Natural transformation is the process by which cells take up DNA from the surrounding medium under physiological conditions, altering the genotype in a heritable fashion. This occurs without chemical or physical treatment of the cells. Certain Acinetobacter strains exhibit a strong tendency to incorporate homologous DNA into their chromosomes by natural transformation. Transformation in Acinetobacter exhibits several unique properties that indicate this system's superiority as a model for transformation studies or studies which benefit from the use of transformation as an experimental method of gene manipulation. Pseudomonas putida is the natural host of TOL plasmids, ranging between 50 kbp and 300 kbp in size and encoding genes for the catabolism of toluene, meta-toluate, and xylene. These very large, single-copy plasmids are difficult to isolate, manipulate, or modify in vitro. In this study, the TOL plasmid pDKR1 was introduced into Acinetobacter calcoaceticus strains and genetically engineered utilizing natural transformation as part of the process. Following engineering by transformation, the recombinant DNA molecule was returned to the native genetic background of the original host P. putida strain. Specific parameters for the successful manipulation of large plasmids by natural transformation in Acinetobacter were identified and are outlined. The effects of growth phase, total …

Masculine gender role conflict (MGRC) occurs when externally-imposed male gender role expectations have a negative impact on and consequences for men. The purpose of this study was to examine how men in a homogeneous setting (i.e., a college campus) compare on MGRC and psychological well-being, based on their self-identified sexual orientation. Utilizing canonical correlation analysis, 96 heterosexual men and 102 gay men were compared on four factors of MGRC (conflict between work and family, restrictive emotionality, restrictive affectionate behavior between men, and success, power, and competition) and five factors of psychological well-being (anger, anxiety, depression, self-esteem, and attitudes toward seeking psychological help). Findings for the heterosexual men were highly consistent with previous studies on MGRC and psychological well-being in a college-age population. Findings for the gay men indicated they had more problems with MGRC and psychological well-being than college-age and older gay men surveyed in the one published study on gay men and MGRC. Gay men who were single also reported more problems with restrictive emotionality, anger, anxiety, and depression, and had lower self-esteem, than gay men who were in a relationship. Between group differences were few, with gay men reporting significantly less restrictive affectionate behavior between men than heterosexual …

Companies have invested large amounts of money on information systems development. Unfortunately, not all information systems developments are successful. Software project failure is frequent and lamentable. Surveys and statistical analysis results underscore the severity and scope of software project failure. Limited research relates software structure to information systems failures. Systematic study of failure provides insights into the causes of IS failure. More importantly, it contributes to better monitoring and control of projects and enhancing the likelihood of the success of management information systems. The underlining theories and literature that contribute to the construction of theoretical framework come from general systems theory, complexity theory, and failure studies. One hundred COBOL programs from a single company are used in the analysis. The program log clearly documents the date, time, and the reasons for changes to the programs. In this study the relationships among the variables of business requirements change, software complexity, program size and the error rate in each phase of software development life cycle are tested. Interpretations of the hypotheses testing are provided as well. The data shows that analysis error and design error occur more often than programming error. Measurement criteria need to be developed at each stage of the …

The objective of this dissertation, inspired by performance experience, was to establish the similarities in the use of recitative style in the music of Claudio Monteverdi and Giuseppe Verdi. To achieve this objective, their use of recitative style was examined through comparative analysis of four scenes from their operas: “Arianna's Lament” from L'Arianna and “Disprezzata regina” from L'incoronazione di Poppea by Monteverdi, and “Condotta ell'era in ceppi” from Il trovatore and “Judgment Scene” from Aida by Verdi. The examination of the similarities included a discussion of the following: (a) the historical influences and cultural backgrounds of the composers; (b) general similarities in their compositional approaches to recitative style; (c) comparable characteristics of the dramatic recitative style in the early Baroque monody and in Verdi's operas; (d) similarities in musical characterization and expression of affective and emotional content through stylistic musical devices; (e) similarities in the composers' approaches to vocal and acting issues with special emphasis on the problems of diction; and (f) some related performance-practice issues. A discussion of the poetic lament and the influence of its form and content on musical setting was also a part of this research. The comparative research revealed numerous similarities in the historical circumstances …

This study compared the effects from the use of aural and notated exercises as pedagogical procedures for teaching harmonic accuracy to beginning jazz improvisation students. The methods of pedagogy were identified from published pedagogic and historical sources, the results of oral and written traditions of jazz pedagogy. The performance objective was produced from a review of the related literature as a recognized and measurable characteristic of jazz improvisation. The purpose of this study was to compare measurements of harmonic accuracy, following the use of notated and aural exercises as experimental procedures of jazz improvisation pedagogy. A lesson plan, materials, curriculum and outline were developed followed by student recruitment and participation. A total of 20 student volunteers participated in the methods of pedagogy (aural or notation). Data collection consisted of a musical background questionnaire and pre and posttest performance recordings. Student recordings were evaluated by six judges using the “experimental performance evaluation measure.” Statistical analyses were conducted, including comparisons of pre to posttest effects between, and among the methods of pedagogy. Although all student participants performed mostly from notated music prior to this study, students who received the aural method of pedagogy produced greater improvement for all measurement items. While the …

Mass media such as television, video players, video games, compact disks, and the computers are commonplace in current American culture. For English as a Second Language children, television may be the only source of English in the home serving as models of grammar, syntax, story structure. An investigation was made using English as a Second Language (ESL) kindergarteners, the classroom writing center, participant-observation, teacher as researcher, and case study methodology to investigate the following questions: Do ESL kindergarten children use media in their writing? If so, how do they use media in their writing? Upon examination of the data, it was found that all these ESL children did use media in the writing process. The function and form of the media references varied from child to child. Media was a cultural context for the childrenÕs social interactions. Oral language (with and without media references) not only informed the writing for some, but also served: to initiate, participate in, and sustain social relationships with peers. Findings indicated that two case study subjects used social dialogue as a separate operation from the production of a written story. Language informed the writing but it also had a socialization function in addition to what …

Changes in income and representation in managerial occupations is explored separately for women and men among the United States' eight largest race/ethnic minority groups for each decennial census of 1960, 1970, 1980 and 1990 to determine how much change has occurred between 1960 and 1990 in race and ethnic inequality, and in gender inequality within each race/ethnic group. Insights from gender theory are applied to minority group inequality and insights from minority group theory are applied to gender inequality with some degree of success. Economic change is uneven among the groups, with the largest specific change being the movement of women into managerial jobs. A clear pattern also emerged indicating that the higher the average representation of a minority group in managerial jobs, the greater the gap between women and men. The income of all persons with income, however, did not exhibit such a clear pattern across the different groups.

John Graves's creative non-fiction has earned him respect in Texas letters as a seminal writer but scarce critical commentary of his work outside the region. Ecological criticism examines how language, culture and the land interact, providing a context in which to discuss Graves in relation to the southwestern literary tradition of J. Frank Dobie, Walter P. Webb, and Roy Bedichek, to southern pastoral in the Virgilian mode, and to American nature writing. Graves's rhetorical strategies, including his appropriation of form, his non-polemical voice, his experimentation with narrative persona, and his utilization of traditional tropes of metaphor, metonymy, and irony, establish him as a conservative and Romantic writer of place concerned with the friction between traditional agrarian values and the demands of late-twentieth-century urban/technological existence. Sequentially, Graves's three main booksGoodbye to a River (1960), Hard Scrabble (1974), and From a Limestone Ledge (1980)represent a movement from the pastoral mode of the outward journey and return to the more domestic world of georgic, from the mode of leisure and contemplation to the demands and rewards of hard work and ownership. As such they represent not only progression or maturation in the arc of the narrator's life but a desire to reconcile ideological …

Research has described and many clinicians have reported the anorectic patient as socially disconnected, having a disembodied sense of self, perfectionist expectations, and inadequate and shameful feelings. The more intense the internal war, the more food-focused and self-defeating behavior ensues, thwarting one's ability to receive value, self-acceptance, and love. Addressing the anorexia phenomenon, this study considered, from a sociological perspective, the dynamics of attachment and shame. On the basis of 4 propositions and using a multi-method, case-replication design, attachment and shame patterns for 5 restrictive and 5 normal eaters were compared, as determined by scores from the Parental Bonding Instrument, Inventory of Parental and Peer Attachment, Internalized Shame Scale, and personal interviews. Analysis was progressive, as propositions were tested by pattern-matching steps of rating, comparing, and interpreting recurring responses to self-report and interview questions. All anorectics reported a dominant mother, with whom 4 were over attached and struggled ambivalently for autonomy, and a quiet, inexpressive father, whom 4 considered frequently absent or unavailable. As compared to normal eaters, anorectics' trust and communication scores were lower for both parents and peers. Generally, anorectics showed markedly higher internalized shame. Findings indicated that nonoptimal parental bonding patterns were related to shame. The maternal …

Older adults' attitudes toward mental health services have received little research attention. Overall, older adults are thought to hold relatively negative attitudes. In this study, Analysis 1 investigated historical shifts in attitudes toward mental health services among three independent samples of older adults, separated by 14-year and 9-year intervals (1977 sample, N = 90; 1991 sample, N = 101; 2000 sample, N = 99). Analysis 2 compared two samples of older and younger adults, each separated by a 9-year interval (Older Adults: 1991 sample, N = 93; 2000 sample, N = 91 and Younger Adults: 1991 sample, N = 131; 2000 sample, N = 147). Participants completed a questionnaire containing five, internally consistent scales assessing multiple dimensions of mental health attitudes (Openness, Biases, Range of Knowledge, Breadth, Help Seeking Attitudes). Analyses suggested that the 1991 and 2000 samples of older adults had more positive attitudes than did the 1977 sample. However, a sustained trend for more positive attitudes beyond 1991 was not seen. In fact, no differences existed between 1991 and 2000 samples with exception of two. Older and younger adults together had lower Biases and Breadth scores in 2000 than in 1991. Age effects, gender effects, and interactions were …

Burrows-Wheeler compression is a three stage process in which the data is transformed with the Burrows-Wheeler Transform, then transformed with Move-To-Front, and finally encoded with an entropy coder. Move-To-Front, Transpose, and Frequency Count are some of the many algorithms used on the List Update problem. In 1985, Competitive Analysis first showed the superiority of Move-To-Front over Transpose and Frequency Count for the List Update problem with arbitrary data. Earlier studies due to Bitner assumed independent identically distributed data, and showed that while Move-To-Front adapts to a distribution faster, incurring less overwork, the asymptotic costs of Frequency Count and Transpose are less. The improvements to Burrows-Wheeler compression this work covers are increases in the amount, not speed, of compression. Best x of 2x-1 is a new family of algorithms created to improve on Move-To-Front's processing of the output of the Burrows-Wheeler Transform which is like piecewise independent identically distributed data. Other algorithms for both the middle stage of Burrows-Wheeler compression and the List Update problem for which overwork, asymptotic cost, and competitive ratios are also analyzed are several variations of Move One From Front and part of the randomized algorithm Timestamp. The Best x of 2x - 1 family includes Move-To-Front, …

This study investigated the relationship between four models of student laptop computer use and three components of teacher instructional behavior: planning, implementation of instruction, and evaluation of instruction. The four models of use: full access, dispersed, class set, and mixed, represented the numerous ways teachers in public and private schools and school districts nationwide implemented student use of laptop computers. Teacher planning behavior was investigated with regard to time, frequency, complexity, difficulty, the need for revision, and use of technological resources and materials. Implementation of instruction was examined with regard to student grouping, instructional strategies, instructional content/subject matter, teacher and student roles, assignments and learning tasks, and instructional activities. The evaluation of instruction component was examined with regard to assessment tasks, grading, and assessment of homework. Using a researcher-designed questionnaire, data was gathered in a single-stage cross-sectional survey from 356 teachers working in 74 public and private schools nationwide. Results indicated models of student laptop computer use had differential effects on teacher instructional behaviors. On average, teachers found planning to be more arduous, but more collegial, especially in the mixed model. The full access and mixed models were more likely to advance a constructivist approach to teacher instructional behaviors with …

Learning communities have emerged as an efficient and effective paradigm for improving undergraduate education, especially for entering freshmen. The academy has become increasingly interested in learning outcomes and student retention, especially as they are related to the assessment of various approaches to educating the whole student. Learning community pedagogy has developed through rigorous research. However, little is known about the impact of this pedagogy upon college students' cognitive development and writing aptitude. Cognitive development theory has been most significantly influenced by the work of William G. Perry, Jr. Though no theory exists which would address the stages of writing development in university students, many composition theorists suggest a correlation between cognitive development and writing aptitude. This study measured cognitive development and writing aptitude in learning community students and non-learning community students, matching them for SAT scores, high school grade point averages, gender, and ethnicity. The research questions of interest were: 1) How does participation in a learning community affect students' cognitive development; and 2) How does participation in a learning community affect students' writing aptitude? The participants were pre- and post-assessed for cognitive development, using the Measure of Intellectual Development (MID). Additionally, participants were preand post-assessed for writing aptitude, using …

The purpose of this study was to investigate whether implementing Success For Life in Thailand would meet the needs of Thai public policy, the Thai educational system, and Thai culture. There were 46 respondents, including 4 early childhood professionals, 4 preschool owners, 6 directors, and 32 teachers. All respondents received the Success For Life training workshop. Each participant was requested to complete a questionnaire on their understanding and awareness of brain development and function, thoughts about implementing Success For Life in Thailand, and the appropriateness of Success For Life for the Thai educational system, Thai public policy, and Thai culture. In addition, all of the 4 early childhood professionals, 4 preschool owners, and 6 directors, and 8 teachers were interviewed to expand the information provided in the questionnaires. Two preschools implemented Success For Life in November 2000. Another 6 preschools implemented Success For Life in June 2001. Participating teachers in the preschools where Success For Life was implemented in November 2000 were also asked to write bimonthly journals. Journal entries included information about how participants changed their teaching styles after receiving the Success For Life training. Research findings indicated that Success For Life was appropriate to the preschool level in …

George Eliot's fiction is filled with mentoring relationships which generally consist of a wise male mentor and a foolish, egotistic female mentee. The mentoring narratives relate the conversion of the mentee from narcissism to selfless devotion to the community. By retaining the Christian value of self-abnegation and the Christian tendency to devalue nature, Eliot, nominally a secular humanist who abandoned Christianity, reveals herself still to be a covert Christian. In Chapter 1 I introduce the moral mentoring theme and provide background material. Chapter 2 consists of an examination of Felix Holt, which clearly displays Eliot's crucial dichotomy: the moral is superior to the natural. In Chapter 3 I present a Freudian analysis of Gwendolen Harleth, the mentee most fully developed. In Chapter 4 I examine two early mentees, who differ from later mentees primarily in that they are not egotists and can be treated with sympathy. Chapter 5 covers three gender-modified relationships. These relationships show contrasting views of nature: in the Dinah Morris-Hetty Sorrel narrative, like most of the others, Eliot privileges the transcendence of nature. The other two, Mary Garth-Fred Vincy and Dolly Winthrop-Silas Marner, are exceptions as Eliot portrays in them a Wordsworthian reconciliation with nature. In Chapter …

Semantic clustering has been used as a measure of learning strategies in a number of clinical populations and has been found to be deficient in individuals with Schizophrenia, but less attention has been paid to the dynamic use of this strategy over the course of fixed-order learning trials. In the current study, we examined this pattern of clustering use over trials in a sample of individuals with Schizophrenia, and explored whether the addition of this dynamic information would help us to better predict specific executive deficits. Results suggested that a decrease in semantic clustering across trials was associated with some executive deficits in the predicted manner. Nonetheless, the overall semantic clustering index generally proved more effective for the purposes, suggesting that in this population, the addition of dynamic information in strategy use is not likely to add considerably to clinical prediction and understanding.

This study involved non-experimental research to identify alumni perceptions of the strengths and weaknesses of the Doctor of Ministry degree program at Dallas Theological Seminary. An international survey was conducted to collect data from 165 Doctor of Ministry degree holders from Dallas Theological Seminary; 131 usable questionnaires were returned. A response rate of 79.4 percent was achieved. The intent of the study was to ascertain (a) the extent to which D.Min. alumni perceive that the objectives and goals of Doctor of Ministry programs at Dallas Theological Seminary are being met, (b) alumni-perceived strengths of Doctor of Ministry programs at Dallas Theological Seminary, (c) alumni-perceived weaknesses of Doctor of Ministry programs at Dallas Theological Seminary, (d) compare the findings of this case study assessment with a 1987 national study of Doctor of Ministry programs, and (e) make recommendations for the improvement of D. Min programs at Dallas Theological Seminary. The pattern that emerged from the data indicates that the D.Min. alumni believe objectives and goals of the Doctor of Ministry program at Dallas Theological Seminary are being met. In the opinion of the alumni, Doctor of Ministry programs at Dallas Theological Seminary has its strengths. The overall opinion of the D.Min. …

David Werner Amram III, born in Philadelphia in 1930 is a celebrated American composer whose works have increasingly gained worldwide attention. His compositions embrace many genres including incidental music, film scores, symphonies, concertos, sonatas, instrumental trios, quartets, cantatas and operas as well as songs. One of Amram's earliest published songs, Pull My Daisy, is from his musical score for the experimental film of the same name. The song, text by Allen Ginsberg, Neal Cassady and Jack Kerouac, is set in a jazz style. Twelve of his songs, published in three collections are drawn either from his incidental music for Shakespeare plays or from his chamber opera, Twelfth Night. Another group written for baritone voice, wind and string quintets is entitled Three Songs for America. Trail of Beauty for mezzosoprano, oboe and orchestra contains four settings of Native American texts. The first chapter of this paper provides a biography of the composer. Succeeding chapters give some analysis of representative songs from each published group, background to their composition, texts, information from reviews where available, and the composers own comments from telephone interviews with the writer. An appendix contains brief illustrations of music from representative songs. It is observed that Amram's multifaceted …

The overall purpose of this study was to examine how Certified Public Accountants (CPAs) perceive the potential use of assurance services to assess quality in accounting education programs. Survey questionnaires were mailed to a random sample of 250 CPAs in the north central Texas area. The questionnaire was designed to obtain demographic information and information relating to the respondents' perceptions of quality assessment of accounting education programs. An analysis of the results of this study suggest the following: CPAs consider (1) certain established criteria, such as SAT scores and faculty-to-student ratios, as effective measures for assessing quality attributes in accounting education programs and (2) traditional measures currently used for quality assessment in accounting education programs as only moderately effective by CPAs. CPAs are apparently seeking increased involvement with accounting education quality assessment and formulation of educational standards. They view the potential application of assurance services to accounting education quality as a way to offer a wider range of services to the public. CPAs perceive assurance services as a type of quality assessment that can be used to complement, but not replace, some of the more effective traditional methods, and as a way of enhancing the quality assessment process for accounting …

Theories in the international political economy literature, economic liberalism and dependency, are explored in order to test the effect of U.S. aid, trade, and investment on human rights conditions in recipient states. Two measures of human rights conditions serve as dependent variables: security rights and subsistence rights. The data cover approximately 140 countries from 1976-1996. Pooled cross-sectional time series analysis, utilizing ordinary least squares (OLS) with panel corrected standard errors, is employed due to the temporal and spatial characteristics of the data. The results indicate that foreign assistance and economic policy may not be the best approaches to altering poor human rights practices in the area of security rights. Economic and military aid is negatively associated with levels of security rights, supporting the traditional dependency perspective. While the results from trade and investment are generally in the positive direction, the lack of consistent statistical evidence suggests that increased trade and investment relationships do not dramatically improve security rights. We can conclude, however, that trade and investment fail to have the negative effect on security rights in less developed countries which critics of globalization suggest. Economic aid has a statistically significant negative effect on subsistence rights, while military aid seems to …

The relationship between an individual's level of spirituality, health locus of control, and participating in wellness activity was investigated. The relationship between spirituality, health locus of control on physical health was also investigated. The research question was based on prior studies that reported people who are more spiritual are healthier. Does their spirituality lead to increased levels of health, or are individual's who are more spiritual more likely to proactively take control of their health and engage in health promoting behaviors? One hundred and fifteen male and female employees completed The Spiritual Involvement and Beliefs Scale (SIBS), a spirituality measure, The Multidimensional Health Locus of Control Scale, a measure of locus of control related to health and healthcare, and The Center for Disease Control's (CDC) Health Risk Appraisal, a self-report measure of participation in health behaviors. Physical measures of health were obtained by obtaining Body Mass Index, blood pressure readings, and a cholesterol screening. The current study looked at level of spirituality (internal, external), level of health locus of control (internal, powerful other, chance) and participation in wellness/health promoting behaviors and health. Correlational analyses were performed on the relationship between spirituality and health locus of control. Hierarchical multiple regressions were …

By the year 2000 women will constitute more than 50 percent of the workforce in the United States, yet their representation in top management and executive-level positions continues to hover in the single digits. This “glass ceiling,” which is conceptualized as limiting women's advancement into these roles, has been the subject of much debate and research over the last fifteen years. As both an equal rights and key competitive issue, the topic of women and leadership is gaining ever-increasing emphasis and momentum in American corporations. Although leadership skills have been advocated as a key human capital/person-centered variable leading to managerial ascendancy for women, the empirical research directly investigating this link is virtually non-existent. This longitudinal study proposed to measure the strength of this relationship using a matched sample of male and female managers. Eighty-five subjects, from the same U.S. based health-care products corporation, had previously participated in a multirater assessment process where seven different facets of their leadership skills were evaluated. Time two data were collected on four objective measures of ascendancy: percent change in salary, number of promotions (job moves) either offered or accepted, change in number of direct reports, and change in number of indirect reports. Multivariate analysis …

Recently, plastidial carbonic anhydrase (CA, EC 4.2.1.1) cDNA clones encoding functional CA enzymes were isolated from a nonphotosynthetic cotton tissue. The role of CA in photosynthetic tissues have been well characterized, however there is almost no information for the role of CA in nonphotosynthetic tissues. A survey of relative CA transcript abundance and enzyme activity in different cotton organs revealed that there was substantial CA expression in cotyledons of seedlings and embryos, both nonphotosynthetic tissues. To gain insight into the role(s) of CA, I examined CA expression in cotyledons of seedlings during post-germinative growth at different environmental conditions. CA expression in cotyledons of seedlings increased from 18 h to 72 h after germination in the dark. Seedlings exposed to light had about a 2-fold increase in CA activities when compared with seedlings kept in the dark, whereas relative CA transcript levels were essentially the same. Manipulation of external CO2 environments [zero, ambient (350 ppm), or high (1000 ppm)] modulated coordinately the relative transcript abundance of CA (and rbcS) in cotyledons, but did not affect enzyme activities. On the other hand, regardless of the external CO2 conditions seedlings exposed to light exhibited increase CA activity, concomitant with Rubisco activity and increased …

This study was designed to investigate the effectiveness of a filial therapy training model with high school students enrolled in a Peer Assistance Leadership (PAL) program. Specifically, this study was designed to determine the effectiveness of filial therapy in: (1) increasing observed empathic behavior with children, (2) increasing acceptance toward children, (3) increasing the ability to allow children self-direction, and (4) increasing the level of involvement with children. Additionally, this study was designed to determine the effectiveness of filial therapy in facilitating healthy parenting attitudes of nonparenting adolescents. A research question was presented to determine if a relationship exists between empathy, acceptance, involvement and allowing children self-direction and other factors considered to be healthy parenting attitudes. An Analysis of Covariance on post-test scores revealed significant findings in the high school students ability to demonstrate empathy towards children, allowing the child self direction, communication of acceptance, and involvement as measured by the Measurement of Empathy in Adult-Child Interactions (MEACI). An Analysis of Covariance on post-test scores revealed no significant changes in parenting attitudes as measured by Adult- Adolescent Parenting Inventory (AAPI-2). A Bivariate Correlation revealed a statistically significant correlation between the Empathy, Acceptance, Allowing the Child Self-Direction and Involvement scales on …