For many applications, silver salt-based autometallography (often also called silver enhancement or silver development) is required to visualize colloidal gold (1-5 nm in diameter) or the small 1.4 nm Nanogold{reg_sign} particles (Nanoprobes, Yaphank, NY, USA). Although even Nanogold may be seen directly by scanning-transmission electron microscopy (STEM), by transmission EM (TEM; in thin sections without stain or ice-embedded cryo-EM samples), energy filtered TEM, and scanning EM (SEM), silver enhancement makes viewing in the EM more facile since the particles are enlarged to approximately 10 to 20 nm, convenient for most specimens. Autometallographic (AMG) enhancement is required in order to visualize smaller gold particles such as Nanogold for light microscopy (LM) or in blots or gels. This chapter includes the following protocols: Protocol for HQ silver enhancement of Nanogold; Protocols for use of silver-enhanced Nanogold with osmium tetroxide--(A) Procedure using reduced concentration of OsO{sub 4}; (B) Procedures for gold toning; Protocol for HQ silver enhancement of Nanogold in pre-embedding immunocytochemistry for cell cultures; Protocol for gold enhancement of Nanogold for EM; Protocol for gold enhancement of Nanogold for LM; Protocol for staining blots with Nanogold and silver enhancement; and Protocol for staining gels with Nanogold and silver enhancement.

It is peculiar that in situ hybridization (ISH), a technique with many similarities to immunohistochemistry (IHC), has not enjoyed the phenomenal growth in both basic research and clinical applications as has its sister technique IHC. Since the late 1970s, when immunoperoxidase techniques began to be applied to routine diagnostic material and to numerous research applications, there has been a natural evolution of the IHC procedure. Namely, only a few primary antibodies were available commercially at the onset, and only one indirect and the peroxidase-antiperoxidase (PAP) technique detection systems were in place. With the advent of avidin-biotin detection systems and monoclonal antibodies, and a viable commercial market, extraordinary growth of the procedure's applications in clinical research and diagnostic pathology occurred during the subsequent two decades. Today, IHC is automated and widely used for research purposes and, to a large extent, has become a routine diagnostic ''special stain'' in most clinical laboratories. During the same period, ISH enjoyed very little growth in both research and diagnostic applications. What has accounted for this lack of maturation of the technique? The success of IHC is part of the reason measuring a gene's encoded protein routinely and inexpensively, particularly as automation evolved, rendered IHC a …

Interview with Navy veteran Frank Curre Jr. The interview includes Curre's personal experiences about joining the Navy, boot camp, being aboard the battleship USS Tennessee during the Japanese attack at Pearl Harbor, and his subsequent experiences in the Pacific Theater during World War II. Additionally, Curre talks about pre-war shipboard life and training exercises, his activities during the attack on Pearl Harbor, aftermath of the attack, his transfer to the yard minesweeper YMS-102 at Bremerton, Washington, operation around Midway Island, his transfer to the escort carrier Petrof Bay, the Battle of Leyte Gulf, kamikaze attacks, the Okinawa campaign, and continued combat against kamikazes.

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.

This is one of a series of sourcebooks on motor-driven equipment produced by the Industrial Technologies Program. It provides a reference for industrial fan systems users, outlining opportunities to improve fan system performance.

This study, done for the joint DOE-EPA Laboratories for the 21st Century program ("Labs 21"), used a simplified computer model to analyze the effects of energy efficiency measures in laboratory buildings in four different climates: those of Minneapolis, Denver, Seattle, and Atlanta. Results show that using variable-air-volume fume hoods can reduce lab energy costs as much as$1 per square foot in any climate. Energy-recovery systems such as enthalpy wheels also save varying amounts of energy and money in all climates. Savings for other measures, such as heat pipes and evaporated cooling, are also included.

This document provides information about mold and how to prevent it.

Transcript of an interview with Robert E. Yerger, a Navy veteran, concerning his experiences as a flying boat pilot in the Pacific Theater during World War II. Yerger discusses his decision to enlist in the Navy, May, 1942; basic flight training, Naval Air Station, Grand Prairie, Texas, 1942; advanced flight training, Naval Air Station, Corpus Christi, Texas, 1942; PBY flying boat training at Corpus Christi, 1942-43; his tenure as a flight instructor, Chase Field, Beeville, Texas, 1943; assignment as a base personnel officer at Chase Field, 1943-45; flying PB2Y flying boats with cargo and personnel in the Pacific, 1945; activities on Majuro Island; his experience flying the Martin Mars flying boat; mustering out of the service, August, 1946.

What happens when an expert on grief is faced with the slow decline of her beloved mother? Like A Grief Observed by C. S. Lewis, Singing Mother Home offers an inside look at the struggles of an “expert” in coping with loss. Donna S. Davenport was forced to rethink the traditional academic approach to the process, which implied that the goal of grief resolution was to end the attachment to the loved one. Instead, she embarked on a personal exploration of her own anticipatory grief. This intimate narrative forms the core of her book. It is emotionally wrenching, but it also provides hope for those going through similar experiences. Just as Davenport used her family's tradition of singing to comfort her mother, readers will be encouraged to find their own sources of comfort in family and legacy. The book concludes by describing psychological approaches to grief and recommending further reading. “This is a unique book by a professional who understands the field of loss and grief. . . . Poignantly heartbreaking.”--Melba Vasquez, President, American Psychology Association's Division on Counseling Psychology

Gold has been used for immunocytochemistry since 1971 when Faulk and Taylor discovered adsorption of antibodies to colloidal gold. It is an ideal label for electron microscopy (EM) due to its high atomic number, which scatters electrons efficiently, and the fact that preparative methods have been developed to make uniform particles in the appropriate size range of 5 to 30 nm. Use in light microscopy (LM) generally requires silver enhancement (autometallography; AMG) of these small gold particles. Significant advances in this field since that time have included a better understanding of the conditions for best antibody adsorption, more regular gold size production, adsorption of other useful molecules, like protein A, and advances in silver enhancement. Many studies have also been accomplished showing the usefulness of these techniques to cell biology and biomedical research. A further advance in this field was the development of Nanogold{trademark}, a 1.4 nm gold cluster. A significant difference from colloidal gold is that Nanogold is actually a coordination compound containing a gold core covalently linked to surface organic groups. These in turn may be covalently attached to antibodies. This approach to immunolabeling has several advantages compared to colloidal gold such as vastly better penetration into tissues, …

This booklet was prepared and distributed by the Brain Injury Association of Texas to answer the questions of brain injured persons' family, friends, and caretakers.

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.

This document provides information about vaccine and immunization requirements for children and students in the state of Texas.

This document is a review report noting 39 commendable practices and made 68 recommendations for improvements of the Marble Falls Independent School District(MFISD).

We review the phase diagram of nitrogen in a wide pressure and temperature range. Recent optical and x-ray diffraction studies at pressures up to 300 GPa and temperatures in excess of 1000 K have provided a wealth of information on the transformation of molecular nitrogen to a nonmolecular (polymeric) semiconducting and two new molecular phases. These newly found phases have very large stability (metastability) range. Moreover, two new molecular phases have considerably different orientational order from the previously known phases. In the iota phase (unlike most of other known molecular phases), N{sub 2} molecules are orientationally equivalent. The nitrogen molecules in the theta phase might be associated into larger aggregates, which is in line with theoretical predictions on polyatomic nitrogen.

Interview with community activist Gloria Villanueva-Anderson. The interview includes Villanueva-Anderson's personal experiences about being an activist in the Mexican-American community of Denton, Texas, education in Denton schools, discrimination at the train station in Denison, Texas, being accepted to the work-scholarship program of the FBI in 1952, opening her telephone answering exchange business, turning toward Republican politics, and her activities with George H.W. Bush's Texas Statewide Hispanic Campaign. Additionally, Villanueva-Anderson discusses her family background, the lack of discrimination against Hispanics in Denton, her family's assimilation in the Anglo culture, early Hispanic families in Denton, her appointment to the North Texas Hispanic Advisory Board by Senator John Tower, as well as her appointments to the Texas Small Business Task Force by Governor William Clements, the White House Conference on Small Business by President Jimmy Carter, and as Regional Advocate for the Small Business Administration by Ronald Reagan.

Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful technique for studying bi-molecular interactions at the atomic scale. Our NMR lab is involved in the identification of small molecules, or ligands that bind to target protein receptors, such as tetanus (TeNT) and botulinum (BoNT) neurotoxins, anthrax proteins and HLA-DR10 receptors on non-Hodgkin's lymphoma cancer cells. Once low affinity binders are identified, they can be linked together to produce multidentate synthetic high affinity ligands (SHALs) that have very high specificity for their target protein receptors. An important nanotechnology application for SHALs is their use in the development of robust chemical sensors or biochips for the detection of pathogen proteins in environmental samples or body fluids. Here, we describe a recently developed NMR competition assay based on transferred nuclear Overhauser effect spectroscopy (trNOESY) that enables the identification of sets of ligands that bind to the same site, or a different site, on the surface of TeNT fragment C (TetC) than a known ''marker'' ligand, doxorubicin. Using this assay, we can identify the optimal pairs of ligands to be linked together for creating detection reagents, as well as estimate the relative binding constants for ligands competing for the same site.

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.

Text describes the area, climate, agricultural history and statistics, oil-survey methods and definitions, soils and crops, land uses and agricultural methods, irrigation, and morphology and genesis of soils of Burleson County, Texas.