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Coming Out: The Lived Experiences of LGB College Students who Feel Supported by Their Parents (open access)

Coming Out: The Lived Experiences of LGB College Students who Feel Supported by Their Parents

The purpose of this phenomenological study was to explore how LGB college students created meaning out of their coming out process to their parents. I recruited LGB college students who perceived support from their parents during their coming out process and asked the following research question: What are the lived experiences of LGB college students who have experienced support from their parents during the coming out process? Seven White (n = 4), African American (n = 2), and Hispanic (n = 1) college students, three men and four women aged 18-24 years, shared narratives that included time periods before, during, and after their coming out disclosures to their parents. Using an adapted phenomenological analysis, I identified nine major themes: awareness of feeling different, positive relationship with parents prior to coming out, college impacting the coming out process, feeling unsure of how parents would respond to disclosure, parents assuring continued loved and acceptance, parents affirming LGB identity, increased relational depth with parents, increased sense of authenticity, and an appreciation for family's response and support. The findings provide insight into how counselors might work most beneficially with LGB college students and their parents around the coming out process. Opportunities for future research …
Date: May 2017
Creator: Price, Eric W.
System: The UNT Digital Library
A Community Based Assessment: An Analysis of Community Based Tourism Cooperatives in Kalache and Hulgol India (open access)

A Community Based Assessment: An Analysis of Community Based Tourism Cooperatives in Kalache and Hulgol India

This study incorporated a community based assessment with a focus on community based tourism in Kalache and Hulgol, India. Kalache and Hulgol are two agrarian based communities located in the environmentally significant region of the Western Ghats. Each of these communities has considered community based tourism as a means to reduce urban youth outmigration, to diversify economic resources, and to encourage the empowerment of women. The primary goals of this study were to understand the community issues and objectives, to determine the level of support for tourism development, to determine participant attitudes toward tourism, and to determine the obstacles to tourism development. The findings of this project address the complexity of operating in the tourism industry, the impacts of tourism, and the use of community based tourism models in support of sustainable tourism.
Date: May 2017
Creator: Schutz, Michael
System: The UNT Digital Library
Community College Student Retention and Completion based on Financial Expenditures and Hispanic-Serving Status (open access)

Community College Student Retention and Completion based on Financial Expenditures and Hispanic-Serving Status

Despite declining community college funding being allocated increasingly on the basis of student success, U.S. community college student retention and completion rates over the past decade have either remained steady or decreased, especially for Latino students. Using descriptive statistics and multiple regression models with secondary data procured from the Integrated Postsecondary Education Data System (IPEDS), I analyzed student success rates—full time student retention and completion rates—based on community college financial allocations and Hispanic-serving institution (HSI) status. To equitably analyze community colleges in the sample (n = 909), I separated them into four groups based on institutional size as defined by the Carnegie Classification. Descriptive results indicated that instructional divisions spent an average of 43% of the college's total allocated budget—often more than three times the allocated budget of any other division. Regression results indicated that instructional expenditures had the most consistent impact on student success regardless of college size and that scholarship expenditures and academic support expenditures generally had a negative impact on student retention and completion rates. Regarding Latino student success in particular, findings indicated that the manner in which colleges allocated their funds impacted only small and medium-sized community colleges. Of the nine different types of institutional expenditures, …
Date: May 2017
Creator: Da Silva, Jose E.
System: The UNT Digital Library
A Comparative Evaluation of Matrix Training Arrangements (open access)

A Comparative Evaluation of Matrix Training Arrangements

A common goal of instructional techniques is to teach skills effectively and efficiently. Matrix training techniques are both effective and efficient as they allow for the emergence of untrained responding to novel stimulus arrangements, a phenomenon known as recombinative generalization. However, it is unclear which type of matrix arrangement best promotes recombinative generalization. The current study compared two common matrix training approaches, an overlapping (OV) design and a non-overlapping (NOV) design, with respect to arranging relations targeted for training. We conducted a replication evaluation of a Wilshire and Toussaint study, and taught two typically-developing preschoolers compound object-action labels in Spanish and used either an OV or NOV matrix training design. Results from both studies demonstrated the participant trained with an OV design produced recombinative generalization and participants trained with a NOV design produced significantly low levels of emergence or none at all. These results suggest that an OV matrix design facilitates recombinative generalization more effectively than a NOV design. Implications for instructional arrangements are discussed.
Date: May 2017
Creator: Cliett, Terra N.
System: The UNT Digital Library
Comparing Media Usage of Binary and Non-Binary Transgender Individuals when Discovering and Describing Gender Identity (open access)

Comparing Media Usage of Binary and Non-Binary Transgender Individuals when Discovering and Describing Gender Identity

This study was conducted through in-depth interviews to examine potential differences between binary-aligned transgender individuals and non-binary individuals in regards to media usage when learning about, articulating, and explaining their gender identity. Results showed numerous differences between transgender people with binary-aligned and non-binary gender identifications in regards to social media preferences and differences in perceived media importance and effects. Additional information was found in regards to the age at which gender identity is articulated and the importance of individuality in comparison to one's gender identity.
Date: May 2017
Creator: Laljer, David "Jessie"
System: The UNT Digital Library
Composer/Performer Collaboration as Seen in the Solo Piano Part of Percy Grainger's Edition of the Edvard Grieg Piano Concerto in A Minor Opus 16 (open access)

Composer/Performer Collaboration as Seen in the Solo Piano Part of Percy Grainger's Edition of the Edvard Grieg Piano Concerto in A Minor Opus 16

The purpose of this document is threefold. First, it demonstrates what Grieg contributes to the musical text compared with the original Peters edition, particularly, those additions that refer to expression, interpretation, and style. Second, this document focuses on presenting Grainger's changes that were approved by Grieg. Third, the document evaluates Grainger's own suggestions for pedaling, hand redistribution and fingering, addition of notes, tempo markings, and other performance guidelines.
Date: May 2017
Creator: Lee, Sungyo
System: The UNT Digital Library
Consequences of Coworker Bullying: A Bystander Perspective (open access)

Consequences of Coworker Bullying: A Bystander Perspective

Previous research on workplace bullying primarily focuses on two main actors – the bully and the victim – while neglecting a third actor: the bystander of the bullying. The prevalence of workplace bullying is increasing across organizations, resulting in more employees becoming subjected to the effects of workplace bullying. Furthermore, witnessing coworker-on-coworker bullying is likely to influence the relationships that the bystander has with the two coworkers involved in the bullying episode. Two areas are proposed to investigate their effect on the coworker bystander: coworker interpersonal justice and personal identification with coworkers. Coworker interpersonal justice involves the perceived fairness between coworkers, while personal identification refers to how these bystanders identify with the specific actors of the bullying event. In addition to work-related outcomes, bystanders are affected at a personal level. That is, being exposed to bullying situations causes these bystanders to alter their anxiety levels and their core affect, with core affect being a precursor to moods and emotions. In addition to the aforementioned outcomes of witnessing a coworker bullying incident, there are also contextual aspects which may influence these relationships. Personal-level factors, such as a bystander's empathy and sense of coherence (i.e., coping mechanisms), may influence the effect of …
Date: May 2017
Creator: Medina, Michele N.
System: The UNT Digital Library
Construct Validation and Measurement Invariance of the Athletic Coping Skills Inventory for Educational Settings (open access)

Construct Validation and Measurement Invariance of the Athletic Coping Skills Inventory for Educational Settings

The present study examined the factor structure and measurement invariance of the revised version of the Athletic Coping Skills Inventory (ACSI-28), following adjustment of the wording of items such that they were appropriate to assess Coping Skills in an educational setting. A sample of middle school students (n = 1,037) completed the revised inventory. An initial confirmatory factor analysis led to the hypothesis of a better fitting model with two items removed. Reliability of the subscales and the instrument as a whole was acceptable. Items were examined for sex invariance with differential item functioning (DIF) using item response theory, and five items were flagged for significant sex non-invariance. Following removal of these items, comparison of the mean differences between male and female coping scores revealed that there was no significant difference between the two groups. Further examination of the generalizability of the coping construct and the potential transfer of psychosocial skills between athletic and academic settings are warranted.
Date: May 2017
Creator: Sanguras, Laila Y., 1977-
System: The UNT Digital Library
A Constructional Approach to Establishing and Maintaining Calm Canine Behavior (open access)

A Constructional Approach to Establishing and Maintaining Calm Canine Behavior

Very few behavior-change programs with canines produce effects that persist beyond the training condition. The present study is an experimental demonstration of a constructional program that established calm patterns of behavior as alternatives to hyperactive ones. Three dogs that exhibited hyperactive patterns were chosen as subjects. Seven conditions common to canine-caretaker relationships were used to determine which factors resulted in the hyperactive patterns. Then, sitting and lying down were taught as beginning points using touch as a reinforcer. The final behavior, maintained by naturally occurring reinforcers, was established errorlessly. The study used a control-analysis strategy of behavior change with a changing-criterion design. The intervention resulted in an immediate reduction in hyperactivity and an increase in sitting and lying down for all dogs.
Date: May 2017
Creator: Owens, Chase Jonathan
System: The UNT Digital Library
A Content Analysis of Medical School Problem-Based Learning Cases (open access)

A Content Analysis of Medical School Problem-Based Learning Cases

Problem-based learning (PBL) was developed for use in medical education to incorporate more active, learner-centered instruction. Central to problem-based learning is the problem, which in medical education is usually case a case presentation, revealed in stages to allow learners to form and research learning objectives. The purpose of this study was to identify themes present across the PBL cases, including the patient-centeredness of the cases. Content analysis was used to examine 62 PBL cases that comprised the first and second years' core curriculum at a public medical school. The cases included a patient population similar to the local population, but care was more hospital-centric than would be expected from the actual patterns of medical utilization in the United States. Analyzing along two axes of patient-centeredness, the PBL cases demonstrated a good understanding of the patient (knowing the patient), but other qualities such as shared decision making was not as exemplified. Medical educators can use the results to understand elements that contribute to patient-centeredness and apply the analysis framework to evaluate future cases.
Date: May 2017
Creator: Kinkade, Scott
System: The UNT Digital Library
Content and Temporal Analysis of Communications to Predict Task Cohesion in Software Development Global Teams (open access)

Content and Temporal Analysis of Communications to Predict Task Cohesion in Software Development Global Teams

Virtual teams in industry are increasingly being used to develop software, create products, and accomplish tasks. However, analyzing those collaborations under same-time/different-place conditions is well-known to be difficult. In order to overcome some of these challenges, this research was concerned with the study of collaboration-based, content-based and temporal measures and their ability to predict cohesion within global software development projects. Messages were collected from three software development projects that involved students from two different countries. The similarities and quantities of these interactions were computed and analyzed at individual and group levels. Results of interaction-based metrics showed that the collaboration variables most related to Task Cohesion were Linguistic Style Matching and Information Exchange. The study also found that Information Exchange rate and Reply rate have a significant and positive correlation to Task Cohesion, a factor used to describe participants' engagement in the global software development process. This relation was also found at the Group level. All these results suggest that metrics based on rate can be very useful for predicting cohesion in virtual groups. Similarly, content features based on communication categories were used to improve the identification of Task Cohesion levels. This model showed mixed results, since only Work similarity and …
Date: May 2017
Creator: Castro Hernandez, Alberto
System: The UNT Digital Library
A Content Originality Analysis of HRD Focused Dissertations and Published Academic Articles using TurnItIn Plagiarism Detection Software (open access)

A Content Originality Analysis of HRD Focused Dissertations and Published Academic Articles using TurnItIn Plagiarism Detection Software

This empirical exploratory study quantitatively analyzed content similarity indices (potential plagiarism) from a corpus consisting of 360 dissertations and 360 published articles. The population was defined using the filtering search criteria human resource development, training and development, organizational development, career development, or HRD. This study described in detail the process of collecting content similarity analysis (CSA) metadata using Turnitin software (www.turnitin.com). This researcher conducted robust descriptive statistics, a Wilcoxon signed-rank statistic between the similarity indices before and after false positives were excluded, and a multinomial logistic regression analysis to predict levels of plagiarism for the dissertations and the published articles. The corpus of dissertations had an adjusted rate of document similarity (potential plagiarism) of M = 9%, (SD = 6%) with 88.1% of the dissertations in the low level of plagiarism, 9.7% in the high and 2.2% in the excessive group. The corpus of published articles had an adjusted rate of document similarity (potential plagiarism) of M = 11%, (SD = 10%) with 79.2% of the published articles in the low level of plagiarism, 12.8% in the high and 8.1% in the excessive group. Most of the difference between the dissertations and published articles were attributed to plagiarism-of-self issues which …
Date: May 2017
Creator: Mayes, Robin James
System: The UNT Digital Library
Creating Discussion: An Auteur Analysis of Films Directed by Adrian Lyne (open access)

Creating Discussion: An Auteur Analysis of Films Directed by Adrian Lyne

This thesis examines the various "signature" threads that are present within the "oeuvre" of the Hollywood filmmaker Adrian Lyne. The goal of this thesis is to showcase both how and why Lyne can be thought of as an auteur and to open up his films to new and previously unexplored meanings. Lyne's eight feature films are analyzed in-depth individually and in comparison to one another from a variety of theoretical frameworks and points of focus in each of the body chapters.
Date: May 2017
Creator: Oliver, Stephanie
System: The UNT Digital Library
Culturally Competent Evaluations (open access)

Culturally Competent Evaluations

Significant growth in the number of English language learners (ELLs) in U.S. schools is anticipated to continue, demanding that educators and evaluators have the skills necessary to distinguishing language difference from disability and provide appropriate services to these students. However, little research exists examining the role of evaluator's cultural competence in evaluating ELLs for special education; furthermore, what does exist shows that many evaluators report low levels of self-efficacy as it relates to assessing ELLs. The first chapter of the dissertation reports on a review of 21 articles conducted to address best practices for evaluating culturally and linguistically diverse (CLD) learners, evaluator self-efficacy, and recommendations for conducting culturally competent assessments and evaluations. The majority of the articles identified in this review focused on recommendations for best practices for conducting evaluations rather than reporting empirical findings related to the topic. Only one study was identified that focused on appropriate training needed by evaluation staff to effectively discriminate between language difference and a disability. Based on the findings of this review, additional research, using a rigorous methodology is needed. Addressing that need, the second chapter reports the results of a study conducted to examine the effectiveness of Project PEAC3E (Preparing Evaluators to …
Date: May 2017
Creator: Chen, Cristina Rodríguez
System: The UNT Digital Library
Design Considerations and Implementation of Portable Mass Spectrometers for Environmental Applications (open access)

Design Considerations and Implementation of Portable Mass Spectrometers for Environmental Applications

Portable mass spectrometers provide a unique opportunity to obtain in situ measurements. This minimizes need for sample collection or in laboratory analysis. Membrane Inlet Mass Spectrometry (MIMS) utilizing a semi permeable membrane for selective rapid introduction for analysis. Polydimethylsiloxane membranes have been proven to be robust in selecting for aromatic chemistries. Advances in front end design have allowed for increased sensitivity, rapid sample analysis, and on line measurements. Applications of the membrane inlet technique have been applied to environmental detection of clandestine drug chemistries and pollutants. Emplacement of a mass spectrometer unit in a vehicle has allowed for large areas to be mapped, obtaining a rapid snapshot of the various concentrations and types of environmental pollutants present. Further refinements and miniaturization have allowed for a backpackable system for analysis in remote harsh environments. Inclusion of atmospheric dispersion modeling has yielded an analytical method of approximating upwind source locations, which has law enforcement, military, and environmental applications. The atmospheric dispersion theories have further been applied to an earth based separation, whereby chemical properties are used to approximate atmospheric mobility, and chemistries are further identified has a portable mass spectrometer is traversed closer to a point source.
Date: May 2017
Creator: Mach, Phillip M.
System: The UNT Digital Library
Design, Modeling, and Experiment of a Piezoelectric Pressure Sensor based on a Thickness-Shear Mode Crystal Resonator (open access)

Design, Modeling, and Experiment of a Piezoelectric Pressure Sensor based on a Thickness-Shear Mode Crystal Resonator

This thesis presents the design, modeling, and experiment of a novel pressure sensor using a dual-mode AT-cut quartz crystal resonator with beat frequency analysis based temperature compensation technique. The proposed sensor can measure pressure and temperature simultaneously by a single AT-cut quartz resonator. Apart from AT-cut quartz crystal, a newly developed Langasite (LGS) crystal resonator is also considered in the proposed pressure sensor design, since LGS can operate in a higher temperature environment than AT-cut quartz crystal. The pressure sensor is designed using CAD (computer aided design) software and CAE software - COMSOL Multiphysics. Finite element analysis (FEA) of the pressure sensor is performed to analyze the stress- strain of the sensor's mechanical structure. A 3D printing prototype of the sensor is fabricated and the proposed sensing principle is verified using a force-frequency analysis apparatus. Next to the 3D printing model verification, the pressure sensor with stainless steel housing has been fabricated with inbuilt crystal oscillator circuit. The oscillator circuit is used to excite the piezo crystal resonator at its fundamental vibrational mode and give the frequency as an output signal. Based on the FEA and experimental results, it has been concluded that the maximum pressure that the sensor can …
Date: May 2017
Creator: Pham, Thanh Tuong
System: The UNT Digital Library
Design of a Lower Extremity Exoskeleton to Increase Knee ROM during Valgus Bracing for Osteoarthritic Gait (open access)

Design of a Lower Extremity Exoskeleton to Increase Knee ROM during Valgus Bracing for Osteoarthritic Gait

Knee osteoarthritis (KOA) is the primary cause of chronic immobility in populations over the age of 65. It is a joint degenerative disease in which the articular cartilage in the knee joint wears down over time, leading to symptoms of pain, instability, joint stiffness, and misalignment of the lower extremities. Without intervention, these symptoms gradually worsen over time, decreasing the overall knee range of motion (ROM) and ability to walk. Current clinical interventions include offloading braces, which mechanically realign the lower extremities to alleviate the pain experienced in the medial compartment of the knee joint. Though these braces have proven effective in pain management, studies have shown a significant decrease in knee ROM while using the brace. Concurrently, development of active exoskeletons for rehabilitative gait has increased within recent years in efforts to provide patients with a more effective intervention for dealing with KOA. Though some developed exoskeletons are promising in their efficacy of fostering gait therapy, these devices are heavy, tethered, difficult to control, unavailable to patients, or costly due to the number of complicated components used to manufacture the device. However, the idea that an active component can improve gait therapy for patients motivates this study. This study …
Date: May 2017
Creator: Cao, Jennifer M.
System: The UNT Digital Library
Design of a Machine Condition Monitoring System with Bluetooth Low Energy (open access)

Design of a Machine Condition Monitoring System with Bluetooth Low Energy

This thesis discusses the design considerations for a machine conditioning sensor utilizing Bluetooth low energy (BLE).
Date: May 2017
Creator: Tompkins, Nicholas
System: The UNT Digital Library
Design Principle on Carbon Nanomaterials Electrocatalysts for Energy Storage and Conversion (open access)

Design Principle on Carbon Nanomaterials Electrocatalysts for Energy Storage and Conversion

We are facing an energy crisis because of the limitation of the fossil fuel and the pollution caused by burning it. Clean energy technologies, such as fuel cells and metal-air batteries, are studied extensively because of this high efficiency and less pollution. Oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) are essential in the process of energy storage and conversion, and noble metals (e.g. Pt) are needed to catalyze the critical chemical reactions in these devices. Functionalized carbon nanomaterials such as heteroatom-doped and molecule-adsorbed graphene can be used as metal-free catalysts to replace the expensive and scarce platinum-based catalysts for the energy storage and conversion. Traditionally, experimental studies on the catalytic performance of carbon nanomaterials have been conducted extensively, however, there is a lack of computational studies to guide the experiments for rapid search for the best catalysts. In addition, theoretical mechanism and the rational design principle towards ORR and OER also need to be fully understood. In this dissertation, density functional theory calculations are performed to calculate the thermodynamic and electrochemical properties of heteroatom-doped graphene and molecule-adsorbed graphene for ORR and OER. Gibb's free energy, overpotential, charge transfer and edge effect are evaluated. The charge transfer analysis show …
Date: May 2017
Creator: Zhao, Zhenghang
System: The UNT Digital Library
Determinants of Corporate Governance Choices: Evidence from Listed Foreign Firms on U.S. Stock Exchanges (open access)

Determinants of Corporate Governance Choices: Evidence from Listed Foreign Firms on U.S. Stock Exchanges

This study analyzes corporate governance practices of foreign (non-U.S.) issuers listed on the New York Stock Exchange (NYSE) and Nasdaq. Specifically, I examine the extent to which these foreign issuers voluntarily comply with U.S. stock exchange corporate governance requirements applicable to domestic issuers. My sample consists of 201 foreign companies primarily domiciled in Brazil, China, Israel, and the United Kingdom. I find that 151 (75 per cent) of the sample firms do not elect to comply with any of the U.S. corporate governance requirements. Logistic regression analysis generally supports the hypotheses that conformance with U.S. GAAP and percentage of managerial ownership are positively associated, and that percentage ownership by major shareholders is negatively associated with foreign firms electing to comply with U.S. corporate governance rules. This evidence is relevant for regulators and investors.
Date: May 2017
Creator: Attachot, Weerapat
System: The UNT Digital Library
Determining Whether and When People Participate in the Events They Tweet About (open access)

Determining Whether and When People Participate in the Events They Tweet About

This work describes an approach to determine whether people participate in the events they tweet about. Specifically, we determine whether people are participants in events with respect to the tweet timestamp. We target all events expressed by verbs in tweets, including past, present and events that may occur in future. We define event participant as people directly involved in an event regardless of whether they are the agent, recipient or play another role. We present an annotation effort, guidelines and quality analysis with 1,096 event mentions. We discuss the label distributions and event behavior in the annotated corpus. We also explain several features used and a standard supervised machine learning approach to automatically determine if and when the author is a participant of the event in the tweet. We discuss trends in the results obtained and devise important conclusions.
Date: May 2017
Creator: Sanagavarapu, Krishna Chaitanya
System: The UNT Digital Library
Development of an Outcome Measure for Use in Psychology Training Clinics (open access)

Development of an Outcome Measure for Use in Psychology Training Clinics

The ability to monitor client change in psychotherapy over time is vital to quality assurance in service delivery as well as the continuing improvement of psychotherapy research. Unfortunately, there is not currently a comprehensive, affordable, and easily utilized outcome measure for psychotherapy specifically normed and standardized for use in psychology training clinics. The current study took the first steps in creating such an outcome measure. Following development of an item bank, factor analysis and item-response theory analyses were applied to data gathered from a stratified sample of university (n = 101) and community (n = 261) participants. The factor structure did not support a phase model conceptualization, but did reveal a structure consistent with the theoretical framework of the research domain criteria (RDoC). Suggestions for next steps in the measure development process are provided and implications discussed.
Date: May 2017
Creator: Davis, Elizabeth C.
System: The UNT Digital Library
Development of von Willebrand Factor Zebrafish Mutant Using CRISPR/Cas9 Mediated Genome Editing (open access)

Development of von Willebrand Factor Zebrafish Mutant Using CRISPR/Cas9 Mediated Genome Editing

von Willebrand factor (VWF) protein acts in the intrinsic coagulation pathway by stabilizing FVIII from proteolytic clearance and at the site of injury, by promoting the adhesion and aggregation of platelets to the exposed subendothelial wall. von Willebrand disease (VWD) results from quantitative and qualitative deficiencies in VWF protein. The variability expressivity in phenotype presentations is in partly caused by the action of modifier genes. Zebrafish has been used as hemostasis animal model. However, it has not been used to evaluate VWD. Here, we report the development of a heterozygote VWF mutant zebrafish using the genome editing CRISPR/Cas9 system to screen for modifier genes involved in VWD. We designed CRISPR oligonucleotides and inserted them into pT7-gRNa plasmid. We then prepared VWF gRNA along with the endonuclease Cas9 RNA from Cas9 plasmid. We injected these two RNAs into 1-4 cell-stage zebrafish embryos and induced a mutation in VWF exon 29 of the zebrafish with a mutagenesis rate of 16.6% (3/18 adult fish). Also, we observed a germline transmission with an efficiency rate of 5.5% (1/18 adult fish). We obtained a deletion in exon 29 which should result in truncated VWF protein.
Date: May 2017
Creator: Toffessi Tcheuyap, Vanina
System: The UNT Digital Library
Disease Tissue Imaging and Single Cell Analysis with Mass Spectrometry (open access)

Disease Tissue Imaging and Single Cell Analysis with Mass Spectrometry

Cells have been found to have an inherent heterogeneity that has led to an increase in the development of single-cell analysis methods to characterize the extent of heterogeneity that can be found in seemingly identical cells. With an understanding of normal cellular variability, the identification of disease induced cellular changes, known as biomarkers, may become more apparent and readily detectable. Biomarker discovery in single-cells is challenging and needs to focus on molecules that are abundant in cells. Lipids are widely abundant in cells and play active roles in cellular signaling, energy metabolism, and are the main component of cellular membranes. The regulation of lipid metabolism is often disrupted or lost during disease progression, especially in cancer, making them ideal candidates as biomarkers. Challenges exist in the analysis of lipids beyond those of single-cell analysis. Lipid extraction solvents must be compatible with the lipid or lipids of interest. Many lipids are isobaric making mass spectrometry analysis difficult without separations. Single-cell extractions using nanomanipulation coupled to mass spectrometry has shown to be an excellent method for lipid analysis of tissues and cell cultures. Extraction solvents are tunable for specific lipid classes, nanomanipulation prevents damage to neighboring cells, and lipid separations are possible …
Date: May 2017
Creator: Hamilton, Jason S.
System: The UNT Digital Library