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Reinforcement of Aluminum Castings with Dissimilar Metals (open access)

Reinforcement of Aluminum Castings with Dissimilar Metals

The project ''Reinforcement of Aluminum Casting with Dissimilar Metal'' was a Cooperative Research and Development Agreements (CRADAs) between Oak Ridge National Laboratory (ORNL) and Cummins Inc. This project, technologies have been developed to reinforce aluminum castings with steel insert. Defect-free bond between the steel insert and the aluminum casting has been consistently obtained. The push-out experiment indicated that the bond strength is higher than that of the Al-Fin method. Two patents have been granted to the project team that is comprised of Cummins Inc. and ORNL. This report contains four sections: the coating of the steel pins, the cast-in method, microstructure characterization, and the bond strength. The section of the coating of the steel pins contains coating material selection, electro-plating technique for plating Cu and Ni on steel, and diffusion bonding of the coatings to the steel. The section of cast-in method deals with factors that affecting the quality of the metallurgical bond between the coated steel and the aluminum castings. The results of microstructure characteristics of the bonding are presented in the microstructure characterization section. A push-out experiment and the results obtained using this method is described in the section of bond strength/mechanical property.
Date: January 7, 2004
Creator: Han, Q
Object Type: Report
System: The UNT Digital Library
Cherokeean Herald (Rusk, Tex.), Vol. 154, No. 46, Ed. 1 Wednesday, January 7, 2004 (open access)

Cherokeean Herald (Rusk, Tex.), Vol. 154, No. 46, Ed. 1 Wednesday, January 7, 2004

Weekly newspaper from Rusk, Texas that includes local, state and national news along with extensive advertising.
Date: January 7, 2004
Creator: Whitehead, Marie
Object Type: Newspaper
System: The Portal to Texas History
Senate Legislative Procedures: Published Sources of Information (open access)

Senate Legislative Procedures: Published Sources of Information

The Senate publishes its rules, precedents, and other related information so that Senators and their staff have convenient access to the Senate’s legislative procedures and can gauge how those procedures are likely to apply in various situations. Information about the Senate’s legislative procedures is published in four official documents. This report discusses these documents.
Date: January 7, 2004
Creator: Davis, Christopher M.
Object Type: Report
System: The UNT Digital Library
Effect of Initial Conditions on Compressible Rayleigh-Taylor Instability and Transition to Turbulence (open access)

Effect of Initial Conditions on Compressible Rayleigh-Taylor Instability and Transition to Turbulence

Perturbations on an interface driven by a strong blast wave grow in time due to a combination of Rayleigh-Taylor, Richtmyer-Meshkov, and decompression effects. In this paper, we present the first results from a computational study of such a system under drive conditions to be attainable on the National Ignition Facility. Using the multiphysics, AMR, higher order Godunov Eulerian hydrocode, Raptor, we consider the late nonlinear instability evolution for multiple amplitude and phase realizations of a variety of multimode spectral types. We show that compressibility effects preclude the emergence of a regime of self-similar instability growth independent of the initial conditions by allowing for memory of the initial conditions to be retained in the mix-width at all times. The loss of transverse spectral information is demonstrated, however, along with the existence of a quasi-self-similar regime over short time intervals. The initial conditions are shown to have a strong affect on the time to transition to the quasi-self-similar regime.
Date: January 7, 2004
Creator: Miles, A R; Edwards, M & Greenough, J A
Object Type: Article
System: The UNT Digital Library
RESULTS OF CHARACTERIZATION TESTS OF THE SURFACES OF A COMMERCIALLY CARBURIZED AUSTENITIC STAINLESS STEEL (open access)

RESULTS OF CHARACTERIZATION TESTS OF THE SURFACES OF A COMMERCIALLY CARBURIZED AUSTENITIC STAINLESS STEEL

A commercial surface carburization treatment that shows promise for hardening the surfaces of the stainless steel target vessel of the Spallation Neutron Source against cavitation erosion and pitting caused by the action of pulsed pressure waves in the liquid mercury target has been investigated. To verify promotional claims for the treatment and to uncover any factors that might be of concern for the integrity of a carburized target vessel, some characterization tests of the nature of the surface layers of carburized austenitic 316LN stainless steel were conducted. The findings support most of the claims. The carburized layer is about 35 {micro}m thick. Its indentation hardness is about five times larger than that of the substrate steel and declines rapidly with depth into the layer. The surface is distorted by the treatment, and the austenite lattice is enlarged. The corrosion resistance of the carburized layer in an acid medium is greater than that for untreated austenite. The layer is not brittle; it is plastically deformable and is quite resistant to cracking during straining. Contrary to the provider's assertations, the maximum carbon content of the layer is much less than 6-7 wt% carbon, and the carbon is not simply contained in supersaturated …
Date: January 7, 2004
Creator: Farrell, K
Object Type: Report
System: The UNT Digital Library
The Clifton Record (Clifton, Tex.), Vol. 109, No. 2, Ed. 1 Wednesday, January 7, 2004 (open access)

The Clifton Record (Clifton, Tex.), Vol. 109, No. 2, Ed. 1 Wednesday, January 7, 2004

Semi-weekly newspaper from Clifton, Texas that includes local, state, and national news along with advertising.
Date: January 7, 2004
Creator: Smith, W. Leon
Object Type: Newspaper
System: The Portal to Texas History
Seminole Sentinel (Seminole, Tex.), Vol. 95, No. 25, Ed. 1 Wednesday, January 7, 2004 (open access)

Seminole Sentinel (Seminole, Tex.), Vol. 95, No. 25, Ed. 1 Wednesday, January 7, 2004

Semi-weekly newspaper from Seminole, Texas that includes local, state, and national news along with extensive advertising.
Date: January 7, 2004
Creator: unknown
Object Type: Newspaper
System: The Portal to Texas History
WABASH RIVER INTEGRATED METHANOL AND POWER PRODUCTION FROM CLEAN COAL TECHNOLOGIES (IMPPCCT) (open access)

WABASH RIVER INTEGRATED METHANOL AND POWER PRODUCTION FROM CLEAN COAL TECHNOLOGIES (IMPPCCT)

The Wabash River Integrated Methanol and Power Production from Clean Coal Technologies (IMPPCCT) project is evaluating integrated electrical power generation and methanol production through clean coal technologies. The project is conducted by a multi-industry team lead previously by Gasification Engineering Corporation (GEC). The project is now under the leadership of ConocoPhillips Company (COP) after it acquired GEC and the E-Gas{trademark} gasification technology from Global Energy in July 2003. The Phase I of this project was supported by Air Products and Chemicals, Inc., Dow Chemical Company, Dow Corning Corporation, Methanex Corporation, and Siemens Westinghouse Power Corporation, while the Phase II is supported by Gas Technology Institute, TDA Research, Inc., and Nucon International, Inc. The two project phases planned for execution include: (1) Feasibility study and conceptual design for an integrated demonstration facility at Global Energy's existing Wabash River Energy Limited (WREL) plant in West Terre Haute, Indiana, and for a fence-line commercial embodiment plants (CEP) operated at Dow Chemical or Dow Corning chemical plant locations (2) Research, development, and testing (RD&T) to define any technology gaps or critical design and integration issues. The WREL facility was designed, constructed, and operated under a project selected and co-funded under the Round IV of …
Date: January 7, 2004
Creator: Lynch, Thomas
Object Type: Report
System: The UNT Digital Library
Transport Visualization for Studying Mass Trasnfer and Solute Transport in Permeable Media (open access)

Transport Visualization for Studying Mass Trasnfer and Solute Transport in Permeable Media

Understanding and predicting mass transfer coupled with solute transport in permeable media is central to several energy-related programs at the US Department of Energy (e.g., CO{sub 2} sequestration, nuclear waste disposal, hydrocarbon extraction, and groundwater remediation). Mass transfer is the set of processes that control movement of a chemical between mobile (advection-dominated) domains and immobile (diffusion- or sorption-dominated) domains within a permeable medium. Consequences of mass transfer on solute transport are numerous and may include (1) increased sequestration time within geologic formations; (2) reduction in average solute transport velocity by as much as several orders of magnitude; (3) long ''tails'' in concentration histories during removal of a solute from a permeable medium; (4) poor predictions of solute behavior over long time scales; and (5) changes in reaction rates due to mass transfer influences on pore-scale mixing of solutes. Our work produced four principle contributions: (1) the first comprehensive visualization of solute transport and mass transfer in heterogeneous porous media; (2) the beginnings of a theoretical framework that encompasses both macrodispersion and mass transfer within a single set of equations; (3) experimental and analytical tools necessary for understanding mixing and aqueous reaction in heterogeneous, granular porous media; (4) a clear experimental …
Date: January 7, 2004
Creator: Haggerty, Roy
Object Type: Report
System: The UNT Digital Library
Termination Report. Supply Curves for Agricultural and Forestry Greenhouse Gas Emissions and their Use in Integrated Assessments: Methodology and Case Development (open access)

Termination Report. Supply Curves for Agricultural and Forestry Greenhouse Gas Emissions and their Use in Integrated Assessments: Methodology and Case Development

OAK-B135 The results produced by this project include: (1) Development of econometrically estimated marginal abatement and associated production curves describing response of agricultural and forestry emissions/sink/offsets enhancements for use in integrated assessments. Curves were developed that reflected agricultural, and forestry production of traditional commodities, carbon and other greenhouse gas offsets and biofuels given signals of general commodity demand, and carbon and energy prices. This work was done jointly with Dr. Ronald Sands at PNNL. A paper from this is forthcoming as follows Gillig, D., B.A. McCarl, and R.D. Sands, ''Integrating Agricultural and Forestry GHG Mitigation Response into General Economy Frameworks: Developing a Family of Response Functions,'' Mitigation and Adaptation Strategies for Global Change, forthcoming, 2004. An additional effort was done involving dynamics and a second paper was prepared that is annex A to this report and is Gillig, D., and B.A. McCarl, ''Integrating Agricultural and Forestry Response to GHG Mitigation into General Economy Frameworks: Developing a Family of Response Functions using FASOM,'' 2004. (2) Integration of the non dynamic curves from (1) into in a version of the PNNL SGM integrated assessment model was done in cooperation with Dr. Ronald Sands at PNNL. The results were reported at the second …
Date: January 7, 2004
Creator: McCarl, Bruce & Gillig, Dhazn
Object Type: Report
System: The UNT Digital Library
Historical and Retrospective Survey of Monitored Natural Attenuation: A Line of Inquiry Supporting Monitored Natural Attenuation and Enhanced Passive Remediation of Chlorinated Solvents (open access)

Historical and Retrospective Survey of Monitored Natural Attenuation: A Line of Inquiry Supporting Monitored Natural Attenuation and Enhanced Passive Remediation of Chlorinated Solvents

The Department of Energy is sponsoring an initiative to facilitate efficient, effective and responsible use of Monitored Natural Attenuation (MNA) and Enhanced Passive Remediation(EPR) for chlorinated solvents. This Office of Environmental Management Alternative Project, focuses on providing scientific and policy support for MNA/EPR. A broadly representative working group of scientists supports the project along with partnerships with regulatory organizations such as the Interstate Technology Regulatory Council and the Environmental Protection Agency. The initial product of the technical working group was a summary report that articulated the conceptual approach and central scientific tenants of the project, and that identified a prioritized listing of technical targets for field research. This report documented the process in which: (1) scientific ground rules were developed, (2) lines of inquiry were identified and then critically evaluated, (3) promising applied research topics were highlighted in the various lines of inquiry, and (4) these were discussed and prioritized. The summary report will serve as a resource to guide management and decision making throughout the period of the subject MNA/EPR Alternative Project. To support and more fully document the information presented in the summary report, we are publishing a series of supplemental documents that present the full texts from …
Date: January 7, 2004
Creator: Looney, B. B.
Object Type: Report
System: The UNT Digital Library
Summary Document of Workshops for Hanford, Oak Ridge and Savannah River Site as part of the Monitored Natural Attenuation and Enhanced Passive Remediation for Chlorinated Solvents - DOE Alternative Project for Technology Acceleration (open access)

Summary Document of Workshops for Hanford, Oak Ridge and Savannah River Site as part of the Monitored Natural Attenuation and Enhanced Passive Remediation for Chlorinated Solvents - DOE Alternative Project for Technology Acceleration

This document summarizes the result of a series of meetings with regulators, stakeholders, tribal representatives, and end users. The meetings focused on providing information from the Monitored Natural Attenuation and Enhanced Passive Remediation for Chlorinated Solvents, DOE Alternative Project for Technology Acceleration and eliciting feedback on the direction of the project, its usefulness, and its applicability to issues faced by the participants. The meetings were held with the DOE and operating contractor personnel working at and stakeholders/tribal representatives associated with the Hanford, Oak Ridge and Savannah River Sites, as well as EPA regions IV and X and state regulators from Washington, Tennessee and South Carolina. These meetings were conducted over the time period beginning August 12 and culminating September 23, 2003. Approximately 120 people participated in these meetings.
Date: January 7, 2004
Creator: Vangelas, Karen M.
Object Type: Report
System: The UNT Digital Library
Historical and Retrospective Survey of Monitored Natural Attenuation: A Line of Inquiry Supporting Monitored Natural Attenuation and Enhanced Passive Remediation of Chlorinated Solvents (open access)

Historical and Retrospective Survey of Monitored Natural Attenuation: A Line of Inquiry Supporting Monitored Natural Attenuation and Enhanced Passive Remediation of Chlorinated Solvents

The Department of Energy is sponsoring an initiative to facilitate efficient, effective and responsible use of Monitored Natural Attenuation (MNA) and Enhanced Passive Remediation(EPR) for chlorinated solvents. This Office of Environmental Management Alternative Project, focuses on providing scientific and policy support for MNA/EPR. A broadly representative working group of scientists supports the project along with partnerships with regulatory organizations such as the Interstate Technology Regulatory Council and the Environmental Protection Agency. The initial product of the technical working group was a summary report that articulated the conceptual approach and central scientific tenants of the project, and that identified a prioritized listing of technical targets for field research. This report documented the process in which: (1) scientific ground rules were developed, (2) lines of inquiry were identified and then critically evaluated, (3) promising applied research topics were highlighted in the various lines of inquiry, and (4) these were discussed and prioritized. The summary report will serve as a resource to guide management and decision making throughout the period of the subject MNA/EPR Alternative Project. To support and more fully document the information presented in the summary report, we are publishing a series of supplemental documents that present the full texts from …
Date: January 7, 2004
Creator: Looney, B. B.
Object Type: Report
System: The UNT Digital Library
Impact of Zeolite Transferred from Tank 19F to Tank 18F on DWPF Vitrification of Sludge Batch 3 (open access)

Impact of Zeolite Transferred from Tank 19F to Tank 18F on DWPF Vitrification of Sludge Batch 3

The Defense Waste Processing Facility (DWPF) is planning to initiate vitrification of Sludge Batch 3 (SB3) in combination with Sludge Batch 2 (SB2) in the spring of 2004. The contents of Sludge Batch 3 will be a mixture of the heel remaining from Sludge Batch 1B, sludge from Tank 7F (containing coal, sand, and sodium oxalate), and sludge materials from Tank 18F. The sludge materials in Tank 18F contain part of a mound of zeolitic material transferred there from Tank 19F. This mound was physically broken up and transfers were made from Tank 19F to Tank 18F for vitrification into SB3. In addition, excess Pu and Am/Cm materials were transferred to Tank 51H to be processed through the DWPF as part of SB3. Additional Pu material and a Np stream from the Canyons are also planned to be added to SB3 before processing of this batch commences at DWPF. The primary objective of this task was to assess the impacts of the excess zeolite mound material in Tank 19F on the predicted glass and processing properties of interest when the zeolite becomes part of SB3. The two potential impacts of the Tank 19F zeolite mound on DWPF processing relates to …
Date: January 7, 2004
Creator: Jantzen, Carol M.; Swingle, R. F. & Smith, F. G.
Object Type: Report
System: The UNT Digital Library
Termination Report (open access)

Termination Report

The results of this project include: (1) Development of econometrically estimated marginal abatement and associated production curves describing response of agricultural and forestry emissions/sink/offsets enhancements for use in integrated assessments. Curves were developed that reflected agricultural, and forestry production of traditional commodities, carbon and other greenhouse gas offsets and biofuels given signals of general commodity demand, and carbon and energy prices. (2) Integration of the non-dynamic curves from (1) into a version of the PNNL SGM integrated assessment model was done in cooperation with Dr. Ronald Sands at PNNL. The results were reported at the second DOE conference on sequestration in the paper listed and the abstract is in Annex B of this report. (3) Alternative agricultural sequestration estimates were developed in conjunction with personnel at Colorado State University using CENTURY and analyses can operate under the use of agricultural soil carbon data from either the EPIC or CENTURY models. (4) A major effort was devoted to understanding the possible role and applicable actions from agriculture. (5) Work was done with EPA and EIA to update the biofuel data and assumptions resulting in some now emerging results showing the criticality of biofuel assumptions.
Date: January 7, 2004
Creator: Dhazngilly, Bruce McCarl and
Object Type: Report
System: The UNT Digital Library
X-ray survival characteristics and genetic analysis for nine saccharomyces deletion mutants that show altered radiation sensitivity (open access)

X-ray survival characteristics and genetic analysis for nine saccharomyces deletion mutants that show altered radiation sensitivity

The availability of a genome-wide set of Saccharomyces deletion mutants provides a chance to identify all the yeast genes involved in DNA repair. Using X-rays, we are screening these mutants to identify additional genes that show increased sensitivity to the lethal effects of ionizing radiation. For each mutant identified as sensitive, we are confirming that the sensitivity phenotype co-segregates with the deletion allele and are obtaining multipoint survival-versus-dose assays in at least two haploid and one homozygous diploid strains. We present data for deletion mutants involving the genes DOT1, MDM20, NAT3, SPT7, SPT20, GCN5, HFI1, DCC1 and VID21/EAF1, and discuss their potential roles in repair. Eight of these genes have a clear radiation-sensitive phenotype when deleted, but the ninth, GCN5, has at most a borderline phenotype. None of the deletions confer substantial sensitivity to ultra-violet radiation, although one or two may confer marginal sensitivity. The DOT1 gene is of interest because its only known function is to methylate one lysine residue in the core of the histone H3 protein. We find that histone H3 mutants (supplied by K. Struhl) in which this residue is replaced by other amino-acids are also X-ray sensitive, seeming to confirm that methylation of the lysine-79 …
Date: January 7, 2004
Creator: Game, John C.; Williamson, Marsha S. & Baccari, Clelia
Object Type: Article
System: The UNT Digital Library
Summary Document of Workshops for Hanford, Oak Ridge and Savannah River Site as part of the Monitored Natural Attenuation and Enhanced Passive Remediation for Chlorinated Solvents - DOE Alternative Project for Technology Acceleration (open access)

Summary Document of Workshops for Hanford, Oak Ridge and Savannah River Site as part of the Monitored Natural Attenuation and Enhanced Passive Remediation for Chlorinated Solvents - DOE Alternative Project for Technology Acceleration

This document summarizes the result of a series of meetings with regulators,stakeholders, tribal representatives, and end users. The meetings focused on providing information from the Monitored Natural Attenuation and Enhanced Passive Remediation for Chlorinated Solvents, DOE Alternative Project for Technology Acceleration and eliciting feedback on the direction of the project, its usefulness, and its applicability to issues faced by the participants. The meetings were held with the DOE and operating contractor personnel working at and stakeholders/tribal representatives associated with the Hanford, Oak Ridge and Savannah River Sites, as well as EPA regions IV and X and state regulators from Washington, Tennessee and South Carolina. These meetings were conducted over the time period beginning August 12 and culminating September 23, 2003. Approximately 120 people participated in these meetings.
Date: January 7, 2004
Creator: Vangelas, Karen M.
Object Type: Report
System: The UNT Digital Library
The Archer Advocate (Holliday, Tex.), Vol. 1, No. 40, Ed. 1 Wednesday, January 7, 2004 (open access)

The Archer Advocate (Holliday, Tex.), Vol. 1, No. 40, Ed. 1 Wednesday, January 7, 2004

Weekly newspaper from Holliday, Texas that includes local, state, and national news along with advertising.
Date: January 7, 2004
Creator: Thomas, John
Object Type: Newspaper
System: The Portal to Texas History
The Baytown Sun (Baytown, Tex.), Vol. 82, No. 36, Ed. 1 Wednesday, January 7, 2004 (open access)

The Baytown Sun (Baytown, Tex.), Vol. 82, No. 36, Ed. 1 Wednesday, January 7, 2004

Daily newspaper from Baytown, Texas that includes local, state, and national news along with advertising.
Date: January 7, 2004
Creator: Cash, Wanda Garner
Object Type: Newspaper
System: The Portal to Texas History
Saturation of a High Grain Single-Pass Fel. (open access)

Saturation of a High Grain Single-Pass Fel.

We study a perturbation expansion for the solution of the nonlinear one-dimensional FEL equations. We show that in the case of a monochromatic wave, the radiated intensity satisfies a scaling relation that implies, for large distance z traveled along the undulator, a change in initial value of the radiation field corresponds to a translation in z (lethargy). Analytic continuation using Pade approximates yields accurate results for the radiation field early in saturation.
Date: January 7, 2004
Creator: Krinsky, S.
Object Type: Article
System: The UNT Digital Library
High Explosive Deonation Threshold Sensitivity Due to Multiple Fragment Impacts (open access)

High Explosive Deonation Threshold Sensitivity Due to Multiple Fragment Impacts

Fragments, bullets or projectiles can initiate a detonation in a high explosive (HE). For this to happen certain critical conditions need to be exceeded. For a given explosive, these critical conditions are the projectile velocity, the projectile size and shape, and the projectile material properties. A lot of work has been done in the area of metal shaped charge jets and individual fragments impacting the HE. One major gap in understanding initiation phenomena is the effect of multiple fragment impact. This study shows that multiple fragments can lower the fragment size and the kinetic energy thresholds.
Date: January 7, 2004
Creator: Georgevich, V; Pincosy, P & Chase, J
Object Type: Article
System: The UNT Digital Library
Using Carbon-14 Isotope Tracing to Investigate Molecular Structure Effects of the Oxygenate Dibutyl Maleate on Soot Emissions from a DI Diesel Engine (open access)

Using Carbon-14 Isotope Tracing to Investigate Molecular Structure Effects of the Oxygenate Dibutyl Maleate on Soot Emissions from a DI Diesel Engine

The effect of oxygenate molecular structure on soot emissions from a DI diesel engine was examined using carbon-14 ({sup 14}C) isotope tracing. Carbon atoms in three distinct chemical structures within the diesel oxygenate dibutyl maleate (DBM) were labeled with {sup 14}C. The {sup 14}C from the labeled DBM was then detected in engine-out particulate matter (PM), in-cylinder deposits, and CO{sub 2} emissions using accelerator mass spectrometry (AMS). The results indicate that molecular structure plays an important role in determining whether a specific carbon atom either does or does not form soot. Chemical-kinetic modeling results indicate that structures that produce CO{sub 2} directly from the fuel are less effective at reducing soot than structures that produce CO before producing CO{sub 2}. Because they can follow individual carbon atoms through a real combustion process, {sup 14}C isotope tracing studies help strengthen the connection between actual engine emissions and chemical-kinetic models of combustion and soot formation/oxidation processes.
Date: January 7, 2004
Creator: Buchholz, B. A.; Mueller, C. J.; Upatnieks, A.; Martin, G. C.; Pitz, W. J. & Westbrook, C. K.
Object Type: Article
System: The UNT Digital Library
Laser Performance Operations Model (LPOM) (open access)

Laser Performance Operations Model (LPOM)

The Laser Performance Operations Model (LPOM) has been developed to provide real time predictive capabilities for the National Ignition Facility (NIF) at Lawrence Livermore National Laboratory. LPOM uses diagnostic feedback from previous NIF shots to maintain accurate energetics models for each of the 192 NIF beamlines (utilizing one CPU per laser beamline). This model is used to determine the system setpoints (initial power, waveplate attenuations, laser diagnostic settings) required for all requested NIF shots. In addition, LPOM employs optical damage models to minimize the probability that a proposed shot may damage the system. LPOM provides post-shot diagnostic reporting to support the NIF community. LPOM was deployed prior to the first main laser shots in NIF, and has since been used to set up every shot in NIF's first quad (four beamlines). Real-time adjustments of the codes energetics parameters allows the LPOM to predict total energies within 5%, and provide energy balance within the four beamlines to within 2% for shots varying from 0.5 to 26 kJ (1.053 {micro}m) per beamline. The LPOM has been a crucial tool in the commissioning of the first quad of NIF.
Date: January 7, 2004
Creator: Shaw, M; Williams, W; House, R & Haynam, C
Object Type: Article
System: The UNT Digital Library
Assessment of Renal Ischemia By Optical Spectroscopy (open access)

Assessment of Renal Ischemia By Optical Spectroscopy

Introduction: No reliable method currently exists for quantifying the degree of warm ischemia in kidney grafts prior to transplantation. We describe a method for evaluating pretransplant warm ischemia time using optical spectroscopic methods. Methods: Lewis rat kidney vascular pedicles were clamped unilaterally in vivo for 0, 5, 10, 20, 30, 60, 90 or 120 minutes; 8 animals were studied at each time point. Injured and contra-lateral control kidneys were then flushed with Euro-Collins solution, resected and placed on ice. 335 nm excitation autofluorescence as well as cross polarized light scattering images were taken of each injured and control kidney using filters of various wavelengths. The intensity ratio of the injured to normal kidneys was compared to ischemia time. Results: Autofluorescence intensity ratios through a 450 nm filter and light scattering intensity ratios through an 800 nm filter both decreased significantly with increasing ischemia time (p < 0.0001 for each method, one-way ANOVA). All adjacent and non-adjacent time points between 0 and 90 minutes were distinguishable using one of these two modalities by Fisher's PLSD. Conclusions: Optical spectroscopic methods can accurately quantify warm ischemia time in kidneys that have been subsequently hypothermically preserved. Further studies are needed to correlate results with …
Date: January 7, 2004
Creator: Fitzgerald, J T; Demos, S; Michalopoulou, A; Pierce, J L & Troppmann, C
Object Type: Article
System: The UNT Digital Library