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Identification of Small RNAs in Desulfovibrio vulgaris Hildenborough
Desulfovibrio vulgaris is an anaerobic sulfate-reducing bacterium capable of facilitating the removal of toxic metals such as uranium from contaminated sites via reduction. As such, it is essential to understand the intricate regulatory cascades involved in how D. vulgaris and its relatives respond to stressors in such sites. One approach is the identification and analysis of small non-coding RNAs (sRNAs); molecules ranging in size from 20-200 nucleotides that predominantly affect gene regulation by binding to complementary mRNA in an anti-sense fashion and therefore provide an immediate regulatory response. To identify sRNAs in D. vulgaris, a bacterium that does not possess an annotated hfq gene, RNA was pooled from stationary and exponential phases, nitrate exposure, and biofilm conditions. The subsequent RNA was size fractionated, modified, and converted to cDNA for high throughput transcriptomic deep sequencing. A computational approach to identify sRNAs via the alignment of seven separate Desulfovibrio genomes was also performed. From the deep sequencing analysis, 2,296 reads between 20 and 250 nt were identified with expression above genome background. Analysis of those reads limited the number of candidates to ~;;87 intergenic, while ~;;140 appeared to be antisense to annotated open reading frames (ORFs). Further BLAST analysis of the intergenic …
Date:
May 17, 2010
Creator:
Burns, Andrew; Joachimiak, Marcin; Deutschbauer, Adam; Arkin, Adam & Bender, Kelly
Object Type:
Poster
System:
The UNT Digital Library
The Altus Times (Altus, Okla.), Vol. 112, No. 30, Ed. 1 Sunday, January 17, 2010
Daily newspaper from Altus, Oklahoma that includes local, state, and national news along with advertising.
Date:
January 17, 2010
Creator:
Bush, Michael
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
The Altus Times (Altus, Okla.), Vol. 112, No. 52, Ed. 1 Wednesday, February 17, 2010
Daily newspaper from Altus, Oklahoma that includes local, state, and national news along with advertising.
Date:
February 17, 2010
Creator:
Bush, Michael
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
The Altus Times (Altus, Okla.), Vol. 112, No. 72, Ed. 1 Wednesday, March 17, 2010
Daily newspaper from Altus, Oklahoma that includes local, state, and national news along with advertising.
Date:
March 17, 2010
Creator:
Bush, Michael
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
The Altus Times (Altus, Okla.), Vol. 112, No. 138, Ed. 1 Thursday, June 17, 2010
Daily newspaper from Altus, Oklahoma that includes local, state, and national news along with advertising.
Date:
June 17, 2010
Creator:
Bush, Michael
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
The Altus Times (Altus, Okla.), Vol. 112, No. 181, Ed. 1 Tuesday, August 17, 2010
Daily newspaper from Altus, Oklahoma that includes local, state, and national news along with advertising.
Date:
August 17, 2010
Creator:
Bush, Michael
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
The Altus Times (Altus, Okla.), Vol. 112, No. 204, Ed. 1 Friday, September 17, 2010
Daily newspaper from Altus, Oklahoma that includes local, state, and national news along with advertising.
Date:
September 17, 2010
Creator:
Bush, Michael
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
The Altus Times (Altus, Okla.), Vol. 112, No. 225, Ed. 1 Sunday, October 17, 2010
Daily newspaper from Altus, Oklahoma that includes local, state, and national news along with advertising.
Date:
October 17, 2010
Creator:
Bush, Michael
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
The Altus Times (Altus, Okla.), Vol. 112, No. 247, Ed. 1 Wednesday, November 17, 2010
Daily newspaper from Altus, Oklahoma that includes local, state, and national news along with advertising.
Date:
November 17, 2010
Creator:
Bush, Michael
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
The Altus Times (Altus, Okla.), Vol. 112, No. 269, Ed. 1 Friday, December 17, 2010
Daily newspaper from Altus, Oklahoma that includes local, state, and national news along with advertising.
Date:
December 17, 2010
Creator:
Bush, Michael
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
High-Power Solid-State Lasers from a Laser Glass Perspective
Advances in laser glass compositions and manufacturing have enabled a new class of high-energy/high-power (HEHP), petawatt (PW) and high-average-power (HAP) laser systems that are being used for fusion energy ignition demonstration, fundamental physics research and materials processing, respectively. The requirements for these three laser systems are different necessitating different glasses or groups of glasses. The manufacturing technology is now mature for melting, annealing, fabricating and finishing of laser glasses for all three applications. The laser glass properties of major importance for HEHP, PW and HAP applications are briefly reviewed and the compositions and properties of the most widely used commercial laser glasses summarized. Proposed advances in these three laser systems will require new glasses and new melting methods which are briefly discussed. The challenges presented by these laser systems will likely dominate the field of laser glass development over the next several decades.
Date:
December 17, 2010
Creator:
Campbell, J H; Hayden, J S & Marker, A J
Object Type:
Article
System:
The UNT Digital Library
South Texas Catholic (Corpus Christi, Tex.), Vol. 45, No. 13, Ed. 1 Friday, September 17, 2010
Semi-monthly newspaper from Corpus Christi, Texas published by the Diocese of Corpus Christi that includes news of interest to Diocese members along with advertising.
Date:
September 17, 2010
Creator:
Cardenas, Alfredo E.
Object Type:
Newspaper
System:
The Portal to Texas History
South Texas Catholic (Corpus Christi, Tex.), Vol. 45, No. 16, Ed. 1 Friday, December 17, 2010
Semi-monthly newspaper from Corpus Christi, Texas published by the Diocese of Corpus Christi that includes news of interest to Diocese members along with advertising.
Date:
December 17, 2010
Creator:
Cardenas, Alfredo E.
Object Type:
Newspaper
System:
The Portal to Texas History
Microbial community changes during sustained Cr(VI) reduction at the 100H site in Hanford, WA
Hexavalent Chromium is a widespread contaminant found in soil, sediment, and groundwater. In order to stimulate microbially-mediated reduction of Cr(VI), a poly-lactate compound (HRC) was injected into the Chromium-contaminated aquifer at the Hanford (WA) 100H site in 2004. Cr(VI) concentrations rapidly declined to below the detection limit and remained so for more than three years after injection. Based on the results of the bacterial community composition using high-density DNA 16S rRNA gene microarrays, we observed the community to transition through denitrifying, ironreducing and sulfate-reducing populations. As a result, we specifically focused isolation efforts on three bacterial species that were significant components of the community. Positive enrichments in defined anaerobic media resulted in the isolation of an iron-reducing Geobacter metallireducens-like isolate, a sulfate-reducing Desulfovibrio vukgaris-like strain and a nitrate-reducing Pseudomonas stutzeri-like isolate among several others. All of these isolates were capable of reducing Cr(VI) anoxically and have been submitted for genome sequencing to JGI. To further characterize the microbial, and geochemical mechanisms associated with in situ Cr(VI) reduction at the site, additional HRC was injected in 2008. The goal was to restimulate the indigenous microbial community and to regenerate the reducing conditions necessary for continued Cr(VI) bio-immobilization in the groundwater. Analysis …
Date:
May 17, 2010
Creator:
Chakraborty, Romy; Brodie, Eoin L; Faybishenko, Boris; Piceno, Yvette M; Tom, Lauren; Choudhuri, Swati et al.
Object Type:
Report
System:
The UNT Digital Library
Master's Recital: 2010-04-17 - Daniel Chapa, euphonium
Access:
Use of this item is restricted to the UNT Community
Recital presented at the UNT College of Music Recital Hall in partial fulfillment of the Master of Music (MM) degree.
Date:
April 17, 2010
Creator:
Chapa, Danny
Object Type:
Sound
System:
The UNT Digital Library
Surface Phenomena and Parameters of Crystal Growth: Simple Basics
None
Date:
May 17, 2010
Creator:
Chernov, A A
Object Type:
Article
System:
The UNT Digital Library
Review of Quantitative Software Reliability Methods
The current U.S. Nuclear Regulatory Commission (NRC) licensing process for digital systems rests on deterministic engineering criteria. In its 1995 probabilistic risk assessment (PRA) policy statement, the Commission encouraged the use of PRA technology in all regulatory matters to the extent supported by the state-of-the-art in PRA methods and data. Although many activities have been completed in the area of risk-informed regulation, the risk-informed analysis process for digital systems has not yet been satisfactorily developed. Since digital instrumentation and control (I&C) systems are expected to play an increasingly important role in nuclear power plant (NPP) safety, the NRC established a digital system research plan that defines a coherent set of research programs to support its regulatory needs. One of the research programs included in the NRC's digital system research plan addresses risk assessment methods and data for digital systems. Digital I&C systems have some unique characteristics, such as using software, and may have different failure causes and/or modes than analog I&C systems; hence, their incorporation into NPP PRAs entails special challenges. The objective of the NRC's digital system risk research is to identify and develop methods, analytical tools, and regulatory guidance for (1) including models of digital systems into NPP …
Date:
September 17, 2010
Creator:
Chu, T. L.; Yue, M.; Martinez-Guridi, M. & Lehner, J.
Object Type:
Report
System:
The UNT Digital Library
Doctoral Lecture Recital: 2010-05-17 – Jennifer Ciobanu, soprano
Recital presented at the UNT College of Music Recital Hall in partial fulfillment of the Doctor of Musical Arts (DMA) degree.
Date:
May 17, 2010
Creator:
Ciobanu, Jennifer
Object Type:
Sound
System:
The UNT Digital Library
Water use in the development and operation of geothermal power plants.
Geothermal energy is increasingly recognized for its potential to reduce carbon emissions and U.S. dependence on foreign oil. Energy and environmental analyses are critical to developing a robust set of geothermal energy technologies. This report summarizes what is currently known about the life cycle water requirements of geothermal electric power-generating systems and the water quality of geothermal waters. It is part of a larger effort to compare the life cycle impacts of large-scale geothermal electricity generation with other power generation technologies. The results of the life cycle analysis are summarized in a companion report, Life Cycle Analysis Results of Geothermal Systems in Comparison to Other Power Systems. This report is divided into six chapters. Chapter 1 gives the background of the project and its purpose, which is to inform power plant design and operations. Chapter 2 summarizes the geothermal electricity generation technologies evaluated in this study, which include conventional hydrothermal flash and binary systems, as well as enhanced geothermal systems (EGS) that rely on engineering a productive reservoir where heat exists but water availability or permeability may be limited. Chapter 3 describes the methods and approach to this work and identifies the four power plant scenarios evaluated: a 20-MW EGS …
Date:
September 17, 2010
Creator:
Clark, C. E.; Harto, C. B.; Sullivan, J. L.; Wang, M. Q. (Energy Systems) & EVS), (
Object Type:
Report
System:
The UNT Digital Library
The Baytown Sun (Baytown, Tex.), Vol. 90, No. 34, Ed. 1 Wednesday, February 17, 2010
Daily newspaper from Baytown, Texas that includes local, state, and national news along with advertising.
Date:
February 17, 2010
Creator:
Clements, Clifford E.
Object Type:
Newspaper
System:
The Portal to Texas History
Tungsten transport in the NSTX tokamak
None
Date:
May 17, 2010
Creator:
Clementson, J; Beiersdorfer, P; Roquemore, A L; Skinner, C H; Mansfield, D K; Hartzfeld, K et al.
Object Type:
Article
System:
The UNT Digital Library
A Comparison of Three Voting Methods for Bagging with the MLEM2 Algorithm
This paper presents results of experiments on some data sets using bagging on the MLEM2 rule induction algorithm. Three different methods of ensemble voting, based on support (a non-democratic voting in which ensembles vote with their strengths), strength only (an ensemble with the largest strength decides to which concept a case belongs) and democratic voting (each ensemble has at most one vote) were used. Our conclusions are that though in most cases democratic voting was the best, it is not significantly better than voting based on support. The strength voting was the worst voting method.
Date:
March 17, 2010
Creator:
Cohagan, Clinton; Grzymala-Busse, Jerzy W. & Hippe, Zdzislaw S.
Object Type:
Article
System:
The UNT Digital Library
Development of an Extreme Environment Materials Research Facility at Princeton
The need for a fundamental understanding of material response to a neutron and/or high heat flux environment can yield development of improved materials and operations with existing materials. Such understanding has numerous applications in fields such as nuclear power (for the current fleet and future fission and fusion reactors), aerospace, and other research fields (e.g., high-intensity proton accelerator facilities for high energy physics research). A proposal has been advanced to develop a facility for testing various materials under extreme heat and neutron exposure conditions at Princeton. The Extreme Environment Materials Research Facility comprises an environmentally controlled chamber (48 m^3) capable of high vacuum conditions, with extreme flux beams and probe beams accessing a central, large volume target. The facility will have the capability to expose large surface areas (1 m^2) to 14 MeV neutrons at a fluence in excess of 10^13 n/s. Depending on the operating mode. Additionally beam line power on the order of 15-75 MW/m2 for durations of 1-15 seconds are planned... The multi-second duration of exposure can be repeated every 2-10 minutes for periods of 10-12 hours. The facility will be housed in the test cell that held the Tokamak Fusion Test Reactor (TFTR), which has the …
Date:
November 17, 2010
Creator:
Cohen, A. B.; Tully, C. G.; Austin, R.; Calaprice, F.; McDonald, K.; Ascione, G. et al.
Object Type:
Article
System:
The UNT Digital Library
MEASUREMENT OF THE ANISOTROPY OF COSMIC RAY ARRIVAL DIRECTIONS WITH ICECUBE
We report the first observation of an anisotropy in the arrival direction of cosmic rays with energies in the multi TeV region in the Southern sky using data from the IceCube detector. Between June 2007 and March 2008, the partially-deployed IceCube detector was operated in a configuration with 1320 digital optical sensors distributed over 22 strings at depths between 1450 and 2450 meters inside the Antarctic ice. IceCube is a neutrino detector, but the data are dominated by a large background of cosmic ray muons. Therefore, the background data are suitable for high-statistics studies of cosmic rays in the Southern sky. The data include 4.3 billion muons produced by downgoing cosmic ray interactions in the atmosphere; these events were reconstructed with a median angular resolution of 3 degrees and a median energy of ~;; 20 TeV. Their arrival direction distribution exhibits an anisotropy in right ascension with a first harmonic amplitude of (6.4 +- 0.2 stat. +- 0.8 syst.) x 10-4.
Date:
May 17, 2010
Creator:
Collaboration, IceCube & Abbasi, R.
Object Type:
Article
System:
The UNT Digital Library