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ChIP-seq Identification of Weakly Conserved Heart Enhancers
Accurate control of tissue-specific gene expression plays a pivotal role in heart development, but few cardiac transcriptional enhancers have thus far been identified. Extreme non-coding sequence conservation successfully predicts enhancers active in many tissues, but fails to identify substantial numbers of heart enhancers. Here we used ChIP-seq with the enhancer-associated protein p300 from mouse embryonic day 11.5 heart tissue to identify over three thousand candidate heart enhancers genome-wide. Compared to other tissues studied at this time-point, most candidate heart enhancers are less deeply conserved in vertebrate evolution. Nevertheless, the testing of 130 candidate regions in a transgenic mouse assay revealed that most of them reproducibly function as enhancers active in the heart, irrespective of their degree of evolutionary constraint. These results provide evidence for a large population of poorly conserved heart enhancers and suggest that the evolutionary constraint of embryonic enhancers can vary depending on tissue type.
Date:
July 1, 2010
Creator:
Blow, Matthew J.; McCulley, David J.; Li, Zirong; Zhang, Tao; Akiyama, Jennifer A.; Holt, Amy et al.
Object Type:
Article
System:
The UNT Digital Library
Alloy Design and Thermomechanical Processing of a Beta Titanium Alloy for a Heavy Vehicle Application
With the strength of steel, but at half the weight, titanium has the potential to offer significant benefits in the weight reduction of heavy vehicle components while possibly improving performance. However, the cost of conventional titanium fabrication is a major barrier in implementation. New reduction technologies are now available that have the potential to create a paradigm shift in the way the United States uses titanium, and the economics associated with fabrication of titanium components. This CRADA project evaluated the potential to develop a heavy vehicle component from titanium powders. The project included alloy design, development of manufacturing practices, and modeling the economics associated with the new component. New Beta alloys were designed for this project to provide the required mechanical specifications while utilizing the benefits of the new fabrication approach. Manufacturing procedures were developed specific to the heavy vehicle component. Ageing and thermal treatment optimization was performed to provide the desired microstructures. The CRADA partner established fabrication practices and targeted capital investment required for fabricating the component out of titanium. Though initial results were promising, the full project was not executed due to termination of the effort by the CRADA partner and economic trends observed in the heavy vehicle …
Date:
July 2, 2010
Creator:
Blue, Craig A. & Peter, William H.
Object Type:
Report
System:
The UNT Digital Library
Hurricane Intensity, Sea Surface Temperature, and Stochastic Variation
None
Date:
July 14, 2010
Creator:
Bogen, K. T.; Fischer, L. E. & Jones, E. D.
Object Type:
Article
System:
The UNT Digital Library
Oral History Interview with Walter E. Bolssen, July 29, 2010
Transcript of an oral interview with Walter E. Bolssen. Born in 1918, he enlisted in the Navy in December, 1939. After training as an airplane engine mechanic, he was sent to Kaneohe Bay, Hawaii in late 1940. He served as a PBY crew plane captain in VP-12. He describes his experiences during and after the attack on Pearl Harbor. In 1943 he was stationed at Henderson Field on Guadalcanal where he flew on night patrols in a “Black Cat” PBY-5. After approximately one year on Guadalcanal, he was sent to New Orleans, Louisiana, where he trained mechanics for the “Yellow Peril” N3N airplane. After the war ended, he was discharged from the Navy, but re-enlisted three months later. He retired in 1960.
Date:
July 29, 2010
Creator:
Bolssen, Walter E.
Object Type:
Sound
System:
The Portal to Texas History
Oral History Interview with Walter E. Bolssen, July 29, 2010
Transcript of an oral interview with Walter E. Bolssen. Born in 1918, he enlisted in the Navy in December, 1939. After training as an airplane engine mechanic, he was sent to Kaneohe Bay, Hawaii in late 1940. He served as a PBY crew plane captain in VP-12. He describes his experiences during and after the attack on Pearl Harbor. In 1943 he was stationed at Henderson Field on Guadalcanal where he flew on night patrols in a “Black Cat” PBY-5. After approximately one year on Guadalcanal, he was sent to New Orleans, Louisiana, where he trained mechanics for the “Yellow Peril” N3N airplane. After the war ended, he was discharged from the Navy, but re-enlisted three months later. He retired in 1960.
Date:
July 29, 2010
Creator:
Bolssen, Walter E.
Object Type:
Text
System:
The Portal to Texas History
Test Loop Demonstration and Evaluation of Slurry Transfer Line Critical Velocity Measurement Instruments
This report presents the results of the evaluation of three ultrasonic sensors for detecting critical velocity during slurry transfer between the Hanford tank farms and the WTP.
Date:
July 31, 2010
Creator:
Bontha, Jagannadha R.; Jenks, Jeromy WJ; Morgen, Gerald P.; Peters, Timothy J.; Wilcox, Wayne A.; Adkins, Harold E. et al.
Object Type:
Report
System:
The UNT Digital Library
Pair-distribution function analysis of the structural valence transition in Cp{sub 2}{sup *}Yb(4,4'-Me{sub 2}-bipy)
The Cp{sup ∗}{sub 2} Yb(L) class of compounds, where Cp{sup ∗}=pentamethylcyclopentadienyl = C{sub 5}Me{sub 5} and L is either a 1,4-diazabutadiene or bipy = 2,2′-bipyridine related ligand, have provided excellent analogies to the Kondo state on the nanoscale. Cp{sup ∗}{sub 2} Yb(4,4′-Me{sub 2}-bipy) furthers this analogy by demonstrating a valence transition as the sample is cooled below 200 K. Here, pair-distribution function (PDF) analysis of x-ray powder diffraction data demonstrate that the Cp{sup ∗}{sub 2}Yb(4,4′-Me{sub 2}-bipy) molecule is virtually unchanged through the valence transition. However, the molecule’s stacking arrangement is altered through the valence transition.
Date:
July 20, 2010
Creator:
Booth, C H; Bauer, E D; Bozin, E S; Billinge, S J L & Walter, M D
Object Type:
Article
System:
The UNT Digital Library
Fabrication and Test of 90-mm Nb3Sn Quadrupole Model Based on Dipole-type Collar
A series of 90-mm TQC quadrupole models with a collar-based mechanical structure has been fabricated and tested within the framework of the US-LHC Accelerator Research Program (LARP) using quadrupole-symmetric stainless steel collar laminations. This paper describes the design features, construction and test of TQC02Eb, the first TQC made with dipole-type collar and collaring techniques. Magnet test includes quench performance and field quality measurements at 4.5 and 1.9 K. Results of model performance for TQC quadrupoles based on dipole-type and quadrupole-type collars are compared and discussed.
Date:
July 29, 2010
Creator:
Bossert, R.; Andreev, N.; Chlachidze, G.; Kashikhin, V. S.; Kashikhin, V. V.; Lamm, M. J. et al.
Object Type:
Article
System:
The UNT Digital Library
Radiation leukaemogenesis at low doses DE-FG02-05 ER 63947 Final Technical Report 15 May 2005 ÃÂÃÂÃÂÃÂÃÂÃÂÃÂâÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂà14 May 2010
This report provides a complete summary of the work undertaken and results obtained under US Department of Energy grant DF-FG02-05 ER 63947, Radiation leukaemogenesis at low doses. There is ample epidemiological evidence indicating that ionizing radiation is carcinogenic in the higher dose range. This evidence, however, weakens and carries increasing uncertainties at doses below 100-200 mSv. At these low dose levels the form of the dose-response curve for radiation-induced cancer cannot be determined reliably or directly from studies of human populations. Therefore animal, cellular and other experimental systems must be employed to provide supporting evidence on which to base judgements of risk at low doses. Currently in radiological protection a linear non-threshold (LNT) extrapolation of risk estimates derived from human epidemiological studies is used to estimate risks in the dose range of interest for protection purposes. Myeloid leukaemias feature prominently among the cancers associated with human exposures to ionising radiation (eg UNSCEAR 2006; IARC 2000). Good animal models of radiation-induced acute myeloid leukaemia (AML) are available including strains such as CBA, RFM and SJL (eg Major and Mole 1978; Ullrich et al 1976; Resnitzky et al 1985). Early mechanistic studies using cytogenetic methods in these mouse models established that the …
Date:
July 28, 2010
Creator:
Bouffler, Simon
Object Type:
Report
System:
The UNT Digital Library
Community Development Block Grant Funds in Disaster Relief and Recovery
This report discusses how Community Development Block Grant (CDBG) programs are funded by Congress and what they have been used for in recent years: recovery efforts following terrorist attacks, riots, and natural disasters. The 111th Congress is considering $100 million in CDBG funds to help states and communities undertake disaster recovery activities in presidentially declared disaster areas affected by severe storms and flooding during the period from March 2010 through May 2010. The act limited distribution of these funds to states where the entire state was declared a disaster area (Rhode Island) and to states where at least 20 counties within the state were declared disaster areas (Tennessee, Kentucky, and Nebraska).
Date:
July 12, 2010
Creator:
Boyd, Eugene
Object Type:
Report
System:
The UNT Digital Library
Control of Toxic Chemicals in Puget Sound, Phase 3: Study of Atmospheric Deposition of Air Toxics to the Surface of Puget Sound
The results of the Phase 1 Toxics Loading study suggested that runoff from the land surface and atmospheric deposition directly to marine waters have resulted in considerable loads of contaminants to Puget Sound (Hart Crowser et al. 2007). The limited data available for atmospheric deposition fluxes throughout Puget Sound was recognized as a significant data gap. Therefore, this study provided more recent or first reported atmospheric deposition fluxes of PAHs, PBDEs, and select trace elements for Puget Sound. Samples representing bulk atmospheric deposition were collected during 2008 and 2009 at seven stations around Puget Sound spanning from Padilla Bay south to Nisqually River including Hood Canal and the Straits of Juan de Fuca. Revised annual loading estimates for atmospheric deposition to the waters of Puget Sound were calculated for each of the toxics and demonstrated an overall decrease in the atmospheric loading estimates except for polybrominated diphenyl ethers (PBDEs) and total mercury (THg). The median atmospheric deposition flux of total PBDE (7.0 ng/m2/d) was higher than that of the Hart Crowser (2007) Phase 1 estimate (2.0 ng/m2/d). The THg was not significantly different from the original estimates. The median atmospheric deposition flux for pyrogenic PAHs (34.2 ng/m2/d; without TCB) shows …
Date:
July 5, 2010
Creator:
Brandenberger, Jill M.; Louchouarn, Patrick; Kuo, Li-Jung; Crecelius, Eric A.; Cullinan, Valerie I.; Gill, Gary A. et al.
Object Type:
Report
System:
The UNT Digital Library
Initial inclusion of thermodynamic considerations in Kayenta.
A persistent challenge in simulating damage of natural geological materials, as well as rock-like engineered materials, is the development of efficient and accurate constitutive models. The common feature for these brittle and quasi-brittle materials are the presence of flaws such as porosity and network of microcracks. The desired models need to be able to predict the material responses over a wide range of porosities and strain rate. Kayenta (formerly called the Sandia GeoModel) is a unified general-purpose constitutive model that strikes a balance between first-principles micromechanics and phenomenological or semi-empirical modeling strategies. However, despite its sophistication and ability to reduce to several classical plasticity theories, Kayenta is incapable of modeling deformation of ductile materials in which deformation is dominated by dislocation generation and movement which can lead to significant heating. This stems from Kayenta's roots as a geological model, where heating due to inelastic deformation is often neglected or presumed to be incorporated implicitly through the elastic moduli. The sophistication of Kayenta and its large set of extensive features, however, make Kayenta an attractive candidate model to which thermal effects can be added. This report outlines the initial work in doing just that, extending the capabilities of Kayenta to include …
Date:
July 1, 2010
Creator:
Brannon, Rebecca Moss (University of Utah, Salt Lake City, UT); Bishop, Joseph E.; Fuller, Timothy J. (University of Utah, Salt Lake City, UT) & Strack, Otto Eric
Object Type:
Report
System:
The UNT Digital Library
Demonstrating Structural Adequacy of Nuclear Power Plant Containment Structures for Beyond Design-Basis Pressure Loadings
ABSTRACT Demonstrating the structural integrity of U.S. nuclear power plant (NPP) containment structures, for beyond design-basis internal pressure loadings, is necessary to satisfy Nuclear Regulatory Commission (NRC) requirements and performance goals. This paper discusses methods for demonstrating the structural adequacy of the containment for beyond design-basis pressure loadings. Three distinct evaluations are addressed: (1) estimating the ultimate pressure capacity of the containment structure (10 CFR 50 and US NRC Standard Review Plan, Section 3.8) ; (2) demonstrating the structural adequacy of the containment subjected to pressure loadings associated with combustible gas generation (10 CFR 52 and 10 CFR 50); and (3) demonstrating the containment structural integrity for severe accidents (10 CFR 52 as well as SECY 90-016, SECY 93-087, and related NRC staff requirements memoranda (SRMs)). The paper describes the technical basis for specific aspects of the methods presented. It also presents examples of past issues identified in licensing activities related to these evaluations.
Date:
July 18, 2010
Creator:
Braverman, J. I. & Morante, R.
Object Type:
Article
System:
The UNT Digital Library
Key Physical Mechanisms in Nanostructured Solar Cells
The objective of the project was to study both theoretically and experimentally the excitation, recombination and transport properties required for nanostructured solar cells to deliver energy conversion efficiencies well in excess of conventional limits. These objectives were met by concentrating on three key areas, namely, investigation of physical mechanisms present in nanostructured solar cells, characterization of loss mechanisms in nanostructured solar cells and determining the properties required of nanostructured solar cells in order to achieve high efficiency and the design implications.
Date:
July 21, 2010
Creator:
Bremner, Stephan
Object Type:
Report
System:
The UNT Digital Library
Media and Communication
This book chapter discusses media and communication. As core features of humanity, communication and media clearly predate academic disciplines. They are in this sense non-disciplinary. Yet, they have for centuries been the subject of inquiry by those concerned to understand and improve human correspondence. This chapter surveys the historical development and present form of multi-, inter-, and transdisciplinary studies of media and communication.
Date:
July 2010
Creator:
Briggle, Adam & Christians, Clifford G.
Object Type:
Book Chapter
System:
The UNT Digital Library
The Stability and Electrical Properties of High Temperature Proton Conductors
The morphological and electrical properties of Ba{sub 1-x}Sr{sub x}Ce{sub 0.8}Y{sub 0.2}O{sub 3-{delta}} with x varying from 0 to 1 prepared by a modified Pechini method were investigated as potential high temperature proton conductors. Dense microstructures were achieved for all the samples upon sintering at 1500 C for 5 h. The phase structure analysis indicated that perovskite phase was formed for 0 {le} x {le} 0.2, while for x larger than 0.5, impurity phases of Sr{sub 2}CeO{sub 4} and Y{sub 2}O{sub 3} appeared. The tolerance to H{sub 2}O for the samples improved with the increase in Sr content when exposed to boiling water, while the electrical conductivity decreased from x = 0 to 1. However, the resistance to CO{sub 2} attack at elevated temperatures was not improved within the whole x range studied.
Date:
July 6, 2010
Creator:
Brinkman, K.
Object Type:
Article
System:
The UNT Digital Library
Detroit Commuter Hydrogen Project
This project was undertaken to demonstrate the viability of using hydrogen as a fuel in an internal combustion engine vehicle for use as a part of a mass transit system. The advantages of hydrogen as a fuel include renew-ability, minimal environmental impact on air quality and the environment, and potential to reduce dependence on foreign energy sources for the transportation sector. Recognizing the potential for the hydrogen fuel concept, the Southeast Michigan Congress of Governments (SEMCOG) determined to consider it in the study of a proposed regional mass transit rail system for southeast Michigan. SEMCOG wanted to evaluate the feasibility of using hydrogen fueled internal combustion engine (H2ICE) vehicles in shuttle buses to connect the Detroit Metro Airport to a proposed, nearby rail station. Shuttle buses are in current use on the airport for passenger parking and inter-terminal transport. This duty cycle is well suited to the application of hydrogen fuel at this time because of the ability to re-fuel vehicles at a single nearby facility, overcoming the challenge of restricted fuel availability in the undeveloped hydrogen fuel infrastructure. A cooperative agreement between SEMCOG and the DOE was initiated and two H2ICE buses were placed in regular passenger service on …
Date:
July 31, 2010
Creator:
Brooks, Jerry & Prebo, Brendan
Object Type:
Report
System:
The UNT Digital Library
Perry Daily Journal (Perry, Okla.), Vol. 118, No. 127, Ed. 1 Thursday, July 1, 2010
Daily newspaper from Perry, Oklahoma that includes local, state, and national news along with advertising.
Date:
July 1, 2010
Creator:
Brown, Gloria
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
Perry Daily Journal (Perry, Okla.), Vol. 118, No. 128, Ed. 1 Friday, July 2, 2010
Daily newspaper from Perry, Oklahoma that includes local, state, and national news along with advertising.
Date:
July 2, 2010
Creator:
Brown, Gloria
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
Perry Daily Journal (Perry, Okla.), Vol. 118, No. 129, Ed. 1 Saturday, July 3, 2010
Daily newspaper from Perry, Oklahoma that includes local, state, and national news along with advertising.
Date:
July 3, 2010
Creator:
Brown, Gloria
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
Perry Daily Journal (Perry, Okla.), Vol. 118, No. 130, Ed. 1 Tuesday, July 6, 2010
Daily newspaper from Perry, Oklahoma that includes local, state, and national news along with advertising.
Date:
July 6, 2010
Creator:
Brown, Gloria
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
Perry Daily Journal (Perry, Okla.), Vol. 118, No. 131, Ed. 1 Wednesday, July 7, 2010
Daily newspaper from Perry, Oklahoma that includes local, state, and national news along with advertising.
Date:
July 7, 2010
Creator:
Brown, Gloria
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
Perry Daily Journal (Perry, Okla.), Vol. 118, No. 132, Ed. 1 Thursday, July 8, 2010
Daily newspaper from Perry, Oklahoma that includes local, state, and national news along with advertising.
Date:
July 8, 2010
Creator:
Brown, Gloria
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
Perry Daily Journal (Perry, Okla.), Vol. 118, No. 133, Ed. 1 Friday, July 9, 2010
Daily newspaper from Perry, Oklahoma that includes local, state, and national news along with advertising.
Date:
July 9, 2010
Creator:
Brown, Gloria
Object Type:
Newspaper
System:
The Gateway to Oklahoma History