Degree Level

States

184 Matching Results

Results open in a new window/tab.

Validation of the Spanish SIRS: Beyond Linguistic Equivalence in the Assessment of Malingering among Spanish Speaking Clinical Populations (open access)

Validation of the Spanish SIRS: Beyond Linguistic Equivalence in the Assessment of Malingering among Spanish Speaking Clinical Populations

Malingering is the deliberate production of feigned symptoms by a person seeking external gain such as: financial compensation, exemption from duty, or leniency from the criminal justice system. The Test Translation and Adaptation Guidelines developed by the International Test Commission (ITC) specify that only tests which have been formally translated into another language and validated should be available for use in clinical practice. Thus, the current study evaluated the psychometric properties of a Spanish translation of the Structured Interview of Reported Symptoms (SIRS). Using a simulation design with 80 Spanish-speaking Hispanic American outpatients, the Spanish SIRS was produced reliable results with small standard errors of measurement (SEM). Regarding discriminant validity, very large effect sizes (mean Cohen's d = 2.00) were observed between feigners and honest responders for the SIRS primary scales. Research limitations and directions for future research are also discussed.
Date: August 2010
Creator: Correa, Amor Alicia
Object Type: Thesis or Dissertation
System: The UNT Digital Library
The Influence of Income, Ethnicity, and Parenting on Child Health (open access)

The Influence of Income, Ethnicity, and Parenting on Child Health

Children in low-income and ethnic minority families are more likely to be in poor health, which may impact physical and economic well-being in adulthood. This study explored how maternal depression and parenting efficacy were associated with child health outcomes in a sample of low-income African American and Latino families. Results demonstrated that children in optimal health were more likely to have mothers with high parenting efficacy and fewer depressive symptoms. Differences between African American and Latino families illustrated the importance of considering both socioeconomic and racial and ethnic disparities in child health simultaneously. Parent characteristics may be opportune targets for addressing child health disparities, and future research should focus on understanding these associations and identifying parent behaviors associated with child health.
Date: August 2010
Creator: Dier, Shannon E.
Object Type: Thesis or Dissertation
System: The UNT Digital Library
The Pure Rotational Spectra of Diatomics and Halogen-Addition Benzene Measured by Microwave and Radio Frequency Spectrometers (open access)

The Pure Rotational Spectra of Diatomics and Halogen-Addition Benzene Measured by Microwave and Radio Frequency Spectrometers

Two aluminum spherical mirrors with radii of 203.2 mm and radii of curvature also of 203.2 mm have been used to construct a tunable Fabry-Perót type resonator operational at frequencies as low as 500 MHz. The resonator has been incorporated into a pulsed nozzle, Fourier transform, Balle-Flygare spectrometer. The spectrometer is of use in recording low J transitions of large asymmetric molecules where the spectra are often greatly simplified compared to higher frequency regions. The resonators use is illustrated by recording the rotational spectra of bromobenzene and iodobenzene. In related experiments, using similar equipment, the pure rotational spectra of four isotopomers of SrS and all three naturally occurring isotopomers of the actinide-containing compound thorium monoxide have been recorded between 6 and 26 GHz. The data have been thoroughly analyzed to produce information pertaining to bond lengths and electronic structures.
Date: August 2010
Creator: Etchison, Kerry C.
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Le Nuove Musiche: Giovanni Battista Bovicelli? (open access)

Le Nuove Musiche: Giovanni Battista Bovicelli?

This thesis is a comparative study on the late 16th century manuals of ornamentation by Girolamo Dalla Casa, Giovanni Bassano, Riccardo Rognoni, and Giovanni Battista Bovicelli. The study demonstrates that the latest Renaissance manual should be given more credit for the innovative ornamentation style that was to come in the Early Baroque era. Bovicelli's use of sequence, dissonances, and less moving notes for more rhythmic varieties are features most often associated in the style of the Baroque. Unfortunately, the topic of ornamentation in the late Renaissance is most commonly discussed as a group of different entities writing in the same style. The research for this paper is intended to separate the manuals of the late Renaissance, focusing on the separate styles that led to the work of Giovanni Battista Bovicelli.
Date: August 2010
Creator: Gámez Hernández, Carlos
Object Type: Thesis or Dissertation
System: The UNT Digital Library
An Observation of Early Parent-Infant Social Interactions in Relation to the Emergence of Joint Attention in the Natural Environment (open access)

An Observation of Early Parent-Infant Social Interactions in Relation to the Emergence of Joint Attention in the Natural Environment

Early interactions between parents and infants are thought to be critical of later development. In particular joint attention has been an area of research and investigations. This study sought to measure joint attention behaviors in infants from 5 to 33 weeks of age under naturalistic conditions: in the home with the mother as the interaction partner given no instructions. Videotapes of the infant-parent interactions were observed and data were collected on behaviors related to joint attention. Given observations occur at younger ages than other studies considered, engagement data results indicate increasing trends for 3 of the 5 infants observed while the direction of infant gaze results indicate patterns consistent with descriptions currently in the literature. Parent behavior data indicate high levels of support in engaging infant attention. Furthering an understanding of joint attention by observing at earlier ages in infant development may be useful in informing teaching programs for infants who have not developed joint attention skills.
Date: May 2010
Creator: Pinsky, Karen
Object Type: Thesis or Dissertation
System: The UNT Digital Library
"He's a Human, You're a Mermaid": Narrative Performance in Disney's The Little Mermaid (open access)

"He's a Human, You're a Mermaid": Narrative Performance in Disney's The Little Mermaid

Disney animation represents a powerful source of economic and cultural production. However, following the death of Walt Disney, the animation division found itself struggling to survive. It was not until the 1989 release of the hugely successful animated film The Little Mermaid that Disney would reclaim its domination among children's cultural producers. Additionally, The Little Mermaid inaugurated a shift in Disney's portrayals of gender as the company replaced the docile passive princess characteristic of its previous animated films with a physically active and strong willed ambitious heroine. Grounded in an understanding of Disney's cultural significance as dominant storyteller, the present study explores gender in The Little Mermaid by means of narrative performativity. Specifically, I analyze the film's songs "Part of Your World," "Under the Sea," and "Poor Unfortunate Souls" as metonymic narrative performances of gender that are (1) embodied, (2) materially situated, (3) discursively embedded and (4) capable of legitimating and critiquing existing power relations.
Date: May 2010
Creator: Polanco, Raquel
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Family Sex Talk: Analyzing the Influence of Family Communication Patterns on Parent and Late Adolescent's Sex Conversations (open access)

Family Sex Talk: Analyzing the Influence of Family Communication Patterns on Parent and Late Adolescent's Sex Conversations

Family communication has the potential to affect a variety of youth behavioral outcomes including adolescent sexual risk behavior. Within chapter 1, I present past literature on adolescent sexual risk behaviors, family communication patterns, and the gaps associated with those areas. In chapter 2, I review previous literature on adolescent sexual risk behavior, parent-child communication and family communication patterns. In chapter 3, I present the method which includes a description of the participants, procedures, measures, and data analysis used. In Chapter 4, I present the results of the study. According to the results of the study, father-child communication is not a better predictor of adolescent sexual risk behavior. A higher quantity of parent-child communication does not lead to less adolescent sexual risk behavior. Participants with a pluralistic family type do significantly differ from laissez-faire and protective family types in regards to levels of parent-child communication. Participants with a consensual family type do have significantly higher levels of parent-child communication in comparison to laissez-faire family types, but not protective family types. Finally, in chapter 5, I present the discussion with a review of previous research (consistent or inconsistent with the current findings), limitations and conclusions for the current study.
Date: August 2010
Creator: Allen, Evette L.
Object Type: Thesis or Dissertation
System: The UNT Digital Library
A Spatially Explicit Environmental Health Surveillance Framework for Tick-Borne Diseases (open access)

A Spatially Explicit Environmental Health Surveillance Framework for Tick-Borne Diseases

In this paper, I will show how applying a spatially explicit context to an existing environmental health surveillance framework is vital for more complete surveillance of disease, and for disease prevention and intervention strategies. As a case study to test the viability of a spatial approach to this existing framework, the risk of human exposure to Lyme disease will be estimated. This spatially explicit framework divides the surveillance process into three components: hazard surveillance, exposure surveillance, and outcome surveillance. The components will be used both collectively and individually, to assess exposure risk to infected ticks. By utilizing all surveillance components, I will identify different areas of risk which would not have been identified otherwise. Hazard surveillance uses maximum entropy modeling and geographically weighted regression analysis to create spatial models that predict the geographic distribution of ticks in Texas. Exposure surveillance uses GIS methods to estimate the risk of human exposures to infected ticks, resulting in a map that predicts the likelihood of human-tick interactions across Texas, using LandScan 2008TM population data. Lastly, outcome surveillance uses kernel density estimation-based methods to describe and analyze the spatial patterns of tick-borne diseases, which results in a continuous map that reflects disease rates based …
Date: August 2010
Creator: Aviña, Aldo
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Effects of Reinforcer Magnitude on a Fixed Time Food Delivery Treatment of Pica (open access)

Effects of Reinforcer Magnitude on a Fixed Time Food Delivery Treatment of Pica

The purpose of the current study was to examine the effects of using fixed time schedules with different magnitudes of stimulus delivery as treatment for pica. A functional assessment was conducted, which indicated that pica occurred across experimental conditions and was most frequent in the absence of social stimulation or contingencies. A competing stimulus assessment was then conducted to identify stimuli that could potentially compete with pica during NCR. Subsequently, an evaluation of the effects of reinforcer magnitude on NCR as a treatment of pica was conducted. Treatment results indicated that quantity of reinforcer increased the effectiveness of leaner schedules of reinforcer delivery; however, it was not possible to fade the temporal schedule to one that would have been useful in practice. In addition, limitations and future research are outlined.
Date: August 2010
Creator: Lyon, Nathan Scott
Object Type: Thesis or Dissertation
System: The UNT Digital Library
A Chorus of Trees (open access)

A Chorus of Trees

This two-part thesis includes a critical preface and a collection of my poems. Using three poems-Louise Glück's "Lullaby," Bob Hicok's "Poem for My Mother's Hysterectomy," and Nick Flynn's "Memento Mori"-the critical preface examines how, in poetry, the transformation of a body negotiates trauma and triggers a conceptual shift, the creation and revision of identity, and the release of the duende's inspirational force. The collection of poetry that follows seeks to transfigure the body as a way to explore the nuanced traumas of human experience.
Date: August 2010
Creator: Lyons, Renée Kathleen
Object Type: Thesis or Dissertation
System: The UNT Digital Library
The Dichotomy of Congressional Approval (open access)

The Dichotomy of Congressional Approval

This thesis seeks to understand how political awareness affects what information one uses to indicate their approval or disapproval of Congress and its members. More concisely, do more and less aware individuals rely on the same pieces of political information to mold their opinions of Congress? The second question of concern is what role does media consumption play in informing survey respondents about Congress. Third, I consider how survey respondents use cues like the condition of the economy and presidential job performance to help formulate their opinion of Congress Finally, by applying the Congressional approval literature to incumbent level approval, I seek to advance the theory and literature on what motivates the approval of incumbents.
Date: August 2010
Creator: Moti, Danish Saleem
Object Type: Thesis or Dissertation
System: The UNT Digital Library
County Level Population Estimation Using Knowledge-Based Image Classification and Regression Models (open access)

County Level Population Estimation Using Knowledge-Based Image Classification and Regression Models

This paper presents methods and results of county-level population estimation using Landsat Thematic Mapper (TM) images of Denton County and Collin County in Texas. Landsat TM images acquired in March 2000 were classified into residential and non-residential classes using maximum likelihood classification and knowledge-based classification methods. Accuracy assessment results from the classified image produced using knowledge-based classification and traditional supervised classification (maximum likelihood classification) methods suggest that knowledge-based classification is more effective than traditional supervised classification methods. Furthermore, using randomly selected samples of census block groups, ordinary least squares (OLS) and geographically weighted regression (GWR) models were created for total population estimation. The overall accuracy of the models is over 96% at the county level. The results also suggest that underestimation normally occurs in block groups with high population density, whereas overestimation occurs in block groups with low population density.
Date: August 2010
Creator: Nepali, Anjeev
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Bad Blood: Impurity and Danger in the Early Modern Spanish Mentality (open access)

Bad Blood: Impurity and Danger in the Early Modern Spanish Mentality

The current work is an intellectual history of how blood permeated early modern Spaniards' conceptions of morality and purity. This paper examines Spanish intellectuals' references to blood in their medical, theological, demonological, and historical works. Through these excerpts, this thesis demonstrates how this language of blood played a role in buttressing the church's conception of good morals. This, in turn, will show that blood was used as a way to persecute Jews and Muslims, and ultimately define the early modern Spanish identity.
Date: August 2010
Creator: Pyle, Rhonda
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Elicitation of Protein-Protein Interactions from Biomedical Literature Using Association Rule Discovery (open access)

Elicitation of Protein-Protein Interactions from Biomedical Literature Using Association Rule Discovery

Extracting information from a stack of data is a tedious task and the scenario is no different in proteomics. Volumes of research papers are published about study of various proteins in several species, their interactions with other proteins and identification of protein(s) as possible biomarker in causing diseases. It is a challenging task for biologists to keep track of these developments manually by reading through the literatures. Several tools have been developed by computer linguists to assist identification, extraction and hypotheses generation of proteins and protein-protein interactions from biomedical publications and protein databases. However, they are confronted with the challenges of term variation, term ambiguity, access only to abstracts and inconsistencies in time-consuming manual curation of protein and protein-protein interaction repositories. This work attempts to attenuate the challenges by extracting protein-protein interactions in humans and elicit possible interactions using associative rule mining on full text, abstracts and captions from figures available from publicly available biomedical literature databases. Two such databases are used in our study: Directory of Open Access Journals (DOAJ) and PubMed Central (PMC). A corpus is built using articles based on search terms. A dataset of more than 38,000 protein-protein interactions from the Human Protein Reference Database (HPRD) …
Date: August 2010
Creator: Samuel, Jarvie John
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Effect of Resource Availability on Dyadic Fitness (open access)

Effect of Resource Availability on Dyadic Fitness

College students participating in dyads played a game designed as an analog of early hunters whose survival, as a dyad and ultimately individually, depend on rabbits they hunt. Dyadic fitness was defined as both participants being able to hunt and it was measured by the proportion of trials in a condition that both participants hunted. The effects of scarcity (alternating rich and poor conditions) on dyadic fitness were examined in two experiments. First experiment results did not show a difference in dyadic fitness as a function of the independent variable. The second experiment increased the number of hunting seasons and also the discrepancy between scarcity in rich and poor seasons. Second experiment results show that dyads start fit in rich seasons and become increasingly fit in poor seasons. External variables could not be ruled out; therefore, additional experiments still need to be carried out to clarify results.
Date: August 2010
Creator: Neves, Ana Barbara Vieira Sinay
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Do College Students with ADHD have Expressive Writing Difficulties as Do Children with ADHD? (open access)

Do College Students with ADHD have Expressive Writing Difficulties as Do Children with ADHD?

This study analyzed the expressive writing of college students. Twenty-two ADHD students and 22 controls were asked to write a story based on a picture story and a personal challenge. The texts were compared based on several qualitative and quantitative parameters. The results show that students in both groups presented similar text quality. Out of six qualitative parameters only one was statistically different between the two groups: ADHD students performed worse in adequacy, but only in the picture task. Students writings were also investigated using corpus based analysis. This analysis showed that ADHD students used less unusually frequent words in the picture story but more in the challenge task. Taken together the findings indicate no significant difference in expressive writing between ADHD and non ADHD college students. An explanation to this result is that college students with ADHD may have passed the filter of prior education.
Date: August 2010
Creator: Mantecon, Hripsime Der-Galustian
Object Type: Thesis or Dissertation
System: The UNT Digital Library
To Tell or not to Tell? An Examination of Stepparents' Communication Privacy Management (open access)

To Tell or not to Tell? An Examination of Stepparents' Communication Privacy Management

This study examined stepparents' privacy boundary management when engaging in communicative interactions with stepchildren. I utilized Petronio's communication privacy management theory to investigate stepparents' motivations of disclosing or concealing from stepchildren as well as how stepparents' gender influences such motivations. Moreover, present research also explored types of privacy dilemma within stepfamily households from stepparent perspectives. Fifteen stepfathers and 15 stepmothers received in-depth interviews about their self-disclosing and concealment experiences with stepchildren. I identified confidant dilemma and accidental dilemma in stepfamily households from stepparents' perspectives, as well as stepparents' gender differences in self-disclosing and concealing motivations. Findings also suggest that stepparents reveal and conceal from stepchildren out of same motivations: establishing good relationships, viewing stepchildren as own children, helping stepchildren with problems resulting from the divorce and viewing stepchildren as "others." The result also indicates that stepparents experienced dialectical tensions between closedness and openness during the decision of revealing or concealing from stepchildren.
Date: August 2010
Creator: Hsu, Tsai-chen
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Ultrafast Laser Sampling of a Plant Tissue and ion Conductivity Measurement for Investigation of Light Stress Generation Mechanisms (open access)

Ultrafast Laser Sampling of a Plant Tissue and ion Conductivity Measurement for Investigation of Light Stress Generation Mechanisms

In this study we applied ultra-short laser pulses on a biological sample (Arabidopsis), in order to cut it precisely in a square pattern and subsequently use it for studying stress generation mechanisms. For this purpose, we utilized femtosecond laser pulses at 100 fs pulse width and 80 MHz repetition rate. We took two processing parameters into consideration such as laser power, laser exposure time which is related to the stage speed. Therefore, we were able to find the laser optimum conditions for ablation of biological tissues. The mutant and wildtype (control) obtained from laser cutting with a size of 500 µm × 500 µm were directly transferred (in-situ with laser cutting) into a microfabricated chamber containing ~500 nanoliters deionized water for measuring ion conductivity. The ion conductivity is a signature of cell-death mechanisms caused by various stresses. A light with intensity of 100 µmol was exposed to the samples for 2 hours and 20 minutes as a source of stress. A quantitative electrical analysis with high accuracy was assured by utilizing a microchamber, which enables a measurement in nanoliter volume. We measured the impedance which is reciprocal of conductivity using a lock-in amplifier and a precise current source at frequency …
Date: August 2010
Creator: Abtahi, Seyed Ali
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Perspectives on Cultural Context: The Use of an Online Participatory Learning Environment as an Expansion of the Museum Visit (open access)

Perspectives on Cultural Context: The Use of an Online Participatory Learning Environment as an Expansion of the Museum Visit

Technology offers opportunities for museums to expand the ways in which cultural perspectives relevant to objects on display can be exchanged and understood. Multimedia content offered online in an environment with user input capabilities can encourage dialogue and enrich visitor experiences of museums. This action research project using narrative analysis was an effort to develop the use of web technology in museum education practice, with an emphasis on constructivist learning. Concepts including the visitor-centered museum and multiple narratives led the researcher to collaborate with a pre-service art teacher education classroom and a local Hindu community to create content that might better develop understandings of one museum's Hindu sculpture collection that are personal, cultural, and complex.
Date: August 2010
Creator: Sreenan, Patrick N.
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Effective and Accelerated Informative Frame Filtering in Colonoscopy Videos Using Graphic Processing Units (open access)

Effective and Accelerated Informative Frame Filtering in Colonoscopy Videos Using Graphic Processing Units

Colonoscopy is an endoscopic technique that allows a physician to inspect the mucosa of the human colon. Previous methods and software solutions to detect informative frames in a colonoscopy video (a process called informative frame filtering or IFF) have been hugely ineffective in (1) covering the proper definition of an informative frame in the broadest sense and (2) striking an optimal balance between accuracy and speed of classification in both real-time and non real-time medical procedures. In my thesis, I propose a more effective method and faster software solutions for IFF which is more effective due to the introduction of a heuristic algorithm (derived from experimental analysis of typical colon features) for classification. It contributed to a 5-10% boost in various performance metrics for IFF. The software modules are faster due to the incorporation of sophisticated parallel-processing oriented coding techniques on modern microprocessors. Two IFF modules were created, one for post-procedure and the other for real-time. Code optimizations through NVIDIA CUDA for GPU processing and/or CPU multi-threading concepts embedded in two significant microprocessor design philosophies (multi-core design and many-core design) resulted a 5-fold acceleration for the post-procedure module and a 40-fold acceleration for the real-time module. Some innovative software modules, …
Date: August 2010
Creator: Karri, Venkata Praveen
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Hepatotoxicity of Mercury to Fish (open access)

Hepatotoxicity of Mercury to Fish

Tissue samples from spotted gar (Lepisosteus oculatus) and largemouth bass (Micropterus salmoides) were collected from Caddo Lake. Gar and bass livers were subjected to histological investigation and color analysis. Liver color (as abs at 400 nm) was significantly correlated with total mercury in the liver (r2 = 0.57, p = 0.02) and muscle (r2 = 0.58, p = 0.01) of gar. Evidence of liver damage as lipofuscin and discoloration was found in both species but only correlated with liver mercury concentration in spotted gar. Inorganic mercury was the predominant form in gar livers. In order to determine the role of mercury speciation in fish liver damage, a laboratory feeding study was employed. Zebrafish (Danio rerio) were fed either a control (0.12 ± 0.002 µg Hg.g-1 dry wt), inorganic mercury (5.03 ± 0.309 µg Hg.g-1 dry wt), or methylmercury (4.11 ± 0.146 µg Hg.g-1 dry wt) diet. After 78 days of feeding, total mercury was highest in the carcass of zebrafish fed methylmercury (12.49 ± 0.369 µg Hg.g-1 dry wt), intermediate in those fed inorganic mercury (1.09 ± 0.117 µg Hg.g-1 dry wt), and lowest in fish fed the control diet (0.48 ± 0.038 µg Hg.g-1 dry wt). Total mercury was …
Date: August 2010
Creator: Barst, Benjamin Daniel
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Functional Neural Toxicity and Endocrine Responses in Mice Following Naphthalene Exposure (open access)

Functional Neural Toxicity and Endocrine Responses in Mice Following Naphthalene Exposure

Polycyclic aromatic hydrocarbons (PAHs) are a well studied and diverse class of environmental toxicants. PAHs act via the aryl hydrocarbon receptor (AhR), and studies have suggested that PAHs may elicit neurological and estrogenic effects. Doses of PAHs between 50 to 150 ppm may elicit neurotoxicity in rodent models. The present study investigated the effects of naphthalene on in vivo steroidogenesis in Swiss Webster male mice, and in vitro neural function of Balb-C/ICR mice frontal cortex neurons. These data suggest that naphthalene may not elicit steroidogenic effects at concentrations ranging from 0.2 to 25 mg/kg/day, following a 7 day subcutaneous dosing regime. In addition, naphthalene may cause functional toxicity of frontal cortex neurons at concentrations of 32 to 160 ppm naphthalene.
Date: August 2010
Creator: Colbert, Crystal
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Life without Parole for Juvenile Offenders: Questions of Legality and Adolescent Culpability (open access)

Life without Parole for Juvenile Offenders: Questions of Legality and Adolescent Culpability

Life without parole for juvenile offenders is a controversial issue across the globe. Recently, the United States stands alone as the only country in the world that allows juvenile offenders to be sentenced to life time confinement without the possibility of parole. Furthermore, the U.S. has seen an increase in juvenile waivers and blended sentences, which has resulted in harsher penalties for juvenile offenders who have committed serious and violent crimes. This analysis examines scientific evidence that shows juveniles are different from adults in terms of brain development, rational decision making abilities, and maturity levels. These findings have questioned the reasoning behind imposing adult punishment on adolescent behavior. This analysis also presents the legal arguments suggesting that juvenile life without parole is unconstitutional and violates the Eighth and Fourteenth Amendments. Arguments for and against life sentences were also presented. This study concludes with a discussion of policy implications, whether the U.S. Supreme Court should abolish juvenile life without parole sentencing practices and explores the possible future direction of juvenile sentencing in the United States.
Date: August 2010
Creator: Corrington, David L.
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Interactions of N-Acylethanolamine Metabolism and Abscisic Acid Signaling in Arabidopsis Thaliana Seedlings (open access)

Interactions of N-Acylethanolamine Metabolism and Abscisic Acid Signaling in Arabidopsis Thaliana Seedlings

N-Acylethanolamines (NAEs) are endogenous plant lipids hydrolyzed by fatty acid amide hydrolase (FAAH). When wildtype Arabidopsis thaliana seeds were germinated and grown in exogenous NAE 12:0 (35 µM and above), growth was severely reduced in a concentration dependent manner. Wildtype A. thaliana seeds sown on exogenous abscisic acid (ABA) exhibited similar growth reduction to that seen with NAE treatment. AtFAAH knockouts grew and developed similarly to WT, but AtFAAH overexpressor lines show markedly enhanced sensitivity to ABA. When low levels of NAE and ABA, which have very little effect on growth alone, were combined, there was a dramatic reduction in seedling growth in all three genotypes, indicating a synergistic interaction between ABA and NAE. Notably, this synergistic arrest of seedling growth was partially reversed in the ABA insensitive (abi) mutant abi3-1, indicating that a functional ABA signaling pathway is required for the full synergistic effect. This synergistic growth arrest results in an increased accumulation of NAEs, but no concomitant increase in ABA levels. The combined NAE and ABA treatment induced a dose-dependent increase in ABI3 transcript levels, which was inversely related to growth. The ABA responsive genes AtHVA22B and RD29B also had increased expression in both NAE and ABA treatment. …
Date: August 2010
Creator: Cotter, Matthew Q.
Object Type: Thesis or Dissertation
System: The UNT Digital Library