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Afghanistan Drug Control: Despite Improved Efforts, Deteriorating Security Threatens Success of U.S. Goals (open access)

Afghanistan Drug Control: Despite Improved Efforts, Deteriorating Security Threatens Success of U.S. Goals

A letter report issued by the Government Accountability Office with an abstract that begins "The prevalence of opium poppy cultivation and drug trafficking in Afghanistan imperils the stability of its government and threatens to turn the conflict-ridden nation once again into a safe haven for traffickers and terrorists. To combat the drug trade, the U.S. government developed a counternarcotics strategy consisting of five pillars--alternative livelihoods, elimination and eradication, interdiction, law enforcement and justice, and public information. The Emergency Supplemental Appropriations Act of 2005 directed GAO to examine the use of all fiscal year 2005 funds administered by the U.S. Agency for International Development (USAID) and Department of State (State) for Afghan counternarcotics programs. To comply with this mandate, we examined progress under each counternarcotics pillar, challenges faced, and efforts to ensure that funds were used for intended purposes. To address these objectives, GAO reviewed pertinent USAID and State documents and met with cognizant U.S. and international officials in Washington, D.C., and Afghanistan. GAO makes no recommendations in this report. USAID, State, Department of Defense, and Department of Justice were provided a draft of this report, but did not provide formal comments."
Date: November 15, 2006
Creator: United States. Government Accountability Office.
Object Type: Report
System: The UNT Digital Library
Mail Security: Incidents at DOD Mail Facilities Exposed Problems That Require Further Actions (open access)

Mail Security: Incidents at DOD Mail Facilities Exposed Problems That Require Further Actions

A letter report issued by the Government Accountability Office with an abstract that begins "In March 2005, two well-publicized and nearly simultaneous incidents involving the suspicion of anthrax took place in the Washington, D.C., area. The incidents occurred at Department of Defense (DOD) mail facilities at the Pentagon and at a commercial office complex (Skyline Complex). While these incidents were false alarms, DOD and other federal and local agencies responded. The Postal Service suspended operations at two of its facilities and over a thousand DOD and Postal Service employees were given antibiotics as a precaution against their possible exposure to anthrax. This report describes (1) what occurred at the Pentagon and Skyline Complex mail facilities, (2) the problems we identified in detecting and responding to the incidents, (3) the actions taken by DOD that address the problems that occurred, and (4) the extent to which DOD's actions address the problems."
Date: September 15, 2006
Creator: United States. Government Accountability Office.
Object Type: Report
System: The UNT Digital Library
National Flood Insurance Program: New Processes Aided Hurricane Katrina Claims Handling, but FEMA's Oversight Should Be Improved (open access)

National Flood Insurance Program: New Processes Aided Hurricane Katrina Claims Handling, but FEMA's Oversight Should Be Improved

A letter report issued by the Government Accountability Office with an abstract that begins "In August and September 2005, Hurricanes Katrina and Rita caused unprecedented destruction to property along the Gulf Coast, resulting in billions of dollars of damage claims to the National Flood Insurance Program (NFIP). This report, which we initiated under the authority of the Comptroller General, examines (1) the impact of Hurricanes Katrina and Rita on the NFIP and paid losses by location and property type; (2) the challenges the Federal Emergency Management Agency (FEMA) and others faced in addressing the needs of NFIP claimants and communities; (3) FEMA's methods of monitoring and overseeing claims adjustments; and (4) FEMA's efforts to meet the requirements of the Flood Insurance Reform Act of 2004 to establish policyholder coverage notifications, an appeals process for claimants, and education and training requirements for agents. To conduct these assessments, GAO interviewed FEMA and insurance officials, analyzed claims data, and examined a sample of reports done on the accuracy of claims adjustments."
Date: December 15, 2006
Creator: United States. Government Accountability Office.
Object Type: Report
System: The UNT Digital Library
Information Technology: Agencies and OMB Should Strengthen Processes for Identifying and Overseeing High Risk Projects (open access)

Information Technology: Agencies and OMB Should Strengthen Processes for Identifying and Overseeing High Risk Projects

A letter report issued by the Government Accountability Office with an abstract that begins "In August 2005, the Office of Management and Budget (OMB) issued a memorandum directing agencies to identify high risk information technology (IT) projects and provide quarterly reports on those with performance shortfalls--projects that did not meet criteria established by OMB. GAO was asked to (1) provide a summary identifying by agency the number of high risk projects, their proposed budget for fiscal year 2007, agency reasons for the high risk designation, and reported performance shortfalls; (2) determine how high risk projects were identified and updated and what processes and procedures have been established to effectively oversee them; and (3) determine the relationship between the high risk list and OMB's Management Watch List--those projects that OMB determines need improvements associated with key aspects of their budget justifications."
Date: June 15, 2006
Creator: United States. Government Accountability Office.
Object Type: Report
System: The UNT Digital Library
Tax Gap: Making Significant Progress in Improving Tax Compliance Rests on Enhancing Current IRS Techniques and Adopting New Legislative Actions (open access)

Tax Gap: Making Significant Progress in Improving Tax Compliance Rests on Enhancing Current IRS Techniques and Adopting New Legislative Actions

Testimony issued by the Government Accountability Office with an abstract that begins "The Internal Revenue Service's (IRS) most recent estimate of the difference between what taxpayers timely and accurately paid in taxes and what they owed was $345 billion. IRS estimates it will eventually recover some of this tax gap, resulting in an estimated net tax gap of $290 billion. The tax gap arises when taxpayers fail to comply with the tax laws by underreporting tax liabilities on tax returns; underpaying taxes due from filed returns; or nonfiling, which refers to the failure to file a required tax return altogether or in a timely manner. The Chairman and Ranking Minority Member of the Senate Committee on the Budget asked GAO to present information on the causes of and possible solutions to the tax gap. This testimony addresses the nature and extent of the tax gap and the significance of reducing the tax gap, including some steps that may assist with this challenging task. For context, this testimony also addressed GAO's most recent simulations of the long-term fiscal outlook and the need for a fundamental reexamination of major spending and tax policies and priorities."
Date: February 15, 2006
Creator: United States. Government Accountability Office.
Object Type: Text
System: The UNT Digital Library
Public Housing: Information on the Roles of HUD, Public Housing Agencies, Capital Markets, and Service Organizations (open access)

Public Housing: Information on the Roles of HUD, Public Housing Agencies, Capital Markets, and Service Organizations

Testimony issued by the Government Accountability Office with an abstract that begins "Under the Public Housing Program, the Department of Housing and Urban Development (HUD) and local public housing agencies (PHA) provide housing for low-income residents at rents they can afford. Today, over 3,000 PHAs administer approximately 1.2 million public housing units throughout the nation. First authorized in 1937, the program has undergone changes over the decades. The Quality Housing and Work Responsibility Act of 1998 increased managerial flexibility but also established new requirements for housing agencies. Some observers have questioned the program's ability to provide quality, affordable housing to the nation's neediest families. This testimony, which is based upon a number of reports that GAO has issued related to public housing since 2002, discusses the roles of (1) HUD (2) public housing agencies, (3) capital markets, and (4) community services organizations in the public housing system."
Date: February 15, 2006
Creator: United States. Government Accountability Office.
Object Type: Text
System: The UNT Digital Library
Health Information Technology: HHS is Continuing Efforts to Define a National Strategy (open access)

Health Information Technology: HHS is Continuing Efforts to Define a National Strategy

Testimony issued by the Government Accountability Office with an abstract that begins "As GAO and others have reported, the use of information technology (IT) has enormous potential to improve the quality of health care and is critical to improving the performance of the U.S. health care system. Given the federal government's influence in the health care industry, it has been urged over the years to take a leadership role in driving change to improve the quality and effectiveness of medical care, including the adoption of IT. In April 2004, President Bush called for widespread adoption of interoperable electronic health records within 10 years; established the position of the National Coordinator for Health IT, who was appointed in May 2004 and released a framework for strategic action two months later. In May 2005, GAO recommended that HHS establish detailed plans and milestones for each phase of the framework and take steps to ensure that those plans are followed and milestones are met. HHS agreed with our recommendation. GAO (1) assessed the progress being made by the Department of Health and Human Services (HHS) since 2005 to develop a national health IT strategy and (2) provided an overview of selected federal agencies' …
Date: March 15, 2006
Creator: United States. Government Accountability Office.
Object Type: Text
System: The UNT Digital Library
21st Century: Addressing Long-Term Fiscal Challenges Must Include a Re-examination of Mandatory Spending (open access)

21st Century: Addressing Long-Term Fiscal Challenges Must Include a Re-examination of Mandatory Spending

Testimony issued by the Government Accountability Office with an abstract that begins "This testimony discusses entitlement and other mandatory spending programs in light of our nation's long-term fiscal outlook and the challenges it poses for the budget and oversight processes. In our report entitled 21st Century Challenges: Reexamining the Base of the Federal Government, we presented illustrative questions for policy makers to consider as they carry out their responsibilities. These questions look across major areas of the budget and federal operations including discretionary and mandatory spending, and tax policies and programs. We hope that this report, among other things, will be used by various congressional committees as they consider which areas of government need particular attention and reconsideration. Congress will also receive more specific proposals, some of them will be presented within comprehensive agendas. Our report provides examples of the kinds of difficult choices the nation faces with regard to discretionary spending; mandatory spending, including entitlements; as well as tax policies and compliance activities."
Date: February 15, 2006
Creator: United States. Government Accountability Office.
Object Type: Text
System: The UNT Digital Library
High Risk Series: GAO's High-Risk Program (open access)

High Risk Series: GAO's High-Risk Program

Testimony issued by the Government Accountability Office with an abstract that begins "GAO audits and evaluations identify federal programs and operations that in some cases are high risk due to their greater vulnerabilities to fraud, waste, abuse, and mismanagement. Increasingly, GAO also has identified high-risk areas that are in need of broad-based transformations to address major economy, efficiency, or effectiveness challenges. Since 1990 with each new Congress, GAO has reported on its high-risk list. GAO's most recent update, in January 2005, presented the 109th Congress with the latest status of existing and new high-risk areas warranting attention by both the Congress and the administration. Lasting solutions to high-risk problems offer the potential to save billions of dollars, dramatically improve service to the American public, strengthen public confidence and trust in the performance and accountability of our national government, and ensure the ability of government to deliver on its promises."
Date: March 15, 2006
Creator: United States. Government Accountability Office.
Object Type: Text
System: The UNT Digital Library
VA Health Care: Patient Safety Could be Enhanced by Improvements in Employment Screening and Physician Privileging Practices (open access)

VA Health Care: Patient Safety Could be Enhanced by Improvements in Employment Screening and Physician Privileging Practices

Testimony issued by the Government Accountability Office with an abstract that begins "In its March 2004 report, "VA Health Care: Improved Screening of Practitioners Would Reduce Risk to Veterans," GAO-04-566, GAO made recommendations to improve VA's employment screening of practitioners. GAO was asked to testify today on steps VA has taken to improve its employment screening requirements and VA's physician credentialing and privileging processes because of their importance to patient safety. This testimony is based on two GAO reports released today that determined the extent to which (1) VA has taken steps to improve employment screening for practitioners by implementing GAO's 2004 recommendations, (2) VA facilities are in compliance with selected credentialing and privileging requirements for physicians, and (3) VA has internal controls to help ensure the accuracy of privileging information."
Date: June 15, 2006
Creator: United States. Government Accountability Office.
Object Type: Text
System: The UNT Digital Library
Nuclear Regulatory Commission: Preliminary Observations on Its Oversight to Ensure the Safe Operation of Nuclear Power Plants (open access)

Nuclear Regulatory Commission: Preliminary Observations on Its Oversight to Ensure the Safe Operation of Nuclear Power Plants

Testimony issued by the Government Accountability Office with an abstract that begins "The Nuclear Regulatory Commission (NRC) has the responsibility to provide oversight to ensure that the nation's 103 commercial nuclear power plants are operated safely. While the safety of these plants has always been important, since radioactive release could harm the public and the environment, NRC's oversight has become even more critical as the Congress and the nation consider the potential resurgence of nuclear power in helping to meet the nation's growing energy needs. Prior to 2000, NRC was criticized for having a safety oversight process that was not always focused on the most important safety issues and in some cases, was overly subjective. To address these and other concerns, NRC implemented a new oversight process--the Reactor Oversight Process (ROP). NRC continues to modify the ROP to incorporate feedback from stakeholders and in response to other external events. This statement summarizes information on (1) how NRC oversees nuclear power plants, (2) the results of the ROP over the past several years, and (3) the aspects of the ROP that need improvement and the status of NRC's efforts to improve them. This statement discusses preliminary results of GAO's work. GAO …
Date: June 15, 2006
Creator: United States. Government Accountability Office.
Object Type: Text
System: The UNT Digital Library
Capitol Visitor Center: Status of Project's Schedule and Cost as of March 15, 2006 (open access)

Capitol Visitor Center: Status of Project's Schedule and Cost as of March 15, 2006

Testimony issued by the Government Accountability Office with an abstract that begins "This testimony discusses the Architect of the Capitol's progress in achieving selected project milestones and in managing the project's schedule since Congress's February 15, 2006, hearing on the project. As part of this discussion, we will address a number of key challenges and risks that continue to face the project, as well as actions AOC has taken or plans to take to address these risks. In addition, we will discuss the status of the project's costs and funding."
Date: March 15, 2006
Creator: United States. Government Accountability Office.
Object Type: Text
System: The UNT Digital Library
Capitol Visitor Center: Update on Status of Project's Schedule and Cost As of November 15, 2006 (open access)

Capitol Visitor Center: Update on Status of Project's Schedule and Cost As of November 15, 2006

Testimony issued by the Government Accountability Office with an abstract that begins "We are pleased to assist the Senate Committee on Appropriations, Subcommittee on the Legislative Branch in monitoring progress on the Capitol Visitor Center (CVC) project. Our remarks will focus on (1) the Architect of the Capitol's (AOC) construction progress and problems since the Subcommittee's September 21, 2006, hearing and their impact on the project's schedule; and (2) the project's expected cost at completion and funding situation. As part of this discussion, we will address a number of key challenges and risks that continue to face the project as well as actions AOC has recently taken, and plans or needs to take, to meet its currently scheduled completion date."
Date: November 15, 2006
Creator: United States. Government Accountability Office.
Object Type: Text
System: The UNT Digital Library
Agency Management of Contractors Responding to Hurricanes Katrina and Rita (open access)

Agency Management of Contractors Responding to Hurricanes Katrina and Rita

Correspondence issued by the Government Accountability Office with an abstract that begins "In February 2006, we briefed Congress on the results of our review of various contracting issues related to the Gulf Coast hurricanes. We conducted this work under the Comptroller General's statutory authority in order to assess how three agencies--the Federal Emergency Management Agency (FEMA), the U.S. Army Corps of Engineers (USACE), and the General Services Administration (GSA)--planned for and conducted oversight of several key contracts in support of Katrina and Rita response and recovery efforts."
Date: March 15, 2006
Creator: United States. Government Accountability Office.
Object Type: Text
System: The UNT Digital Library
Federal Information Collection: A Reexamination of the Portfolio of Major Federal Household Surveys Is Needed (open access)

Federal Information Collection: A Reexamination of the Portfolio of Major Federal Household Surveys Is Needed

A letter report issued by the Government Accountability Office with an abstract that begins "Federal statistical information is used to make appropriate decisions about budgets, employment, and investments. GAO was asked to (1) describe selected characteristics of federally funded statistical or research surveys, (2) describe agencies' and Office of Management and Budget's (OMB) roles in identifying and preventing unnecessary duplication, (3) examine selected surveys to assess whether unnecessary duplication exists in areas with similar subject matter, and (4) describe selected agencies' efforts to improve the efficiency and relevance of surveys. GAO reviewed agency documents and interviewed officials. Using this information and prior GAO work, GAO identified surveys with potential unnecessary duplication."
Date: November 15, 2006
Creator: United States. Government Accountability Office.
Object Type: Report
System: The UNT Digital Library
Foster Care and Adoption Assistance: Federal Oversight Needed to Safeguard Funds and Ensure Consistent Support for States' Administrative Costs (open access)

Foster Care and Adoption Assistance: Federal Oversight Needed to Safeguard Funds and Ensure Consistent Support for States' Administrative Costs

A letter report issued by the Government Accountability Office with an abstract that begins "Policymakers have expressed concern over how costs to administer the Foster Care and Adoption Assistance programs are contributing to overall increased federal expenditures for these programs, estimated by the Congressional Budget Office to rise from about $6 billion in fiscal year 2003 to $8 billion in fiscal year 2008. The purpose of these programs is to provide financial support for the proper care of children who need placement outside their homes and find adoptive homes for children with special needs. They are authorized under Title IV-E of the Social Security Act and administered by the Department of Health and Human Services' Administration for Children and Families (ACF). GAO was asked to address (1) how the amounts and types of administrative costs changed from FY 2000 to FY 2004; (2) the reasons for differences in and among states in administrative spending and how these differences affect program services; and (3) whether HHS's oversight of administrative costs provides adequate controls over program spending."
Date: June 15, 2006
Creator: United States. Government Accountability Office.
Object Type: Report
System: The UNT Digital Library
Hurricane Katrina: Better Plans and Exercises Needed to Guide the Military's Response to Catastrophic Natural Disasters (open access)

Hurricane Katrina: Better Plans and Exercises Needed to Guide the Military's Response to Catastrophic Natural Disasters

A letter report issued by the Government Accountability Office with an abstract that begins "Hurricane Katrina was one of the largest natural disasters in U.S. history. Despite a large deployment of resources at all levels, many have regarded the federal response as inadequate. GAO has a body of ongoing work that covers the federal government's preparedness and response to hurricanes Katrina and Rita. Due to widespread congressional interest, this review was performed under the Comptroller General's authority. It examined (1) the extent to which pre-Katrina plans and training exercises reflected the military assistance that might be required during a catastrophic, domestic, natural disaster, (2) the military support provided in response to Katrina and factors that affected that response, and (3) the actions the military is taking to address lessons learned from Katrina and to prepare for the next catastrophe."
Date: May 15, 2006
Creator: United States. Government Accountability Office.
Object Type: Report
System: The UNT Digital Library
THz radiation as a bunch diagnostic forlaser-wakefield-accelerated electron bunches (open access)

THz radiation as a bunch diagnostic forlaser-wakefield-accelerated electron bunches

Experimental results are reported from two measurementtechniques (semiconductor switching and electro-optic sampling) thatallow temporal characterization of electron bunches produced by alaser-driven plasma-based accelerator. As femtosecond electron bunchesexit the plasma-vacuum interface, coherent transition radiation (at THzfrequencies) is emitted. Measuring the properties of this radiationallows characterization of the electron bunches. Theoretical work on theemission mechanism is represented, including a model that calculates theTHz waveform from a given bunch profile. It is found that the spectrum ofthe THz pulse is coherent up to the 200 mu m thick crystal (ZnTe)detection limit of 4 THz, which corresponds to the production of sub-50fs (root-mean-square) electron bunch structure. The measurementsdemonstrate both the shot-to-shot stability of bunch parameters that arecritical to THz emission (such as total charge and bunch length), as wellas femtosecond synchrotron between bunch, THz pulse, and laserbeam.
Date: February 15, 2006
Creator: van Tilborg, J.; Schroeder, C. B.; Filip, C. V.; Toth, Cs.; Geddes, C. G. R.; Fubiani, G. et al.
Object Type: Article
System: The UNT Digital Library
Characterization of Turbiditic Oil Reservoirs Based on Geophysical Models of their Formation (open access)

Characterization of Turbiditic Oil Reservoirs Based on Geophysical Models of their Formation

Models are developed and solved to describe the flow of and deposition from low and high concentration turbidity currents. The shallow water equations are amended to include particle transport to describe the low concentration turbidity currents. The suspension balance model is used to describe the high concentration turbidity currents. Numerical simulations are developed to solve the highly non-linear, free boundary problems associated with these models. Simpler, algebraic scaling relationships are also developed for these models. The models are successfully validated against field observations of turbidites. With these models, one can take seismic information on the shape of the turbiditic deposit and estimate the particle size, which can be used to determine the porosity and permeability.
Date: January 15, 2006
Creator: Bonnecaze, Roger
Object Type: Report
System: The UNT Digital Library
High-resolution simulations and modeling of reshocked single-mode Richtmyer-Meshkov instability. I. Comparison to experimental data and to amplitude growth model predictions (open access)

High-resolution simulations and modeling of reshocked single-mode Richtmyer-Meshkov instability. I. Comparison to experimental data and to amplitude growth model predictions

The reshocked single-mode Richtmyer-Meshkov instability is simulated in two spatial dimensions using the fifth- and ninth-order weighted essentially non-oscillatory shock-capturing method with uniform spatial resolution of 256 points per initial perturbation wavelength. The initial conditions and computational domain are modeled after the single-mode, Mach 1.21 air(acetone)/SF{sub 6} shock tube experiment of Collins and Jacobs [J. Fluid Mech. 464, 113 (2002)]. The simulation densities are shown to be in very good agreement with the corrected experimental planar laser-induced fluorescence images at selected times before reshock of the evolving interface. Analytical, semianalytical and phenomenological linear and nonlinear, impulsive, perturbation and potential flow models for single-mode Richtmyer-Meshkov unstable perturbation growth are summarized. The simulation amplitudes are shown to be in very good agreement with the experimental data and with the predictions of linear amplitude growth models for small times and with those of nonlinear amplitude growth models at later times up to the time at which the driver-based expansion in the experiment (but not present in the simulations or models) expands the layer before reshock. The qualitative and quantitative differences between the fifth- and ninth-order simulation results are discussed. Using a local and global quantitative metric, the prediction of the Zhang and Sohn …
Date: May 15, 2006
Creator: Latini, M; Schilling, O & Don, W
Object Type: Article
System: The UNT Digital Library
Biosynthesis of the Cyclotide MCoTI-II using an Engineered Intein (open access)

Biosynthesis of the Cyclotide MCoTI-II using an Engineered Intein

Cyclotides are an emerging family of naturally occurring circular mini-proteins ({approx}30-40 amino acids) characterized by six conserved Cys residues (forming 3 disulfide bridges) that create a topologically unique structure designated as a cyclic cysteine knot (CCK). The cysteine knot motif, which is embedded within the macrocylic backbone, is described as two disulfide bridges that form a ring that is penetrated by the third disulfide bridge. The cyclic backbone and CCK motif together confer cyclotides with a remarkable stability and resistance to proteolytic, chemical, and thermal degradation. Further, cyclotides are functionally diverse and display a wide range of functions including uterotonic activity, trypsin inhibition, cytotoxicity, neurotensin binding, anti-HIV, antimicrobial, and insecticidal activity. Together, these characteristics make cyclotides attractive candidates for both drug design and agricultural applications, both in their native forms and as molecular scaffolds for the incorporation of novel bioactivities. [1] The ability to manipulate production of cyclotides within biological systems is critical for mutagenesis studies, production of grafted products, and the mass production of cyclotides with novel activities. My adviser's hope is to achieve this capability by employing recombinant DNA expression techniques to generate large combinatorial libraries of cyclotides. The advantage in creating a biosynthetic library (containing {approx}10{sup 6}-10{sup …
Date: August 15, 2006
Creator: Cantor, J & Camarero, J A
Object Type: Report
System: The UNT Digital Library
Target Plate Conditions During Stochastic Boundary Operation on DIII-D (open access)

Target Plate Conditions During Stochastic Boundary Operation on DIII-D

A major concern for large tokamaks like ITER is the presence of edge localized modes (ELMs) that repeatedly send large bursts of particles and heat into the divertor plates. Operation with resonant magnetic perturbations (RMP) at the boundary of DIII=D has suppressed ELMs for values of q95 {approx} 3.7. At the target plate, the conditions during ELM suppressed operation for both high and low collisionality are observed by a set of radially distributed Langmuir probes. At high collisionality (n*{approx}1), the target plate particle flux and temperature drops by > 30% during ELM suppression. At low collisionality (n*{approx}0.1), the core density, target plate density, and target plate particle flux drop but the plate electron temperature increases after the ELMs are suppressed. The ELM-suppressed target plate heat flux is nearly the same as the heat flux between ELMs but the (5X higher) transient heat flux peaks due to ELMs are eliminated.
Date: May 15, 2006
Creator: Watkins, J; Evans, T; Moyer, R; Lasnier, C & Rudakov, D
Object Type: Article
System: The UNT Digital Library
Experimental and Numerical Studies of Separatrix Splitting and Magnetic Footprints in DIII-D (open access)

Experimental and Numerical Studies of Separatrix Splitting and Magnetic Footprints in DIII-D

A numerical field line integration code is used to study the structure of divertor footprints produced by small non-axisymmetric magnetic perturbation in the DIII-D tokamak. The numerical modeling results are compared to experimental infrared camera data which show a splitting of the divertor target plate heat flux into several distinct peaks when an n=3 magnetic perturbation from the DIII-D I-coil is applied. The heat flux splitting consistently appears when the n=3 perturbation is applied and disappears when the perturbation is removed. The magnitude of the splitting implied by the numerical modeling is a factor of 3 smaller than the splitting seen in the experimental data. These results suggest that the plasma response to the edge resonant applied n=3 magnetic perturbation produces an amplification of the vacuum magnetic footprint structure on the divertor target plates. These results may have significant implications for the ITER divertor design.
Date: May 15, 2006
Creator: Evans, T; Joseph, I; Moyer, R; Fenstermacher, M; Lasnier, C & Yan, L
Object Type: Article
System: The UNT Digital Library
The histone H3K9 methylation and RNAi pathways regulate normalnucleolar and repeated DNA organization by inhibiting formation ofextrachromosomal DNAs (open access)

The histone H3K9 methylation and RNAi pathways regulate normalnucleolar and repeated DNA organization by inhibiting formation ofextrachromosomal DNAs

In order to identify regulators of nuclear organization, Drosophila mutants in the Su(var)3-9 histone H3K9 methyltransferase, RNAi pathway components, and other regulators of heterochromatin-mediated gene silencing were examined for altered nucleoli and positioning of repeated DNAs. Animals lacking components of the H3K9 methylation and RNAi pathways contained disorganized nucleoli, ribosomal DNA (rDNA) and satellite DNAs. The levels of H3K9 dimethylation (H3K9me2) in chromatin associated with repeated DNAs decreased dramatically in Su(var)3-9 and dcr-2 (dicer-2) mutant tissues compared to wild type. We also observed a substantial increase in extrachromosomal repeated DNAs in mutant tissues. The disorganized nucleolus phenotype depends on the presence of Ligase 4 (Lig4), and ecc DNA formation is not induced by removal of cohesin. We conclude that H3K9 methylation of rDNA and satellites, maintained by Su(var)3-9, HP1, and the RNAi pathway, is necessary for the structural stability of repeated DNAs, which is mediated through suppression of non-homologous end joining (NHEJ). These results suggest a mechanism for how local chromatin structure can regulate genome stability, and the organization of chromosomal elements and nuclear organelles.
Date: June 15, 2006
Creator: Peng, Jamy C. & Karpen, Gary H.
Object Type: Article
System: The UNT Digital Library