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Advanced Recombinant Manganese Peroxidase for Biosynthesis of Lignin Bioproducts, Phase I Final Report, STTR Grant #: DE-SC0007503.
The core purpose of this Phase I STTR was to evaluate the feasibility of a new method of producing a recombinant version of manganese peroxidase (MnP) enzyme. MnP is a potentially valuable enzyme for producing high value lignin products and also for industrial de-coloring operations such as biobleaching of pulp and color removal from textile dye effluents. This lignin-modifying enzyme is produced in small amounts by the native host, a white rot fungus. Previous work by Oregon State University developed a secreted recombinant version of the enzyme in the yeast Pichia pastoris. Unfortunately, the expression is barely moderate and the enzyme is heavily glycosylated, which inhibits purification. In this work, the gene for the enzyme is given a tag which targets production of the enzyme to the peroxisome. This is a promising approach since this location is also where heme and hydrogen peroxide are sequestered, which are both necessary cofactors for MnP. More than ten recombinant strains were constructed, verified, and expressed in the Pichia system. Constitutive (GAP) and methanol-induced promoters (AOX) were tried for peroxisomal targeted, cytosolic, and secreted versions of MnP. Only the secreted strains showed activity. The amount of expression was not significantly changed. The degree of …
Date:
December 13, 2012
Creator:
Beatty, Christopher; Kitner, Joshua; Lajoie, Curtis; McClain, Sean & Potochnik, Steve
Object Type:
Report
System:
The UNT Digital Library
Advanced Refrigerant-Based Cooling Technologies for Information and Communication Infrastructure (ARCTIC)
Faster, more powerful and dense computing hardware generates significant heat and imposes considerable data center cooling requirements. Traditional computer room air conditioning (CRAC) cooling methods are proving increasingly cost-ineffective and inefficient. Studies show that using the volume of room air as a heat exchange medium is wasteful and allows for substantial mixing of hot and cold air. Further, it limits cabinet/frame/rack density because it cannot effectively cool high heat density equipment that is spaced closely together. A more cost-effective, efficient solution for maximizing heat transfer and enabling higher heat density equipment frames can be accomplished by utilizing properly positioned âÃÂÃÂphase changeâÃÂàor âÃÂÃÂtwo-phaseâÃÂàpumped refrigerant cooling methods. Pumping low pressure, oil-free phase changing refrigerant through microchannel heat exchangers can provide up to 90% less energy consumption for the primary cooling loop within the room. The primary benefits of such a solution include reduced energy requirements, optimized utilization of data center space, and lower OPEX and CAPEX. Alcatel-Lucent recently developed a modular cooling technology based on a pumped two-phase refrigerant that removes heat directly at the shelf level of equipment racks. The key elements that comprise the modular cooling technology consist of the following. A pump delivers liquid refrigerant to finned …
Date:
December 13, 2012
Creator:
Salamon, Todd
Object Type:
Report
System:
The UNT Digital Library
The Albany News (Albany, Tex.), Vol. 137, No. 30, Ed. 1 Thursday, December 13, 2012
Weekly newspaper from Albany, Texas that includes local, state and national news along with advertising.
Date:
December 13, 2012
Creator:
Lucas, Melinda L.
Object Type:
Newspaper
System:
The Portal to Texas History
The Altus Times (Altus, Okla.), Vol. 113, No. 141, Ed. 1 Thursday, December 13, 2012
Daily newspaper from Altus, Oklahoma that includes local, state, and national news along with advertising.
Date:
December 13, 2012
Creator:
Bush, Michael
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
Archer County News (Archer City, Tex.), Vol. 104, No. 50, Ed. 1 Thursday, December 13, 2012
Weekly newspaper from Archer City, Texas that includes local, state and national news along with advertising.
Date:
December 13, 2012
Creator:
Phillips, Barbara
Object Type:
Newspaper
System:
The Portal to Texas History
The Baytown Sun (Baytown, Tex.), Vol. 92, No. 248, Ed. 1 Thursday, December 13, 2012
Daily newspaper from Baytown, Texas that includes local, state, and national news along with advertising.
Date:
December 13, 2012
Creator:
Yanelli, Adam
Object Type:
Newspaper
System:
The Portal to Texas History
A Best Fit Approach to Estimating Multiple Diffuse Source Terms Using Ambient Air Monitoring Data and an Air Dispersion Model
None
Date:
December 13, 2012
Creator:
MacQueen, D. H.; Bertoldo, N. A. & Wegrecki, A. M.
Object Type:
Article
System:
The UNT Digital Library
Biotic Processes Regulating the Carbon Balance of Desert Ecosystems - Final Report
Our results from the 10-year elevated atmospheric CO{sub 2} concentration study at the Nevada Desert FACE (Free-air CO{sub 2} Enrichment) Facility (NDFF) indicate that the Mojave Desert is a dynamic ecosystem with the capacity to respond quickly to environmental changes. The Mojave Desert ecosystem is accumulating carbon (C), and over the 10-year experiment, C accumulation was significantly greater under elevated [CO{sub 2}] than under ambient, despite great fluctuations in C inputs from year to year and even apparent reversals in which [CO{sub 2}] treatment had greater C accumulations.
Date:
December 13, 2012
Creator:
Nowak, Robert S; Smith, Stanley D; Evans, Dave; Ogle, Kiona & Fenstermaker, Lynn
Object Type:
Report
System:
The UNT Digital Library
The Birth of a Galaxy: Primordial Metal Enrichment and Stellar Populations
None
Date:
December 13, 2012
Creator:
Wise, John H.; Turk, Matthew J.; Norman, Michael L.; Abel, Tom & /KIPAC, Menlo Park
Object Type:
Article
System:
The UNT Digital Library
Branching Fraction and Form-factor Shape Measurements of Exclusive Charmless Semileptonic B Decays, and Determination of |Vub|
None
Date:
December 13, 2012
Creator:
Lees, J. P.; Poireau, V.; Tisserand, V.; Garra Tico, J.; Grauges, E.; Palano, A. et al.
Object Type:
Article
System:
The UNT Digital Library
Brownwood Bulletin (Brownwood, Tex.), Vol. 113, No. 51, Ed. 1 Thursday, December 13, 2012
Weekly newspaper from Brownwood, Texas that includes local, state and national news along with advertising.
Date:
December 13, 2012
Creator:
Stuckly, Derrick
Object Type:
Newspaper
System:
The Portal to Texas History
The Canadian Record (Canadian, Tex.), Vol. 122, No. 50, Ed. 1 Thursday, December 13, 2012
Weekly newspaper from Canadian, Texas that includes local, state and national news along with some advertising.
Date:
December 13, 2012
Creator:
Brown, Laurie Ezzell
Object Type:
Newspaper
System:
The Portal to Texas History
Canton Herald (Canton, Tex.), Vol. 131, No. 2, Ed. 1 Thursday, December 13, 2012
Weekly newspaper from Canton, Texas that includes local, state and national news along with some advertising.
Date:
December 13, 2012
Creator:
Vaughan, Julie
Object Type:
Newspaper
System:
The Portal to Texas History
The Cleburne Eagle News (Cleburne, Tex.), Ed. 1 Thursday, December 13, 2012
Weekly newspaper from Cleburne, Texas that includes local, state, and national news along with advertising.
Date:
December 13, 2012
Creator:
Oaks, Judy & Oaks, Kelly
Object Type:
Newspaper
System:
The Portal to Texas History
Comanche Chief (Comanche, Tex.), No. 34, Ed. 1 Thursday, December 13, 2012
Weekly newspaper from Comanche, Texas that includes local, state and national news along with advertising.
Date:
December 13, 2012
Creator:
Wilkerson, James C., III
Object Type:
Newspaper
System:
The Portal to Texas History
COMPILATION AND MANAGEMENT OF ORP GLASS FORMULATION DATABASE, VSL-12R2470-1 REV 0
The present report describes the first steps in the development of a glass property-composition database for WTP LAW and HL W glasses that includes all of the data that were used in the development of the WTP baseline models and all of the data collected subsequently as part of WTP enhancement studies perfonned for ORP. The data were reviewed to identifY some of the more significant gaps in the composition space that will need to be filled to support waste processing at Hanford. The WTP baseline models have been evaluated against the new data in terms of range of validity and prediction perfonnance.
Date:
December 13, 2012
Creator:
Kruger, Albert A.; Pasieka, Holly K.; Muller, Isabelle; Gilbo, Konstantin; Perez-Cardenas, Fernando; Joseph, Innocent et al.
Object Type:
Report
System:
The UNT Digital Library
The Controlled Substances Act: Regulatory Requirements
This report highlights certain non-criminal regulatory requirements of the Controlled Substances Act(CSA).
Date:
December 13, 2012
Creator:
Yeh, Brian T.
Object Type:
Report
System:
The UNT Digital Library
Cooper Review (Cooper, Tex.), Vol. 132, No. 50, Ed. 1 Thursday, December 13, 2012
Weekly newspaper from Cooper, Texas that includes local, state, and national news along with advertising.
Date:
December 13, 2012
Creator:
Roller, Cindy
Object Type:
Newspaper
System:
The Portal to Texas History
Crowley Star (Crowley, Tex.), Vol. 26, No. 32, Ed. 1 Thursday, December 13, 2012
Weekly newspaper from Crowley, Texas that includes local, state, and national news along with advertising.
Date:
December 13, 2012
Creator:
Hinton, Jay
Object Type:
Newspaper
System:
The Portal to Texas History
A Demonstration of the Disposal Systems Evaluation Framework (DSEF)
None
Date:
December 13, 2012
Creator:
Greenberg, H. R.; Blink, J. A.; Sharma, M. & Sutton, M.
Object Type:
Article
System:
The UNT Digital Library
Design and Characterization of a Prototype Stripline Beam Position Monitor for the Clic Drive Beam*
None
Date:
December 13, 2012
Creator:
Benot-Morell, A.; Soby, L.; Wendt, M.; Nappa, J. M.; Tassan-Viol, J.; Vilalte, S. et al.
Object Type:
Article
System:
The UNT Digital Library
Determination of the Impact of Glycolate on ARP and MCU Operations
Savannah River Remediation (SRR) is evaluating an alternate flowsheet for the Defense Waste Processing Facility (DWPF) using glycolic acid as a reductant. An important aspect of the development of the glycolic acid flowsheet is determining if glycolate has any detrimental downstream impacts. Testing was performed to determine if there is any impact to the strontium and actinide sorption by monosodium titanate (MST) and modified monosodium titanate (mMST) or if there is an impact to the cesium removal, phase separation, or coalescer performance at the Modular Caustic-Side Solvent Extraction Processing Unit (MCU). Sorption testing was performed using both MST and modified MST (mMST) in the presence of 5000 and 10,000 ppm (mass basis) glycolate. 10,000 ppm is the estimated bounding concentration expected in the DWPF recycle stream based on DWPF melter flammable gas model results. The presence of glycolate was found to slow the removal of Sr and Pu by MST, while increasing the removal rate of Np. Results indicate that the impact is a kinetic effect, and the overall capacity of the material is not affected. There was no measurable effect on U removal at either glycolate concentration. The slower removal rates for Sr and Pu at 5000 and 10,000 …
Date:
December 13, 2012
Creator:
Taylor-Pashow, K. M.L.; Peters, T. B.; Fondeur, F. F.; Shehee, T. C. & Washington, A. L.
Object Type:
Report
System:
The UNT Digital Library
Development Of High Waste-Loading HLW Glasses For High Bismuth Phosphate Wastes, VSL-12R2550-1, Rev 0
This report presents results from tests with new glass formulations that have been developed for several high Bi-P HLW compositions that are expected to be processed at the WTP that have not been tested previously. WTP HLW feed compositions were reviewed to select waste batches that are high in Bi-P and that are reasonably distinct from the Bi-limited waste that has been tested previously. Three such high Bi-P HLW compositions were selected for this work. The focus of the present work was to determine whether the same type of issues as seen in previous work with high-Bi HLW will be seen in HLW with different concentrations of Bi, P and Cr and also whether similar glass formulation development approaches would be successful in mitigating these issues. New glass compositions were developed for each of the three representative Bi-P HLW wastes and characterized with respect to key processing and product quality properties and, in particular, those relating to crystallization and foaming tendency.
Date:
December 13, 2012
Creator:
Kruger, A. A.; Pegg, Ian L.; Gan, Hao & Kot, Wing K.
Object Type:
Report
System:
The UNT Digital Library
Drug Offenses: Maximum Fines and Terms of Imprisonment for Violation of the Federal Controlled Substances Act and Related Laws
This report is a chart of the maximum fines and terms of imprisonment that may be imposed as a consequence of conviction for violation of the federal Controlled Substances Act (CSA) and other drug supply and drug demand related laws.
Date:
December 13, 2012
Creator:
Yeh, Brian T.
Object Type:
Report
System:
The UNT Digital Library