Complete Genome Sequences of the Novel Cluster BP Phages Infecting Streptomyces sanglieri, AxeJC, Cumberbatch, Eastland, Eklok, HFrancette, Ignacio, Piccadilly, and Vondra (open access)

Complete Genome Sequences of the Novel Cluster BP Phages Infecting Streptomyces sanglieri, AxeJC, Cumberbatch, Eastland, Eklok, HFrancette, Ignacio, Piccadilly, and Vondra

Article describes the Streptomyces sanglieri bacteriophages AxeJC, Cumberbatch, Eastland,Eklok, HFrancette, Ignacio, Piccadilly, and Vondra form a novel actinobacteriophage cluster,BP. These siphoviruses have circularly permuted genomes with an average size of 37,700 bp and a GC content of 71%. Each genome contains approximately 58 protein-coding genes, with no tRNAs.
Date: September 14, 2022
Creator: Nath, Sreemoye; Sulaiman, Ahmad; Maneekul, Jindanuch; Bhuiyan, Swapan; Layton, Sonya R.; Menchaca, Carolina et al.
System: The UNT Digital Library
Developmental programming of DNA methylation and gene expression patterns is associated with extreme cardiovascular tolerance to anoxia in the common snapping turtle (open access)

Developmental programming of DNA methylation and gene expression patterns is associated with extreme cardiovascular tolerance to anoxia in the common snapping turtle

This article explores DNA methylation's role in the regulation of gene expression in reptiles. Results show that embryonic hypoxia programs DNA methylation and gene expression patterns and that these changes are associated with enhanced cardiac anoxia tolerance in snapping turtles later in life.
Date: September 6, 2021
Creator: Ruhr, Ilan; Bierstedt, Jacob; Rhen, Turk; Das, Debojyoti; Singh, Sunil Kumar; Miller, Solielle et al.
System: The UNT Digital Library
Dry blood spot samples to monitor immune-associated mRNA expression in intervention studies: Impact of Baker's yeast beta glucan (open access)

Dry blood spot samples to monitor immune-associated mRNA expression in intervention studies: Impact of Baker's yeast beta glucan

Article describes how monitoring immunological response to physical stressors in a field setting is challenging because existing methods require a laboratory visit and traditional blood collection via venipuncture. The purpose of this study was to determine if our optimized dry blood spot (DBS) methodology yields sufficient total RNA to quantify the effect of Baker's Yeast Beta Glucan supplementation on post-exercise mRNA expression.
Date: September 22, 2023
Creator: McFarlin, Brian K.; Bridgeman, Elizabeth A.; Vingren, Jakob L. & Will, David W.
System: The UNT Digital Library
Evaluation of pathways to the C-glycosyl isoflavone puerarin in roots of kudzu (Pueraria montana lobata) (open access)

Evaluation of pathways to the C-glycosyl isoflavone puerarin in roots of kudzu (Pueraria montana lobata)

Article describes how kudzu produces both O- and C-glycosylated isoflavones, with puerarin (C-glucosyl daidzein) as an important bioactive compound. The authors go on to explain how expression of recombinant UGTs in Escherichia coli and candidate C-glycosyltransferases in Medicago truncatula were used to explore substrate specificities, and gene silencing of UGT and key isoflavone biosynthetic genes in kudzu hairy roots employed to test hypotheses concerning the substrate(s) for C-glycosylation.
Date: September 2, 2022
Creator: Adolfo, Laci M.; Burks, David J.; Rao, Xiaolan; Alvarez-Hernandez, Anislay & Dixon, R. A.
System: The UNT Digital Library
A gene-editing/complementation strategy for tissue-specific lignin reduction while preserving biomass yield (open access)

A gene-editing/complementation strategy for tissue-specific lignin reduction while preserving biomass yield

This article develops a method, using a single DNA construct that uses CRISPR–Cas9 gene editing to knock-out expression of an endogenous gene of lignin monomer biosynthesis while at the same time expressing a modified version of the gene’s open reading frame that escapes cutting by the Cas9 system and complements the introduced mutation in a tissue-specific manner. This method is applicable to any plant species in which transformation and gene editing are feasible and validated vessel-specific promoters are available.
Date: September 3, 2021
Creator: Yu, Hasi; Liu, Chang & Dixon, R. A.
System: The UNT Digital Library
Habitat amount or landscape configuration: Emerging HotSpot analysis reveals the importance of habitat amount for a grassland bird in South Dakota (open access)

Habitat amount or landscape configuration: Emerging HotSpot analysis reveals the importance of habitat amount for a grassland bird in South Dakota

The authors of the article used pheasants (Phasianus colchicus) across South Dakota, USA as a model species to identify environmental factors driving spatiotemporal variation in population productivity. They found that the amount of grassland at a local spatial scale was the primary factor influencing an area being a HotSpot.
Date: September 26, 2022
Creator: Harsh, Sprih; Lonsinger, Robert C. & Gregory, Andrew
System: The UNT Digital Library
Metallic nanoparticle production and exposure/deposition system for toxicological research applications using zebrafish (open access)

Metallic nanoparticle production and exposure/deposition system for toxicological research applications using zebrafish

Article presents a method that provides a novel way to synthesize NPs and expose biological organisms for various toxicological analysis.
Date: September 3, 2020
Creator: Nayek, Subhayu; Aguilar, Roberto; Juel, Lauren A. & Verbeck, Guido F.
System: The UNT Digital Library
Multi-omic characterization of bifunctional peroxidase 4-coumarate 3-hydroxylase knockdown in Brachypodium distachyon provides insights into lignin modification associated pleiotropic effects (open access)

Multi-omic characterization of bifunctional peroxidase 4-coumarate 3-hydroxylase knockdown in Brachypodium distachyon provides insights into lignin modification associated pleiotropic effects

Article discusses how a bifunctional peroxidase enzyme, 4-coumarate 3-hydroxylase (C3H/APX), provides a parallel route to the shikimate shunt pathway for the conversion of 4-coumarate to caffeate in the early steps of lignin biosynthesis. Here, a multi-omic approach was used to characterize molecular changes resulting from C3H/APX-KD associated lignin modification and negative growth phenotype in Brachypodium distachyon.
Date: September 28, 2022
Creator: Shrestha, Him K.; Fichman, Yosef; Engle, Nancy L.; Tschaplinski, Timothy J.; Mittler, Ron; Dixon, R. A. et al.
System: The UNT Digital Library
Nest-site selection and breeding success of passerines in the world’s southernmost forests (open access)

Nest-site selection and breeding success of passerines in the world’s southernmost forests

This article studies the nest-site selection and survival rate of the five most abundant open-cup forest-dwelling passerines (Elaenia albiceps, Zonotrichia capensis, Phrygilus patagonicus, Turdus falcklandii, and Anairetes parulus) in the world’s southernmost forests of Navarino Island, in the Cape Horn Biosphere reserve, Chile (55°S).
Date: February 5, 2020
Creator: Jara Miller, Rocio F.; Crego, Ramiro D.; Samuel, Michael D.; Rozzi, Ricardo, 1960- & Jiménez, Jaime E.
System: The UNT Digital Library
Prostasin and hepatocyte growth factor B in factor VIIa generation: Serine protease knockdowns in zebrafish (open access)

Prostasin and hepatocyte growth factor B in factor VIIa generation: Serine protease knockdowns in zebrafish

Article aims to identify other proteases that may activate FVII using zebrafish as a model.
Date: September 22, 2020
Creator: Khandekar, Gauri; Iyer, Neha & Jagadeeswaran, Pudur
System: The UNT Digital Library
Silicon supply promotes differences in growth and C:N:P stoichiometry between bamboo and tree saplings (open access)

Silicon supply promotes differences in growth and C:N:P stoichiometry between bamboo and tree saplings

Article describes how silicon can be important for the growth, functioning, and stoichiometric regulation of nutrients for high-Si-accumulating bamboo. The authors demonstrated that silicon supply can decrease the ratio of C:Si in bamboo leaves and increase the ratio of N:P without altering nutrient status or the N:P ratio of tree spalings.
Date: September 21, 2023
Creator: Liu, Xiaoyu; Tang, Xinghao; Compson, Zacchaeus Greg; Huang, Dongmei; Zou, Guiwu; Luan, Fenggang et al.
System: The UNT Digital Library