Creator: Buscheck, T.A.
Object Type: Report
System: The UNT Digital Library
An Integrated RELAP5-3D and Multiphase CFD Code System Utilizing a Semi Implicit Coupling Technique (open access)

An Integrated RELAP5-3D and Multiphase CFD Code System Utilizing a Semi Implicit Coupling Technique

An integrated code system consisting of RELAP5-3D and a multiphase CFD program has been created through the use of a generic semi-implicit coupling algorithm. Unlike previous CFD coupling work, this coupling scheme is numerically stable provided the material Courant limit is not violated in RELAP5-3D or at the coupling locations. The basis for the coupling scheme and details regarding the unique features associated with the application of this technique to a four-field CFD program are presented. Finally, the results of a verification problem are presented. The coupled code system is shown to yield accurate and numerically stable results.
Date: June 21, 2001
Creator: Aumiller, D. L.; Tomlinson, E. T. & Weaver, W. L.
Object Type: Article
System: The UNT Digital Library
17th Edition of TOP500 List of World's Fastest SupercomputersReseased (open access)

17th Edition of TOP500 List of World's Fastest SupercomputersReseased

17th Edition of TOP500 List of World's Fastest Supercomputers Released MANNHEIM, GERMANY; KNOXVILLE, TENN.; BERKELEY, CALIF. In what has become a much-anticipated event in the world of high-performance computing, the 17th edition of the TOP500 list of the world's fastest supercomputers was released today (June 21). The latest edition of the twice-yearly ranking finds IBM as the leader in the field, with 40 percent in terms of installed systems and 43 percent in terms of total performance of all the installed systems. In second place in terms of installed systems is Sun Microsystems with 16 percent, while Cray Inc. retained second place in terms of performance (13 percent). SGI Inc. was third both with respect to systems with 63 (12.6 percent) and performance (10.2 percent).
Date: June 21, 2001
Creator: Strohmaier, Erich; Meuer, Hans W.; Dongarra, Jack J. & Simon,Horst D.
Object Type: Report
System: The UNT Digital Library
Final report on the NuTeV Decay Channel (open access)

Final report on the NuTeV Decay Channel

A search for a 33.9 MeV/c weakly interacting neutral particle produced in pion decay is discussed along with other studies.
Date: March 21, 2001
Creator: Conrad, Janet M.
Object Type: Report
System: The UNT Digital Library
Isotopically controlled semiconductors (open access)

Isotopically controlled semiconductors

Semiconductor bulk crystals and multilayer structures with controlled isotopic composition have attracted much scientific and technical interest in the past few years. Isotopic composition affects a large number of physical properties, including phonon energies and lifetimes, bandgaps, the thermal conductivity and expansion coefficient and spin-related effects. Isotope superlattices are ideal media for self-diffusion studies. In combination with neutron transmutation doping, isotope control offers a novel approach to metal-insulator transition studies. Spintronics, quantum computing and nanoparticle science are emerging fields using isotope control.
Date: December 21, 2001
Creator: Haller, Eugene E.
Object Type: Article
System: The UNT Digital Library
Aspects of Coulomb dissociation and interference in peripheral nucleus-nucleus collisions (open access)

Aspects of Coulomb dissociation and interference in peripheral nucleus-nucleus collisions

Coherent vector meson production in peripheral nucleus-nucleus collisions is discussed. These interactions may occur for impact parameters much larger than the sum of the nuclear radii. Since the vector meson production is always localized to one of the nuclei, the system acts as a two-source interferometer in the transverse plane. By tagging the outgoing nuclei for Coulomb dissociation it is possible to obtain a measure of the impact parameter and thus the source separation in the interferometer. This is of particular interest since the life-time of the vector mesons are generally much shorter than the impact parameters of the collisions.
Date: October 21, 2001
Creator: Nystrand, Joakim; Baltz, Anthony & Klein, Spencer R.
Object Type: Article
System: The UNT Digital Library
Streamlined Approach for Environmental Restoration (SAFER) Plan for Corrective Action Unit 356: Mud Pits and Disposal Sites, Nevada Test Site, Nevada (Revision No. 0, August 2001) (open access)

Streamlined Approach for Environmental Restoration (SAFER) Plan for Corrective Action Unit 356: Mud Pits and Disposal Sites, Nevada Test Site, Nevada (Revision No. 0, August 2001)

This Streamlined Approach for Environmental Restoration (SAFER) Plan addresses the actions necessary for the characterization and closure of Corrective Action Unit (CAU) 356, Mud Pits and Disposal Sites, as identified in the Federal Facility Agreement and Consent Order (FFACO). The CAU, located on the Nevada Test Site in Nevada, consists of seven Corrective Action Sites (CASs): CAS 03-04-01, Area 3 Change House Septic System; CAS 03-09-01, Mud Pit Spill Over; CAS 03-09-03, Mud Pit; CAS 03-09-04, Mud Pit; CAS 03-09-05, Mud Pit; CAS 20-16-01, Landfill; CAS 20-22-21, Drums. Sufficient information and process knowledge from historical documentation and investigations are the basis for the development of the phased approach chosen to address the data collection activities prior to implementing the preferred closure alternative for each CAS. The Phase I investigation will determine through collection of environmental samples from targeted populations (i.e., mud/soil cuttings above textural discontinuity) if contaminants of potential concern (COPCs) are present in concentrations exceeding preliminary action levels (PALs) at each of the CASs. If COPCs are present above PALs, a Phase II investigation will be implemented to determine the extent of contamination to support the appropriate corrective action alternative to complete closure of the site. Groundwater impacts from …
Date: August 21, 2001
Creator: U.S. Department of Energy, National Nuclear Security Administration Nevada Operations Office (NNSA/NV)
Object Type: Report
System: The UNT Digital Library
Corrective Action Investigation Plan for Corrective Action Unit 168: Areas 25 and 26 Contaminated Materials and Waste Dumps, Nevada Test Site, Nevada (Rev. 0) includes Record of Technical Change No. 1 (dated 8/28/2002), Record of Technical Change No. 2 (dated 9/23/2002), and Record of Technical Change No. 3 (dated 6/2/2004) (open access)

Corrective Action Investigation Plan for Corrective Action Unit 168: Areas 25 and 26 Contaminated Materials and Waste Dumps, Nevada Test Site, Nevada (Rev. 0) includes Record of Technical Change No. 1 (dated 8/28/2002), Record of Technical Change No. 2 (dated 9/23/2002), and Record of Technical Change No. 3 (dated 6/2/2004)

This Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Operations Office's approach to collect data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit 168 under the Federal Facility Agreement and Consent Order. Corrective Action Unit 168 consists of a group of twelve relatively diverse Corrective Action Sites (CASs 25-16-01, Construction Waste Pile; 25-16-03, MX Construction Landfill; 25-19-02, Waste Disposal Site; 25-23-02, Radioactive Storage RR Cars; 25-23-18, Radioactive Material Storage; 25-34-01, NRDS Contaminated Bunker; 25-34-02, NRDS Contaminated Bunker; CAS 25-23-13, ETL - Lab Radioactive Contamination; 25-99-16, USW G3; 26-08-01, Waste Dump/Burn Pit; 26-17-01, Pluto Waste Holding Area; 26-19-02, Contaminated Waste Dump No.2). These CASs vary in terms of the sources and nature of potential contamination. The CASs are located and/or associated wit h the following Nevada Test Site (NTS) facilities within three areas. The first eight CASs were in operation between 1958 to 1984 in Area 25 include the Engine Maintenance, Assembly, and Disassembly Facility; the Missile Experiment Salvage Yard; the Reactor Maintenance, Assembly, and Disassembly Facility; the Radioactive Materials Storage Facility; and the Treatment Test Facility Building at Test Cell A. Secondly, the three CASs located in …
Date: November 21, 2001
Creator: U.S. Department of Energy, National Nuclear Security Administration Nevada
Object Type: Report
System: The UNT Digital Library
Adapting MARSSIM for FUSRAP site closure. (open access)

Adapting MARSSIM for FUSRAP site closure.

The Multi-Agency Radiation Survey and Site Investigation Manual (MARSSIM) provides a coherent, technically defensible process for establishing that exposed surfaces satisfy site cleanup requirements. Unfortunately, many sites have complications that challenge a direct application of MARSSIM. Example complications include Record of Decision (ROD) requirements that are not MARSSIM-friendly, the potential for subsurface contamination, and incomplete characterization information. These types of complications are typically the rule, rather than the exception, for sites undergoing radiologically-driven remediation and closure. One such site is the Formerly Utilized Sites Remedial Action Program (FUSRAP) Linde site in Tonawanda, New York. Cleanup of the site is currently underway. The Linde site presented a number of challenges to designing and implementing a closure strategy consistent with MARSSIM. This paper discusses some of the closure issues confronted by the U.S. Army Corps of Engineers Buffalo District at the Linde site, and describes how MARSSIM protocols were adapted to address these issues.
Date: December 21, 2001
Creator: Johnson, R.; Durham, L.; Rieman, C. & Hoover, R.
Object Type: Article
System: The UNT Digital Library
Synthesis of oxime-linked mucin mimics containing thetumor-related TN and sialyl TN antigens (open access)

Synthesis of oxime-linked mucin mimics containing thetumor-related TN and sialyl TN antigens

The synthesis of oxime-linked mucin mimics was accomplished via the incorporation of multiple ketone residues into a peptide followed by reaction with aminooxy sugars corresponding to the tumor-related T{sub N} and sialyl T{sub N} (ST{sub N}) antigens.
Date: August 21, 2001
Creator: Marcaurelle, Lisa A.; Shin, Youngsook; Goon, Scarlett & Bertozzi,Carolyn R.
Object Type: Article
System: The UNT Digital Library
Practical superconductor development for electrical power applications - Argonne National Laboratory - quarterly report for the period ending June 30, 2001. (open access)

Practical superconductor development for electrical power applications - Argonne National Laboratory - quarterly report for the period ending June 30, 2001.

This is a multiyear experimental research program focused on improving relevant material properties of high-T{sub c} superconductors (HTSs) and on development of fabrication methods that can be transferred to industry for production of commercial conductors. The development of teaming relationships through agreements with industrial partners is a key element of the Argonne (ANL) program.
Date: August 21, 2001
Creator: Dorris, S. E.
Object Type: Report
System: The UNT Digital Library
Review of D-T Experiments Relevant to Burning Plasma Issues (open access)

Review of D-T Experiments Relevant to Burning Plasma Issues

Progress in the performance of tokamak devices has enabled not only the production of significant bursts of fusion energy from deuterium-tritium (D-T) plasmas in the Tokamak Fusion Test Reactor (TFTR) and the Joint European Torus (JET) but, more importantly, the initial study of the physics of burning magnetically confined plasmas. The TFTR and JET, in conjunction with the worldwide fusion effort, have studied a broad range of topics including magnetohydrodynamic stability, transport, wave-particle interactions, the confinement of energetic particles, and plasma boundary interactions. The D-T experiments differ in three principal ways from previous experiments: isotope effects associated with the use of deuterium-tritium fuel, the presence of fusion-generated alpha particles, and technology issues associated with tritium handling and increased activation. The effect of deuterium-tritium fuel and the presence of alpha particles is reviewed and placed in the perspective of the much large r worldwide database using deuterium fuel and theoretical understanding. Both devices have contributed substantially to addressing the scientific and technical issues associated with burning plasmas. However, future burning plasma experiments will operate with larger ratios of alpha heating power to auxiliary power and will be able to access additional alpha-particle physics issues. The scientific opportunities for extending our understanding …
Date: December 21, 2001
Creator: Hawryluk, R.J.
Object Type: Report
System: The UNT Digital Library
The Particle Cleanliness Validation System (open access)

The Particle Cleanliness Validation System

The Particle Cleanliness Validation System (PCVS) is a combination of a surface particle collection tool and a microscope based data, reduction system for determining the particle cleanliness of mechanical and optical surfaces at LLNL. Livermore is currently constructing the National Ignition Facility (NIF), a large 192 beam laser system for studying fusion physics. The laser is entirely enclosed. in aluminum and stainless steel vessels containing several environments; air, argon, and vacuum. It contains uncoated optics as well as hard dielectric coated and softer solgel coated optics which are, to varying degrees, sensitive to opaque particles, translucent particles, and molecular contamination. To quantify the particulate matter on structural surfaces during vendor cleaning and installation, a novel instrument has been developed to-both collect surface particles and to quantify the number and size distribution of these particles. The particles are collected on membrane filter paper which is ''swiped'' on a test surface for a proscribed distance to collect sufficient particles to significantly exceed the cleanliness of the filter paper. The swipe paper is then placed into a cassette for protection from further. contamination and transported to a microscope with x-y motorized stage and image analysis software, The surface of the swipe paper is …
Date: December 21, 2001
Creator: Stowers, I.F. & Ravizza, D.L.
Object Type: Article
System: The UNT Digital Library
Actinide-Aluminate Speciation in Alkaline Radioactive Waste (open access)

Actinide-Aluminate Speciation in Alkaline Radioactive Waste

Investigation of behavior of actinides in alkaline media containing AL(III) showed that no aluminate complexes of actinides in oxidation states (IIII-VIII) were formed in alkaline solutions. At alkaline precipitation IPH (10-14) of actinides in presence of AL(III) formation of aluminate compounds is not observed. However, in precipitates contained actinides (IIV)<(VI), and to a lesser degree actinides (III), some interference of components takes place that is reflected in change of solid phase properties in comparison with pure components or their mechanical mixture. The interference decreases with rise of precipitation PH and at PH 14 is exhibited very feebly. In the case of NP(VII) the individual compound with AL(III) is obtained, however it is not aluminate of neptunium(VII), but neptunate of aluminium(III) similar to neptunates of other metals obtained earlier.
Date: December 21, 2001
Creator: Clark, Dr. David L. & Fedosseev, Dr. Alexander M.
Object Type: Report
System: The UNT Digital Library
Horizontal gene transfer as adaptive response to heavy metal stress in subsurface microbial communities. Final report for period October 15, 1997 - October 15, 2000 (open access)

Horizontal gene transfer as adaptive response to heavy metal stress in subsurface microbial communities. Final report for period October 15, 1997 - October 15, 2000

Horizontal gene transfer as adaptive response to heavy metal stress in the presence of heavy metal stress was evaluated in oligotrophic subsurface soil laboratory scale microcosms. Increasing levels of cadmium (10, 100 and 1000 mM) were applied and an E. coli donor was used to deliver the target plasmids, pMOL187 and pMOL222, which contained the czc and ncc operons, and the helper plasmid RP4. Plasmid transfer was evaluated through monitoring of the heavy metal resistance and presence of the genes. The interactive, clearly revealed, effect of biological and chemical external factors on the extent of plasmid-DNA propagation in microbial communities in contaminated soil environments was observed in this study. Additionally, P.putida LBJ 415 carrying a suicide construct was used to evaluate selective elimination of a plasmid donor.
Date: December 21, 2001
Creator: Smets, B. F.
Object Type: Report
System: The UNT Digital Library
Texas Register, Volume 26, Number 51, Pages 10449-10680, December 21, 2001 (open access)

Texas Register, Volume 26, Number 51, Pages 10449-10680, December 21, 2001

A weekly publication, the Texas Register serves as the journal of state agency rulemaking for Texas. Information published in the Texas Register includes proposed, adopted, withdrawn and emergency rule actions, notices of state agency review of agency rules, governor's appointments, attorney general opinions, and miscellaneous documents such as requests for proposals. After adoption, these rulemaking actions are codified into the Texas Administrative Code.
Date: December 21, 2001
Creator: Texas. Secretary of State.
Object Type: Journal/Magazine/Newsletter
System: The Portal to Texas History