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Vehicle to Grid Demonstration Project (open access)

Vehicle to Grid Demonstration Project

This report summarizes the activities and accomplishments of a two-year DOE-funded project on Grid-Integrated Vehicles (GIV) with vehicle to grid power (V2G). The project included several research and development components: an analysis of US driving patterns; an analysis of the market for EVs and V2G-capable EVs; development and testing of GIV components (in-car and in-EVSE); interconnect law and policy; and development and filing of patents. In addition, development activities included GIV manufacturing and licensing of technologies developed under this grant. Also, five vehicles were built and deployed, four for the fleet of the State of Delaware, plus one for the University of Delaware fleet.
Date: December 31, 2010
Creator: Kempton, Willett; Gardner, Meryl; Hidrue, Michael; Kamilev, Fouad; Kamboj, Sachin; Lilley, Jon et al.
Object Type: Report
System: The UNT Digital Library
Prototype Power and Communications System for EeV Cosmic Rays Studies (open access)

Prototype Power and Communications System for EeV Cosmic Rays Studies

An analysis of improving the power output of small wind turbines by adding a venturi housing was done. Including the effects of back pressure developed at the input to the housing lowers the efficiency gain from a factor of 5 to a factor of 2 for a turbine blade radius of 24 inches. The gain is small enough that only large systems could profit from the application.
Date: August 31, 2010
Creator: Russ, James S.
Object Type: Text
System: The UNT Digital Library
100 LPW 800 Lm Warm White LED (open access)

100 LPW 800 Lm Warm White LED

An illumination grade warm white (WW) LED, having correlated color temperature (CCT) between 2800 K and 3500K and capable of producing 800 lm output at 100 lm/W, has been developed in this program. The high power WW LED is an ideal source for use as replacement for incandescent, and Halogen reflector and general purpose lamps of similar lumen value. Over the two year period, we have made following accomplishments: developed a high power warm white LED product and made over 50% improvements in light output and efficacy. The new high power WW LED product is a die on ceramic surface mountable LED package. It has four 1x1 mm{sup 2} InGaN pump dice flip chip attached to a ceramic submount in 2x2 array, covered by warm white phosphor ceramic platelets called Lumiramic™ and an overmolded silicone lens encapsulating the LED array. The performance goal was achieved through breakthroughs in following key areas: (1) High efficiency pump LED development through pump LED active region design and epi growth quality improvement (funded by internal programs). (2) Increase in injection efficiency (IE) represented by reduction in forward voltage (V{sub f}) through the improvement of the silver-based p-contact and a reduction in spreading resistance. The …
Date: October 31, 2010
Creator: Sun, Decai
Object Type: Report
System: The UNT Digital Library
ADVANCED GASIFICATION MERCURY/TRACE METAL CONTROL WITH MONOLITH TRAPS (open access)

ADVANCED GASIFICATION MERCURY/TRACE METAL CONTROL WITH MONOLITH TRAPS

Two Corning monoliths and a non-carbon-based material have been identified as potential additives for mercury capture in syngas at temperatures above 400°F and pressure of 600 psig. A new Corning monolith formulation, GR-F1-2189, described as an active sample appeared to be the best monolith tested to date. The Corning SR Liquid monolith concept continues to be a strong candidate for mercury capture. Both monolith types allowed mercury reduction to below 5-μg/m3 (~5 ppb), a current U.S. Department of Energy (DOE) goal for trace metal control. Preparation methods for formulating the SR Liquid monolith impacted the ability of the monolith to capture mercury. The Energy & Environmental Research Center (EERC)-prepared Noncarbon Sorbents 1 and 2 appeared to offer potential for sustained and significant reduction of mercury concentration in the simulated fuel gas. The Noncarbon Sorbent 1 allowed sustained mercury reduction to below 5-μg/m3 (~5 ppb). The non-carbon-based sorbent appeared to offer the potential for regeneration, that is, desorption of mercury by temperature swing (using nitrogen and steam at temperatures above where adsorption takes place). A Corning cordierite monolith treated with a Group IB metal offered limited potential as a mercury sorbent. However, a Corning carbon-based monolith containing prereduced metallic species similar …
Date: July 31, 2010
Creator: Musich, Mark A.; Swanson, Michael L.; Dunham, Grant E. & Stanislowski, Joshua J.
Object Type: Report
System: The UNT Digital Library
Hydroacoustic Evaluation of Overwintering Summer Steelhead Fallback and Kelt Passage at The Dalles Dam, 2009-2010 (open access)

Hydroacoustic Evaluation of Overwintering Summer Steelhead Fallback and Kelt Passage at The Dalles Dam, 2009-2010

This report presents the results of an evaluation of overwintering summer steelhead (Oncorhynchus mykiss) fallback and early out-migrating steelhead kelts downstream passage at The Dalles Dam (TDA) sluiceway and turbines during fall/winter 2009 through early spring 2010. The study was conducted by the Pacific Northwest National Laboratory (PNNL) for the U.S. Army Corps of Engineers, Portland District (USACE). The goal of this study was to characterize adult steelhead spatial and temporal distributions and passage rates at the sluiceway and turbines for fisheries managers and engineers to use in decision-making relative to sluiceway operations. The study was from November 1, 2009 to April 10, 2010. The study was divided into three study periods: Period 1, November 1 - December 15, 2009 for a fall/winter sluiceway and turbine study; Period 2, December 16, 2009 - February 28, 2010 for a turbine only study; Period 3, March 1 - April 10, 2010 for a spring sluiceway and turbine study. Sluiceway operations were scheduled to begin on March 1 for this study; however, because of an oil spill cleanup near the sluice outfall, sluiceway operations were delayed until March 8, 2010, therefore the spring study period did not commence until March 8. The study …
Date: July 31, 2010
Creator: Khan, Fenton; Johnson, Gary E. & Weiland, Mark A.
Object Type: Report
System: The UNT Digital Library
[Minutes of the TXSSAR Board of Managers Meeting: July 31-August 1, 2010] (open access)

[Minutes of the TXSSAR Board of Managers Meeting: July 31-August 1, 2010]

Minutes of the Texas Society of the Sons of the American Revolution (TXSSAR) Board of Managers meeting covering the general sessions, held July 31-August 1, 2010 at the Holiday Inn, in Austin, Texas. It includes information about the committees and business covered by the attending members.
Date: 2010-07-31/2010-08-01
Creator: unknown
Object Type: Text
System: The UNT Digital Library
Yoakum Herald-Times (Yoakum, Tex.), Vol. 118, No. 13, Ed. 1 Wednesday, March 31, 2010 (open access)

Yoakum Herald-Times (Yoakum, Tex.), Vol. 118, No. 13, Ed. 1 Wednesday, March 31, 2010

Weekly newspaper from Yoakum, Texas that includes local, state, and national news along with advertising.
Date: March 31, 2010
Creator: McCracken, Michael S.
Object Type: Newspaper
System: The Portal to Texas History
The Clifton Record (Clifton, Tex.), Vol. 116, No. 12, Ed. 1 Wednesday, March 31, 2010 (open access)

The Clifton Record (Clifton, Tex.), Vol. 116, No. 12, Ed. 1 Wednesday, March 31, 2010

Weekly newspaper from Clifton, Texas that includes local, state, and national news along with advertising.
Date: March 31, 2010
Creator: Henry, Mark
Object Type: Newspaper
System: The Portal to Texas History
3-D Mapping Technologies for High Level Waste Tanks (open access)

3-D Mapping Technologies for High Level Waste Tanks

This research investigated four techniques that could be applicable for mapping of solids remaining in radioactive waste tanks at the Savannah River Site: stereo vision, LIDAR, flash LIDAR, and Structure from Motion (SfM). Stereo vision is the least appropriate technique for the solids mapping application. Although the equipment cost is low and repackaging would be fairly simple, the algorithms to create a 3D image from stereo vision would require significant further development and may not even be applicable since stereo vision works by finding disparity in feature point locations from the images taken by the cameras. When minimal variation in visual texture exists for an area of interest, it becomes difficult for the software to detect correspondences for that object. SfM appears to be appropriate for solids mapping in waste tanks. However, equipment development would be required for positioning and movement of the camera in the tank space to enable capturing a sequence of images of the scene. Since SfM requires the identification of distinctive features and associates those features to their corresponding instantiations in the other image frames, mockup testing would be required to determine the applicability of SfM technology for mapping of waste in tanks. There may be …
Date: August 31, 2010
Creator: Marzolf, A. & Folsom, M.
Object Type: Article
System: The UNT Digital Library
Reactive Transport and Coupled THM Processes in Engineering Barrier Systems (EBS) (open access)

Reactive Transport and Coupled THM Processes in Engineering Barrier Systems (EBS)

Geological repositories for disposal of high-level nuclear wastes generally rely on a multi-barrier system to isolate radioactive wastes from the biosphere. The multi-barrier system typically consists of a natural barrier system, including repository host rock and its surrounding subsurface environment, and an engineering barrier system (EBS). EBS represents the man-made, engineered materials placed within a repository, including the waste form, waste canisters, buffer materials, backfill and seals (OECD, 2003). EBS plays a significant role in the containment and long-term retardation of radionuclide release. EBS is involved in complex thermal, hydrogeological, mechanical, chemical and biological processes, such as heat release due to radionuclide decay, multiphase flow (including gas release due to canister corrosion), swelling of buffer materials, radionuclide diffusive transport, waste dissolution and chemical reactions. All these processes are related to each other. An in-depth understanding of these coupled processes is critical for the performance assessment (PA) for EBS and the entire repository. Within the EBS group of Used Fuel Disposition (UFD) Campaign, LBNL is currently focused on (1) thermal-hydraulic-mechanical-chemical (THMC) processes in buffer materials (bentonite) and (2) diffusive transport in EBS associated with clay host rock, with a long-term goal to develop a full understanding of (and needed modeling capabilities …
Date: August 31, 2010
Creator: Steefel, Carl; Rutqvist, Jonny; Tsang, Chin-Fu; Liu, Hui-Hai; Sonnenthal, Eric; Houseworth, Jim et al.
Object Type: Report
System: The UNT Digital Library
Black Silicon Enhanced Thin Film Silicon Photovoltaic Devices (open access)

Black Silicon Enhanced Thin Film Silicon Photovoltaic Devices

SiOnyx has developed an enhanced thin film silicon photovoltaic device with improved efficiency. Thin film silicon solar cells suffer from low material absorption characteristics resulting in poor cell efficiencies. SiOnyx’s approach leverages Black Silicon, an advanced material fabricated using ultrafast lasers. The laser treated films show dramatic enhancement in optical absorption with measured values in excess of 90% in the visible spectrum and well over 50% in the near infrared spectrum. Thin film Black Silicon solar cells demonstrate 25% higher current generation with almost no impact on open circuit voltage as compared with representative control samples. The initial prototypes demonstrated an improvement of nearly 2 percentage points in the suns Voc efficiency measurement. In addition we validated the capability to scale this processing technology to the throughputs (< 5 min/m2) required for volume production using state of the art commercially available high power industrial lasers. With these results we clearly demonstrate feasibility for the enhancement of thin film solar cells with this laser processing technique.
Date: July 31, 2010
Creator: Pralle, Martin U. & Carey, James E.
Object Type: Report
System: The UNT Digital Library
REFERENCE CASES FOR USE IN THE CEMENTITOUS PARTNERSHIP PROJECT (open access)

REFERENCE CASES FOR USE IN THE CEMENTITOUS PARTNERSHIP PROJECT

The Cementitious Barriers Partnership Project (CBP) is a multi-disciplinary, multi-institution cross cutting collaborative effort supported by the US Department of Energy (DOE) to develop a reasonable and credible set of tools to improve understanding and prediction of the structural, hydraulic and chemical performance of cementitious barriers used in nuclear applications. The period of performance is &gt;100 years for operating facilities and &gt; 1000 years for waste management. The CBP has defined a set of reference cases to provide the following functions: (i) a common set of system configurations to illustrate the methods and tools developed by the CBP, (ii) a common basis for evaluating methodology for uncertainty characterization, (iii) a common set of cases to develop a complete set of parameter and changes in parameters as a function of time and changing conditions, (iv) a basis for experiments and model validation, and (v) a basis for improving conceptual models and reducing model uncertainties. These reference cases include the following two reference disposal units and a reference storage unit: (i) a cementitious low activity waste form in a reinforced concrete disposal vault, (ii) a concrete vault containing a steel high-level waste tank filled with grout (closed high-level waste tank), and (iii) …
Date: August 31, 2010
Creator: Langton, C.; Kosson, D. & Garrabrants, A.
Object Type: Report
System: The UNT Digital Library
Systematic Modification of Monolignol Pathway Gene Expression for Improved Lignocellulose Utilization (open access)

Systematic Modification of Monolignol Pathway Gene Expression for Improved Lignocellulose Utilization

Abstracts and brief summaries are provided for six published articles and seventeen poster and oral presentations at scientific meetings.
Date: August 31, 2010
Creator: Dixon, Richard A.
Object Type: Report
System: The UNT Digital Library
A Study of a Diesel Engine Based Micro-CHP System (open access)

A Study of a Diesel Engine Based Micro-CHP System

This project, funded by New York State Energy Research and Development Agency (NYSERDA), investigated the potential for an oil-fired combined heat and power system (micro-CHP system) for potential use in residences that use oil to heat their homes. Obviously, this requires the power source to be one that uses heating oil (diesel). The work consisted of an experimental study using a diesel engine and an analytical study that examined potential energy savings and benefits of micro-CHP systems for 'typical' locations in New York State. A search for a small diesel engine disclosed that no such engines were manufactured in the U.S. A single cylinder engine manufactured in Germany driving an electric generator was purchased for the experimental work. The engine was tested using on-road diesel fuel (15 ppm sulfur), and biodiesel blends. One of the main objectives was to demonstrate the possibility of operation in the so-called HCCI (Homogeneous Charge Compression Ignition) mode. The HCCI mode of operation of engines is being explored as a way to reduce the emission of smoke, and NOx significantly without exhaust treatment. This is being done primarily in the context of engines used in transportation applications. However, it is felt that in a micro-CHP …
Date: August 31, 2010
Creator: Krishna, C. R.; Andrews, J.; Tutu, N. & Butcher, T.
Object Type: Report
System: The UNT Digital Library
Tcap Hydrogen Isotope Separation Using Palladium and Inverse Columns (open access)

Tcap Hydrogen Isotope Separation Using Palladium and Inverse Columns

The Thermal Cycling Absorption Process (TCAP) was further studied with a new configuration. Previous configuration used a palladium packed column and a plug flow reverser (PFR). This new configuration uses an inverse column to replace the PFR. The goal was to further improve performance. Both configurations were experimentally tested. The results showed that the new configuration increased the throughput by a factor of more than 2.
Date: August 31, 2010
Creator: Heung, L.; Sessions, H. & Xiao, S.
Object Type: Article
System: The UNT Digital Library
REMOVAL OF CESIUM FROM SAVANNAH RIVER SITE WASTE WITH SPHERICAL RESORCINOL FORMALDEHYDE ION EXCHANGE RESIN EXPERIMENTAL TESTS (open access)

REMOVAL OF CESIUM FROM SAVANNAH RIVER SITE WASTE WITH SPHERICAL RESORCINOL FORMALDEHYDE ION EXCHANGE RESIN EXPERIMENTAL TESTS

A principal goal at the Savannah River Site (SRS) is to safely dispose of the large volume of liquid nuclear waste held in many storage tanks. In-tank ion exchange (IX) columns are being considered for cesium removal. The spherical form of resorcinol formaldehyde ion exchange resin (sRF) is being evaluated for decontamination of dissolved saltcake waste at SRS, which is generally lower in potassium and organic components than Hanford waste. The sRF performance with SRS waste was evaluated in two phases: resin batch contacts and IX column testing with both simulated and actual dissolved salt waste. The tests, equipment, and results are discussed.
Date: March 31, 2010
Creator: Duignan, M. & Nash, C.
Object Type: Article
System: The UNT Digital Library
Evaluation of Fuel Consumption Potential of Medium and Heavy Duty Vehicles Through Modeling and Simulation. (open access)

Evaluation of Fuel Consumption Potential of Medium and Heavy Duty Vehicles Through Modeling and Simulation.

The main objective of this report is to provide quantitative data to support the Committee in its task of establishing a report to support rulemaking on medium- and heavy-duty fuel efficiency improvement. In particular, it is of paramount importance for the Committee to base or illustrate their conclusions on established models and actual state-of-the art data. The simulations studies presented in the report have been defined and requested by the members of the National Academy committee to provide quantitative inputs to support their recommendations. As such, various technologies and usage scenarios were considered for several applications. One of the objective is to provide the results along with their associated assumptions (both vehicle and drive cycles), information generally missing from public discussions on literature search. Finally, the advantages and limitations of using simulation will be summarized. The study addresses several of the committee tasks, including: (1) Discussion of the implication of metric selection; (2) Assessing the impact of existing technologies on fuel consumption through energy balance analysis (both steady-state and standard cycles) as well as real world drive cycles; and (3) Impact of future technologies, both individually and collectively.
Date: March 31, 2010
Creator: Delorme, A.; Karbowski, D.; Sharer, P. & Systems, Energy
Object Type: Report
System: The UNT Digital Library
A Soft X-ray Spectrometer using a Highly Dispersive Multilayer Grating (open access)

A Soft X-ray Spectrometer using a Highly Dispersive Multilayer Grating

There is a need for higher resolution spectrometers as a tool for inelastic x-ray scattering. Currently, resolving power around R = 10,000 is advertised. Measured RIXS spectra are often limited by this instrumental resolution and higher resolution spectrometers using conventional gratings would be prohibitively large. We are engaged in a development program to build blazed multilayer grating structures for diffracting soft x-rays in high order. This leads to spectrometers with dispersion much higher than is possible using metal coated-gratings. The higher dispersion then provides higher resolution and the multilayer gratings are capable of operating away from grazing incidence as required. A spectrometer design is presented with a total length 3.8m and capable of 10{sup 5} resolving power.
Date: January 31, 2010
Creator: Warwick, Tony; Padmore, Howard; Voronov, Dmitriy & Yashchuk, Valeriy
Object Type: Article
System: The UNT Digital Library
Enabling Advanced Modeling and Simulations for Fuel-Flexible Combustors (open access)

Enabling Advanced Modeling and Simulations for Fuel-Flexible Combustors

The overall goal of the present project is to enable advanced modeling and simulations for the design and optimization of fuel-flexible turbine combustors. For this purpose we use a high fidelity, extensively-tested large-eddy simulation (LES) code and state-of-the-art models for premixed/partially-premixed turbulent combustion developed in the PI's group. In the frame of the present project, these techniques are applied, assessed, and improved for hydrogen enriched premixed and partially premixed gas-turbine combustion. Our innovative approaches include a completely consistent description of flame propagation; a coupled progress variable/level set method to resolve the detailed flame structure, and incorporation of thermal-diffusion (non-unity Lewis number) effects. In addition, we have developed a general flamelet-type transformation holding in the limits of both non-premixed and premixed burning. As a result, a model for partially premixed combustion has been derived. The coupled progress variable/level method and the general flamelet transformation were validated by LES of a lean-premixed low-swirl burner that has been studied experimentally at Lawrence Berkeley National Laboratory. The model is extended to include the non-unity Lewis number effects, which play a critical role in fuel-flexible combustor with high hydrogen content fuel. More specifically, a two-scalar model for lean hydrogen and hydrogen-enriched combustion is developed and …
Date: May 31, 2010
Creator: Pitsch, Heinz
Object Type: Report
System: The UNT Digital Library
Application for Permit to Operate a Class II Solid Waste Disposal Site at the Nevada Test Site - U10c Disposal Site (open access)

Application for Permit to Operate a Class II Solid Waste Disposal Site at the Nevada Test Site - U10c Disposal Site

The Nevada Test Site (NTS) is located approximately 105 km (65 mi) northwest of Las Vegas, Nevada. National Nuclear Security Administration Nevada Site Office (NNSA/NSO) is the federal lands management authority for the NTS and National Security Technologies LLC (NSTec) is the Management and Operations contractor. Access on and off the NTS is tightly controlled, restricted, and guarded on a 24-hour basis. The NTS is posted with signs along its entire perimeter. NSTec is the operator of all solid waste disposal sites on the NTS. The site will be used for the disposal of refuse, rubbish, garbage, sewage sludge, pathological waste, Asbestos-Containing Material (ACM), industrial solid waste, hydrocarbon-burdened soil, hydrocarbon-burdened demolition and construction waste, and other inert waste (hereafter called permissible waste). Waste containing free liquids or regulated under Subtitle C of the Resource Conservation and Recovery Act (RCRA) will not be accepted for disposal at the site. Waste regulated under the Toxic Substance Control Act (TSCA), excluding Polychlorinated Biphenyl [PCB], Bulk Product Waste (see Section 6.2.5) and ACM (see Section 6.2.2.2) will not be accepted for disposal at the site. The disposal site will be used as the sole depository of permissible waste which is: (1) Generated by entities …
Date: March 31, 2010
Creator: Programs, NSTec Environmental
Object Type: Report
System: The UNT Digital Library
Modeling Coupled Processes in Clay Formations for Radioactive Waste Disposal (open access)

Modeling Coupled Processes in Clay Formations for Radioactive Waste Disposal

As a result of the termination of the Yucca Mountain Project, the United States Department of Energy (DOE) has started to explore various alternative avenues for the disposition of used nuclear fuel and nuclear waste. The overall scope of the investigation includes temporary storage, transportation issues, permanent disposal, various nuclear fuel types, processing alternatives, and resulting waste streams. Although geologic disposal is not the only alternative, it is still the leading candidate for permanent disposal. The realm of geologic disposal also offers a range of geologic environments that may be considered, among those clay shale formations. Figure 1-1 presents the distribution of clay/shale formations within the USA. Clay rock/shale has been considered as potential host rock for geological disposal of high-level nuclear waste throughout the world, because of its low permeability, low diffusion coefficient, high retention capacity for radionuclides, and capability to self-seal fractures induced by tunnel excavation. For example, Callovo-Oxfordian argillites at the Bure site, France (Fouche et al., 2004), Toarcian argillites at the Tournemire site, France (Patriarche et al., 2004), Opalinus clay at the Mont Terri site, Switzerland (Meier et al., 2000), and Boom clay at Mol site, Belgium (Barnichon et al., 2005) have all been under intensive …
Date: August 31, 2010
Creator: Liu, Hui-Hai; Rutqvist, Jonny; Zheng, Liange; Sonnenthal, Eric; Houseworth, Jim & Birkholzer, Jens
Object Type: Report
System: The UNT Digital Library
Jackson Park Hospital Green Building Medical Center (open access)

Jackson Park Hospital Green Building Medical Center

Jackson Park Hospital completed the construction of a new Medical Office Building on its campus this spring. The new building construction has adopted the City of Chicago's recent focus on protecting the environment, and conserving energy and resources, with the introduction of green building codes. Located in a poor, inner city neighborhood on the South side of Chicago, Jackson Park Hospital has chosen green building strategies to help make the area a better place to live and work. The new green building houses the hospital's Family Medicine Residency Program and Specialty Medical Offices. The residency program has been vital in attracting new, young physicians to this medically underserved area. The new outpatient center will also help to allure needed medical providers to the community. The facility also has areas designated to women's health and community education. The Community Education Conference Room will provide learning opportunities to area residents. Emphasis will be placed on conserving resources and protecting our environment, as well as providing information on healthcare access and preventive medicine. The new Medical Office Building was constructed with numerous energy saving features. The exterior cladding of the building is an innovative, locally-manufactured precast concrete panel system with integral insulation that …
Date: March 31, 2010
Creator: Dorsey, William & Vasquez, Nelson
Object Type: Report
System: The UNT Digital Library
Advances in coupled safety modeling using systems analysis and high-fidelity methods. (open access)

Advances in coupled safety modeling using systems analysis and high-fidelity methods.

The potential for a sodium-cooled fast reactor to survive severe accident initiators with no damage has been demonstrated through whole-plant testing in EBR-II and FFTF. Analysis of the observed natural protective mechanisms suggests that they would be characteristic of a broad range of sodium-cooled fast reactors utilizing metal fuel. However, in order to demonstrate the degree to which new, advanced sodium-cooled fast reactor designs will possess these desired safety features, accurate, high-fidelity, whole-plant dynamics safety simulations will be required. One of the objectives of the advanced safety-modeling component of the Reactor IPSC is to develop a science-based advanced safety simulation capability by utilizing existing safety simulation tools coupled with emerging high-fidelity modeling capabilities in a multi-resolution approach. As part of this integration, an existing whole-plant systems analysis code has been coupled with a high-fidelity computational fluid dynamics code to assess the impact of high-fidelity simulations on safety-related performance. With the coupled capabilities, it is possible to identify critical safety-related phenomenon in advanced reactor designs that cannot be resolved with existing tools. In this report, the impact of coupling is demonstrated by evaluating the conditions of outlet plenum thermal stratification during a protected loss of flow transient. Outlet plenum stratification was …
Date: May 31, 2010
Creator: Fanning, T. H.; Thomas, J. W. & Division, Nuclear Engineering
Object Type: Report
System: The UNT Digital Library
Characterization of the role of Fhit in maintenance of genomic integrity following low dose radiation, in vivo and in vitro (open access)

Characterization of the role of Fhit in maintenance of genomic integrity following low dose radiation, in vivo and in vitro

The major goal of this study is to determine the effects of the Fhit pathway on low dose ({le} 0.1 Gy) ionizing radiation (IR)-induced genetic instability. Reduction of Fhit protein expression is observed in most solid tumors particularly in those tumors resulting from exposure to environmental carcinogens. Therefore, characterization of the role of the Fhit-dependent pathway in preventing low dose IR-induced genetic instability will provide useful parameters for evaluating the low dose IR-induced risk of mutagenesis and carcinogenesis. We pursued 3 specific aims to study our hypothesis that the Fhit-dependent pathways maintain genomic integrity through adjusting checkpoint response and repair genes expression following low dose IR. Aim 1: Determine whether Fhit interaction with RPA is necessary for Fhit to affect the cellular response to low dose IR. We combined the approaches of in vitro (GST pull-down and site-directed mutagenesis) and in vivo (observing the co-localization and immunoprecipitation of Fhit and RPA in Fhit knock out mouse cells transfected with mutant Fhit which has lost ability to interact with RPA in vitro). Aim 2: Determine the role of genes whose expression is affected by Fhit in low dose irradiated cells. We analyzed the distinct signature of gene expression in low dose …
Date: May 31, 2010
Creator: Wang, Ya
Object Type: Report
System: The UNT Digital Library