Recently, three distinct isoforms of phospholipase D (PLD) were identified in Arabidopsis thaliana. PLD α represents the well-known form found in plants, while PLD β and γ have been only recently discovered (Pappan et al., 1997b; Qin et al., 1997). These isoforms differ in substrate selectivity and cofactors required for activity. Here, I report that PLD β and γ isoforms were active toward N-acylphosphatidylethanolamine (NAPE), but PLD α was not. The ability of PLD β and γ to hydrolyze NAPE marks a key difference from PLD α. N-acylethanolamines (NAE), the hydrolytic products of NAPE by PLD β and γ, inhibited PLD α from castor bean and cabbage. Inhibition of PLD α by NAE was dose-dependent and inversely proportional to acyl chain length and degree of unsaturation. Enzyme kinetic analysis suggested non-competitive inhibition of PLD α by NAE 14:0. In addition, a 1.2-kb tobacco (Nicotiana tabacum L.) cDNA fragment was isolated that possessed a 74% amino acid identity to Arabidopsis PLD β indicating that this isoform is expressed in tobacco cells. Collectively, these results provide evidence for NAE producing PLD activities and suggest a possible regulatory role for NAE with respect to PLD α.

The purpose of this study was to determine the instructional methods of Oklahoma's elementary school music educators with respect to the inclusion of an authentic repertoire of American Indian music in the curriculum. The research was conducted through two methods. First, an analysis and review of adopted textbook series and pertinent supplemental resources on American Indian music was made. Second, a survey of K-6 grade elementary music specialists in Oklahoma during the 1997-1998 school year was conducted.

This study examined the policy letters and verbal testimony transcripts submitted by interest groups to the United States Department of Education (USDE) in response to the proposed regulations pertaining to the implementation of the 1997 reauthorization of P. L. 105-17, Individuals with Disabilities Education Act (IDEA). Specifically, this study analyzed the emerging themes in the area of discipline. Responses were received from the following interest groups: (a) school administrators, (b) parents, (c) teachers, (d) state educational agencies (SEAs), (e) national educational organizations, and (f) members of the United States Congress. In addition to analyzing the emerging themes, the study compared these themes to ones found in the current literature and court cases.

The goals of revision for the Wechsler Intelligence Scale for Children-Third Edition included enhancement of the factor structure, improvement of subtests, and revision of norms. The researchers reported that the very few items that were found to be biased were replaced. The WISC-III performance of a clinical sample of African American, Caucasian, and Hispanic children was analyzed to determine if the test bias was eliminated as claimed in the goals of the revision.

Results of a functional analysis indicated that the self-injurious behavior (SIB) of an adult female with profound mental retardation occurred primarily in the alone and demand conditions. Graphs of the separate topographies (head slaps and head bangs) showed that head banging occurred in the alone condition and that both head banging and head slapping occurred in the demand condition. A data analysis procedure to identify within-session trends across sessions and fluctuations in rates of SIB by topography revealed that most of the demands escaped were escaped by head slaps and that over 80% of all head slaps were associated with escape, compared to less than 1%of all head bangs, indicating that head banging and head slapping were members of separate functional response classes. Treatment consisted of noncontingent availability of preferred leisure materials, and produced substantial decreases of both head banging and head slapping. Interpretation of the results are discussed, as well as some implications and limitations of the study.

The purpose of this study was to assess rape attitudes and empathy levels of students at a university in North Texas. The Attitudes Toward Rape questionnaire and the Rape Empathy Scale were administered to 387 undergraduate students. Dependent variables were attitudes and empathy and independent variables were prior knowledge or experience as a rape victim, having female siblings, gender, marital status, and age. Significance was found between rape-intolerant attitudes and both prior experience as a victim (p < .001), and gender (p < .001). Significance was also found between empathy and experience as a rape victim (p < .035) and gender (p < .032).

The purpose of this study was to delineate the differences between the types of behavioral management techniques used by teachers of students with emotional/behavioral disorders.

This study examines the social and economic characteristics of the men who joined the Confederate Fourteenth Texas Infantry Regiment during the Civil War and provides a narrative history of the regiment's wartime service. The men of the Fourteenth Infantry enlisted in 1862 and helped to turn back the Federal Red River Campaign in April 1864. In creating a portrait of these men, the author used traditional historical sources (letters, diaries, medical records, secondary narratives) as well as statistical data from the 1860 United States census, military service records, and state tax rolls. The thesis places the heretofore unknown story of the Fourteenth Texas Infantry within the overall body of Civil War historiography.

The present work provides charge state fractions that may be used to generate TEAMS relative sensitivity factors for impurities in semiconductor materials.

The major purposes of the current research are twofold. The first purpose is to present a composite approach to the general classification problem by using outputs from various parametric statistical procedures and neural networks. The second purpose is to compare several parametric and neural network models on a transportation planning related classification problem and five simulated classification problems.

Carbonic anhydrase is a ubiquitous zinc-metalloenzyme that catalyzes the interconversion of carbon dioxide and carbonate and has been found to play a wide range of roles in animals, plants and bacteria. Cotton genomic and cDNA libraries were screened for the plastidial isoform of carbonic anhydrase. The nucleotide sequences of two 1.2 Kb partial cDNA clones were determined. These clones exhibit high homology to carbonic anhydrases from other dicot plants and possess all the expected peptide motifs. For example, serine and threonine rich chloroplastic targeting peptide and conserved zinc binding residues are both present. These clones were utilized to isolate two carbonic anhydrase genes that were shown to encode different isoforms by PCR and RFLP analysis.

The purposes of this study were to: (a) determine the effects of Senate Bill 7 on expenditures in Texas school districts, (b) compare similarities and differences in expenditures among property-poor, medium-wealth, and wealthy-districts, (c) analyze spending patterns in light of equalization efforts, and (d) provide useful data to researchers in the area of equalization and adequacy.

The management style being used by school personnel in Texas and across the nation today is predominately that of a bureaucracy. This model was organized around the industrial revolution that was exercising authority at the turn of the century. Writers and researchers have pointed out that such a model is not capable of providing students the knowledge and skills they will need to enter an increasingly demanding society. One management style relatively new to the educational arena today is that of Total Quality Management. This study reports the results of the impact of the training in those principles by measurement of student test scores.

This study investigated the use of interdisciplinary computer-based simulations compared to traditional teaching methods. The academic intrinsic motivation of gifted and non-gifted students was analyzed using a quasi-experimental design, similar to a pretest/posttest design.

Sofia Gubaidulina is one of the leading composers in the contemporary music world. Her compositional interests have been stimulated by the exploration of and improvisation with rare folk and ritual instruments, and by a deep-rooted belief in the mystical properties of music. Gubaidulina is the author of orchestral and choral works, compositions for solo instruments, chamber music, as well as electronics music. Gubaidulina's Piano Sonata sums up the composer's thinking within her piano music, and at the same time projects the development of her spiritual vision within other genres that are to come. The analytical approach in this paper is based on the correlation between each of the elements of the musical material (form, rhythm, sound, etc.) and its contextual meaning in terms of musical dramaturgy. Set-theory is applied to the analysis of motivic components of the work. The traditional form is just the basis for the original intonational structure within a modern musical idiom. Varieties of rhythmic patterns, as well as an unconventional sound production, make this work breath with an impetuous power. The examination of the Sonata's musical language and content should give some insight not only into Guabaidulina's piano music, but also into a consequent development of …

This study seeks to show how Nike, Inc. has effectively followed a strategy to increase the sale of its products that can be characterized as propaganda. Regarding method, this is a qualitative study which applied the propaganda analysis plan developed by Jowett & O'Donnell (1992) to examine and describe (1) Nike's ideology, (2) corporate structure and culture, (3) purpose, (4) targeted audiences, (5) the dissemination of the propaganda, (6) techniques for increasing its effectiveness, (7) existing counterpropaganda and contradictions, and (8) the effectiveness of the campaign. The study provides evidence that Nike engages in propaganda through an organized, systematic, and deliberate attempt to influence the beliefs, attitudes, perceptions, and actions of specific audiences for the purpose of accomplishing fixed objectives.

N-Acylphosphatidylethanoiamine (NAPE) is synthesized in the microsomes of cotton seedlings by a mechanism that is possibly unique to plants, the ATP-, Ca2+-, and CoA-independent acylation ofphosphatidylethanolamine (PE) with unesterified free fatty acids (FFAs), catalyzed by NAPE synthase. A photoreactive free fatty acid analogue, 12-[(4- azidosalicyl)amino]dodecanoic acid (ASD), and its 125I-labeled derivative acted as substrates for the NAPE synthase enzyme.

By measuring interactions among and between anxiety and the independent variables of country of origin, gender, level of education, and age, this study attempted to gain insight into how students from different countries experience anxiety on a U.S. college campus. Results of the Multivariate Analysis of Variance (MANOVA) and the univariate test(ANOVA) indicated that the gender and level of education of the subjects made no significant difference. However, when it came to country of origin, there were significant differences between two of the cultural groups and respective anxiety level. Findings also support a positive correlation between age and anxiety levels, with the youngest participants having the lowest anxiety levels.

A publication by the National Academy of Sciences, USA (1979) outlined some of the research need for a great variety of economically important woody species whose remaining genetic resources need urgently to be collected and conserved. A viable regeneration system was established via tissue and cell suspension culture for Albizia falcataria and A. lebbeck, two important wood yielding leguminous tree species. The culture medium was standardized after several trials to obtain callus from the leaflet explants of these two tree species. The optimum use of casein hydrolysate (w/v) and coconut milk (v/v) in addition to 6-Benzylaminopurine and Indole-3-butyric acid could induce morphogenesis and somatic embryogenesis in the cultured tissue. This reports the first observation on somatic embryogenesis ofA. lebbeck using leaflets as the explants. Scanning Electron Microscopy and histological studies were done on the different stages plant development following standard techniques. Embryogenesis in suspension culture followed regeneration of plantlets in A. lebbeck. In A.falcaaria the regenerative process followed via organogenesis from the shoot buds developed on the leaf explants. After hardening the regenerated plants were transferred to the greenhouse. Some of the trees grew more than 25 feet tall within a few months outside the greenhouse. Karyotype of the three …

This study develops and empirically tests a model of the determinants of federal spending for crime-fighting policies. An inter-disciplinary approach to building the model is utilized that merges ideas from budgeting, policy analysis and criminology. Four factors hypothesized to impact federal spending for the administration of justice are operationalized as eight variables and tested using ordinary least squares regression analysis on time series data. The factors hypothesized to impact federal spending in this area are economic constraints imposed on government spending, the ideological makeup of Congress and the president, the actual crime rate, and the public's attitude toward crime. Five of the eight variables demonstrated statistical significance at the.10 level or better.

Approximately 950,000 Americans die annually from cardiovascular disease. Physical activity is a major risk factor for the development of CVD and a risk factor for stroke. The purpose of this research was to determine whether American adults know how to exercise to achieve health benefits and whether this knowledge is a function of demographics. Items included knowledge of exercise guidelines and knowledge of traditional and non-traditional exercise activities. This information was obtained from 22 questions that were a part of a larger national survey of 2,002 American households. Statistical analyses of this sample, indicate American adults have knowledge which varies by demographic groups. Data revealed that overall the 61+, Less than High School, African-American, Hispanic-American, and Male groups have the least amount of knowledge about exercise. These data can provide health educators with important aspects of exercise knowledge for future health promotions/interventions.

Since the introduction of management practices by the Forest Service to stabilize red-cockaded woodpecker (RCW) populations, the number of cavity trees killed by southern pine beetles (SPB) has increased. A model of the landscape ecology of RCW and SPB in the Homochitto National Forest was created using data collected from the Forest Service and Global Atmospherics. The conclusions of the study were that the RCW and SPB utilize the same type of habitat and the stand hazard maps are an accurate means of determining the locations of SPB infestations. The functional heterogeneity maps created for the SPB and RCW would be useful predictors of future occurrences of either species if complete data were obtained.

The purpose of this study was to determine if extended periods of time out of the training environment has an effect on the retention of training. The rationale for conducting this study was based on the fact that little research has been done in this area. The findings of the study indicated that extensive periods of time out of training do significantly influence the amount of training retained fromone loadmaster course to the other. Additionally, there was a significant relationship between the number of days out of training and the posttest scores. The optimum training break between courses appears to be between 10 and 20 days. Training retention is apparently affected by time.

The lumbar motor activity in isolated spinal cords of 72 postnatal Balb/C mice aged 2, 5, 10 and 21 days (PN2-21) was electroneurographically recorded (ENG) via bilateral ventral roots following treatment with three different concentrations (25, 100 and 200 pM) of the neurotransmitter, 5-hydroxytryptamine (5-HT), i.e., serotonin, to determine its effects on spinal pattern generation.