Degree Level

48 Matching Results

Results open in a new window/tab.

On the Fence (open access)

On the Fence

Living the vast majority of my life in an area that celebrates diversity but thrives because of illegal cross-border activities (undocumented workers, drug imports) at times the distance between the United States and Mexico is in fact as thin as the width of a fence. Though it is typical for a filmmaker to hope to present a unique take on a subject, given how I have seen the topics of immigration and the perspective of the purpose of homeland security portray, I am confident that there is an opportunity to show these issues in a more personal, less aggressive light with the use of first person accounts instead of a dependence on the most violent aspects of these topics. The main subject will give character to this agency by blurring the lines of his life as an agent and as a citizen.
Date: December 2014
Creator: Medrano, Estevan
System: The UNT Digital Library
Development and Test of High-Temperature Piezoelectric Wafer Active Sensors for Structural Health Monitoring (open access)

Development and Test of High-Temperature Piezoelectric Wafer Active Sensors for Structural Health Monitoring

High-temperature piezoelectric wafer active sensors (HT-PWAS) have been developed for structure health monitoring at hazard environments for decades. Different candidates have previously been tested under 270 °C and a new piezoelectric material langasite (LGS) was chosen here for a pilot study up to 700 °C. A preliminary study was performed to develop a high temperature sensor that utilizes langasite material. The Electromechanical impedance (E/M) method was chosen to detect the piezoelectric property. Experiments that verify the basic piezoelectric property of LGS at high temperature environments were carried out. Further validations were conducted by testing structures with attached LGS sensors at elevated temperature. Additionally, a detection system simulating the working process of LGS monitoring system was developed with PZT material at room temperature. This thesis, for the first time, (to the best of author’s knowledge) presents that langasite is ideal for making piezoelectric wafer active sensors for high temperature structure health monitoring applications.
Date: December 2014
Creator: Bao, Yuanye
System: The UNT Digital Library
Assessing Workplace Design: Applying Anthropology to Assess an Architecture Firm’s Own Headquarters Design (open access)

Assessing Workplace Design: Applying Anthropology to Assess an Architecture Firm’s Own Headquarters Design

Corporations, design firms, technology, and furniture companies are rethinking the concept of the ‘workplace’ environment and built ‘office’ in an effort to respond to changing characteristics of the workplace. The following report presents a case study, post-occupancy assessment of an architecture firm’s relocation of their corporate headquarters in Dallas, TX. This ethnographic research transpired from September 2013 to February 2014 and included participant observation, employee interviews, and an office-wide employee survey. Applying a user-centered approach, this study sought to identify and understand: 1) the most and least effective design elements, 2) unanticipated user-generated (“un-designed”) elements, 3) how the workplace operates as an environment and system of design elements, and 4) opportunities for continued improvement of their work environment. This study found that HKS ODC successfully increased access to collaborative spaces by increasing the size (i.e. number of square feet, number of rooms), variety of styles (i.e. enclosed rooms, open work surfaces), and distribution of spaces throughout the office environment. An increase in reported public transit commuting from 6.5% at their previous location to 24% at HKS ODC compares to almost five times the national public transit average (5%) and fifteen times the rate of Texas workers (1.6%) and Dallas-Fort Worth-Arlington, …
Date: December 2014
Creator: Ramer, S. Angela
System: The UNT Digital Library
Surface Topography and Aesthetics of Recycled Cross-Linked Polyethylene Wire and Cable Coatings (open access)

Surface Topography and Aesthetics of Recycled Cross-Linked Polyethylene Wire and Cable Coatings

Our research focuses on re-using a waste a material, cross-linked polyethylene abbreviated XLPE, which is a widely used coating for wires. XLPE is strong and has excellent thermal properties due to its chemical structure - what leads to the significance of recycling this valuable polymer. Properties of XLPE include good resistance to heat, resistance to chemical corrosion, and high impact strength. A wire is usually composed of a metal core conductor and polymeric coating layers. One creates a new coating, including little pieces of recycled XLPE in the lower layer adjacent to the wire, and virgin XLPE only in the upper layer. Industries are often wasting materials which might be useful. Mostly, some returned or excess products could be recycled to create a new type of product or enable the original use. This method helps cleaning the waste, lowers the costs, and enhances the income of the manufacturing company. With the changing of the thickness of the outer layer, the roughness changes significantly. Moreover, different processing methods result in surfaces that look differently.
Date: December 2014
Creator: Xie, Wa
System: The UNT Digital Library
Dismantling the Psychiatric Ghetto: Evaluating a Blended-Clinic Approach to Supportive Housing in Houston, Texas (open access)

Dismantling the Psychiatric Ghetto: Evaluating a Blended-Clinic Approach to Supportive Housing in Houston, Texas

Locational decisions based on stigma and low funding have handicapped the efficiency of community based mental healthcare in the United States since 1963. However, the pattern of services in the 21st century American South remains largely unknown. This thesis addresses this gap in knowledge by using a mixed methodology including location allocation, descriptive statistics, and qualitative site visits to explore the geography of community clinics offering both physical and mental health services. The City of Houston has proposed using these facilities to anchor new supportive housing, but introducing more fixed costs to a mismatched system could create more problems than solutions. The findings of this study suggest the presence of an unnecessary concentration of services in the central city and a spatial mismatch between accessible clinics and the poor, sick people in need. Furthermore, this research reveals a new suburban pattern of vulnerability, calling into question long-held assumptions about the vulnerability of the inner city. Building supportive housing around existing community clinics, especially in the central city, may further concentrate vulnerable people thereby contributing to intensifying patterns of service-seeking drift and the continued traumatization of mentally ill homeless persons in Houston.
Date: December 2014
Creator: Lester, Katherine Ann
System: The UNT Digital Library
Considering Canine Companionship: An Examination of Dog Owner Travel Desires Using the Model of Goal-Directed Behavior (open access)

Considering Canine Companionship: An Examination of Dog Owner Travel Desires Using the Model of Goal-Directed Behavior

The purpose of this study is to investigate how internal and external sources influence dog owners’ desire and intent to travel with their dogs, using the model of goal-directed behavior (MGB). Specifically, this study investigates 1) the demographic profile of participating dog owners, 2) the relationship between dog owners’ Anticipated Emotions (AE) and their desire to travel with their dogs, 3) dog owners’ Attitudes toward the act (Aact) of traveling with their dogs and its relationship with their desire to travel with dogs, 4) the relationship between Subjective Norms (SN) and dog owners desire to travel with dogs, 5) owners’ Perceived Behavioral Control (PBC) over their dog-accompanied travel situation, 6) the relationship between desire for dog-accompanied travel and Behavioral Intent (BI), and 7) the relationship between Past Behavior (PB) and the desire and BI regarding future travel with dogs.
Date: December 2014
Creator: Krier, J. Leia
System: The UNT Digital Library
Why Be Friends? Amicus Curiae Briefs in State Courts of Last Resort (open access)

Why Be Friends? Amicus Curiae Briefs in State Courts of Last Resort

While there has been a substantial body of research on interest group activity in U.S. federal courts, there has been comparatively little analysis of interest group engagement with state courts. Given that state courts adjudicate the vast majority of cases in the American legal system and very few cases are appealed to the Supreme Court, understanding why organized interests participate in these courts is of great importance. The present study analyzes interest group involvement as amicus curiae in all state courts of last resort from 1995-1999 to examine what factors motivate organized interests to turn to the courts. The results indicate that interest groups are primarily motivated by their policy goals in deciding which cases to file amicus briefs in, but that they are limited in their ability to file by institutional constraints unique to state courts of last resort. This research provides insight into interest group behavior, state courts and the role organized interests play in influencing legal outcomes in the American states.
Date: December 2014
Creator: Perkins, Jared D.
System: The UNT Digital Library
Resource Intensification of Small Game Use at Goodman Point, Southwestern Colorado (open access)

Resource Intensification of Small Game Use at Goodman Point, Southwestern Colorado

This analysis of faunal remains from eleven archaeological sites in the northern San Juan region, extensively occupied by the Ancestral Pueblo people until they leave the region by AD 1300, explores the effects of resource intensification of small wild and domestic resources leading up to this regional depopulation. By examining multiple lines of evidence, in addition to faunal abundance, causal factors are identified to address changes in abundances through time. In particular, age- and sex-based mortality are examined for lagomorphs (jackrabbits and cottontails) and domesticated turkey, respectively, to test hypotheses generated using the prey and patch choice models. Analyses of these resources follow a systematic paleontology which provides explicit identifications made of five sites from a large study area, Goodman Point Pueblo Unit. These data are integrated with those from large village sites from the encompassing central Mesa Verde region. The results of both analyses help clarify why the Ancestral Pueblo people left southwestern Colorado. During the final twenty-year occupation period, the results of this study support a shift from reliance on turkey husbandry to intense exploitation of locally available garden resources (i.e. cottontails).
Date: December 2014
Creator: Ellyson, Laura Jean
System: The UNT Digital Library
Violetting Through August’s End (Or the Sunset in Water, the Carillon-chime in Square): an Original Chamber Opera and a Critical Essay on the Trajectory of American Minimalist Opera (open access)

Violetting Through August’s End (Or the Sunset in Water, the Carillon-chime in Square): an Original Chamber Opera and a Critical Essay on the Trajectory of American Minimalist Opera

When the dust settles, John Adams’s Nixon in China and Philip Glass’s Einstein on the Beach may stand as the most important operas of the latter twentieth century. The critical essay portion of this thesis examines the trajectory of minimalist opera, from its beginnings with Glass’s Einstein on the Beach through the more romantic operas of John Adams, Steve Reich’s multimedia opera The Cave, David Lang’s musical-influenced The Difficulty of Crossing a Field, and finally the post-minimalist operas currently being staged by young composer Nico Muhly. It examines the differences between the more abstract trajectory established by the early Glass operas and the plot driven trajectory established by operas more commonly associated with John Adams, most significantly Nixon in China. Additionally, the aforementioned pieces are compared and contrasted with the author’s newly composed chamber opera Violetting through August’s End (or the sunset in water, the carillon-chime in square).
Date: December 2014
Creator: Doyle, James Joseph
System: The UNT Digital Library
Kinetic Investigation of Atomic Hydrogen with Sulfur-Containing Species (open access)

Kinetic Investigation of Atomic Hydrogen with Sulfur-Containing Species

The reactions of atomic hydrogen with methanethiol and that of atomic hydrogen with carbon disulfide were studied experimentally using flash-photolysis resonance-fluorescence techniques. Rate constants were determined over a range of temperatures and pressures, and through analysis and comparison to theoretical work details of the reactions were ascertained.
Date: December 2014
Creator: Kerr, Katherine Elaine
System: The UNT Digital Library
Effects of Non-homogeneous Population Distribution on Smoothed Maps Produced Using Kernel Density Estimation Methods (open access)

Effects of Non-homogeneous Population Distribution on Smoothed Maps Produced Using Kernel Density Estimation Methods

Understanding spatial perspectives on the spread and incidence of a disease is invaluable for public health planning and intervention. Choropleth maps are commonly used to provide an abstraction of disease risk across geographic space. These maps are derived from aggregated population counts that are known to be affected by the small numbers problem. Kernel density estimation methods account for this problem by producing risk estimates that are based on aggregations of approximately equal population sizes. However, the process of aggregation often combines data from areas with non-uniform spatial and population characteristics. This thesis presents a new method to aggregate space in ways that are sensitive to their underlying risk factors. Such maps will enable better public health practice and intervention by enhancing our ability to understand the spatial processes that result in disparate health outcomes.
Date: December 2014
Creator: Jones, Jesse Jack
System: The UNT Digital Library
Still on the Sidelines: the Female Experience in Sports Media (open access)

Still on the Sidelines: the Female Experience in Sports Media

This qualitative study aims to analyze the lived reality of women working in sports media today. Through systematic analysis of 12 in-depth interviews, the findings of this study suggest that the adoption of technological advancements in news media and all associated outlets have created a leveling effect for women due to the demand for highly skilled individuals who can handle the digital demand of modern news production. This study suggests that longtime gender disparities in sports media are experiencing a bit of a reprieve due to the massive digital audience and the need for professionals who can deliver information quickly and efficiently and with accuracy. However, the persistent symbolic annihilation of women as well as hegemonic hiring practices that emphasize aesthetic appeal have created a difficult path for women to move off the sidelines and into roles with more creative and analytical breadth, even with a rapidly increasing demand for jobs in the media industry.
Date: December 2014
Creator: Blankenship, Sara K.
System: The UNT Digital Library
The Academic Steroid: Nonmedical Use of Prescription Stimulants at a North Texas University (open access)

The Academic Steroid: Nonmedical Use of Prescription Stimulants at a North Texas University

The goal of this study was to determine the extent, motivations, and justifications of nonmedical prescription stimulant use among the population at a large public university in the North Texas region. Participants consisted of 526 undergraduate students enrolled at the studied university during the spring and summer 2014 semesters. The findings of the study suggest that the nonmedical use by students was higher than the findings in much of the current literature, but was within the parameters established in the literature. The primary motivation for nonmedical use was academic in nature and was justified by moderation of nonmedical use to strategic academic times.
Date: December 2014
Creator: Pennington, Cody W.
System: The UNT Digital Library
The Behavioral Economics of Effort (open access)

The Behavioral Economics of Effort

Although response effort is considered a dimension of the cost to obtain reinforcement, little research has examined the economic impact of effort on demand for food. The goal of the present study was to explore the relationship between effort and demand. Three Sprague Dawley rats were trained to press a force transducer under a series of fixed-ratio schedules (1, 10, 18, 32, 56, 100, 180, 320, and 560) under different force requirements (5.6 g and 56 g). Thus, nominal unit price (responses / food) remained constant while minimal response force requirements varied. Using a force transducer allowed the measurement of responses failing to meet the minimal force requirement (i.e. “subcriterion responses”), an advantage over prior approaches using weighted levers to manipulate effort. Consistent with prior research, increasing the unit price decreased food consumption, and raising minimum force requirements further reduced demand for food. Additionally, increasing the force requirement produced subcriterion responses. Analysis indicated that subcriterion responses did not create incidental changes in unit price. Obtained force data revealed that including obtained forces in unit price calculations provided better predictions of consumption when compared to using criterion force requirements.
Date: December 2014
Creator: Nord, Christina M.
System: The UNT Digital Library
Evaluating the Feasibility of Accelerometers in Hand Gestures Recognition (open access)

Evaluating the Feasibility of Accelerometers in Hand Gestures Recognition

Gesture recognition plays an important role in human computer Interaction for intelligent computing. Major applications like Gaming, Robotics and Automated Homes uses gesture recognition techniques which diminishes the usage of mechanical devices. The main goal of my thesis is to interpret SWAT team gestures using different types of sensors. Accelerometer and flex sensors were explored extensively to build a prototype for soldiers to communicate in the absence of line of sight. Arm movements were recognized by flex sensors and motion gestures by Accelerometers. Accelerometers are used to measure acceleration in respect to movement of the sensor in 3D. Flex sensors changes its resistance based on the amount of bend in the sensor. SVM is the classification algorithm used for classification of the samples. LIBSVM (Library for Support Vector Machines) is integrated software for support vector classification, regression and distribution estimation which supports multi class classification. Sensors data is connected to the WI micro dig to digitize the signal and to transmit it wirelessly to the computing device. Feature extraction and Signal windowing were the two major factors which contribute for the accuracy of the system. Mean Average value and Standard Deviation are the two features considered for accelerometer sensor data …
Date: December 2014
Creator: Karlaputi, Sarada
System: The UNT Digital Library
Diabetes Status of Mexican Americans: Impact of Country of Birth (open access)

Diabetes Status of Mexican Americans: Impact of Country of Birth

In order to better tailor treatment to specific populations, factors which contribute to health disparities among different racial/ethnic groups must be examined. Among Mexican American individuals, the high rate of diabetes represents a significant contributor to overall health. The present study focuses on factors affecting diabetes status among Mexican Americans born in either Mexico or the United States using the 2007 – 2008 NHANES data set. Comparisons were made between diabetes status based on self-report and clinical classification using HbA1c. Results indicated that within the diabetic subsample, Mexican Americans born in Mexico were twice as likely to be incorrectly classified as non-diabetic, when they actually were diabetic, when using a self-report method. In contrast, nativity did not result in differences in diabetes incidence using the HbA1c clinical cut-score diagnostic classification. Age, BMI, gender, nativity, and health insurance coverage were found to have varying relationships to diabetes prevalence and HbA1c levels, but time in the U.S. for Mexico-born individuals was not found to uniquely predict diabetes incidence. Analyses also demonstrated that Mexico-born males, as compared to the other groups, had significantly higher HbA1c levels. Further research is necessary to better understand the relationships among these factors. However, findings do demonstrate a …
Date: December 2014
Creator: Douglas, Megan E.
System: The UNT Digital Library
Measuring Biomarkers From Dried Blood Spots Utilizing Bead-based Multiplex Technology (open access)

Measuring Biomarkers From Dried Blood Spots Utilizing Bead-based Multiplex Technology

Dried blood spots is an alternative method to collect blood samples from research subjects. However, little is known about how hemoglobin and hematocrit affect bead-based multiplex assay performance. The purpose of this study was to determine how bead-based multiplex assays perform when analyzing dried blood spot samples. A series of four experiments outline the study each with a specific purpose. A total of 167 subject samples were collected and 92 different biomarkers were measured. Median fluorescence intensity results show a positive correlation between filtered and non-filtered samples. Utilizing a smaller quantity of sample results in a positive correlation to a larger sample. Removal of hemoglobin from the dried blood spot sample does not increase detection or concentration of biomarkers. Of the 92 different biomarkers measured 56 were detectable in 100-75% of the attempted samples. We conclude that blood biomarkers can be detected using bead-based multiplex assays. In addition, it is possible to utilize a smaller quantity of sample while avoiding the use of the entire sample, and maintaining a correlation to the total sample. While our method of hemoglobin was efficient it also removed the biomarkers we wished to analyze. Thus, an alternative method is necessary to determine if removing …
Date: December 2014
Creator: Prado, Eric A.
System: The UNT Digital Library
Effects of Sertraline Exposure on Fathead Minnow (Pimephales promelas) Steroidogenesis (open access)

Effects of Sertraline Exposure on Fathead Minnow (Pimephales promelas) Steroidogenesis

Sertraline is a selective serotonin reuptake inhibitor (SSRI) that is widely used for the treatment of depression and anxiety. Due to the abundant therapeutic use of sertraline, low levels have been detected in municipal wastewater effluents suggesting that aquatic organisms may be exposed. The purpose of this study was to evaluate the steroidogenic effects of sertraline on larval (FHM) and adult female fathead minnows (FFHM), Pimephales promelas. Larval FHM were exposed to 0.1, 1, and 10 µg/L sertraline for 28 days and analyzed via RT-qPCR for differential expression of 11β-Hydroxysteroid dehydrogenase (11β-HSD), 20β-Hydroxysteroid dehydrogenase (20β-HSD), aromatase (CYP19), and nuclear thyroid receptor alpha (TRα). FFHM were exposed to 3 or 10 µg/L sertraline for 7 days with the brain and ovary excised at exposure termination. Juvenile FHM exposed to 0.1 μg/L sertraline had a significant upregulation of both 20β-HSD and TRα. FFHM exposed to 10 µg/L sertraline had a significant upregulation of 11β-HSD expression in brain tissue, while no steroidogenic changes were observed in the FFHM ovary. Similarly, in FFHM brain tissue, CYP19 and 20β-HSD expression levels were significantly higher in fish exposed to 10µg/L sertraline compared to control. The significance of these findings with respect to survival, growth and reproduction …
Date: December 2014
Creator: Carty, Dennis R.
System: The UNT Digital Library
The Perception and Viability of English Corner on the American Campus (open access)

The Perception and Viability of English Corner on the American Campus

International students are often under considerable pressure from language barriers, culture shock, social isolation and lack of social support in American universities. Those stressors often discourage international students as ESL learners from practicing English with native students on campus. Based on Krashen & Terrell’s subconscious acquisition and conscious learning hypothesis, Vygotsky’s Zone of Proximal Development , Cummins’ Basic Interpersonal Communication System (BICS) and Cognitive Academic Language Proficiency (CALP), Horwitz’s language anxiety and Oxford’s indirect learning strategies , this study explores how international students and American students, respectively, perceive English Corner and whether English Corner could be an applicable out-of-class learning environment for international students to practice English and socialize with American students on American campuses. English Corner refers to regular meetings that English learners in Mainland China voluntarily organize in public places to practice spoken English. A survey was conducted on language learning strategies, socialization, acculturation, autonomy and English Corner among international students and native students at the University of North Texas. The questionnaires were adapted from Oxford and Nyikos’ study as to what variables affect choice of language learning strategies, Iheanacho’s study as to how international students use the Morris Library at the University of Delaware and their perception …
Date: December 2014
Creator: Hu, Jiaying
System: The UNT Digital Library
Optimization of Novel Culturing and Testing Procedures for Acute Effects on Acartia Tonsa and Tisbe Biminiensis (open access)

Optimization of Novel Culturing and Testing Procedures for Acute Effects on Acartia Tonsa and Tisbe Biminiensis

Copepods comprise an ecologically important role in freshwater and marine ecosystems, which is why they are often considered an important ecotoxicological model organism. The International Organization for Standardization’s (ISO) 14669 protocol is the only guideline for the determination of acute toxicity in three European marine copepod species: Acartia tonsa. The goal of this project was to assess the feasibility of establishing and maintaining cultures of Acartia tonsa, as well as to refine current culturing and egg separation methods. Initial culture methodology proved difficult for consistent production of eggs and collection of nauplii. The development of an airlift system for the separation of eggs from nauplii and adults, based on size, successfully increased the availability of eggs, nauplii and adults. The sensitivity and relative conditions of the copepod species was assessed by running a series of 48h acute toxicity tests with the reference toxicants 3,5-dichlorophenol, 4,4’-methylenebis(2,6-di-tert-butylphenol. The acute 48 hour median lethal dose concentration (LC50), the no observed effect concentration (NOEC), and the lowest observed effect concentration (LOEC) was analyzed for the three reference compounds for of A. tonsa.
Date: December 2014
Creator: Ussery, Erin J.
System: The UNT Digital Library
Evidence for Multiple Functions of a Medicago Truncatula Transporter (open access)

Evidence for Multiple Functions of a Medicago Truncatula Transporter

Legumes play an important role in agriculture as major food sources for humans and as feed for animals. Bioavailable nitrogen is a limiting nutrient for crop growth. Legumes are important because they can form a symbiotic relationship with soil bacteria called rhizobia that results in nitrogen-fixing root nodules. In this symbiosis, rhizobia provide nitrogen to the legumes and the legumes provide carbon sources to the rhizobia. The Medicago truncatula NPF1.7/NIP/LATD gene is essential for root nodule development and also for proper development of root architecture. Work in our lab on the MtNPF1.7/MtNIP/LATD gene has established that it encodes a nitrate transporter and strongly suggests it has another function. Mtnip-1/latd mutants have pleiotropic defects, which are only partially explained by defects in nitrate transport. MtNPF1.7/NIP/LATD is a member of the large and diverse NPF/NRT1(PTR) transporter family. NPF/NRT1(PTR) members have been shown to transport other compounds in addition to nitrate: nitrite, amino acids, di- and tri-peptides, dicarboxylates, auxin, abscisic acid and glucosinolates. In Arabidopsis thaliana, the AtNPF6.3/NRT1.1( CHL1) transporter was shown to transport auxin as well as nitrate. Atchl1 mutants have defects in root architecture, which may be explained by defects in auxin transport and/or nitrate sensing. Considering the pleiotropic phenotypes observed …
Date: December 2014
Creator: Huang, Ying-Sheng
System: The UNT Digital Library
The Measurement and Enhancement of Rapport Between Behavioral Therapists and Children with Autism (open access)

The Measurement and Enhancement of Rapport Between Behavioral Therapists and Children with Autism

Rapport has been acknowledged as an important variable in therapeutic contexts. The current evaluation defined and assessed rapport quality between children with autism and behavioral therapists based on behavioral correlates. In addition, the author evaluated the effects of an operant discrimination training procedure to enhance rapport levels for therapists with low levels of rapport. More specifically, the current study evaluated: (a) if the discrimination training procedure would establish therapists’ social interactions as a discriminative stimulus and (b) if social interaction would function as a conditioned reinforcer for novel responses. Results suggest that the discrimination training procedure was successful in conditioning social interaction as a reinforcer for all child participants, and as a result, rapport increased.
Date: December 2014
Creator: Lapin, Carly Ilyse
System: The UNT Digital Library
Monitoring Monocyte Oxldl Phagocytosis As a Cardiovascular Disease Risk Factor Following a High-fat Meal (open access)

Monitoring Monocyte Oxldl Phagocytosis As a Cardiovascular Disease Risk Factor Following a High-fat Meal

Macrophage-derived foam cells play a predominant role in the deposition of arterial plaques during the early stages of atherosclerosis. The deposition of arterial plaques is known to be effected by several factors, including a person’s dietary habits. The consumption of a high-fat (>60% of calories from fat) meal is known to elevate serum LDL and triglycerides, which have been previously implicated in the formation pf foam cells. One limitation of current research models is that it is not possible to directly measure foam cells in vivo. Thus, the purpose of the present study was to validate the use of blood derived monocytes as a proxy measure of foam cells. In order to complete this objective, we evaluated monocyte oxLDL phagocytosis capacity following consumption of a high-fat meal. Eight men and women participated in the present study and venous blood samples were collected prior to the meal, 1-h, 3-h, and 5-h post-meal. Monocytes (CD14+/16- and CD14+/16+) were evaluated for adhesion molecule expression (CD11a, CD11b, and CD18), scavenger R (CD36) expression, and oxLDL phagocytosis using an image-based flow cytometry method developed in our laboratory for this purpose. Data was statistically analyzed for significance using a single-factor ANOVA with repeated measures and a …
Date: December 2014
Creator: Henning, Andrea L.
System: The UNT Digital Library
Evaluation of Ethanol’s Effects on the Biophysical Characteristics of Licking (open access)

Evaluation of Ethanol’s Effects on the Biophysical Characteristics of Licking

Alcohol use disorders are a public health issue related to adverse effects for individuals and society. A low level of response, or decreased sensitivity, to alcohol has been identified as a heritable risk factor for development of alcohol use disorders. One method for researching level of response to alcohol is through the use of rodent models, which are developed to mimic human conditions while eliminating barriers to conducting research with people. Current rodent models used to evaluate effects of ethanol on motor performance have been criticized for not being well matched to human tasks that measure level of change in body sway after alcohol consumption. This study looks at oromotor behavior as a potential alternative to gross motor performance in hopes of increasing correspondence between human and rodent measures of intoxication. To evaluate rodent oromotor performance a force transducer lickometer is used to measure several dimensions of licking behavior after administration of different concentrations of ethanol solution via gavage. Results show that force of licking is not sensitive to dose of ethanol. The total number of licks per session show dose related decreases and licking rhythm, evaluated by the length and distribution of interlick intervals, either increased or decreased for …
Date: December 2014
Creator: Stewart, Daryl Ellen
System: The UNT Digital Library