Introduction/purpose: Recovery from muscle damage involves a type of programmed cell death known as apoptosis. Damage Associated Molecular Patterns (DAMPs) are released after muscle damage and may cause premature apoptosis in monocytes infiltrating the damaged site. This may alter the time course of events towards recovery. Therefore, the purpose of this study was to investigate if downhill running causes a change in the susceptibility of monocytes to apoptosis. Methods: Participants (5 male, 6 female) completed a downhill running protocol consisting of 6-5 minute bouts at a speed of 6-9mph on a -15% grade treadmill. Venous blood samples were collected immediately pre-exercise (PRE), in addition to 4 -h, 24 -h and 48 -h post-exercise. Creatine kinase (CK) was measured to give an indication of muscle damage. Monocytes were analyzed by flow cytometry for expression of multicaspase and annexin v reagent was used to detect changes in the plasma membrane. A MILLIPLEX MAP human early apoptosis magnetic bead 7-plex kit (EMD Millipore, Billerica, MA) was used to assess the relative concentration of phosphorylated protein kinase B (Akt), Bcl-2 associated death promoter (BAD), B cell lymphoma-2 (Bcl-2), active caspase-8, active caspase-9, c jun N terminal kinase (JNK) and tumor protein p53 by Luminex …

This study investigated the relationship between belief in coaching abilities (coaching efficacy beliefs, CEB), transformational leadership behaviors (TLB), and motivational climate development of current strength and conditioning coaches working with high school level athletes. The measures used were the coaching efficacy scale for high school teams (CES II-HST, Myers et al.,2000), the differentiated transformational leadership inventory (DTLI, Callow et al., 2009), and the patterns of adaptive learning scales (PALS, Midgley et al., 2000). It was hypothesized that CEB and TLB would influence motivational climate development, while coaches' background characteristics would correlate with CEB, TLB, and motivational climate development. The 60 coaches who participated reported an average of thirteen (SD=8) years of experience and 51 were Certified Strength and Conditioning Specialists. Coaches reported high efficacy, frequent use of TLB, and development of a moderately high task- and somewhat ego-involving motivational climate. Correlations between demographic variables and CEB, TLB, and motivational climate development revealed three significant relationships: years of experience with CEB, and professional development activities and athlete to coach ratio with ego-involving climate development. CEB and TLB had a strong positive correlation. Two regression analyses were conducted to determine if the outcomes of the CEB and TLB measures predicted motivational climate …