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Internal Revenue Service: Assessment of Budget Request for Fiscal Year 2003 and Interim Results of 2002 Tax Filing Season
Testimony issued by the General Accounting Office with an abstract that begins "This testimony discusses the Internal Revenue Service (IRS) fiscal year 2003 budget request for the 2002 tax filing season. GAO found that IRS's plans for hiring and redirecting staff may be optimistic because budgets are prepared so far in advance of the fiscal year involved. IRS assumed (1) labor and nonlabor savings of 2,287 staff years and $157.5 million and (2) additional savings of $38.5 million from better business practices. IRS's justification does not always adequately link the resources being requested and the agency's performance goals. Although IRS provided adequate support to justify the $450 million request for its multiyear capital account for business systems modernization, it did not adequately support $1.63 billion of the $1.68 billion requested for its information systems. In the area of agency performance, GAO found that IRS has generally processed returns smoothly and seen continued growth in electronic filing. The one exception to smooth processing has been the large number of errors related to the rate reduction credit. IRS has had to correct millions of returns due to the credit, and taxpayers' call about the credit have greatly increased the demand on IRS's …
Date:
April 9, 2002
Creator:
United States. General Accounting Office.
Object Type:
Text
System:
The UNT Digital Library
Food Safety: Continued Vigilance Needed to Ensure Safety of School Meals
Testimony issued by the General Accounting Office with an abstract that begins "The national school lunch and breakfast programs provide inexpensive or free meals to more than 27 million children each day. During the 1990s, nearly 300 outbreaks of foodborne illness at the nation's schools sickened 16,000 students. The rise in the number of school outbreaks mirrors a rise in the number of outbreaks in the overall population, according to the Centers for Disease Control and Prevention (CDC). Because the CDC data include outbreaks attributable to food brought from home or other sources, GAO could not determine the extent to which food served in the school meal programs caused reported outbreaks. Data from 1998 and 1999 do show, however, that most of the outbreaks during those years were caused by foods served through the school meal program. Foods contaminated with salmonella and Norwalk-like viruses were the most common causes of outbreaks. GAO found that the Department of Agriculture has not developed security measures to protect foods served at schools from deliberate contamination. The existing food safety system is a patchwork of protections that fall short in addressing existing and emerging food safety threats."
Date:
April 30, 2002
Creator:
United States. General Accounting Office.
Object Type:
Text
System:
The UNT Digital Library
Federal Real Property: Views on Real Property Reform Issues
Testimony issued by the General Accounting Office with an abstract that begins "The Federal Property Asset Management Reform Act of 2002, will enhance federal real and personal property management and bring the policies and business practices of federal agencies into the 21st century. Available data show that the federal government owns hundreds of thousands of properties worldwide, including military installations, office buildings, laboratories, courthouses, embassies, postal facilities, national parks, forests, and other public lands, estimated to be worth billions of dollars. Most of this government-owned real property is under the custody and control of eight agencies--the Department of Agriculture, Defense, Energy, the Interior, and Veterans Affairs; General Services Administration; the Tennessee Valley Authority; and the U.S. Postal Service. Federal property managers have a large deferred maintenance backlog, obsolete and underutilized properties, and changing facility needs due to rapid advances in technology. It is important that real property-holding agencies link their real property strategic plans to their missions and related capital management and performance plans; ensure that senior real property officers have the knowledge, skills, and expertise needed to effectively perform their duties; are accountable for the reliability, usefulness, and timeliness of their data; and adopt an effective process to monitor …
Date:
April 18, 2002
Creator:
United States. General Accounting Office.
Object Type:
Text
System:
The UNT Digital Library
Military Personnel: Active Duty Benefits Reflect Changing Demographics, but Continued Focus Is Needed
Testimony issued by the General Accounting Office with an abstract that begins "Since the advent of the all-volunteer Army in 1973, the number of servicemembers with spouses and children has grown significantly. Although the Department of Defense (DOD) has responded positively to this change, GAO believes that some current family benefits could be strengthened. For example, despite improvements to the quality of military child care centers, DOD needs to further expand child care capacity. Moreover, DOD faces challenges in increasing awareness and use of these benefits. GAO compared the military's benefits with those of the private sector, including retirement pay, health care, life insurance, and paid time-off, and found no significant gaps in the benefits offered to active duty servicemen and women. In fact, some military benefits exceed those offered by the private sector."
Date:
April 11, 2002
Creator:
United States. General Accounting Office.
Object Type:
Text
System:
The UNT Digital Library
Drinking Water Infrastructure: Information on Estimated Needs and Financial Assistance
Testimony issued by the General Accounting Office with an abstract that begins "The Environmental Protection Agency (EPA) conducts an infrastructure needs assessment every four years to estimate the future capital investment needs of local drinking water systems. In its most recent survey, EPA estimated that nearly $151 billion will be needed during the next 20 years to repair, replace, and upgrade the nation's 55,000 community water systems. The needs assessment survey serves as the basis for EPA's grants to the states under the drinking water revolving fund program. To ensure that it collected valid data to estimate drinking water infrastructure needs EPA conducted site visits to selected systems and had states review supporting documentation. However, EPA cannot tell how closely the estimates reflect actual state-by-state needs because it did not calculate the precision of estimates. GAO found that 31 out of 50 states surveyed established revolving loan funds programs to assist disadvantaged communities. In fiscal years 1991 through 2000, nine federal agencies made available about $44 billion in grants, loans, and loan guarantees for drinking water and wastewater capital improvements."
Date:
April 11, 2002
Creator:
United States. General Accounting Office.
Object Type:
Text
System:
The UNT Digital Library
Combating Terrorism: Intergovernmental Cooperation in the Development of a National Strategy to Enhance State and Local Preparedness
Testimony issued by the General Accounting Office with an abstract that begins "Federal, state, and local governments share responsibility for terrorist attacks. However, local government, including police and fire departments, emergency medical personnel, and public health agencies, is typically the first responder to an incident. The federal government historically has provided leadership, training, and funding assistance. In the aftermath of September 11, for instance, one-quarter of the $40 billion Emergency Response Fund was earmarked for homeland security, including enhancing state and local government preparedness. Because the national security threat is diffuse and the challenge is highly intergovernmental, national policymakers must formulate strategies with a firm understanding of the interests, capacity, and challenges facing those governments. The development of a national strategy will improve national preparedness and enhance partnerships between federal, state, and local governments. The creation of the Office of Homeland Security is an important and potentially significant first step. The Office of Homeland Security's strategic plan should (1) define and clarify the appropriate roles and responsibilities of federal, state, and local entities; (2) establish goals and performance measures to guide the nation's preparedness efforts; and (3) carefully choose the most appropriate tools of government to implement the national strategy …
Date:
April 2, 2002
Creator:
United States. General Accounting Office.
Object Type:
Text
System:
The UNT Digital Library
Education Financial Management: Weak Internal Controls Led to Instances of Fraud and Other Improper Payments
Testimony issued by the General Accounting Office with an abstract that begins "The Department of Education has a history of financial management problems, including serious internal control weaknesses, that have affected the Department's ability to provide reliable financial information on its operations. GAO found that significant internal control weaknesses in payment processes and poor physical control over its computer assets led to fraud, improper payments, and lost assets. GAO also identified instances of grant and loan fraud and pervasive control breakdowns and improper payments in other areas, particularly involving purchasing cards."
Date:
April 10, 2002
Creator:
United States. General Accounting Office.
Object Type:
Text
System:
The UNT Digital Library
Paperwork Reduction Act: Burden Increases and Violations Persist
Testimony issued by the General Accounting Office with an abstract that begins "This testimony discusses the implementation of the Paperwork Reduction Act of 1995 and information collection authorizations from the Office of Management and Budget (OMB) that either expired or were otherwise inconsistent with the act. GAO found that federal paperwork rose by 290 million burden hours during fiscal year 2001--the largest one-year increase since the act was amended and recodified in 1995. This occurred largely because the Internal Revenue Service (IRS) increased its paperwork estimate by about 250 million burden hours during the year. Most of the paperwork increase at IRS resulted from changes made by the agency--not because of new statutes. Federal agencies providing information to OMB identified more than 400 violations of the act during fiscal year 2001. Some of these violations have been going on for years, and they collectively represent substantial opportunity costs."
Date:
April 11, 2002
Creator:
United States. General Accounting Office.
Object Type:
Text
System:
The UNT Digital Library
Internal Revenue Service: Enhanced Efforts to Combat Abusive Tax Schemes--Challenges Remain
Testimony issued by the General Accounting Office with an abstract that begins "Estimating the extent of abusive tax schemes used by individual taxpayers is difficult because they are often hidden. Nevertheless, the Internal Revenue Service (IRS) believes that the number and dollar consequences of these schemes has grown recently. IRS estimates that 740,000 taxpayers used abusive schemes in tax year 2000. IRS caught $5 billion in improper tax avoidance or tax credit and refund claims, but estimates that another $20 to $40 billion went undetected. Recent developments suggest that the number of individuals using an abusive tax scheme involving offshore accounts may be greater than estimated and potential lost revenues may be higher than estimated. Because no one individual or office could provide an agencywide perspective on IRS's strategy, goals, objectives, performance measures, or program results, it is difficult to provide a clear picture of IRS's efforts to address abusive tax schemes. IRS has created new offices, reemphasized and reorganized earlier efforts, and plans to assign at least 200 additional staff to its efforts. Limited data suggest that IRS's enhanced efforts have helped to successfully convict those promoting and taking advantage of abusive schemes, publicize these results, and uncover previously …
Date:
April 11, 2002
Creator:
United States. General Accounting Office.
Object Type:
Text
System:
The UNT Digital Library
Welfare Reform: DOT Has Made Progress in Implementing the Job Access Program but Has Not Evaluated the Impact
Testimony issued by the General Accounting Office with an abstract that begins "The Department of Transportation's (DOT) Job Access and Reverse Commute (Job Access) Program, has presented implementation challenges for the Federal Transit Administration (FTA). In November 1999, DOT's process for selecting Job Access grantees was inconsistent and the basis for some selections was unclear. In response, DOT took steps to improve its process for selecting grantees by developing a standard format for reviewing proposals and providing more detailed guidance to its reviewers. Grantees reported problems in meeting standard grant requirements for obtaining Job Access funding. Half of the respondents GAO surveyed said that it took too long to satisfy standard FTA grant requirements--on average, about nine months from the time an applicant had been selected for a grant until the time the applicant had satisfied the requirements and received its grant. The Transportation Equity Act for the 21st Century required DOT to evaluate the Job Access program and issue a report by June 2000. However, DOT reportedly has no estimated date for issuing the report. DOT developed a plan and selected an increase in access to employment sites as the sole measure of program success. The use of employment …
Date:
April 17, 2002
Creator:
United States. General Accounting Office.
Object Type:
Text
System:
The UNT Digital Library
Veterans' Benefits: Despite Recent Improvements, Meeting Claims Processing Goals Will Be Challenging
Testimony issued by the General Accounting Office with an abstract that begins "The Department of Veterans Affairs (VA) will provide $25 billion in compensation and pension benefits in fiscal year 2002 to more than three million veterans, dependents and survivors. For years, the compensation and pension claims process has been subject to long long waits for decision and large claims backlogs. VA's goal for fiscal year 2003 is to complete accurate decisions on rating-related claims in an average of 100 days. To achieve this, the Veterans Benefits Administration (VBA) is focusing on increasing production of rating decisions and reducing the inventory of claims to about 250,000. As of the end of March 2002, VBA was completing claims in an average of 224 days and had an inventory of about 412,000 claims. VBA is trying to significantly increase regional offices' rating decision production to reduce the inventory, and, in turn, reduce the time required to complete decisions. VBA expects to increase production by hiring more staff and increasing the proficiency of new staff. Although VBA has recently increased its production and reduced its inventory, meeting its production and inventory reduction and its timeliness goals will be challenging."
Date:
April 26, 2002
Creator:
United States. General Accounting Office.
Object Type:
Text
System:
The UNT Digital Library
U.S. Government Financial Statements: FY 2001 Results Highlight the Continuing Need to Accelerate Federal Financial Management Reform
Testimony issued by the General Accounting Office with an abstract that begins "As in the past four years, GAO was unable to express an opinion on the federal government's consolidated financial statements for fiscal years 2000 and 2001 because of material weaknesses in internal control and accounting and reporting issues. These conditions prevented GAO from providing Congress and American citizens with an opinion as to whether the consolidated financial statements are fairly stated in conformity with U.S. generally accepted accounting principles. Until these problems are adequately addressed, the government will continue to have difficulty (1) reporting its assets, liabilities, and costs; (2) adequately measuring the full cost and financial performance of programs and effectively manage related operations; and (3) adequately safeguarding significant assets and and properly recording transactions."
Date:
April 9, 2002
Creator:
United States. General Accounting Office.
Object Type:
Text
System:
The UNT Digital Library
Medicare: Financial Outlook Poses Challenges for Sustaining Program and Adding Drug Coverage
Testimony issued by the General Accounting Office with an abstract that begins "The lack of outpatient prescription drug coverage may leave Medicare's most vulnerable beneficiaries with high out-of-pocket costs. Recent estimates suggest that, at any given time, more than a third of Medicare beneficiaries lack prescription drug coverage. The rest have some coverage through various sources--most commonly employer-sponsored health plans. Recent evidence indicates that this coverage is beginning to erode. The short- and long-term cost pressures facing Medicare will require substantial financing and programmatic reforms to put future Medicare on a sustainable footing. In the absence of a drug benefit, many Medicare beneficiaries obtain coverage through health plans, public programs, and the Medigap insurance market. The price, availability, and level of such coverage varies widely, leaving substantial gaps and exposure to high out-of-pocket costs for thousands. Despite pressures to adopt a prescription drug benefit, the rapidly rising cost of current obligations argues for careful deliberation and extreme caution in expanding benefits. GAO's long-term simulations show that the aging of the baby boomers and rising per capita health care spending will, absent meaningful reform, lead to massive fiscal challenges in future years."
Date:
April 17, 2002
Creator:
United States. General Accounting Office.
Object Type:
Text
System:
The UNT Digital Library
Land Management Agencies: Restoring Fish Passage Through Culverts on Forest Service and BLM Lands in Oregon and Washington Could Take Decades
Testimony issued by the General Accounting Office with an abstract that begins "GAO discussed the condition of culverts on fish-bearing streams in Oregon and Washington and the federal efforts to identify and restore culverts that are impeding fish passage on Bureau of Land Management (BLM) and Forest Service lands. This testimony is based on a November 2001 report (GAO-02-136), which described (1) the number of culverts that may impede fish passage on BLM and Forest Service lands in Oregon and Washington, (2) the factors affecting the agencies' ability to restore passage through culverts acting as barrier culverts, and (3) the results of the agencies' efforts to restore fish passage."
Date:
April 10, 2002
Creator:
United States. General Accounting Office.
Object Type:
Text
System:
The UNT Digital Library
Tax Administration: IRS Continues to Face Management Challenges in its Business Practices and Modernization Efforts
Testimony issued by the General Accounting Office with an abstract that begins "The Internal Revenue Service (IRS) was, for the second consecutive year, able to prepare financial statements that received an unqualified opinion. However, this achievement once again came through the use of substantial, costly, and time-consuming processes to compensate for serious systems and control deficiencies. IRS continues to make progress in its performance management system by using a strategic planning and budgeting process to reconcile competing priorities and initiatives with available resources. In the area of computer security, IRS corrected or mitigated many of the previously reported weaknesses, including those affecting its electronic filing systems. It also is implementing a computer security program that should help manage its risks in this area. Business Systems Modernization is IRS' ongoing program to leverage information technology and is integral to IRS achieving its customer-focused vision. To date, IRS has made progress in establishing the systems infrastructure, delivering system applications, and developing the management controls and capabilities necessary to effectively acquire and deploy modernized systems."
Date:
April 15, 2002
Creator:
United States. General Accounting Office.
Object Type:
Text
System:
The UNT Digital Library
Environmental Health Risks: Information on EPA's Draft Reassessment of Dioxins
A letter report issued by the General Accounting Office with an abstract that begins "Dioxins--chemical compounds that share structural and biological characteristics--have been linked to human illnesses, including cancer. Often the byproducts of combustion and industrial processes, complex mixtures of dioxins enter the food chain and human diet through emissions into the air. The Environmental Protection Agency (EPA) and the World Health Organization (WHO) noted the potential human health risks of dioxins in the 1970s when animal studies showed them to be among the most potent cancer-causing chemicals. EPA derived its estimates of human dietary exposure to dioxins in the United States from (1) chemically analyzed samples of 10 food types, (2) toxicity estimates of levels of individual dioxins in these foods, and (3) estimates of the quantities of these foods consumed by Americans. To develop more reliable national estimates of dietary exposure, EPA incorporated into its analysis some food studies that were nationally representative. Although both EPA and the WHO have assessed the human health risks of dioxins during the last decade, some of their objectives and processes have differed. Nonetheless, the analytical methods used and the conclusions reached have much in common. A major difference in the assessments …
Date:
April 26, 2002
Creator:
United States. General Accounting Office.
Object Type:
Report
System:
The UNT Digital Library
Welfare Reform: Interim Report on Potential Ways to Strengthen Federal Oversight of State and Local Contracting
A letter report issued by the General Accounting Office with an abstract that begins "The Personal Responsibility and Work Opportunity Reconciliation Act (PRWORA) of 1996 changed the nation's cash assistance program for needy families with children by replacing the Aid to Families with Dependent Children program, with the Temporary Assistance for Needy Families (TANF) block grant. As specified in PRWORA, TANF's goals include ending the dependence of families receiving government benefits by promoting job preparation, work, and marriage; preventing and reducing the incidence of non-marital pregnancies; and encouraging two-parent families. Contracting with nongovernmental entities to provide TANF-funded services occurs in most states and exceeded $1.5 billion in federal and state funds in 2001. A GAO survey indicated that the most commonly contracted services included education and training, job placement, and support services to promote job entry or retention. The Department of Health and Human Services (HHS) relies primarily on state single audit reports to oversee TANF contracting by states and localities. HHS officials told GAO that their regional offices follow up on the TANF deficiencies identified and that HHS focuses on reported deficiencies that involve unallowable or questionable costs. However, HHS officials said that they do not know the extent …
Date:
April 23, 2002
Creator:
United States. General Accounting Office.
Object Type:
Report
System:
The UNT Digital Library
Customs and INS: Information on Inspection, Infrastructure, Traffic Flow, and Security Matters at the Detroit Port of Entry
Correspondence issued by the General Accounting Office with an abstract that begins "Concerns have been raised about the effects of heightened security at two border crossings in Detroit--the Ambassador Bridge and the Detroit-Windsor Tunnel. In the wake of September 11, the U.S. Customs Service and the Immigration and Naturalization Service (INS) increased the number and thoroughness of inspections and questioned all bridge and tunnel travelers, leading to truck wait times that exceeded 10 hours. To decrease wait times, Customs and INS extended shifts, curtailed vacations, brought in more staff, and obtained help from the National Guard and local law enforcement. The U.S. and Canadian governments have agreed in principle to a plan to make the border more secure without impeding traffic flow and trade. In addition to increasing personnel and improving technology, officials are considering pre-approval of low-risk traffic and reverse inspections."
Date:
April 22, 2002
Creator:
United States. General Accounting Office.
Object Type:
Text
System:
The UNT Digital Library
NASA: Compliance With Cost Limits Cannot Be Verified
Correspondence issued by the General Accounting Office with an abstract that begins "The National Aeronautics and Space Administration's (NASA) authorization act for fiscal year 2000 limits expenditures for space station development to $25 billion and for shuttle launches to $17.7 billion. The act also requires NASA to (1) account for and report amounts obligated to date against the cost limits, (2) identify the amounts needed for future development and completion of the space station, and (3) arrange for GAO to verify the accounting within 60 days after submission of the budget request. Last year, GAO reported that NASA, as part of its fiscal year 2002 budget request, did not comply with the act's requirement to use obligations as its basis for reporting against the cost limits but instead used budget authority. The agency was also unable to provide detailed support for the amounts obligated against the limits for evaluation within the 60 days, but said that it could have provided the information if given more time. After a protracted effort, NASA has acknowledged that its systems cannot provide the data needed to support amounts obligated against the limits. NASA's inability to provide detailed data is due to its lack of …
Date:
April 10, 2002
Creator:
United States. General Accounting Office.
Object Type:
Text
System:
The UNT Digital Library
Power Systems Development Facility Gasification Test Run TC07
This report discusses Test Campaign TC07 of the Kellogg Brown & Root, Inc. (KBR) Transport Reactor train with a Siemens Westinghouse Power Corporation (Siemens Westinghouse) particle filter system at the Power Systems Development Facility (PSDF) located in Wilsonville, Alabama. The Transport Reactor is an advanced circulating fluidized-bed reactor designed to operate as either a combustor or a gasifier using a particulate control device (PCD). The Transport Reactor was operated as a pressurized gasifier during TC07. Prior to TC07, the Transport Reactor was modified to allow operations as an oxygen-blown gasifier. Test Run TC07 was started on December 11, 2001, and the sand circulation tests (TC07A) were completed on December 14, 2001. The coal-feed tests (TC07B-D) were started on January 17, 2002 and completed on April 5, 2002. Due to operational difficulties with the reactor, the unit was taken offline several times. The reactor temperature was varied between 1,700 and 1,780 F at pressures from 200 to 240 psig. In TC07, 679 hours of solid circulation and 442 hours of coal feed, 398 hours with PRB coal and 44 hours with coal from the Calumet mine, and 33 hours of coke breeze feed were attained. Reactor operations were problematic due to …
Date:
April 5, 2002
Creator:
Southern Company Services
Object Type:
Report
System:
The UNT Digital Library
Natural analogue synthesis report, TDR-NBS-GS-000027 rev00 icn02
The purpose of this report is to present analogue studies and literature reviews designed to provide qualitative and quantitative information to test and provide added confidence in process models abstracted for performance assessment (PA) and model predictions pertinent to PA. This report provides updates to studies presented in the Yucca Mountain Site Description (CRWMS M&O 2000 [151945], Section 13) and new examples gleaned from the literature, along with results of quantitative studies conducted specifically for the Yucca Mountain Site Characterization Project (YMP). The intent of the natural analogue studies was to collect corroborative evidence from analogues to demonstrate additional understanding of processes expected to occur during postclosure at a potential Yucca Mountain repository. The report focuses on key processes by providing observations and analyses of natural and anthropogenic (human-induced) systems to improve understanding and confidence in the operation of these processes under conditions similar to those that could occur in a nuclear waste repository. The process models include those that represent both engineered and natural barrier processes. A second purpose of this report is to document the various applications of natural analogues to geologic repository programs, focusing primarily on the way analogues have been used by the YMP. This report …
Date:
April 1, 2002
Creator:
Simmons, A.; Nieder-Westermann, G.; Stuckless, J.; Dobson, P.; Unger, A.J.A.; Kwicklis, E. et al.
Object Type:
Report
System:
The UNT Digital Library
Environmentally Assisted Cracking in Light Water Reactors : Semiannual Report, July 2000 - December 2000.
This report summarizes work performed by Argonne National Laboratory on fatigue and environmentally assisted cracking (EAC) in light water reactors (LWRs) from July 2000 to December 2000. Topics that have been investigated include (a) environmental effects on fatigue S-N behavior of primary pressure boundary materials, (b) irradiation-assisted stress corrosion cracking (IASCC) of austenitic stainless steels (SSs), and (c) EAC of Alloys 600 and 690. The fatigue strain-vs.-life data are summarized for the effects of various material, loading, and environmental parameters on the fatigue lives of carbon and low-alloy steels and austenitic SSs. Effects of the reactor coolant environment on the mechanism of fatigue crack initiation are discussed. Two methods for incorporating the effects of LWR coolant environments into the ASME Code fatigue evaluations are presented. Slow-strain-rate tensile tests and posttest fractographic analyses were conducted on several model SS alloys irradiated to {approx}0.9 x 10{sup 21} n {center_dot} cm{sup -2} (E > 1 MeV) in He at 289 C in the Halden reactor. The results were used to determine the influence of alloying and impurity elements on the susceptibility of these steels to IASCC. A fracture toughness J-R curve test was conducted on a commercial heat of Type 304 SS that …
Date:
April 1, 2002
Creator:
Chopra, O. K.; Chung, H. M.; Gruber, E. E.; Shack, W. J.; Soppet, W. K.; Strain, R. V. et al.
Object Type:
Report
System:
The UNT Digital Library
Development of Low Cost Total Energy Exchange Devices for Reducing Building Energy Consumption
The primary goal of this program is to develop a commercially viable enthalpy exchangers that exhibits performance comparable to state-of-the-art exchangers, meets flammability test standards, supports no biological growth, maintains mechanical integrity for a 10 year life (typical of competitive warrantees) and exhibits a total media raw material cost of approximately 1/5 of that of the competition. The first three program tasks were devoted to: (1) gathering background information on competitors and their methods of manufacturing; (2) establishing experimental and evaluation procedures for the program; (3) identifying potential desiccants, supports and binders to be tested; (4) preparing matrix combinations on a laboratory scale; (5) testing component combinations for equilibrium moisture uptakes at different relative humidities (isotherms) and dynamic moisture adsorption rates; (6) using media adsorption properties to models to predict exchanger performance; (7) estimating ease of manufacture and finished wheel costs; and (8) calculating raw material, wheel and cassette costs based on the best media formulations. Since the rotary enthalpy exchangers have high rotational speeds, uptake rate is at least as important as equilibrium moisture uptake. Therefore, a procedure and apparatus were developed to determine adsorption rate so that the Lewis number (the ratio of mass transfer resistance to heat …
Date:
April 22, 2002
Creator:
Belding, William; Collier, R. & Worek, William
Object Type:
Report
System:
The UNT Digital Library
Disruption of NBS1 gene leads to early embryonic lethality in homozygous null mice and induces specific cancer in heterozygous mice
Nijmegen breakage syndrome (NBS) is a rare autosomal recessive chromosome instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition, with cellular features similar to that of ataxia telangiectasia (AT). NBS results from mutations in the mammalian gene Nbs1 that codes for a 95-kDa protein called nibrin, NBS1, or p95. To establish an animal model for NBS, we attempted to generate NBS1 knockout mice. However, NBS1 gene knockouts were lethal at an early embryonic stage. NBS1 homozygous(-/-) blastocyst cells cultured in vitro showed retarded growth and subsequently underwent growth arrest within 5 days of culture. Apoptosis, assayed by TUNEL staining, was observed in NBSI homozygous(-/-) blastocyst cells cultured for four days. NBSI heterozygous(+/-) mice were normal, and exhibited no specific phenotype for at least one year. However, fibroblast cells from NBSI heterozygous(+/-) mice displayed an enhanced frequency of spontaneous transformation to anchorage-independent growth as compared to NBS1 wild-type(+/+) cells. Furthermore, heterozygous(+/-) mice exhibited a high incidence of hepatocellular carcinoma after one year compared to wild-type mice, even though no significant differences in the incidence of other tumors such as lung adenocarcinoma and lymphoma were observed. Taken together, these results strongly suggest that NBS1 heterozygosity and reduced NBSI expression induces …
Date:
April 15, 2002
Creator:
Kurimasa, Akihiro; Burma, Sandeep; Henrie, Melinda; Ouyang, Honghai; Osaki, Mitsuhiko; Ito, Hisao et al.
Object Type:
Report
System:
The UNT Digital Library