Thermal Stress Has Minimal Effects on Bacterial Communities of Thermotolerant Symbiodinium Cultures (open access)

Thermal Stress Has Minimal Effects on Bacterial Communities of Thermotolerant Symbiodinium Cultures

Article comparing the microbial communities of three isolates from different species of the genus Symbiodinium (formerly known as Symbiodinium clade A) with different ecophysiology, levels of interaction with the animal host, and thermal adaptations. Results suggest that bacteria associated or co-cultured with thermotolerant Symbiodinium might play an important role in thermotolerance.
Date: May 19, 2022
Creator: Díaz-Almeyda, Erika M.; Ryba, Tyrone; Ohdera, Aki H.; Shafer, Natali; Link, Caroline; Prado-Zapata, Marcela et al.
System: The UNT Digital Library
RNaseH-mediated simultaneous piggyback knockdown of multiple genes in adult zebrafish (open access)

RNaseH-mediated simultaneous piggyback knockdown of multiple genes in adult zebrafish

This article tests if it is possible to piggyback more than one antisense deoxyoligonucleotide (dO) with one vivo morpholino (VMO). The authors previously developed a piggyback knockdown method that was used to knockdown genes in adult zebrafish. In this article, the authors develop a method to knockdown three genes at one time, and by increasing the concentration of VMO by twofold, could knockdown six genes simultaneously. These multiple gene knockdowns will not only increase the efficiency of the method in whole genome-wide knockdowns but will also be useful to study multifactorial disorders.
Date: November 19, 2020
Creator: Raman, Revathi; Ryon, Mia & Jagadeeswaran, Pudur
System: The UNT Digital Library
Metabolic and transcriptomic study of pennycress natural variation identifies targets for oil improvement (open access)

Metabolic and transcriptomic study of pennycress natural variation identifies targets for oil improvement

Article describes how, although pennycress, a winter annual, could be grown as a dedicated bioenergy crop, an increase in its seed oil content is required to improve its economic competitiveness. In this work, the authors combined biomass composition with metabolomic and transcriptomic studies of developing embryos from 22 pennycress natural variants to identify targets for oil improvement.
Date: June 19, 2023
Creator: Arias, Cintia Lucía; García Navarrete, Leidy Tatiana; Mukundi, Eric; Swanson, Tyler; Yang, Fan; Hernandez, Jonathan et al.
System: The UNT Digital Library
DNA metabarcoding captures different macroinvertebrate biodiversity than morphological identification approaches across a continental scale (open access)

DNA metabarcoding captures different macroinvertebrate biodiversity than morphological identification approaches across a continental scale

Article describes how DNA-based aquatic biomonitoring methods show promise to provide rapid, standardized, and efficient biodiversity assessment to supplement and in some cases replace current morphology-based approaches that are often less efficient and can produce inconsistent results. The authors present a comparison of DNA metabarcoding and morphological identification, leveraging national-scale, open-source, ecological datasets from the National Ecological Observatory Network.
Date: July 19, 2023
Creator: Emmons, Sean C.; Compson, Zacchaeus Greg; Malish, Megan C.; Busch, Michelle H.; Saenz, Veronica; Higgins, Kierstyn T. et al.
System: The UNT Digital Library