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Supply Chain Security: CBP Works with International Entities to Promote Global Customs Security Standards and Initiatives, but Challenges Remain (open access)

Supply Chain Security: CBP Works with International Entities to Promote Global Customs Security Standards and Initiatives, but Challenges Remain

A letter report issued by the Government Accountability Office with an abstract that begins "Oceangoing cargo containers play a vital role in global trade but can also pose a risk of terrorist exploitation. U.S. Customs and Border Protection (CBP), part of the Department of Homeland Security (DHS), oversees security of the supply chain--the flow of goods from manufacturer to retailer. CBP anticipates that adoption of uniform, international customs security standards could eventually lead to a system of mutual recognition whereby the customs security-related practices and programs taken by one customs administration are recognized and accepted by another administration. In response to congressional requesters, GAO determined (1) actions CBP has taken to develop and implement international supply chain security standards, (2) actions CBP has taken with international partners to achieve mutual recognition of customs security practices, and (3) issues CBP and foreign customs administrations anticipate in implementing 100 percent scanning of U.S.-bound container cargo. To conduct its work, GAO analyzed CBP documents on supply chain security programs and international cooperation initiatives and met with CBP officials and foreign customs officials from various trading partner nations. Also, GAO drew upon its related reports and testimony on supply chain security issued earlier this …
Date: August 15, 2008
Creator: United States. Government Accountability Office.
Object Type: Report
System: The UNT Digital Library
Defense Critical Infrastructure: Adherence to Guidance Would Improve DOD's Approach to Identifying and Assuring the Availability of Critical Transportation Assets (open access)

Defense Critical Infrastructure: Adherence to Guidance Would Improve DOD's Approach to Identifying and Assuring the Availability of Critical Transportation Assets

A letter report issued by the Government Accountability Office with an abstract that begins "The Department of Defense (DOD) established the Defense Critical Infrastructure Program (DCIP) to assure the availability of mission-critical infrastructure, including surface, sea, and air transportation assets to carry out its missions. GAO was asked to evaluate (1) the extent to which the U.S. Transportation Command (TRANSCOM) has identified, prioritized, and assessed critical transportation assets; (2) the extent to which DOD installation personnel have taken actions to help assure the availability of critical transportation assets, both within and independent of DCIP; and (3) how DOD is funding critical transportation asset assurance. GAO examined a nonprojectable sample of 22 critical transportation assets, reviewed relevant DOD guidance and documents, and interviewed cognizant officials."
Date: August 15, 2008
Creator: United States. Government Accountability Office.
Object Type: Report
System: The UNT Digital Library
Tax Preparers: Oregon's Regulatory Regime May Lead to Improved Federal Tax Return Accuracy and Provides a Possible Model for National Regulation (open access)

Tax Preparers: Oregon's Regulatory Regime May Lead to Improved Federal Tax Return Accuracy and Provides a Possible Model for National Regulation

A letter report issued by the Government Accountability Office with an abstract that begins "Millions of taxpayers use paid tax return preparers and many of these paid preparers are not subject to any qualification requirements. Paid preparers in California and Oregon are exceptions in that these states have set paid preparer qualification standards. Additionally, two bills before Congress would require national paid preparer regulations. To help Congress better understand the potential costs and revenue effects of regulating paid preparers, GAO was asked to study (1) how IRS, California, Oregon, and other states regulate paid preparers, (2) how the accuracy of federal tax returns from California and Oregon compare to other returns, and (3) state-level costs and benefits of the California and Oregon programs and insights they provide for a possible national program. GAO analyzed IRS research data on tax return accuracy; interviewed IRS officials, state administrators, and preparer community representatives; and reviewed relevant documents."
Date: August 15, 2008
Creator: United States. Government Accountability Office.
Object Type: Report
System: The UNT Digital Library
Defense Acquisitions: Department of Defense Needs Framework for Balancing Investments in Tactical Radios (open access)

Defense Acquisitions: Department of Defense Needs Framework for Balancing Investments in Tactical Radios

A letter report issued by the Government Accountability Office with an abstract that begins "The Department of Defense (DOD) has spent an estimated $12 billion on the development and production of tactical radios over the last 5 years--about as much as was spent producing Virginia Class submarines ($10.8 billion) in the same period. Survivability and lethality in warfare are increasingly dependent on superior information and communication capabilities. DOD is counting on the Joint Tactical Radio System (JTRS) to deliver the breakthroughs in this area. At the same time as it is developing JTRS, DOD wants to ensure that current forces are equipped with sufficient legacy radios. To address Subcommittee concerns regarding DOD's approach to meeting current and future radio needs, GAO examined this subject. In response, this report addresses (1) how planned investments in key tactical radio systems changed over the last 5 years, (2) why these changes occurred, and (3) the challenges ahead. To assess these topics, GAO's work included reviewing 2003 through 2007 budget requests, legacy radio procurements, and the status of the JTRS program; and interviewing officials from DOD organizations."
Date: August 15, 2008
Creator: United States. Government Accountability Office.
Object Type: Report
System: The UNT Digital Library
Review of the Transportation Security Administration's Air Cargo Screening Exemptions Report (open access)

Review of the Transportation Security Administration's Air Cargo Screening Exemptions Report

Correspondence issued by the Government Accountability Office with an abstract that begins "This report formally transmits the attached briefing in response to section 1602(b) of the Implementing Recommendations of the 9/11 Commission Act of 2007. The act requires the Comptroller General to provide an assessment of the methodology used by the Secretary of Homeland Security for maintaining, changing or eliminating an exemption under 49 U.S.C. 44901(i)(1). We provided the mandated reporting committees with a restricted version of this briefing in July 2008. Based on the results of our review, we are not making any recommendations for congressional consideration or agency action. All information considered Sensitive Security Information by the Transportation Security Administration has been removed from this product."
Date: August 15, 2008
Creator: United States. Government Accountability Office.
Object Type: Text
System: The UNT Digital Library
Military Personnel: Improvements Needed to Increase Effectiveness of DOD's Programs to Promote Positive Working Relationships between Reservists and Their Employers (open access)

Military Personnel: Improvements Needed to Increase Effectiveness of DOD's Programs to Promote Positive Working Relationships between Reservists and Their Employers

Correspondence issued by the Government Accountability Office with an abstract that begins "Since September 11, 2001, the Department of Defense (DOD) has relied on more than 650,000 members of the National Guard and Reserve to support operations at home and abroad. As demobilized reservists return to civilian life and their civilian employment, the difficulties some face in maintaining positive working relationships with their employers is an area of interest. Maintaining employers' continued support for their reservist employees will be critical if DOD is to retain experienced reservists in these times of longer and more frequent deployments. The employment and reemployment rights of service members as they transition between their federal duties and their civilian employment are governed by the Uniformed Services Employment and Reemployment Rights Act (USERRA) of 1994. Education of reservists and employers about USERRA is primarily conducted through DOD's office of Employer Support of the Guard and Reserve (ESGR), which serves as DOD's focal point in managing the department's relations with reservists and their civilian employers. The Military Reservists and Veteran Small Business Reauthorization and Opportunity Act of 20083 directed the Comptroller General of the United States to report to the Senate Committee on Armed Services, the Senate …
Date: August 15, 2008
Creator: United States. Government Accountability Office.
Object Type: Text
System: The UNT Digital Library
State-of-the-Art Beta Detection and Dosimetry (open access)

State-of-the-Art Beta Detection and Dosimetry

The research funded by this NEER grant establishes the framework for a detailed understanding of the challenges in beta dosimetry, especially in the presence of a mixed radiation field. The work also stimulated the thinking of the research group which will lead to new concepts in digital signal processing to allow collection of detection signals and real-time analysis such that simultaneous beta and gamma spectroscopy can take place. The work described herein (with detail in the many publications that came out of this research) was conducted in a manner that provided dissertation and thesis topics for three students, one of whom was completely funded by this grant. The overall benefit of the work came in the form of a dramatic shift in signal processing that is normally conducted in analog pulse shape analysis. Analog signal processing was shown not to be feasible for this type of work; digital signal processing was a must. This, in turn, led the research team to a new understanding of pulse analysis, one in which expands the state-of-the-art in simultaneous beta and gamma spectroscopy with a single detector.
Date: August 15, 2008
Creator: Hamby, David M.
Object Type: Report
System: The UNT Digital Library
Poroelastic modeling of fracture-seismic wave interaction (open access)

Poroelastic modeling of fracture-seismic wave interaction

Rock containing a compliant, fluid-filled fracture can be viewed as one case of heterogeneous poroelastic media. When this fracture is subjected to seismic waves, a strong contrast in the elastic stiffness between the fracture itself and the background can result in enhanced grain-scale local fluid flow. Because this flow--relaxing the pressure building up within the fracture--can increase the dynamic compliance of the fracture and change energy dissipation (attenuation), the scattering of seismic waves can be enhanced. Previously, for a flat, infinite fracture, we derived poroelastic seismic boundary conditions that describe the relationship between a finite jump in the stress and displacement across a fracture, expressed as a function of the stress and displacement at the boundaries. In this paper, we use these boundary conditions to determine frequency-dependent seismic wave transmission and reflection coefficients. Fluid-filled fractures with a range of mechanical and hydraulic properties are examined. From parametric studies, we found that the hydraulic permeability of a fracture fully saturated with water has little impact on seismic wave scattering. In contrast, the seismic response of a partially water-saturated fracture and a heterogeneous fracture filled with compliant liquid (e.g., supercritical CO{sub 2}) depended on the fracture permeability.
Date: August 15, 2008
Creator: Nakagawa, Seiji
Object Type: Article
System: The UNT Digital Library
Measurements of Branching Fractions for B+ -> rho+ gamma, B0 -> rho0 gamma, and B0 -> omega gamma (open access)

Measurements of Branching Fractions for B+ -> rho+ gamma, B0 -> rho0 gamma, and B0 -> omega gamma

The authors present branching fraction measurements for the radiative decays B{sup +} {yields} {rho}{sup +}{gamma}, B{sup 0} {yields} {rho}{sup 0}{gamma}, and B{sup 0} {yields} {omega}{gamma}. The analysis is based on a data sample of 465 million B{bar B} events collected with the BABAR detector at the PEP-II asymmetric-energy B Factory located at the Stanford Linear Accelerator Center (SLAC). They find {Beta}(B{sup +} {yields} {rho}{sup +}{gamma}) = (1.20{sub -0.37}{sup +0.42} {+-} 0.20) x 10{sup -6}, {Beta}(B{sup 0} {yields} {rho}{sup 0}{gamma}) = (0.97{sub -0.22}{sup +0.24} {+-} 0.06) x 10{sup -6}, and a 90% C.L. upper limit {Beta}(B{sup 0} {yields} {omega}{gamma}) < 0.9 x 10{sup -6}, where the first error is statistical and the second is systematic. They also measure the isospin-violating quantity {Lambda}(B{sup +} {yields} {rho}{sup +}{gamma})/2{Lambda}(B{sup 0} {yields} {rho}{sup 0}{gamma}) - 1 = -0.43{sub -0.22}{sup +0.25} {+-} 0.10.
Date: August 15, 2008
Creator: Aubert, B.
Object Type: Article
System: The UNT Digital Library
REAL TIME SYSTEM OPERATIONS 2006-2007 (open access)

REAL TIME SYSTEM OPERATIONS 2006-2007

The Real Time System Operations (RTSO) 2006-2007 project focused on two parallel technical tasks: (1) Real-Time Applications of Phasors for Monitoring, Alarming and Control; and (2) Real-Time Voltage Security Assessment (RTVSA) Prototype Tool. The overall goal of the phasor applications project was to accelerate adoption and foster greater use of new, more accurate, time-synchronized phasor measurements by conducting research and prototyping applications on California ISO's phasor platform - Real-Time Dynamics Monitoring System (RTDMS) -- that provide previously unavailable information on the dynamic stability of the grid. Feasibility assessment studies were conducted on potential application of this technology for small-signal stability monitoring, validating/improving existing stability nomograms, conducting frequency response analysis, and obtaining real-time sensitivity information on key metrics to assess grid stress. Based on study findings, prototype applications for real-time visualization and alarming, small-signal stability monitoring, measurement based sensitivity analysis and frequency response assessment were developed, factory- and field-tested at the California ISO and at BPA. The goal of the RTVSA project was to provide California ISO with a prototype voltage security assessment tool that runs in real time within California ISO?s new reliability and congestion management system. CERTS conducted a technical assessment of appropriate algorithms, developed a prototype incorporating state-of-art …
Date: August 15, 2008
Creator: Eto, Joseph H.; Parashar, Manu & Lewis, Nancy Jo
Object Type: Report
System: The UNT Digital Library
Search For B --> K* Nu Anti-Nu Decays (open access)

Search For B --> K* Nu Anti-Nu Decays

We present a search for the decays B {yields} K* {nu}{bar {nu}} using 454 x 10{sup 6} B{bar B} pairs collected at the {Upsilon}(4S) resonance with the BABAR detector at the SLAC PEP-II B-Factory. We first select an event sample where one B is reconstructed in a semileptonic or hadronic mode with one charmed meson. The remaining particles in the event are then examined to search for a B {yields} K* {nu}{bar {nu}} decay. The charged K* is reconstructed as K*{sup +} {yields} K{sub S}{sup 0}{pi}{sup +} or K*{sup +} {yields} K{sup +}{pi}{sup 0}; the neutral K* is identified in K*{sup 0} {yields} K{sup +}{pi}{sup -} mode. We establish upper limits at 90% confidence level of {Beta}(B{sup +} {yields} K*{sup +} {nu}{bar {nu}}) < 8 x 10{sup -5}, {Beta}(B{sup 0} {yields} K*{sup 0} {nu}{bar {nu}}) < 12 x 10{sup -5}, and {Beta}(B {yields} K* {nu}{bar {nu}}) < 8 x 10{sup -5}.
Date: August 15, 2008
Creator: Aubert, B.
Object Type: Article
System: The UNT Digital Library
Spectroscopic Confirmation of the Fifth Image of SDSS J1004+4112 and Implications for the M_BH-sigma_* Relation at z=0.68 (open access)

Spectroscopic Confirmation of the Fifth Image of SDSS J1004+4112 and Implications for the M_BH-sigma_* Relation at z=0.68

We present the results of deep spectroscopy for the central region of the cluster lens SDSS J1004+4112 with the Subaru telescope. A secure detection of an emission line of the faint blue stellar object (component E) near the center of the brightest cluster galaxy (G1) confirms that it is the central fifth image of the lensed quasar system. In addition, we measure the stellar velocity dispersion of G1 to be {sigma}{sub *} = 352 {+-} 13 km s{sup -1}. We combine these results to obtain constraints on the mass M{sub BH} of the putative black hole (BH) at the center of the inactive galaxy G1, and hence on the M{sub BH}-{sigma}{sub *} relation at the lens redshift z{sub l} = 0.68. From detailed mass modeling, we place an upper limit on the black hole mass, M{sub BH} < 2.1 x 10{sup 10} M{sub {circle_dot}} at 1{sigma} level (< 3.1 x 10{sup 10}M{sub {circle_dot}} at 3{sigma}), which is consistent with black hole masses expected from the local and redshift-evolved M{sub BH}-{sigma}{sub *} relations, M{sub BH} {approx} 10{sup 9}-10{sup 10} M{sub {circle_dot}}.
Date: August 15, 2008
Creator: Inada, Naohisa; Oguri, Masamune; Falco, Emilio E.; Broadhurst, Tom J.; Ofek, Eran O.; Kochanek, Christopher S. et al.
Object Type: Article
System: The UNT Digital Library
Sequestering the Gravitino: Neutralino Dark Matter in Gauge Mediation (open access)

Sequestering the Gravitino: Neutralino Dark Matter in Gauge Mediation

In conventional models of gauge-mediated supersymmetry breaking, the lightest supersymmetric particle (LSP) is invariably the gravitino. However, if the supersymmetry breaking sector is strongly coupled, conformal sequestering may raise the mass of the gravitino relative to the remaining soft supersymmetry-breaking masses. In this letter, we demonstrate that such conformal dynamics in gauge-mediated theories may give rise to satisfactory neutralino dark matter while simultaneously solving the flavor and {mu}/B{mu} problems.
Date: August 15, 2008
Creator: Craig, Nathaniel J.; /Stanford U., Dept. Phys.; Green, Daniel & /SLAC /Stanford U., Dept. Phys.
Object Type: Article
System: The UNT Digital Library
Monodisperse Platinum and Rhodium Nanoparticles as Model Heterogeneous Catalysts (open access)

Monodisperse Platinum and Rhodium Nanoparticles as Model Heterogeneous Catalysts

Model heterogeneous catalysts have been synthesized and studied to better understand how the surface structure of noble metal nanoparticles affects catalytic performance. In this project, monodisperse rhodium and platinum nanoparticles of controlled size and shape have been synthesized by solution phase polyol reduction, stabilized by polyvinylpyrrolidone (PVP). Model catalysts have been developed using these nanoparticles by two methods: synthesis of mesoporous silica (SBA-15) in the presence of nanoparticles (nanoparticle encapsulation, NE) to form a composite of metal nanoparticles supported on SBA-15 and by deposition of the particles onto a silicon wafer using Langmuir-Blodgett (LB) monolayer deposition. The particle shapes were analyzed by transmission electron microscopy (TEM) and high resolution TEM (HRTEM) and the sizes were determined by TEM, X-ray diffraction (XRD), and in the case of NE samples, room temperature H2 and CO adsorption isotherms. Catalytic studies were carried out in homebuilt gas-phase reactors. For the nanoparticles supported on SBA-15, the catalysts are in powder form and were studied using the homebuilt systems as plug-flow reactors. In the case of nanoparticles deposited on silicon wafers, the same systems were operated as batch reactors. This dissertation has focused on the synthesis, characterization, and reaction studies of model noble metal heterogeneous catalysts. …
Date: August 15, 2008
Creator: Coble, Inger M
Object Type: Thesis or Dissertation
System: The UNT Digital Library
Improving Estimation Accuracy of Aggregate Queries on Data Cubes (open access)

Improving Estimation Accuracy of Aggregate Queries on Data Cubes

In this paper, we investigate the problem of estimation of a target database from summary databases derived from a base data cube. We show that such estimates can be derived by choosing a primary database which uses a proxy database to estimate the results. This technique is common in statistics, but an important issue we are addressing is the accuracy of these estimates. Specifically, given multiple primary and multiple proxy databases, that share the same summary measure, the problem is how to select the primary and proxy databases that will generate the most accurate target database estimation possible. We propose an algorithmic approach for determining the steps to select or compute the source databases from multiple summary databases, which makes use of the principles of information entropy. We show that the source databases with the largest number of cells in common provide the more accurate estimates. We prove that this is consistent with maximizing the entropy. We provide some experimental results on the accuracy of the target database estimation in order to verify our results.
Date: August 15, 2008
Creator: Pourabbas, Elaheh & Shoshani, Arie
Object Type: Article
System: The UNT Digital Library
Final report: results of the 2007 investigation of potential contamination at the former CCC/USDA facility in Powhattan, Kansas. (open access)

Final report: results of the 2007 investigation of potential contamination at the former CCC/USDA facility in Powhattan, Kansas.

The 2007 investigation of carbon tetrachloride and chloroform contamination at Powhattan, Kansas, was conducted at the request of the Kansas Department of Health and Environment (KDHE 2006a). The Environmental Science Division of Argonne National Laboratory implemented the investigation on behalf of the Commodity Credit Corporation of the U.S. Department of Agriculture (CCC/USDA). The primary purposes of the investigation were to evaluate potential contaminant source areas on the former CCC/USDA property, determine the horizontal and vertical extent of potential contamination, conduct groundwater monitoring, and provide recommendations for future action.
Date: August 15, 2008
Creator: LaFreniere, L. M.
Object Type: Report
System: The UNT Digital Library
Study of the pi+pi-J//psi Mass Spectrum Via Initial State Radiation at BaBar (open access)

Study of the pi+pi-J//psi Mass Spectrum Via Initial State Radiation at BaBar

We present an update of the study of the Y(4260) resonance, produced in the process e{sup +}e{sup -} {yields} {gamma}{sub ISR} {pi}{sup +}{pi}{sup -} J/{psi} using initial-state radiation events at the PEP-II e{sup +}e{sup -} storage rings. This study is based on 454 fb{sup -1} of data recorded with the BABAR detector at a center-of-mass energy in the {Upsilon}(4S) resonance region. From a fit with a single non-relativistic Breit-Wigner shape we obtain updated parameters for the Y(4260) resonance which are m{sub Y} = 4252 {+-} 6{sub -3}{sup +2} MeV/c{sup 2} and {Lambda}{sub Y} = 105 {+-} 18{sub -6}{sup +4} MeV/c{sup 2}; we also measure {Beta}({pi}{sup +}{pi}{sup -} J/{psi}){Lambda}{sub e{sup +}e{sup -}} = (7.5 {+-} 0.9 {+-} 0.8) eV. We cannot confirm the recent BELLE observation of a broad structure around 4.05GeV/c{sup 2} in this decay mode.
Date: August 15, 2008
Creator: Aubert, Bernard
Object Type: Article
System: The UNT Digital Library
Greensheet (Houston, Tex.), Vol. 39, No. 334, Ed. 1 Friday, August 15, 2008 (open access)

Greensheet (Houston, Tex.), Vol. 39, No. 334, Ed. 1 Friday, August 15, 2008

Free weekly newspaper that includes business and classified advertising.
Date: August 15, 2008
Creator: unknown
Object Type: Newspaper
System: The Portal to Texas History
Greensheet (Houston, Tex.), Vol. 39, No. 336, Ed. 1 Friday, August 15, 2008 (open access)

Greensheet (Houston, Tex.), Vol. 39, No. 336, Ed. 1 Friday, August 15, 2008

Free weekly newspaper that includes business and classified advertising.
Date: August 15, 2008
Creator: unknown
Object Type: Newspaper
System: The Portal to Texas History
Brief Summaries of Federal Animal Protection Statutes (open access)

Brief Summaries of Federal Animal Protection Statutes

This report contains brief summaries of federal animal protection statutes, listed alphabetically. It includes statutes that allow the disabled to use service animals, statutes aimed at acts of animal rights advocates (the Animal Enterprise Protection Act of 1992, and the Recreational Hunting Safety and Preservation Act of 1994), as well as other statutes concerning animal protection and conservation.
Date: August 15, 2008
Creator: Cohen, Henry
Object Type: Report
System: The UNT Digital Library
Dallas Voice (Dallas, Tex.), Vol. 25, No. 13, Ed. 1 Friday, August 15, 2008 (open access)

Dallas Voice (Dallas, Tex.), Vol. 25, No. 13, Ed. 1 Friday, August 15, 2008

Weekly newspaper from Dallas, Texas that includes local, state, and national news and advertising of interest to the Lesbian, Gay, Bisexual, and Transgender (LGBT) community.
Date: August 15, 2008
Creator: Nash, Tammye
Object Type: Newspaper
System: The UNT Digital Library
FY-2007 PNNL Voluntary Protection Program (VPP) Program Evaluation (open access)

FY-2007 PNNL Voluntary Protection Program (VPP) Program Evaluation

This document reports the results of the FY-2007 PNNL VPP Program Evaluation, which is a self-assessment of the operational and programmatic performance of the Laboratory related to worker safety and health. The report was compiled by a team of worker representatives and safety professionals who evaluated the Laboratory's worker safety and health programs on the basis of DOE-VPP criteria. The principle elements of DOE's VPP program are: Management Leadership, Employee Involvement, Worksite Analysis, Hazard Prevention and Control, and Safety and Health Training.
Date: August 15, 2008
Creator: Wright, Patrick A.; Fisher, Julie A.; Goheen, Steven C.; Isern, Nancy G.; Madson, Vernon J.; Meicenheimer, Russell L. et al.
Object Type: Report
System: The UNT Digital Library
Greensheet (Houston, Tex.), Vol. 39, No. 335, Ed. 1 Friday, August 15, 2008 (open access)

Greensheet (Houston, Tex.), Vol. 39, No. 335, Ed. 1 Friday, August 15, 2008

Free weekly newspaper that includes business and classified advertising.
Date: August 15, 2008
Creator: unknown
Object Type: Newspaper
System: The Portal to Texas History
Design and analysis of mismatch probes for long oligonucleotide microarrays (open access)

Design and analysis of mismatch probes for long oligonucleotide microarrays

Nonspecific hybridization is currently a major concern with microarray technology. One of most effective approaches to estimating nonspecific hybridizations in oligonucleotide microarrays is the utilization of mismatch probes; however, this approach has not been used for longer oligonucleotide probes. Here, an oligonucleotide microarray was constructed to evaluate and optimize parameters for 50-mer mismatch probe design. A perfect match (PM) and 28 mismatch (MM) probes were designed for each of ten target genes selected from three microorganisms. The microarrays were hybridized with synthesized complementary oligonucleotide targets at different temperatures (e.g., 42, 45 and 50 C). In general, the probes with evenly distributed mismatches were more distinguishable than those with randomly distributed mismatches. MM probes with 3, 4 and 5 mismatched nucleotides were differentiated for 50-mer oligonucleotide probes hybridized at 50, 45 and 42 C, respectively. Based on the experimental data generated from this study, a modified positional dependent nearest neighbor (MPDNN) model was constructed to adjust the thermodynamic parameters of matched and mismatched dimer nucleotides in the microarray environment. The MM probes with four flexible positional mismatches were designed using the newly established MPDNN model and the experimental results demonstrated that the redesigned MM probes could yield more consistent hybridizations. Conclusions: …
Date: August 15, 2008
Creator: Deng, Ye; He, Zhili; Van Nostrand, Joy D. & Zhou, Jizhong
Object Type: Article
System: The UNT Digital Library