Unraveling Δ1-Pyrroline-5-Carboxylate-Proline Cycle in Plants by Uncoupled Expression of Proline Oxidation Enzymes (open access)

Unraveling Δ1-Pyrroline-5-Carboxylate-Proline Cycle in Plants by Uncoupled Expression of Proline Oxidation Enzymes

Article showing that overexpression of MsProDH in tobacco and Arabidopsis or impairment of P5C oxidation in the Arabidopsis p5cdh mutant did not change the cellular Pro to P5C ratio under ambient and osmotic stress conditions, indicating that P5C excess was reduced to Pro in a mitochondrial-cytosolic cycle. Results show that normal oxidation of P5C to Glu by P5CDH is key to prevent P5C-Pro intensive cycling and avoid ROS production from electron run-off.
Date: September 25, 2009
Creator: Miller, Gad; Honig, Arik; Stein, Hanan; Suzuki, Nobuhiro; Mittler, Ron & Zilberstein, Aviah
System: The UNT Digital Library
Mutations in Arabidopsis Fatty Acid Amide Hydrolase Reveal That Catalytic Activity Influences Growth but Not Sensitivity to Abscisic Acid or Pathogens (open access)

Mutations in Arabidopsis Fatty Acid Amide Hydrolase Reveal That Catalytic Activity Influences Growth but Not Sensitivity to Abscisic Acid or Pathogens

Article showing that the enhanced growth and NAE tolerance of the AtFAAH overexpressing seedlings depends on the catalytic activity of AtFAAH, hypersensitivity to ABA and hypersusceptibility to nonhost pathogens are independent of its enzymatic activity. Findings suggest that the AtFAAH influences plant growth and interacts with ABA signaling and plant defense through distinctly different mechanisms.
Date: December 4, 2009
Creator: Kim, Sang-Chul; Kang, Li; Nagaraj, Satish; Blancaflor, Elison B.; Mysore, Kirankumar S. & Chapman, Kent D.
System: The UNT Digital Library