Our goal is to develop robust DNA signatures for rapid and specific DNA-based detection platforms that can be employed by CBNP to detect a wide range of potential agents. Our approach has resulted in highly specific DNA signatures for Yersina pestis, Bacillus anthracis and Brucella species. Furthermore, this approach can be applied to any genome (even uncharacterized ones), which facilitates DNA signature development for detection of newly emerging pathogens. We are using suppression subtractive hybridization (SSH) as a tool to define large DNA regions specific to multiple biothreat pathogens by comparing them to genomes of the most closely related organisms. This approach has become increasingly accurate as we continue to find new, distinctive strains and ever-closer near-neighbors. With the huge costs incurred by whole genome sequencing, it is not possible to sequence each new bacterial genome. However, it is completely practical to identify genome differences in the laboratory using SSH, and becomes especially useful when comparing new strains to previously sequenced genomes.

Appropriations are one part of a complex federal budget process that includes budget resolutions, appropriations (regular, supplemental, and continuing) bills, rescissions, and budget reconciliation bills. This report is a guide to one of the 13 regular appropriations bills that Congress passes each year. It is designed to supplement the information provided by the House and Senate Appropriations Subcommittees on Energy and Water.

This report describes the development of an ultra-low power spread spectrum receiver based on a programmable surface acoustic wave (SAW) correlator. This work was funded under LDRD 02-26573, Ultra-Low Power Spread Spectrum Receiver. The approach taken in this project uses direct demodulation of a radio frequency (RF) signal from carrier frequency to data frequency. This approach was taken to reduce power consumption and size. The design is based on the technique of correlating the received RF signal with the preprogrammed spreading code. The system requirements, applications, design methodology, and testing results are all documented in the following pages.

Potential leak detection, monitoring, and mitigation techniques are being developed to support Hanford single-shell tank waste retrieval operations. In July and August 2001, Pacific Northwest National Laboratory demonstrated several of these technologies for CH2M HILL Hanford Group, Inc., at the Mock Tank Site in the 200 East Area. These subsurface air flow and extraction (SAFE) technologies use air injection and extraction wells to create an advective air flowfield beneath a tank. SAFE includes the following technologies: 1) leak detection--in-tank tracers, flowfield disturbance, radon displacement, and tank waste vapors; 2) leak monitoring--partitioning tracer method and reactive tracers; 3) leak mitigation--soil desiccation before and after leakage and in situ gaseous reduction; and 4) subsurface characterization--interfacial tracers. This report provides an overview of these technologies and discusses the FY 2001 demonstration activities at the Mock Tank Site, their results, and implications for future work.

This short report identifies FY2002 appropriations for worldwide HIV/AIDS efforts and includes tables comparing these appropriations with appropriations for FY2001.

This report will summarize PNNL's progress toward accomplishment of the critical outcomes, objectives and performance indicators as delineated in the FY 2002 Performance Evaluation and Fee Agreement. In addition, this report will summarize PNNL's analysis of the results of the FY2002 Peer Reviews, the implementation of PNNL's FY2002 Operational Improvement Initiatives, and the resolution of the Key Areas for Improvements.

This document provides background information and a list of questions to be addressed during an information-gathering visit by Jacobs Engineering Group Inc personnel. Jacobs has been funded by the Tanks Focus Area to complete a task "Pre-closure Interim Tanks Maintenance." The overall objective of this task is to develop a central informaion center of site conditions, site requirements, alternative technical and other approaches, closure plans and activities, regulatory drivers and methodolgies for decision-making to assist site decisdion-makers in teh evaluation of alternative high-level waste (HLW) tank lay-up configureations. Lay-up is the term used for the period between intial decontamination and decommissioning of the tanks and final closure. Successful lay-up will place the tanks in a safe, stable, and minimum-maintenance mode until final closure.

This Institutional Plan for FY 2002-2006 is the principal annual planning document submitted to the Department of Energy's Office of Science by Pacific Northwest National Laboratory in Richland, Washington. This plan describes the Laboratory's mission, roles, and technical capabilities in support of Department of Energy priorities, missions, and plans. It also describes the Laboratory strategic plan, key planning assumptions, major research initiatives, and program strategy for fundamental science, energy resources, environmental quality, and national security.

This report summarizes the earthquake activity on Hanford for FY 2002. Hanford Seismic Monitoring provides an uninterrupted collection of high-quality raw and processed seismic data from the Hanford Seismic Network for the U.S. Department of Energy and its contractors. Hanford Seismic Monitoring also locates and identifies sources of seismic activity and monitors changes in the historical pattern of seismic activity at the Hanford Site. The data are compiled, archived, and published for use by the Hanford Site for waste management, Natural Phenomena Hazards assessments, and engineering design and construction. In addition, the seismic monitoring organization works with the Hanford Site Emergency Services Organization to provide assistance in the event of a significant earthquake on the Hanford Site. The Hanford Seismic Network and the Eastern Washington Regional Network consist of 41 individual sensor sites and 15 radio relay sites maintained by the Hanford Seismic Monitoring staff. For the Hanford Seismic Network, there were 1,177 triggers during fiscal year 2002. Of these triggers, 553 were earthquakes. Two earthquakes were located in the Hanford Seismic Network area. Stratigraphically 13 occurred in the Columbia River basalt, 12 were earthquakes in the pre-basalt sediments, and 17 were earthquakes in the crystalline basement. Geographically, 13 earthquakes …

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PNNL estimated the FY2002 energy, environmental, and financial benefits (i.e., metrics) of the technologies and practices in the U.S. Department of Energy's (DOE's) Office of Building Technology, State and Community Programs (BTS). BTS uses the estimates of benefits as part of its annual budget request. This report includes an overview of the analytical approaches used to estimate energy savings for the FY2002 appropriated budget for BTS. The report also includes descriptions of key assumptions and the methodology that is used to calculate energy savings estimates for each BTS program.

Appropriations are one part of a complex federal budget process that includes budget resolutions, appropriations (regular, supplemental, and continuing) bills, rescissions, and budget reconciliation bills. This report is a guide to one of the 13 regular appropriations bills that Congress passes each year. It is designed to supplement the information provided by the House and Senate Labor, Health and Human Services, and Education Appropriations Subcommittees.

Appropriations are one part of a complex federal budget process that includes budget resolutions, appropriations (regular, supplemental, and continuing) bills, rescissions, and budget reconciliation bills. This report is a guide to one of the 13 regular appropriations bills that Congress passes each year. It is designed to supplement the information provided by the House and Senate Labor, Health and Human Services, and Education Appropriations Subcommittees.

This report briefly describes the budget sequestration process, including the ways in which a sequester could be avoided under the regular process, and then discusses in more detail various techniques that have been used since FY1991 to prevent a sequester by intervening in the regular process.

Appropriations are one part of a complex federal budget process that includes budget resolutions, appropriations (regular, supplemental, and continuing) bills, rescissions, and budget reconciliation bills. This report is a guide to one of the 13 regular appropriations bills that Congress passes each year. It is designed to supplement the information provided by the Subcommittees on Transportation of the House and Senate Committees on Appropriations.

The purpose of this collaborative Idaho National Engineering and Environmental Laboratory (INEEL) and Massachusetts Institute of Technology (MIT) Laboratory Directed Research and Development (LDRD) project is to investigate the suitability of lead or lead-bismuth cooled fast reactors for producing low-cost electricity as well as for actinide burning. The goal is to identify and analyze the key technical issues in core neutronics, materials, thermal-hydraulics, fuels, and economics associated with the development of this reactor concept. Work has been accomplished in four major areas of research: core neutronic design, plant engineering, material compatibility studies, and coolant activation. The publications derived from work on this project (since project inception) are listed in Appendix A. This is the third in a series of Annual Reports for this project, the others are also listed in Appendix A as FY-00 and FY-01 Annual Reports.

Four boreholes were drilled at the Immobilized Low-Activity Waste Disposal Site in April 2002. Three were completed as groundwater monitoring wells. This report documents the results of the drilling and data collected from the drilling.

Annual report for the Sabine River Compact Administration for 2002, including a summary of the meetings and work of the administration, reports from the hydrologic stations, fiscal revenues and expenditures, and accompanying notes.

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This report discusses President Bush's 2002 requests for new funding and additional authority to provide assistance to Colombia and six regional neighbors in a continuation of the Andean Regional Initiative (ARI) launched in 2001.

This report discusses President Bush's 2002 requests for new funding and additional authority to provide assistance to Colombia and six regional neighbors in a continuation of the Andean Regional Initiative (ARI) launched in 2001.

This report discusses President Bush's 2002 requests for new funding and additional authority to provide assistance to Colombia and six regional neighbors in a continuation of the Andean Regional Initiative (ARI) launched in 2001.

This report discusses President Bush's 2002 requests for new funding and additional authority to provide assistance to Colombia and six regional neighbors in a continuation of the Andean Regional Initiative (ARI) launched in 2001.

Annual report of the Texas Alcoholic Beverage Commission describing goals, activities, and accomplishments during fiscal year 2002.