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Challenges in biotechnology at LLNL: from genes to proteins
This effort has undertaken the task of developing a link between the genomics, DNA repair and structural biology efforts within the Biology and Biotechnology Research Program at LLNL. Through the advent of the I.M.A.G.E. (Integrated Molecular Analysis of Genomes and their Expression) Consortium, a world-wide effort to catalog the largest public collection of genes, accepted and maintained within BBRP, it is now possible to systematically express the protein complement of these to further elucidate novel gene function and structure. The work has ensued in four phases, outlined as follows: (1) Gene and System selection; (2) Protein expression and purification; (3) Structural analysis; and (4) biological integration. Proteins to be expressed have been those of high programmatic interest. This includes, in particular, proteins involved in the maintenance of genome integrity, particularly those involved in the repair of DNA damage, including ERCC1, ERCC4, XRCC2, XRCC3, XRCC9, HEX1, APN1, p53, RAD51B, RAD51C, and RAD51. Full-length cDNA cognates of selected genes were isolated, and cloned into baculovirus-based expression vectors. The baculoviral expression system for protein over-expression is now well-established in the Albala laboratory. Procedures have been successfully optimized for full-length cDNA clining into expression vectors for protein expression from recombinant constructs. This includes the …
Date:
March 11, 1999
Creator:
Albala, J S
Object Type:
Report
System:
The UNT Digital Library
Physical Basis for Optical Performance of the NIF Amplifiers
None
Date:
January 11, 1999
Creator:
Alger, T.; Erlandson, A.; Fulkerson, S.; Horvath, J.; Jancaitis, K.; Marshall, C. et al.
Object Type:
Report
System:
The UNT Digital Library
The Oklahoma Daily (Norman, Okla.), Vol. 83, No. 81, Ed. 1 Monday, January 11, 1999
Student newspaper of the University of Oklahoma in Norman, Oklahoma that includes national, local, and campus news along with advertising.
Date:
January 11, 1999
Creator:
Allam, Heather
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
The Oklahoma Daily (Norman, Okla.), Vol. 83, No. 103, Ed. 1 Thursday, February 11, 1999
Student newspaper of the University of Oklahoma in Norman, Oklahoma that includes national, local, and campus news along with advertising.
Date:
February 11, 1999
Creator:
Allam, Heather
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
The Oklahoma Daily (Norman, Okla.), Vol. 83, No. 123, Ed. 1 Thursday, March 11, 1999
Student newspaper of the University of Oklahoma in Norman, Oklahoma that includes national, local, and campus news along with advertising.
Date:
March 11, 1999
Creator:
Allam, Heather
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
Deep Levels in p- and n-type InGaAsN for High Efficiency Multi-Junction III-V Solar Cells
Red Teaming is an advanced form of assessment that can be used to identify weaknesses in a variety of cyber systems. it is especially beneficial when the target system is still in development when designers can readily affect improvements. This paper discusses the red team analysis process and the author's experiences applying this process to five selected Information Technology Office (ITO) projects. Some detail of the overall methodology, summary results from the five projects, and lessons learned are contained within this paper.
Date:
November 11, 1999
Creator:
Allerman, Andrew A.; Jones, Eric D.; Kaplar, Robert J.; Kurtz, Steven R.; Kwon, Daewon & Ringel, Steven A.
Object Type:
Article
System:
The UNT Digital Library
Jahn-Teller splitting and Zeeman effect of acceptors in diamond.
Employing the high resolution of a 5+4 tandem Fabry-Perot interferometer, we discovered that {Delta}{prime}, the Raman active electronic transition between the spin-orbit split 1s(p{sub 3/2}): {Lambda}{sub 8} and 1s(P{sub 1/2}) {Lambda}{sub 7} acceptor ground states, is a doublet for a boron impurity in diamond with a clearly resolved spacing of 0.81 {+-} 0.15 cm{sup {minus}1}. The direct observation of a Stokes/anti-Stokes pair with 0.80 {+-} 0.04 cm{sup {minus}1} shift provides a striking confirmation that the lower 1s(p{sub 3/2}): {Lambda}{sub 8} ground state has experienced a splitting due to a static Jahn-Teller distortion. The Zeeman effect of {Delta}{prime} has been investigated with a magnetic field along several crystallographic directions. Theory of the Zeeman effect, formulated in terms of the symmetry of the substitutional acceptor and the Luttinger parameters of the valence band, allows quantitative predictions of the relative intensities of the Zeeman components in full agreement with experiments. The observation of transitions within the {Lambda}{sub 8} Zeeman multiplet, i.e., the Raman-electron-paramagnetic-resonances, is yet another novel feature to emerge from the present study. The investigation has also yielded g-factors characterizing the Zeeman multiplets.
Date:
September 11, 1999
Creator:
Anthony, T. R.; Grimsditch, M.; Kim, H.; Ramdas, A. K. & Rodriguez, S.
Object Type:
Article
System:
The UNT Digital Library
[News report: Gays vow to keep fighting for rights]
News report about a woman named Sue Reardon coping with the Hawaiian Supreme Court's ruling upholding current state marriage laws.
Date:
December 11, 1999
Creator:
Arakawa, Lynda
Object Type:
Text
System:
The UNT Digital Library
Benchmarking D and D procurement best practices at four commercial nuclear power plants.
The Department of Energy (DOE) has as two of its strategic objectives to safely accomplish the world's largest environmental clean-up of contaminated sites and the adoption of the best management practices of the private sector to achieve business-like results efficiently and effectively. An integral part of the strategic response to the challenges facing the Department has been the use of benchmarking and best practice management to facilitate identifying and implementing leading-edge thinking, practices, approaches, and solutions.
Date:
May 11, 1999
Creator:
Arflin, J.; Baker, G.; Bidwell, B.; Bugielski, D.; Cavanagh, J. & Sandlin, N.
Object Type:
Article
System:
The UNT Digital Library
Guthrie Historic District Restoration
Photograph of a scene during the restoration of Guthrie's Historic Downtown District.
Date:
October 11, 1999
Creator:
Argo, Jim
Object Type:
Photograph
System:
The Gateway to Oklahoma History
Guthrie Historic District Restoration
Photograph of a scene during the restoration of Guthrie's Historic Downtown District.
Date:
October 11, 1999
Creator:
Argo, Jim
Object Type:
Photograph
System:
The Gateway to Oklahoma History
Guthrie Historic District Restoration
Photograph of a scene during the restoration of Guthrie's Historic Downtown District.
Date:
October 11, 1999
Creator:
Argo, Jim
Object Type:
Photograph
System:
The Gateway to Oklahoma History
Guthrie Historic District Restoration
Photograph of a scene during the restoration of Guthrie's Historic Downtown District.
Date:
October 11, 1999
Creator:
Argo, Jim
Object Type:
Photograph
System:
The Gateway to Oklahoma History
Guthrie Historic District Restoration
Photograph of a scene during the restoration of Guthrie's Historic Downtown District.
Date:
October 11, 1999
Creator:
Argo, Jim
Object Type:
Photograph
System:
The Gateway to Oklahoma History
Guthrie Historic District Restoration
Photograph of a scene during the restoration of Guthrie's Historic Downtown District.
Date:
October 11, 1999
Creator:
Argo, Jim
Object Type:
Photograph
System:
The Gateway to Oklahoma History
Guthrie Historic District Restoration
Photograph of a scene during the restoration of Guthrie's Historic Downtown District.
Date:
October 11, 1999
Creator:
Argo, Jim
Object Type:
Photograph
System:
The Gateway to Oklahoma History
Guthrie Historic District Restoration
Photograph of a scene during the restoration of Guthrie's Historic Downtown District.
Date:
October 11, 1999
Creator:
Argo, Jim
Object Type:
Photograph
System:
The Gateway to Oklahoma History
Guthrie Historic District Restoration
Photograph of a scene during the restoration of Guthrie's Historic Downtown District.
Date:
October 11, 1999
Creator:
Argo, Jim
Object Type:
Photograph
System:
The Gateway to Oklahoma History
Guthrie Historic District Restoration
Photograph of a scene during the restoration of Guthrie's Historic Downtown District.
Date:
October 11, 1999
Creator:
Argo, Jim
Object Type:
Photograph
System:
The Gateway to Oklahoma History
Guthrie Historic District Restoration
Photograph of a scene during the restoration of Guthrie's Historic Downtown District.
Date:
October 11, 1999
Creator:
Argo, Jim
Object Type:
Photograph
System:
The Gateway to Oklahoma History
Guthrie Historic District Restoration
Photograph of a scene during the restoration of Guthrie's Historic Downtown District.
Date:
October 11, 1999
Creator:
Argo, Jim
Object Type:
Photograph
System:
The Gateway to Oklahoma History
Guthrie Historic District Restoration
Photograph of a scene during the restoration of Guthrie's Historic Downtown District.
Date:
October 11, 1999
Creator:
Argo, Jim
Object Type:
Photograph
System:
The Gateway to Oklahoma History
Guthrie Historic District Restoration
Photograph of a scene during the restoration of Guthrie's Historic Downtown District.
Date:
October 11, 1999
Creator:
Argo, Jim
Object Type:
Photograph
System:
The Gateway to Oklahoma History
Guthrie Historic District Restoration
Photograph of a scene during the restoration of Guthrie's Historic Downtown District.
Date:
October 11, 1999
Creator:
Argo, Jim
Object Type:
Photograph
System:
The Gateway to Oklahoma History