Dantrolene is a drug that suppresses intracellular Ca2+ release from sarcoplasmic reticulum in normal skeletal muscle and is used as a therapeutic agent in individuals susceptible to malignant hyperthermia. Though its precise mechanism of action has not been elucidated, we have identified the N-terminal region (amino acids 1-1400) of the skeletal muscle isoform of the ryanodine receptor (RyR1), the primary Ca2+ release channel in sarcoplasmic reticulum, as a molecular target for dantrolene using the photoaffinity analog [3H]azidodantrolene(1). Here, we demonstrate that heterologously expressed RyR1 retains its capacity to be specifically labeled with [3H]azidodantrolene,indicating that muscle specific factors are not required for this ligand-receptor interaction. Synthetic domain peptides of RyR1, previously shown to affect RyR1 function in vitro and in vivo, were exploited as potential drug binding site mimics and used in photoaffinity labeling experiments. Only DP1 and DP1-2, peptide s containing the amino acid sequence corresponding to RyR1 residues 590-609, were specifically labeled by [3H]azidodantrolene. A monoclonal anti-RyR1 antibody which recognizes RyR1 and its 1400 amino acid N-terminal fragment, recognizes DP1 and DP1-2 in both Western blots and immunoprecipitation assays, and specifically inhibits [3H]azidodantrolene photolabeling of RyR1 and its N-terminal fragment in sarcoplasmic reticulum. Our results indicate that synthetic domain …

This report discusses projected agricultural imports and exports for FY2002, as well as legislation that deals with federal programs in support of agricultural exports and federal aid dedicated to farms and agricultural reform.

A chapter report issued by the General Accounting Office with an abstract that begins "Thousands of the Federal Aviation Administration (FAA) controllers will soon be eligible to retire because of extensive hiring in the 1980's to replace striking air traffic controllers. Although the exact number and timing of the controllers' departures has not been determined, attrition scenarios developed by both FAA and GAO indicate that the total attrition will grow substantially in both the short and long term. As a result, FAA will likely need to hire thousands of air traffic controllers in the next decade to met increasing traffic demands and to address the anticipated attrition of experienced controllers, predominately because of retirement. FAA has yet to developed a comprehensive human capital workforce strategy to address its impending controller needs. Rather, FAA's strategy for replacing controllers is generally to hire new controllers only when current, experienced controllers leave. This does not take into account the potential increases in future hiring and the time necessary to train replacements. In addition, there is uncertainty about the ability of FAA's new aptitude test to identify the best controller candidates. Further, FAA has not addressed the resources that may be needed at its …

Daily newspaper from Altus, Oklahoma that includes local, state, and national news along with advertising.

The purpose of this study is to quantify the flow and transport model uncertainty for the Central Nevada Test Area (CNTA). Six parameters were identified as uncertain, including the specified head boundary conditions used in the flow model, the spatial distribution of the underlying welded tuff unit, effective porosity, sorption coefficients, matrix diffusion coefficient, and the geochemical release function which describes nuclear glass dissolution. The parameter uncertainty was described by assigning prior statistical distributions for each of these parameters. Standard Monte Carlo techniques were used to sample from the parameter distributions to determine the full prediction uncertainty. Additional analysis is performed to determine the most cost-beneficial characterization activities. The maximum radius of the tritium and strontium-90 contaminant boundary was used as the output metric for evaluation of prediction uncertainty. The results indicate that combining all of the uncertainty in the parameters listed above propagates to a prediction uncertainty in the maximum radius of the contaminant boundary of 234 to 308 m and 234 to 302 m, for tritium and strontium-90, respectively. Although the uncertainty in the input parameters is large, the prediction uncertainty in the contaminant boundary is relatively small. The relatively small prediction uncertainty is primarily due to the …

Daily newspaper from Baytown, Texas that includes local, state, and national news along with advertising.

The KEK Accelerator Test Facility (ATF) damping ring is a prototype low emittance source for the NLC/JLC linear collider. To achieve the goal normalized vertical emittance {gamma}{var_epsilon}{sub {gamma}} = 20 nm-rad, magnet placement accuracy better than 30 mm must be achieved. Accurate beam-based alignment (BBA) is required. The ATF arc optics uses a FOBO cell with two horizontally focusing quadrupoles, two sextupoles and a horizontally defocusing gradient dipole, all of which must be aligned with BBA. BBA at ATF uses the quadrupole and sextupole trim windings to find the trajectory through the center of each magnet. The results can be interpreted to assess the accuracy of the mechanical alignment and the beam position monitor offsets.

Semiweekly newspaper from Boerne, Texas that includes local, state, and national news along with advertising.

Semiweekly newspaper from Brady, Texas that includes local, state, and national news along with advertising.

This report discusses the budget for fiscal year 2002. The congress debates issues such as budget action, receipts surpluses or deficits, and the economy

A letter report issued by the General Accounting Office with an abstract that begins "The Balanced Budget and Emergency Deficit Control Act of 1985 requires that the Office of Management and Budget (OMB) and the Congressional Budget Office (CBO) issue sequestration reports annually to Congress. Overall, GAO found that OMB and CBO substantially complied with the act in fiscal year 2002. However, as in previous years, some of the required OMB and CBO reports were issued late. Further, GAO identified a total of 19 items where differences of over $500 million existed between CBO's and OMB's scoring of discretionary budget authority and/or outlays for enacted laws."

A new Detonation Profile Test (DPT) was developed to measure simultaneously the detonation wave breakout profile and the average detonation velocity at the breakout surface. The test evaluated small cylindrical samples with diameter up to 19.08 mm and length up to 33 mm. The experiment involved initiating a LX-17 cylindrical specimen and recording the wave breakout using a fast streaking electronic camera. The initiation was done using a PBX-9407 pellet (1.630 g/cm{sup 3}), which has a Chapman-Jouguet (C-J) pressure close to that of LX-17. The acceptor breakout surface had a 2 mm wide by 1 mm deep groove that provided a step in the recorded breakout profile for velocity determination. A 532-nm laser light illuminated the specimen surface. A streak camera looking perpendicular to the groove, recorded the extinction of the laser light as the detonation wave emerged from the surface. This technique provided a high-resolution spatial and temporal profile of the wave curvature as well as accurate timing of the propagating wave over the last millimeter of the sample. The measured groove depth and recorded travel time were then used to calculate the average detonation wave velocity. Results for 12.7 mm diameter unconfined LX-17 charges showed detonation velocity in …

Semi-weekly newspaper from Clifton, Texas that includes local, state, and national news along with advertising.

On August 7, 2002, President-elect, Alvaro Uribe Velez, age 49, is scheduled to take office amid an intensifying conflict. Uribe’s election has been widely attributed to his law-and-order campaign promises to pursue the guerrillas vigorously by increasing Colombia’s military budget, doubling the size of the military to 100,000, and creating a one-million man civilian militia to aid the Colombian military, as well as to the worsening security situation in Colombia.

This report provides information about the Country-of-Origin Labeling for Foods. Federal law requires most imports, including many food items to bear labels informing the "ultimate purchaser" of their country of origin.

Federal law requires most imports, including many food items, to bear labels informing the “ultimate purchaser” of their country of origin. Meats, produce, and several other raw agricultural products generally have been exempt. The omnibus farm law (P.L. 107-171) signed on May 13, 2002, contains a requirement that many retailers provide, starting on September 30, 2004, country-of-origin labeling (COOL) on fresh fruits and vegetables, red meats, seafood, and peanuts. The program is voluntary until then. USDA on October 8, 2002, issued guidelines for the voluntary labeling program.

Daily newspaper from Perry, Oklahoma that includes local, state, and national news along with advertising.

Weekly newspaper from Dallas, Texas that includes local, state, and national news and advertising of interest to the Lesbian, Gay, Bisexual, and Transgender (LGBT) community.

A clipping displaying the DEPOSIPAC trademark by Harland International Company of a total deposit of $320.00.

Report submitted by an American Libraries Association (ALA) Government Documents Round Table (GODORT) ad hoc committee charged to "create a report advising GODORT on the best practices and procedures in the digital library field and advise GODORT on the most effective organizational structure for support of the government information community in pursuing digital library initiatives" (p. 2).

A chapter report issued by the General Accounting Office with an abstract that begins "The Federal Energy Regulatory Commission (FERC) has not yet adequately revised its regulatory and oversight approach to respond to the transition to competitive energy markets. FERC recognizes that the transition from highly regulated monopolies to competitive markets requires a fundamental change in how it does business. However, it has struggled through various strategic planning and other efforts to define the specific strategies, processes, and activities to regulate and oversee these markets. GAO found that FERC (1) has had difficulty recruiting staff because it has trouble competing with private sector salaries; (2) faces the retirement of more than one-quarter of its employees by 2005; (3) has used recruitment bonuses, retention allowances, tuition reimbursement, and flexible work schedules to attract new staff and to retain current employees but has not taken advantage of the full range of personnel flexibilities and tools available to federal agencies, such as special salary rates; and (4) has not developed a strategic human capital management plan to assess its workforce needs and to develop strategies to address them."

Daily newspaper from Chickasha, Oklahoma that includes local, state, and national news along with advertising.

Funeral program for Sister Murina Jewell Collins, born February 24, 1935 and died June 7, 2002. The funeral was held June 14, 2002 at West End Baptist Church, officiated by Dr. A. B. Devers, I. The funeral arrangements were made through Lewis Funeral Home and she was buried in Meadowlawn Memorial Park in San Antonio, Texas.

Weekly newspaper from Grandview, Texas that includes local, state, and national news along with advertising.