In order to gain a better understanding of this microorganismand its role in human pathogenesis, a physical map of Moraxella catarrhalis type strain ATCC25238 was constructed using pulsed field gel electrophoresis (PFGE) in combination with Southern hybridization techniques. Restriction endonucleases Not I, Rsr II, and Sma I were used to digest the chromosomal DNA. An overlapping circular map was generated by cross-hybridization of isolated radiolabeled fragments of Moraxella catarrhalis genomic DNA to dried PFGE gels. The number and location of the 16S and 23S ribosomal RNA genes were determined by digestion with l-Ceul enzyme and by Southern hybridization. Virulence-associated genes, the gene for β-lactamase, and housekeeping genes were also placed onto the physical map.

Two years before impoundment, the trophic status of Lake Ray Roberts was predicted by applying the total phosphorus input into the Organization for Economic Cooperation and Development (OECD) eutrophication model. Predicted mean summer epilimnetic (MSE) chlorophyll a of Elm Fork arm, Isle duBois arm and Main Body were in the eutrophic category of the OECD model. Observed MSE chlorophyll a two years after impoundment of Elm Fork arm, Isle duBois and Main Body had not reached their predicted means and were at the mesotrophic-eutrophic boundary of the OECD model. Six years after impoundment, observed MSE chlorophyll a for Main Body, was closer to its predicted mean and in the eutrophic category of the OECD model. Six years after impoundment, Elm Fork arm was the most productive area of Lake Ray Roberts. Observed means of chlorophyll a, total phosphates, suspended solids and turbidity were often highest in the Elm Fork arm. Wastewater effluent from Gainesville and Valley View, TX, had an impact on productivity in Elm Fork arm.

Plasma cleaning is the most effective dry process to remove surface contaminates from a SAW (Surface Acoustical Wave) device. Consistent gas pressures, flows, and good electrical connections between the chamber shelves are necessary for the process to function predictably. In addition, operation of the monitoring system must be transparent to the plasma cleaning unit. This thesis describes a simple solution to the complex problem of monitoring a plasma cleaning system. The monitoring system uses the LabVIEW® G programming language and hardware, both products of National Instruments, Inc.®, to monitor critical parameters necessary to achieve a consistent process when cleaning these devices.

Both Chaucer and the Gawain-poet use the Matters of Troy and Thebes as material for a critique of courtly life, applying these literary matters to the events and actions in and around Ricardian England. They use these classical matters to express concerns about the effectiveness of the court of Richard II. Chaucer uses his earlier works as a testing ground to develop his views about the value of duty over courtly pursuits, ideas discussed more completely in Troilus and Criseyde. The Gawain-poet uses the Matter of Troy coupled with the court of King Arthur to engage in a critique of courtly concerns. The critiques presented by both poets show a tendency toward duty over courtly concerns.

Little scholarship has been dedicated to nineteenth-century Texas family life and no published scholarship to date has addressed the more specific topic of divorce. This study attempts to fill that gap in the historiography through a quantitative analysis of 373 divorce actions filed in Washington and Harrison Counties. The findings show a high degree of equity between men and women in court decisions granting divorces, and in property division and custody rulings. Texas women enjoyed a relatively high degree of legal and personal autonomy, which can be attributed, in part, to a property-rights heritage from Spanish civil law.

The opera is scored for chamber orchestra consisting of one oboe, two Bb clarinets, two horns in F, one trumpet in C, one tenor trombone, two percussionists (playing snare drum, bass drum, tambourine, wood block, triangle, suspended cymbal, crash cymbal, agogo bells, cow bell, brake drum, metal whistle, whip, large gong, Glockenspiel, chimes, timpani in F (low) and C), eight or more violins in two parts, six or more violas in two parts, and eight or more cellos in two parts. The characters are Alejandro Jiminez, a dramatic tenor; the Manager of Burger Palace, a baritone; the Suits 1/Fast Food Workers, a choir (SATB) and the Suits 2/Customers, a second choir (SATB), each ideally consisting of eight vocalists for a total of sixteen; the Daydream Figures, which are mimed parts; the Man with Gun, which is a spoken part. The opera, in one act consisting of six scenes and an interlude, is based on a libretto by the composer. There is only one scene change: from an essentially empty stage to a fast food restaurant in Scene 4. The length of the work is approximately sixty to sixty-five minutes.

The purpose of the study was to determine (a) types of marketing strategies, (b) perceived success of marketing strategies, (c) importance of marketing strategies, (d) amenities offered to consumers, and (e) negatives (problems) in marketing strategies by bed and breakfast owners/operators. A questionnaire to assess areas of concern covered in the research questions was developed. The questionnaire contained closed- and open-ended questions, with nominal, interval, and ratio levels of measurement. The sampling frame included the 1,140 bed and breakfast owners/operators listed in the Texas Hotel Occupancy Tax Accounts Report, first quarter, 1998, as interpreted for this study. A sample of 775 of this population was randomly chosen, using a random numbers table. A response rate of 36% was obtained.

The intent of this study was to determine what understanding and knowledge parents had of developmentally appropriate practice (DAP). The study examined whether the beliefs of parents who enrolled their children in a National Association for the Education of Young Children (NAEYC) accredited program had any impact on their expectations for a philosophy and curriculum that is centered around DAP. In addition, the study examined whether parents' understanding of DAP changed when their children transitioned from infant and toddler programs, to preschool. The study group consisted of parents with children in two privately owned NAEYC accredited centers in 1998 (N=131). Results from parent reports indicated a high level of parent knowledge regarding DAP.

This thesis is a creative work that is segmented into three main phases in order to display the developing poetic growth and control in the work of Paul Andrew Thies. The first phase is titled "Skeletons and Rhinoceri." It was a phase where I focused on more classical forms of poetry, namely accentual and syllabical sonnets. This phase was greatly influenced by both Charles Baudelaire and William Butler Yeats. The second phase, titled "Clandestinies," was one in which I tried to develop a more dense form. Lord Byron and Pablo Neruda were the two main influences on my work at this time, largely in terms of imaginative exoticism and figurative energy. The third section of this thesis, titled "Graffiti in the Orchard," is an exploration of my current work as a poet. In this phase, Rainer Maria Rilke was the primary influence as I began to develop a more fluid and expressive style.

Studies addressing racial/ethnic identity development have often overlooked the developmental cultural context. The impact of growing up with contradictory cultures has not been well explored. Immersion in multiple cultures may produce mixed patterns of strengths deficits. This study reviews the literature's currently inconsistent usage of the terms race, ethnicity, and culture; introduces the concept and theoretical framework of Cultural Homelessness; relates CH to multicultural integration; and develops two study-specific measures (included) to examine the construct validity of CH. The sample’s (N = 448, 67% women) racial, ethnic, and cultural mixture was coded back three generations using complex coding criteria. Empirical findings supported the CH-specific pattern of cognitive and social strengths with emotional difficulties: social adaptability and cross-cultural competence but also low self-esteem and shame regarding diff

The 1990s were characterized by severe turbulence in the global economy. Economic and financial crises occurred in Latin America, Middle and Eastern Europe and Asia. This analysis distinguishes between the two socioeconomic criteria "transitional" and "emerging" region. Transitional countries are former centrally planned socialist economies and emerging countries former agricultural-oriented classical developing economies with mostly a history of military or some other kind of autocratic dictatorship. The resources for the analysis are data sets regarding investment, exchange rate behavior, government finance, international liabilities of monetary authorities and inflation. The study reveals macroeconomic patterns associated with economic development in each socioeconomic region. It is shown that similar patterns are responsible for successful and non-successful performance in each region. A comparison of different regions shows many parallels between emerging economies, but only little similarity between transitional economies.

This study describes the development and validation of mutagenized cloned DNA constructs, which correspond to the polymorphic regions of the class II region of the HLA complex. The constructs were used to verify the allelic specificity of primers and probes in polymerase chain reaction (PCR)-based HLA typing assays such as Sequence Specific Primers (SSP) and Sequence Specific Oligonucleotide Probes (SSOP). The constructs consisted of the entire polymorphic region of exon 2 of class II HLA allele sequences that included primer annealing sites or probe hybridization sites. An HLA allele sequence was inserted into a plasmid, cloned, then mutagenized to match a specific HLA allele, and finally, the correct clone was verified by bidirectional sequencing of the insert. Thus, the construct created a cloned reference DNA sample for any specific allele, and can be used to validate the accuracy of various molecular methodologies.

Twi major areas concerning the problems of practice are discussed. One is that poor practice often relegates itself to mindless repetition. The second problem is that the student often has a vague definition of piano technique. All technique should be a means of expression, not just an isolated physical exercise. Therefore, the purpose of this thesis was to understand the nature of practice and to develop a suggested practice routine which incorporates both technical and musical aspects. Two recommendations, strategies toward effective practice and an ideal practice sessions, serve as a practice outline and reference for both piano teacher and student. An appendix presents a collection of the thoughts and viewpoints on practicing from forty-four internationally acclaimed pianists.

This study conducts watershed analysis using biological and geo-spatial techniques. Incorporating landscape features with biological attributes has been shown to be an effective method of monitoring environmental quality within watersheds. In situ biomonitoring using the Asiatic Clam, Corbicula fluminea, habitat suitability, and water quality data were evaluated for their potential to describe ecological conditions in agricultural and urban areas within the Upper Trinity River watershed. These data were analyzed with GIS to identify effects of land use on ecological conditions. C. fluminea downstream of point source effluents was effective detecting in-stream toxicity. Ambient toxicity appears to have improved in the Trinity, although urban influences limit aspects of aquatic life. No association between habitat quality and land use was identified.

This thesis examines access to managerial jobs in the Australian labor market by immigrant women and men from five continents and five individual countries. Comparisons were not made only among both continent and country groups, but also between the women and men within each group, as a measure of occupational gender inequality. An index of managerial representation in the Australian labor market (MORI) was computed and nine independent variables were applied to measure immigrant representation in managerial occupations. Rank order correlates were used to calculate relationships between variables. Results indicate that women (with the exception of Vietnamese) from all countries were disproportionately underrepresented in managerial jobs and that the more dissimilar immigrant men are to native born Australians, the less likely they are to hold managerial jobs.

I intend to explore various canvas sizes and many formal issues, focusing on space and the application of paint are paramount in my work. I used closely cropped photographs to aid in this study and my exploration addressed the following questions: 1) How does the speed of execution affect the spontaneity and authority of the piece? 2) How important is the unexpected for the success of my painting in regards to formal issues?

a. How does the use of different materials such as string, screen, fabric or wire personalize the work? b. How does the addition of subtly recognizable imagery change the reductive field? c. How do processes such as wrapping, printing, dripping, and other techniques infer meaning? d. How does the choice of color and scale affect the work?

My interest in the patterns of nature and culture has led me on a long journey through studies of math, sciences, and religions culminating in my current studies in art. Continuing my inquiry into processes of human perception and conception of time. My intention was to incorporate the "process" aspect of the printmaking medium into the final work by creating hybrid works that would take the "print" out of its traditional two-dimensional form. Finally, I planned to utilize the "multiple" aspect of printmaking to address ideas of patterns and evolution.

My premise was the marriage of two forms, book and sculpture. My goal was to determine if these two unique types of work could be joined as one. In each oth the proposed works, I focused on an element of design as well as the challenges present when works are produced for a particular space (the Cora Stafford Gallery).

In the study of team-based organizations most of the research has focus on the internal make-up and structure of teams. Recently there has been more interest in the effects that environment has on teams. With this new focus Support Systems in organizations have become an area of interest. Examining the perceptions of workers with respect to support systems of organizations could give insight into performance. This study specifically examines the interaction between a team's shared perception of the support in their environment and the level of support in their environment. The interaction between the two does seem to have a strong relationship with perceived performance. How do the two concepts interact, and what does this mean for organizational designers? Both questions are discussed.

Hygrophila polysperma is a plant native to Asia that has been introduced into the Comal River, TX and is thriving while Ludwigia repens, a species native to the river appears to be declining. Both plants have similar morphologies and occupy similar habitats in the river. Two plant competition experiments were conducted to examine the competitive interactions between the two species. First, an experimental design was developed in which established Ludwigia plants were 'invaded' by sprigs of Hygrophila to determine if established Ludwigia populations would be negatively impacted by invasion. The second experiment focused on establishment and growth of sprigs of each species under three competition scenarios. Results show that the continued growth of well-established Ludwigia plants was significantly depressed by the invasion of Hygrophila in comparison with those that had not been invaded. Furthermore, the growth of Hygrophila sprigs was uninhibited by the presence of Ludwigia, but the presence of Hygrophila negatively impacted the growth of Ludwigia sprigs. There was no difference in the growth of Hygrophila sprigs whether planted alone, with Ludwigia sprigs or even if planted into stands of established Ludwigia.

An event-driven, sequential, process control system was designed for International Isotopes, Inc., to automate and remotely control a target station at the company's linear accelerator facility. The designed system consisted of two major sections: a software program (virtual instrument), which was developed by LabVIEW, and a hardware interface (FieldPoint Modular Distributed I/O System by National Instrument), which had to be a pre-developed system that did not require customization. The designed virtual instrument was tested on a simulation model that mimed the target station. The result was a valid design.

Adolescent females are committing an increasing number of violent interpersonal acts. This study used the qualitative technique of focus group interviews to explore social control and self-control factors which impact such behaviors. Forty-seven girls aged 10-18 years described situations and events where interpersonal violence might be used and also what might prevent such acts. For the girls interviewed, social controls were the predominant means of controlling the use of interpersonal violence. Family and peer groups were the most powerful social controls, whether positive or negative. Self-control was deemed important but most girls lacked either the skills or desire to engage in self-control. Violence prevention programs need to teach techniques for improving self-control and increasing self-concept to be most effective.

The purpose of the study was to design and validate an instrument designed to investigate teachers' attitudes in regard to adolescent sexuality education. Test development adhered to the guidelines of Aligna and Crocker. Cronbach alpha was performed on the entire instrument and determined the reliability to be .8003, thus the instrument was consistent in measuring the domain areas. A panel of experts assessed content validity of items. A principal component factor analysis and a Pearson's product moment correlation were used for construct validity. Teacher concerns about curriculum implementation, teacher comfort with the subject matter, course-specific teacher attitudes, teacher interest about curriculum content, and teacher attitudes toward sexuality were found to be the constructs of the TACTS.